Éditeur(s) : Université des Antilles. Service commun de la documentation Université de Guyane
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : The Sargassum seaweed [Sargassum natans and S. fluitans] can be classed as a perennial invasive weed problem in all the Caribbean islands. It has been shown to affect fisher-folks, sea bathers, and tourists. It is proving to be costly to remove; in addition to its offensive odour which hampers the clean-up operations. The University of Trinidad and Tobago at the BAFT campus is engaged in series of activities to find suitable uses of the seaweed as animal feed, and as soil medium and ameliorant. This study aimed at evaluating the suitability of the seaweed as a growing media for the production of sweet peppers [Capsicum annuum var. annuum] using different percentage of the weed (0, 25, 50 and 100%) with soil under greenhouse conditions. The crop growth and development and yield were monitored. The results indicated that the plant height [Y pl ht = 16.6 + 0.3 DAT - 0.11 TRT] and leaf number [Y no.lvs = 0.90 + 1.04 DAT - 0.206 TRT] increased as age of the crop increased, but decreased with the increase in percentage of seaweed/ soil mixture. A similar response was observed for the flower buds and fruits numbers and percentage soil mixture. The medium had no effect on the crop transpiration and net photosynthesis rate [PN] , photosynthesis active radiation[PAR] , and stomatal conductance. A nutrient analysis of the medium was also conducted.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16268
V16268

Éditeur(s) : U.S. G.P.O. : For sale by the U.S. G.P.O., Supt. of Docs., Congressional Sales Office U.S. G.P.O. : ( Washington ) For sale by the U.S. G.P.O., Supt. of Docs., Congressional Sales Office
Résumé : (System Details) Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Haiti
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Haïti
Haïti
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United States
Haiti
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United States
Haïti
États-Unis
Haïti
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États-Unis

682100535

Éditeur(s) : Université des Antilles AREBio Groupe de recherche BIOSPHERES : BIOlogie, Sciences Physiques & Humaines pour les énergies Renouvelables, l
Extrait de : 1er colloque international BIOSPHERES, du 18 au 20 juin 2019. Université des Antilles
Description : Volcano tourism plays an important role in the economies of volcanic islands in the Lesser Antilles. On islands like St. Lucia and Dominica these volcanoes have been utilized as geoparks and the areas even being designated as World Heritage Sites. In many of these islands, farming communities also live on the slopes to these volcanoes and with the increased pursuit of geothermal energy the siting of geothermal operations are also located in close proximity. Volcanic activity, therefore, has a significant impact on these islands at a local and national level. Many volcanomonitoring institutions face the financial and human resources challenge of providing ongoing monitoring services to economically burdened small island states. This has often led to the exploration of alternative low-cost monitoring options and strategies to be adopted by monitoring agencies. One such strategy is the adoption of a volcanic risk reduction approach to research and monitoring activities, which integrates the social and physical sciences through the engagement of local communities as partners with scientists in disaster risk reduction. We present a recent approach adopted using this type of strategy in St. Lucia and lessons learnt from this approach with implications for volcano tourism on island. The potential use of this strategy in Dominica is also presented, given the economic challenges the island currently faces during its ongoing recovery efforts from Hurricane Maria in 2017.

Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V19086
V19086

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
In Trinidad and Tobago, cocoa production is monitored by the Cocoa and Coffee Industry Board, which was established in 1961. Cocoa, used to make chocolate, is a traditional export crop in the country. Buildings like those pictured would be used for processing, drying, sorting, and storing cocoa beans. In this image, several children and adults can be found in the yard near buildings on the cocoa plantation. The buildings are made with wood and have zinc paneled roofs, some of the steps are made of concrete. The level flat area between the buildings on the far right would be used to dry cocoa beans. The beans would be spread out evenly on this area, then raked and turned so that beans were exposed to the sun for adequate drying. Slide labeled Cocoa Plantation Trinidad.
Trinidad and Tobago -- South America -- Trinidad
Trinidad and Tobago -- Caribbean region -- Trinidad

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
http://ufdc.ufl.edu/AA00031228/00001

Éditeur(s) : UNU-INWEH UNU-INWEH ( Hamilton, Ontario, Canada )
Résumé : (Internal Comments) Assessment, Monitoring and Management of Persistent Organic Pollutants (POP) and Persistent Toxic Substances (PTS) in the Coastal Ecosystems of the Wider Caribbean Region, 10-12 June 2008, Chaguaramas, Trinidad and Tobago : Report of the workshop

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
A rapid and specific high-performance liquid chromatography method with UV detection (HPLC-UV) for the simultaneous determination of 12 beta-lactam antibiotics (amoxicillin, cefepime, cefotaxime, ceftazidime, ceftriaxone, cloxacillin, imipenem, meropenem, oxacillin, penicillin G, piperacillin, and ticarcillin) in small samples of human plasma is described. Extraction consisted of protein precipitation by acetonitrile. An Atlantis T3 analytical column with a linear gradient of acetonitrile and a pH 2 phosphoric acid solution was used for separation. Wavelength photodiode array detection was set either at 210 nm, 230 nm, or 298 nm according to the compound. This method is accurate and reproducible (coefficient of variation [CV] < 8%), allowing quantification of beta-lactam plasma levels from 5 to 250 μg/ml without interference with other common drugs. This technique is easy to use in routine therapeutic drug monitoring of beta-lactam antibiotics.
ISSN: 0066-4804

hal-00875271
https://hal.archives-ouvertes.fr/hal-00875271
https://hal.archives-ouvertes.fr/hal-00875271/document
https://hal.archives-ouvertes.fr/hal-00875271/file/Simultaneous_Determination-accepted.pdf
PUBMED : 21788467

Éditeur(s) : GFP : Groupe Français des Pesticides
Extrait de : "Protection des cultures et santé environnementale : héritages et conceptions nouvelles" : congrès, le 26 mai 2014. Université des Antilles et de la Guyane
Description : Bien que la réglementation concernant l'usage des phytosanitaires soit de plus en plus encadrée, la présence de nombreux pesticides persiste dans l'environnement. Le lessivage des surfaces cultivées entraine ces derniers dans les écosystèmes aquatiques dans lesquels on trouve encore des molécules comme l'atrazine dont l'emploi est pourtant interdit en Europe depuis plus de 10 ans. La présence de ces molécules constitue un risque avéré pour la pérennité de la ressource en eau. Ainsi, des outils de monitoring pour une surveillance in situ des écosystèmes deviennent indispensables pour les gestionnaires. C'est dans ce contexte que nous développons des biocapteurs dont le fonctionnement repose sur l'étude de la perturbation du métabolisme de cellules algales immobilisées. Les algues, à la base des chaînes trophiques, sont très sensibles aux pollutions. L'outil proposé possède ainsi une bonne pertinence écologique
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V14251
V14251

Éditeur(s) : University of West Indies, Barbados Université des Antilles. Service commun de la documentation
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : A germination bioassay was developed to screen for potential phytotoxicity of soil amendment products from organic waste recycling. In phase 1 of the study, nine soil amendment products were applied as mulches on 3- to 5-week-old tomato seedlings (Solanum lycopersicum L.HA3019?) planted in 4L pots. Plant growth was reduced by the less mature mulch materials. Using lettuce (Lactuca sativa L.New Red Fire?) as the test species, a laboratory germination test was developed in phase 2 with aqueous extracts from a non-phytotoxic (safe) and a phytotoxic product identified in phase 1. Extracts were obtained by soaking 10g of air-dried product in 90 mL distilled water for 24h at 25°C. Diluted extracts (0, 25, 50, 75 and 100%) were applied to lettuce seeds sown on filter paper in petri-dishes and germination was monitored for 5 days, confirming the results from phase 1. In phase 3, the bioassay developed in phase 2 was used to test three previously untested local products and one imported product. Two of the local products were comparable to the imported product. Results obtained in phase 3 were confirmed using the mulch test as described in phase 1. Hence, the germination bioassay can be a useful tool for quality control in the production of(safe) soil amendment products from organic waste recycling.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16302
V16302

Éditeur(s) : EDP Sciences
Résumé : The WFD monitoring requirements for surface waters were adapted to the coastal area with the selection of the relevant parameters to qualify the maritime ecosystems, with appropriate strategies of sampling. Ifremer and Cemagref for the fish indicator in the estuarine waters, are in charge of the elaboration of the monitoring programme in coastal waters (surveillance monitoring and operational monitoring). Ifremer has elaborated technical recommendations for the monitoring of the biological (benthic and phytoplanctonic) and chemical elements, according to many years of observations by the french monitoring networks (RNO, Rephy, Rinbio, RSL, Rebent). These recommendations were established within experts groups They clarify the parameters, the analytical methods, and the strategies of sampling for the classification of the water bodies in five ecological classes and two chemical classes. The work on the relevant indicators of the ecological status still goes on, in relation with the 2006 european intercalibration exercise. Available data and future data will be used in order to set the borders between the classes of high, good, and moderate status, before the monitoring programme starts. Data sets have to be completed to set the borders and to assess the ecological status of water bodies.
Houille Blanche - Revue internationale de l'eau (0018-6368) (EDP Sciences), 2006-07 , N. 4 , P. 68-74

Droits : © Société Hydrotechnique de France 2006
http://archimer.ifremer.fr/doc/2006/publication-3530.pdf
DOI:10.1051/lhb:200604011
http://archimer.ifremer.fr/doc/00000/3530/

Éditeur(s) : HAL CCSD EDP Sciences
Résumé : International audience
In order to ensure a sustainable management of reef fisheries, data need to be collected regularly on the effect of these fisheries on both fish resources and the ecosystems that sustain them. Habitat and resources monitoring programs can provide such information, but are difficult to implement as they involve a range of technical, financial and human resources. Regarding these problems, the involvement of the local population in monitoring programs, called participatory monitoring, seems to offer solutions. Indeed, participatory monitoring has the potential of increasing the number of data collected at low cost, while taking advantage of the traditional ecological knowledge available locally. Some questions need clarifying, however: which methods can bring relevant information for the monitoring of reef fisheries while being appropriate to the motivations and skills of the local population? Is the degree of participation of the local population sufficient to make the monitoring program work, particularly over the long term? To answer these questions, participatory monitoring methods commonly used for coral reef ecosystems have been implemented to assess reef fisheries at three different sites: Moorea island and Tikehau atoll in French Polynesia, and the Bay of Tulear in Madagascar. While highlighting the relevance of some participatory monitoring programs for snapshot and large-scale surveys, the results of this study also show their lesser interest for regular data gathering on long-time scales. In any case, strong coordination and supervision by scientists are considered essential.
La gestion durable des écosystèmes coralliens nécessite de disposer d’informations régulières concernant l’impact des pêcheries sur les ressources récifales et sur les écosystèmes dont elles dépendent. Les suivis intégrés des ressources, des habitats et des espèces associées qui visent à fournir ce type d’informations sont difficiles à mettre en œuvre, car ils impliquent de nombreuses ressources humaines, techniques et financières. Face à ces problèmes, la participation de la société civile dans les programmes de suivi semble en mesure d’apporter des solutions. Dans le but de tester cette hypothèse, des méthodes de suivi participatif ont été mises en œuvre afin de caractériser des pêcheries récifales sur trois socio-écosystèmes contrastés : l’île de Moorea et l’atoll de Tikehau en Polynésie française, et le littoral de Tuléar à Madagascar. Les résultats de cette étude soulignent la pertinence de certains suivis participatifs pour fournir une vision écosystémique dans le cas de collecte de données ponctuelles et de large envergure, et leur intérêt moindre pour la collecte continue de données sur de longues échelles de temps. Dans tous les cas, une structure de coordination et l’implication de scientifiques s’avèrent primordiales pour assurer pérennité et fiabilité au système de suivi.
ISSN: 1240-1307

hal-01302983
https://hal-univ-perp.archives-ouvertes.fr/hal-01302983
DOI : 10.1051/nss/2013109

Éditeur(s) : Wiley-blackwell Publishing, Inc
Résumé : Recent theoretical and field studies conducted in both terrestrial and marine ecosystems have suggested that diversity could be a more complex and multi-component concept than previously thought. However, it is still poorly understood to what extent the information provided by the various indices is complementary with regard to diversity, and to what extent this complementarity is reproducible in different situations or at different scales of observation. In the present work, we have analysed the reproducibility of the empirical relationships between 11 diversity indices related to four major aspects (components) of species diversity (species richness, rarity, evenness and species taxonomy) in order to determine whether there is a general pattern of redundancy or complementarity in diversity measures that holds across years, areas and spatial scales. We have applied this approach to groundfish diversity through the analysis of 2404 trawl hauls collected during the first large-scale annual surveys carried out using a single sampling design throughout the northern Mediterranean Sea (ranging from 36.3 to 45.7 degrees N, and 5.3 degrees W to 28 degrees E). Whatever the years, areas and scales studied, we found a strong reproducibility in the number and the nature of the main complementary components of diversity. The whole set of diversity indices considered may be roughly split into 6 complementary groups of descriptors. This result highlights that, in contrast to what is still the usual practice, monitoring diversity should not be based on only one or two of the most widely used components, even at large scale. Finally, in a context where developing tools for monitoring diversity is considered as a high priority worldwide, the strong reproducibility of the relationships between diversity indices we observed offers a basis for discussion of the feasibility and relevance of proposing a general shortlist of indices for monitoring diversity at different management scales.
Ecography (0906-7590) (Wiley-blackwell Publishing, Inc), 2010-12 , Vol. 33 , N. 6 , P. 1123-1135

Droits : 2010 The Authors / Ecography
http://archimer.ifremer.fr/doc/00029/14064/11382.pdf
DOI:10.1111/j.1600-0587.2009.06259.x
http://archimer.ifremer.fr/doc/00029/14064/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
A shallow field experimental site for CO2 injection was established at Maguelone (Languedoc, France), in order to test in an integrated manner a suite of surface and downhole hydrogeophysical monitoring methods. The objective is to improve monitoring of gas transport in the shallow subsurface and to determine the sensitivity of CO2 monitoring systems for leakage detection. The site offers a natural laboratory to study the processes associated with CO2 injection in a clastic and clay-rich context saturated with saline fluids. Prior to CO2 injection, three nitrogen (N2) injections were undertaken in 2012 to measure the site response to neutral gas injection. In 2013, a volume of 111 m3 (mass of 220 kg) of CO2 was injected during 3.5 h at 15 m depth. During each experiment, the gas plumes were successfully detected from pressure monitoring, time-lapse induction logging and downhole resistivity monitoring with dipole–dipole array. Increases in resistivity are attributed to free gas propagation (either N2 or CO2) whereas decreases in resistivity correlate with CO2 dissolution in the pore fluid. Chemical analyses confirm this hypothesis with a decrease in pH and an increase in the concentration of dissolved species in the latter case.
ISSN: 1750-5836

hal-01355943
https://hal.archives-ouvertes.fr/hal-01355943
DOI : 10.1016/j.ijggc.2015.12.005

Éditeur(s) : HAL CCSD Elsevier
Résumé : Vegetation phenology is the study of the timing of seasonal events that are considered to be the result of adaptive responses to climate variations on short and long time scales. In the field of remote sensing of vegetation phenology, phenological metrics are derived from time series of optical data. For that purpose, considerable effort has been specifically focused on developing noise reduction and cloud-contaminated data removal techniques to improve the quality of remotely-sensed time series. Comparative studies between time series composed of satellite data acquired under clear and cloudy conditions and from radiometric data obtained with high accuracy from ground-based measurements constitute a direct and effective way to assess the operational use and limitations of remote sensing for predicting the main plant phenological events. In the present paper, we sought to explicitly evaluate the potential use of MODerate resolution Imaging Spectroradiometer (MODIS) remote sensing data for monitoring the seasonal dynamics of different types of vegetation cover that are representative of the major terrestrial biomes, including temperate deciduous forests, evergreen forests, African savannah, and crops. After cloud screening and filtering, we compared the temporal patterns and phenological metrics derived from in situ NDVI time series and from MODIS daily and 16-composite products. We also evaluated the effects of residual noise and the influence of data gaps in MODIS NDVI time series on the identification of the most relevant metrics for vegetation phenology monitoring. The results show that the inflexion points of a model fitted to a MODIS NDVI time series allow accurate estimates of the onset of greenness in the spring and the onset of yellowing in the autumn in deciduous forests (RMSE <= one week). Phenological metrics identical to those provided with the MODIS Global Vegetation Phenology product (MDC12Q2) are less robust to data gaps, and they can be subject to large biases of approximately two weeks or more during the autumn phenological transitions. In the evergreen forests, in situ NDVI time series describe the phenology with high fidelity despite small temporal changes in the canopy foliage. However, MODIS is unable to provide consistent phenological patterns. In crops and savannah, MODIS NDVI time series reproduce the general temporal patterns of phenology, but significant discrepancies appear between MODIS and ground-based NDVI time series during very localized periods of time depending on the weather conditions and spatial heterogeneity within the MODIS pixel. In the rainforest, the temporal pattern exhibited by a MODIS 16-day composite NDVI time series is more likely due to a pattern of noise in the NDVI data structure according to both rainy and dry seasons rather than to phenological changes. More investigations are needed, but in all cases, this result leads us to conclude that MODIS time series in tropical rainforests should be interpreted with great caution. (C) 2013 Elsevier Inc. All rights reserved.
ISSN: 0034-4257

hal-01032407
https://hal.archives-ouvertes.fr/hal-01032407
DOI : 10.1016/j.rse.2013.01.010

Résumé : Monitoring n°28 of the Paluel nuclear power plant takes into account studies conducted between March and September 2005. Impacts on hydrology, microbiology, phytoplankton, zooplankton, fisheries, intertidal phytobenthos and zoobenthos are reported. This power plant has been in operation for twenty two years.
L'étude n°28 du site électronucléaire de Paluel (département de la Seine-Maritime) concerne l'année 2005. Sont étudiés les domaines benthique, halieutique et pélagique. L'année 2005 est la 22ème année de fonctionnement de la Centrale.

Droits : 2006 Ifremer
http://archimer.ifremer.fr/doc/2006/rapport-3814.pdf
http://archimer.ifremer.fr/doc/00000/3814/

Éditeur(s) : HAL CCSD
Résumé : Both short-term and long-term morphodynamics of sand barrier systems forced by moderate storm conditions have been studied significantly in the literature. However, the dynamics of the emerged beach -- from a seaside water depth of ~ 1.5 metres below the lowest tide to the back-barrier -- impacted by typhoons remain scarcely documented. This study focuses on the analysis and a better understanding of the morphologic changes of such an emerged beach, when it is forced by one typhoon, several typhoons or the combination of a summer season of typhoons and a winter season of Monsoon wave/ wind conditions. The monitoring of near-shore hydrodynamics and beach morphodynamics was performed in the Wan-Tzu-Liao sand barrier from November 2011 to August 2012. Waves, currents and water level were measured at an offshore buoy (Cigu buoy; 18 m of water depth; 1.5 km seaward the studied zone), a current profiler (4 m of water depth), and a serie of pressure sensors deployed along a cross-shore section from the lower intertidal zone up to the eolian dune. Tide was obtained from a tide gauge inside the lagoon and wind conditions were obtained respectively from an inland station and the offshore Cigu buoy. Morphodynamic changes were monitoring thanks to DGDPS surveys performed on a grid including a 300 meters long portion of sand barrier extending from the subtidal zone to the back-barrier, as well as along two cross-shore and two long-shore profiles located within the grid. Surveys were performed each week during winter and just before and after each storm during the typhoons summer season. This field methodology provided a new and very robust dataset in order to quantify sand barrier dynamics exposed to high-energy events at various time scales. Amongst the eight typhoons recorded, TALIM is the most significant and representative. Offshore wave height reached 10.34 m (Tp = 14.6 s) at Cigu buoy and 2.3 m in 4 m of water depth. Morphological changes recorded 6 m of retreat for the dune front, a 20 m large dune breaching and the occurence of a wash-over fan in the lagoon. These changes form a well-known morphodynamic responses to moderate storms. More surprisingly, TALIM drove a significant nourishment of the supratidal zone, as well as a positive sand balance on the whole sand barrier. Five other typhoons resulted in the same type of morphological responses while two typhoons resulted in a strong erosion of the whole emerged beach. During winter and spring (moderate wave conditions and strong Monsoon winds), sand moved from the beach front to the back-barrier. For the whole year, the sand barrier recorded a 12 m landward migration concomitantly with a 12 m widening without abrasion of the dune elevation.
American Geophysical Union, Fall Meeting 2013

hal-01012312
https://hal.archives-ouvertes.fr/hal-01012312

Éditeur(s) : HAL CCSD MDPI
Résumé : International audience
Microbial pollution in aquatic environments is one of the crucial issues with regard to the sanitary state of water bodies used for drinking water supply, recreational activities and harvesting seafood due to a potential contamination by pathogenic bacteria, protozoa or viruses. To address this risk, microbial contamination monitoring is usually assessed by turbidity measurements performed at drinking water plants. Some recent studies have shown significant correlations of microbial contamination with the risk of endemic gastroenteresis. However the relevance of turbidimetry may be limited since the presence of colloids in water creates interferences with the nephelometric response. Thus there is a need for a more relevant, simple and fast indicator for microbial contamination detection in water, especially in the perspective of climate change with the increase of heavy rainfall events. This review focuses on the one hand on sources, fate and behavior of microorganisms in water and factors influencing pathogens' presence, transportation and mobilization, and on the second hand, on the existing optical methods used for monitoring microbiological risks. Finally, this paper proposes new ways of research.
ISSN: 1660-4601

hal-01122209
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122209
PUBMED : 24747537
DOI : 10.3390/ijerph110404292
PUBMEDCENTRAL : PMC4025003

Éditeur(s) : HAL CCSD
Résumé : The presence of biological matter (biofilms) in deep geological sites for storage of, for instance, toxic elements or groundwater in aquifers was clearly proved. That biomass triggers physical and chemical processes which greatly modify the durability and the sustainability of the sites. These processes, mainly from oxidative/reductive reactions, are poorly understood. This is mainly due to the fact that former studies were done at the macroscopic level far away from the micrometric scale where relevant processes induced by bacteria take place. Investigations at microscopic level are needed. Thus, we developed an experimental set-up based on the combined use of optical microscopy (transmission), atomic force microscopy (AFM) and electrical and electro-chemical AFM microscopy (EC_AFM) in order to get simultaneous information on topographic and electro-chemical processes. The first highly sensitive step was to use AFM with biological samples in liquid environment: wepresent here a study about AFM imaging of living, moving or self-immobilized bacteria, in their genuine physiological liquid medium and in true in vivo conditions. No external immobilization protocol, neither chemical nor mechanical was needed. For the first time, the native gliding movements of Gram negative Nostoc cyanobacteria upon the surface were studied by AFM. AFM height and mechanical stiffness data were simultaneously acquired. From these, mechanical parameters, inner turgor pressure and Young modulus, were derived for different bacterial species (Anabaenopsis circularis, Rhodococcus wratislaviensis). Our study revealed that AFM imaging is thus possible on moving living species. These results open a large window on new studies of both dynamical phenomena of practical and fundamental interests such as the formation of biofilms and dynamic properties of bacteria in real physiological conditions. The second delicate step was to combine AFM and optical measurements with electrical ones. We mounted a new experimental set-up coupling real-time (i) monitoring of optical properties as the optical density (OD) evolution related to bulk bacterial growth in liquid or as the counting of number of bacteria adhering on the surface of the sample as well and (ii) electrical and electrochemical measurements. Furthermore, these results will shortly be applied to the optimized monitoring of the in-situ activity of bacteria consuming oil pollutants, following this way, in real-time, the bioremediation of an oilcontaminated soil (ANR ECOTECH_BIOPHY program).
La présence de matière biologique (biofilms) dans les sites de stockage géologique profond, d'éléments toxiques ou encore de l'eau potable des aquifères est maintenant clairement démontrée. Cette biomasse est à l'origine de processus physiques et chimiques qui modifient considérablement la durabilité et la pérennité des sites concernés. Ces processus, principalement de type oxydo-réductif, sont encore mal compris. Ceci est principalement dû aux méthodes d'investigation, principalement macroscopiques, loin de l'échelle micrométrique caractéristique des bactéries. Seules des études, basées sur des méthodes d'investigation locale, peuvent apporter les informations requises. Ainsi, nous avons développé un dispositif expérimental basé sur l'utilisation combinée de la microscopie optique (en transmission), la microscopie à force atomique (AFM) et la microscopie AFM en mode électrique et électrochimique (EC_AFM) afin d'obtenir des informations simultanées sur la topographie de l'échantillon et sur les processus électrochimiques à l'échelle des bactéries. La première étape sensible consistait à utiliser l'AFM sur des échantillons biologiques en milieu liquide: nous présentons ici les résultats de l'imagerie AFM en milieu liquide de plusieurs types de bactéries dans leurs conditions physiologiques naturelles (conditions in vivo). Aucun protocole d'immobilisation, ni chimique ni mécanique, n'a été nécessaire; et pour la première fois, les mouvements de reptation de cyanobactéries Nostoc ont été étudiés par l'AFM. Les études AFM ont permis d'acquérir des données topographiques mais aussi mécaniques : nous avons pu ainsi mesurer le module d'Young, la pression de turgescence de différentes souches bactériennes (Anabaenopsis circularis, Rhodococcus wratislaviensis). Cette étude complète, a révélé que l'imagerie AFM est donc possible sur des espèces vivantes en mouvement. Ces résultats ouvrent une grande fenêtre sur de nouvelles études d'intérêts tels que la formation de biofilms et les propriétés dynamiques de bactéries dans des conditions physiologiques réelles. La deuxième étape délicate était de combiner l'AFM aux mesures optiques et électriques. Nous avons développé un nouveau dispositif expérimental permettant (i) le suivi de l'évolution de la croissance bactérienne par la mesure des propriétés optiques comme la densité optique DO (pour le développement bactérien en volume - milieu planctonique) , ou l'analyse de l'image du substrat par comptage du nombre de bactéries sur la surface de l'échantillon (biofilm), et (ii) les mesures électriques et électrochimiques. L'ensemble de ces résultats sera prochainement appliqué au développement de nouveaux outils de surveillance d'une biodépollution de terrain contaminé par les hydrocarbures, par le suivi in situ et en temps réel de l'activité de bactéries dépolluantes (ECOTECH_BIOPHY ANR).
https://tel.archives-ouvertes.fr/tel-01044714

tel-01044714
https://tel.archives-ouvertes.fr/tel-01044714
https://tel.archives-ouvertes.fr/tel-01044714/document
https://tel.archives-ouvertes.fr/tel-01044714/file/theseDhahri2013.pdf

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Advances in groundwater storage monitoring are crucial for water resource management and hydrological processes understanding. The evaluation of water storage changes (WSC) often involve point measurements (observation wells, moisture probes, etc.), which may be inappropriate in heterogeneous media. Over the past few years, there has been an increasing interest in the use of gravimetry for hydrological studies. In the framework of the GHYRAF (Gravity and Hydrology inAfrica) project, 3 yr of repeated absolute gravity measurements using a FG5-type gravimeter have been undertaken at Nalohou, a Sudanian site in northern Benin. Hydrological data are collected within the long-term observing system AMMA-Catch. Once corrected for solid earth tides, ocean loading, air pressure effects, polar motion contribution and non-local hydrology, seasonal gravity variations reach up to 11 μGal, equivalent to a WSC of 260-mm thick infinite layer of water. Absolute temporal gravity data are compared to WSC deduced from neutron probe and water-table variations through a direct modelling approach. First, we use neutronic measurements available for the whole vertical profile where WSC occur (the vadose zone and a shallow unconfined aquifer). The RMSD between observed and modelled gravity variations is 1.61 μGal, which falls within the error bars of the absolute gravity data. Second, to acknowledge for the spatial variability of aquifer properties, we use a 2-D model for specific yield (Sy) derived from resistivity mapping and Magnetic Resonance Soundings (MRS). The latter provides a water content (θMRS) known to be higher than the specific yield. Hence, we scaled the 2-D model of θMRS with a single factor (α). WSC are calculated from water-table monitoring in the aquifer layer and neutronic measurements in the vadose layer. The value of α is obtained with aMonte-Carlo sampling approach, minimizing the RMSD between modelled and observed gravity variations. This leads to α = Sy/θMRS = 0.63 ± 0.15, close to what is found in the literature on the basis of pumping tests experiments, with a RMSD value of 0.94 μGal. This hydrogeophysical experiment is a first step towards the use of time-lapse gravity data as an integrative tool to monitor interannual WSC even in complicated subsurface distribution.
ISSN: 0956-540X

hal-00821181
https://hal.archives-ouvertes.fr/hal-00821181
DOI : 10.1093/gji/ggt146

Éditeur(s) : Université de Bretagne Occidentale
Résumé : This activity report entitled (“Chemical contamination of the marine environment: from measurement to risk assessment”) summarizes the main stages my career at Ifremer from November 1975 to june 2013. The first chapter deals with the seawater chlorination and chlorinated waters released into the sea, the identification and the conditions of the formation of various by-product of these processes. The second chapter concerns PCBs, their nature, their chemical analysis in environmental matrices. PCBs are considered as the best examples of persistant and bioaccumulable compounds and therefore, in all this report they are flagship contaminants to which other groups of studied substances are compared. The third chapter refers to studies performed within marine pollution monitoring programme RNO (Réseau National d’Observation de la qualité de l’environnement marin - National observation network on the quality of the marine environnment) and well as other more limited studies like a first assessement on the presence of dioxins and dioxin-like PCBs in the French coastal marine environment and last, on the use of zebra mussel (Dresseinia polymorpha) as sentinelle species of the chemical contamination in the Seine estuary. The next two chapters are about bioaccumulation and about the distribution of organic contaminants (PCBs, dioxins, PAHs, PBDEs) in marine organisms and in marine foodwebs. These studies aim at a better knowledge of the presence and fate of these compounds in fish and marine organisms eitheir in an environmental perspective (chapter IV) or in order to contribute to the assessment of their health impact, or more precisely to estimate therelative part of fish and seafood to the human exposure to chemical residues (chapter V). To conclude, the chapter VI concerns the PCB and dioxin fingerprints in the various studied marine matrices ; analytical and environmental interpretations of the observed differences are suggested as well as possible applications.
Ce bilan d’activité intitulé « Contamination chimique du milieu marin : de la mesure à l’évaluation des risques » retrace les étapes essentielles de mon parcours à Ifremer entre novembre 1975 et juin 2013. Le premier chapitre traite de la chloration dans l’eau de mer et les rejets chlorés en mer, l’identification des sous-produits formés lors de ces traitements, les conditions de leur formation. Le second chapitre concerne la nature des PCB et leur analyse dans les matrices environnementales. Les PCB considérés comme les meilleurs exemples de composés persistants bioaccumulables constituent les « contaminants phares » de l’ensemble de ce travail et c’est par rapport aux PCB que sont comparés et évalués les autres groupes de contaminants étudiés. Le troisième chapitre se rapporte aux travaux réalisés dans le cadre de la surveillance RNO (Réseau National d’Observation de la qualité de l’environnement marin) ainsi que dans des études ponctuelles comme un premier inventaire sur les niveaux de présence des dioxines (PCDD et PCDF) dans l’environnement marin littoral ou sur l’utilisation de la moule zébrée (Dresseinia polymorpha) comme espèce indicatrice de la contamination de l’estuaire de la Seine. Les deux chapitres suivants sont consacrés aux travaux sur la bioaccumulation et aux contaminants organiques (PCB, dioxines, HAP, PBDE) dans les organismes et les réseaux trophiques marins soit dans une perspective environnementale sur le devenir des contaminants dans le vivant (chapitre IV) soit dans le cadre de l’évaluation des risques sanitaires ou, plus précisément, de l’estimation de la contribution de la consommation des produits de la mer à l’exposition de l’homme aux résidus chimiques (chapitre V). Pour terminer, le chapitre VI concerne les empreintes de PCB et de dioxines dans les différentes matrices étudiées ; une interprétation analytique et environnementale des différences observées est proposée ainsi que de possibles applications.

Droits : 2013 UBO
http://archimer.ifremer.fr/doc/00168/27946/26237.pdf
http://archimer.ifremer.fr/doc/00168/27946/

Éditeur(s) : HAL CCSD
Résumé : Karst systems are the most important groundwater reservoirs in Mediterranean areas. Their structure and functioning are complex and not well known because of the high heterogeneity created and organised by groundwater flow. Water storage entities and water storage changes within remain difficult to assess by direct measurement and modelling. Geodetic monitoring is here used to constrain and bring original insights on karst system functioning. The Durzon karst system (Larzac plateau, Southern France) is monitored by several geodetic techniques: - Monthly absolute gravity measurements at three sites - Time-lapse surface to depth gravity measurements - Time-lapse microgravity surveying - Continuous long base hydrostatic tiltmeter monitoring at two sites Absolute gravity monitoring complemented with relative gravity surveys allows quantification of water storage changes on the studied karst system. Interannual water storage changes as detected by gravity are ~400 mm in amplitude. These storage changes are shown to occur in the epikarst and infiltration zone in certain areas. Water storage variations spatial heterogeneity is also demonstrated on the karst system. Tilt measurements monitor fast and slow infiltration processes within the karst fractured media. Karst system geodetic monitoring brings both quantitative and integrated hydrologic knowledge. Such methods could be applied to other karst aquifer studies.
Les aquifères karstiques constituent l'essentiel des ressources en eau souterraine sur le pourtour de la Méditerranée. Leur structure et leur fonctionnement sont complexes et mal connus à cause du processus de karstification qui hiérarchise la circulation hydrodynamique en leur sein. La localisation du stockage de l'eau et de ses variations au sein de tels aquifères est difficile à mesurer et à modéliser. Nous utilisons les outils de la géodésie pour apporter de nouvelles contraintes sur le fonctionnement hydrologique et les variations de stock d'eau en milieu karstique. Ainsi, le système karstique du Durzon, (Causse du Larzac, France) fait l'objet d'un suivi par plusieurs méthodes géodésiques incluant: - Des mesures de gravimétrie absolue en trois sites répétées de manière mensuelle ; - Des mesures répétées de gravimétrie relative entre la surface et la profondeur du karst ; - Des mesures répétées de microgravimétrie ; - Des mesures inclinométriques longue base en deux sites. Nous démontrons que le couplage entre la gravimétrie absolue et relative permet d'appréhender les variations de stock d'eau sur le bassin d'alimentation étudié. Les variations inter-anuelles observées sont de l'ordre de 400 mm, et nous montrons que celles-ci s'opèrent dans certaines zones au sein des horizons supérieurs du karst : épikarst et zone d'infiltration. Une forte hétérogénéité spatiale des variations de stockage est aussi mise en évidence à l'échelle du bassin d'alimentation. Les mesures inclinométriques permettent de suivre les phénomènes d'infiltration rapide et lente dans la masse karstique fracturée. Le suivi géodésique de ce système karstique permet d'apprécier quantitativement et de façon intégrée son fonctionnement hydrologique et pourra être transposé pour l'étude d'autres aquifère karstiques.
https://tel.archives-ouvertes.fr/tel-00404851

tel-00404851
https://tel.archives-ouvertes.fr/tel-00404851
https://tel.archives-ouvertes.fr/tel-00404851/document
https://tel.archives-ouvertes.fr/tel-00404851/file/THESE_jacob.pdf