Éditeur(s) : INRA : Institut National de la Recherche Agronomique Université des Antilles. Service commun de la documentation
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : In the new context of the agro-ecological transition, present agricultural systems will have to produce more and better in a more constraint world. Mixed crop livestock systems (MCLS) represent sound alternative ways to progressively achieve these goals through crop-livestock integration (CLI). CLI exploits the synergies between cropping and livestock systems through organic fertilization with manure or plant association, use of crop residues to feed livestock, .... It offers many opportunities to improve productivity, as well as to increase resource use efficiency and improve the resilience of the whole farming system. In the scientific literature, authors advocate the interest of MLCS and CLI, based on theoretical considerations, modelling and empirical evidence from local case studies. But these studies do not clearly identify the respective roles of diversity of activities and CLI management practices in improving performances at the level of the whole farming system. Our aim was thus to assess CLI at farm scale in a range of MCLS and to explain farm performances by analyzing the combination of activities and the level of integration. We conducted our analyses in Guadeloupe, (French West Indies), where MCLS and CLI are complex but important challenges for local agricultural. In order to analyze CLI in a holistic and systemic way, we applied a method traditionally used in ecology, ecological network analysis, to study the structure, functioning and performance of agrosystems. This method was implemented on a range of Guadeloupian MCLS where CLI practices where identified. Nitrogen was retained to conduct the analysis due to its central role for both animal and vegetal productions. The ENA method allows an estimation of productivity, resilience, efficiency, productivity and self- sufficiency of the flows network. Our results show that 1/ efficiency and resilience appear correlated to the complexity and intensity of the network of flows; 2/ efficiency and resiliency are positively correlated when we consider them in terms of N flows; 3/ in the Guadeloupian context, CLI concerns mainly specific practices, as feeding pigs with a wide range of crop residues and organic fertilization of small market gardens and plots used to grow tubers. But at whole system level, CLI remains rare and mainly depends on conventional management practices as mineral fertilization and animal complementation of some farming activities as sugar cane, cattle breeding and banana. Moreover, the combination of productions implemented determines the potential of CLI and related performances linked to relative N efficiency of each production. Consequently, performance and especially efficiency and self-sufficiency depend more on the nature of the activity than on CLI management practices. There is still a gap between theoretical studies and CLI in practice, even though CLI is more complicated than simple flows and provides other services and functions. Dans le nouveau contexte de la transition agro-écologique, les systèmes agricoles actuels devront produire plus et les améliorer dans plus de monde de contrainte. Les systèmes mélangés de bétail de culture (MCLS) représentent des manières alternatives saines d'atteindre progressivement ces buts par l'intégration de culture-bétail (CLI). Le CLI exploite les synergies entre l'emblavage et les systèmes de bétail par la fertilisation organique avec l'association d'engrais ou d'usine, utilisation des résidus de culture d'alimenter le bétail,?. Il donne beaucoup d'occasions d'améliorer la productivité, aussi bien que d'augmenter l'efficacité d'utilisation de ressource et d'améliorer la résilience du système d'exploitation agricole entier. Dans la littérature scientifique, les auteurs préconisent l'intérêt de MLCS et de CLI, basé sur des considérations théoriques, modeler et des preuves empiriques des études de cas locales. Mais ces études n'identifient pas clairement les rôles respectifs de la diversité des activités et des pratiques de gestion de CLI en améliorant des interprétations au niveau du système d'exploitation agricole entier. Notre but était ainsi d'évaluer le CLI à l'échelle de ferme dans une gamme de MCLS et d'expliquer des interprétations de ferme en analysant la combinaison des activités et du niveau de l'intégration. Nous avons réalisé nos analyses en Guadeloupe, (des Antilles françaises), où MCLS et le CLI sont des défis complexes mais importants pour agricole local. Afin d'analyser le CLI d'une manière holistique et systémique, nous avons appliqué une méthode traditionnellement employée en écologie, analyse réseau écologique, pour étudier la structure, le fonctionnement et l'interprétation des agrosystems. Cette méthode a été appliquée sur une chaîne de Guadeloupian MCLS où des pratiques en matière de CLI où identifiée. L'azote a été maintenu pour réaliser l'analyse due à son rôle central pour les productions animales et végétales. La méthode d'ENA permet une évaluation de la productivité, de la résilience, de l'efficacité, de la productivité et de l'autosuffisance du réseau d'écoulements. Nos résultats prouvent que 1 efficacités et résiliences semblent corrélées avec la complexité et l'intensité du réseau des écoulements ; 2 efficacité et élasticité sont franchement corrélés quand nous les considérons en termes d'écoulements de N ; 3 dans le contexte de Guadeloupian, CLI concerne principalement des pratiques spécifiques, en tant que les porcs de alimentation par un large éventail de résidus de culture et de fertilisation organique de petits jardins du marché et complots employés pour cultiver des tubercules. Mais à au niveau système entier, le CLI demeure rare et dépend principalement des pratiques de gestion conventionnelles en tant que complémentation minérale de fertilisation et d'animal de quelques activités agricoles comme canne à sucre, élevage et banane. D'ailleurs, la combinaison des productions mises en application détermine le potentiel du CLI et des interprétations relatives liés à l'efficacité relative de N de chaque production. En conséquence, l'interprétation et particulièrement l'efficacité et l'autosuffisance dépendent davantage de la nature de l'activité que sur des pratiques de gestion de CLI. Il y a toujours un espace entre les études et le CLI théoriques dans la pratique, quoique le CLI soit plus compliqué que des écoulements simples et fournisse d'autres services et fonctions.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16247
V16247

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
Boats and ocean liners anchored in Saint George’s harbor, Grenada. A yacht with what appears to be the name Sans Souci is flying a United States of America and the Grenadan flag (1967-1974). In 1967 when Grenada became a British Associated State, it adopted a blue-yellow-green flag with a nutmeg in the center. The nutmeg represented Grenada’s position as the “Spice Isle” and is also featured on the Coat of Arms. This flag was used until 1974 when Grenada gained independence. Merchant Vessel (MV) Geestcape, partially pictured, entered service in 1966. In 1935, the Van Geest brothers in Spalding, United Kingdom started a horticultural business. The brothers later expanded their business to include a cargo service to carry horticultural produce, primarily bananas, from the Windward and Leeward Islands to Europe. MV Geestcape is also flying the flag of the British Merchant Navy, a red flag with the Union Jack in the upper left corner. Motor Ship (MS) Renaissance, behind MV Geestcape, was built in 1966. Until 1977, Renaissance operated primarily on the Marseille-Haifa route under Compagnie Navigation de Paquet (Croiseres Paquet), Marseille, France. The cruise liner had many subsequent owners throughout her history, until her disposal in 2010. She is pictured here flying the French flag. In the distance overlooking the harbor is Fort George, Grenada’s oldest fort which was built by the French in 1705. Fort George continues (2013) to function as the headquarters for the Royal Grenada Police Force, which it has housed since 1854 when the Colonial Government took over control of the fort. Slide labeled Grenada Harbor.
Grenada -- Caribbean region -- Saint George's, Saint George

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
Sheet 44:9
http://ufdc.ufl.edu/AA00029627/00001

Éditeur(s) : INRA : Institut National de la Recherche Agronomique Université des Antilles. Service commun de la documentation
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : In the Global South, improvement of agricultural outputs is eagerly awaited. While by 2050, its population will double areas devoted to agriculture will decrease exacerbating undernutrition of the poor. Unfortunately, the demand for fresh locally-produced meat products is not satisfied yet in the tropics. So, efficiency in animal productions is essential to allow coverage of protein nutritional needs of people, both in quality and quantity. In the Caribbean territories mixed farming systems are the most common farming systems (about 80%) and can constitute a solution to reach food sovereignty in such limited and isolated spaces. Lessons to be learned from these systems involve improving animal performance while respecting the natural balance with environment and maintaining the multi-functionality of plants and animals. Firstly, the natural (or selected) comfort zone of animal and plants must fit with the farm conditions, insuring thereby animal survival and welfare. Enhancement of adaptation to stresses (biotic, abiotic and socio- economic) in species or animal genotypes is a key element implying their equilibrium with the farm environment. This approach underpins for the farmer an integrated management of animal health, nutrition, genetics, reproduction, in close relationship with other compartments of the farming system. Secondly, animal adaptation may be completed by resilience ability within systems. Animals have to produce although facing stresses. Finally, the animal must be efficient that is to say must reconcile physiological functions of production, reproduction with adaptation functions. This optimization leads to decreased inputs and to overall efficiency of mixed farming systems at the end. The aim of the breeder is to choose the animal producing the best balance between output-reproduction-adaptation, at the individual or the flock scale combining genetic and physiological diversity. The underlying idea is to give to humans and animals their right place in the food chain taking into account the farmers? skills and wills. This idea is included in the agroecological approach and may give guidelines for food sovereignty worldwide.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16318
V16318

Éditeur(s) : Université des Antilles AIHP-GEODE : Archéologie Industrielle, Histoire, Patrimoine/Géographie- Développement Environnement de la Caraïbe HARCMAR : Histoire et Archéologie Maritimes des Petites Antilles
Extrait de : "Entre exclusif et contrebande, le commerce colonial aux Antilles XVIIe-début XIXe siècle" : journées d'étude, du 24 au 26 octobre 2019. Université des Antilles
Description : In the eighteenth and the early nineteenth centuries, inter-imperial connections, many of which were illicit, were of crucial importance in the functioning of European colonialism in the Atlantic world and during its heyday. One example of economically important trade connections across imperial borders is the maritime mule trade. Based on a large variety of secondary sources, supplemented by primary materials, this paper seeks to provide insights into the functioning of this business. Enterprising merchants used the opportunities available to them to connect breeding areas and zones of cash crop production. Likewise, geography played an important role in connecting demand and supply over both short and long distances, as physical proximity and trade winds helped forge connections. Mules were transported to the insular Caribbean, and in particular the French islands, to serve as draft animals, especially by moving vertical cylinders called rollers that crushed the sugar cane, although they were not the only animals used for such purposes. To meet the mule demand of the Caribbean islands, the animals were imported from a variety of regions, including France, North Africa, and the Cape Verde islands. Eventually, however, supplies from other parts of the Greater Caribbean came to dominate the mule business.
Siècle(s) traité(s) : 18
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V19112
V19112

Éditeur(s) : Springer
Résumé : The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a rapid bacterial production system to obtain recombinant og TGF-beta protein, and we demonstrate that og TGF-beta is processed by furin to a mature form of the protein. This mature form can be detected in vivo in the gonad. Functional inhibition of mature og TGF-beta in the gonad was conducted by inactivation of the protein using injection of antibodies. We show that inhibition of og TGF-beta function tends to reduce gonadic area. We conclude that mature og TGF-beta probably functions as an activator of germ cells development in oyster.
Marine Biotechnology (1436-2228) (Springer), 2011-10 , Vol. 13 , N. 5 , P. 971-980

Droits : Springer Science+Business Media, LLC 2011
http://archimer.ifremer.fr/doc/00042/15371/13438.pdf
DOI:10.1007/s10126-010-9361-4
http://archimer.ifremer.fr/doc/00042/15371/

Éditeur(s) : HAL CCSD
Résumé : This work is devoted to the theoritical research and to the numerical calculus of weak solutions (in the sens of generalized functions) for the non linear transport equation((∂u)/(∂t))(x,y,t)+a(x,y,t)((∂f(u))/(∂x))(x,y,t)+b(x,y,t)((∂g(u))/(∂y))(x,y,t)=0 pour t>0with the initial condition u(x,y,0)=u₀(x,y) where the functions {(x,y,t)→a(x,y,t)} and {(x,y,t)→b(x,y,t)} belong to L^{∞}(R²×R⁺) (but can be discontinuous), the functions f and g are smooth and monotonous, the function {(x,y)→u₀(x,y)} belongs to L^{∞}(R²). We recall the necessary notions on nonlinear generalized functions for introducing their tensorial product. The main results (to determine the weak solutions) are sufficient conditions so that, when a sum of généralized functions (like Heaviside or Dirac products) is associated with zero, each terms of the sum is equal to zero. Thanks to these theoretical results, we can solve the Riemann problem with the help of a solver written like tensorial product of Heaviside functions (or like a sum of tensorial product of Heaviside functions) in order to obtain the weak solutions. These weak solutions allow to develop two dimensional numerical Godunov type schemes. Then, numerical tests are performed which give a comparison between the results obtained by these 2D schemes and the ones of the splitting method. These tests prove that the 2D numerical schemes are as reliable as the ones obtained by splitting. They are also more simple in their expression. Moreover, a more detailed comparative study of the two types of numerical schemes show that the 2D schemes are far less expensive in the linear case as well as in the non linear case. They are stable for the L^{∞} norm, unlike the splitting schemes. At least some perspectives as well as an existence theorem of a strong solution "almost eveywhere" for linear hyberbolic systems are studied.
Nous nous intéressons à la recherche et au calcul numérique de solutions faibles (au sens des fonctions généralisées) de l'équation de transport non linéaire((∂u)/(∂t))(x,y,t)+a(x,y,t)((∂f(u))/(∂x))(x,y,t)+b(x,y,t)((∂g(u))/(∂y))(x,y,t)=0 pour t>0complétée de la condition initiale : u(x,y,0)=u₀(x,y) où les fonctions {(x,y,t)→a(x,y,t)} et {(x,y,t)→b(x,y,t)} appartiennent à L^{∞}(R²×R⁺) (mais peuvent être discontinues), les fonctions f et g sont lisses et monotones, la fonction {(x,y)→u₀(x,y)} appartient à L^{∞}(R²). Des rappels sur les fonctions généralisées nous permettent d'introduire leur produit tensoriel. Un des résultats clés (pour déterminer ultérieurement les solutions faibles cherchées) donne des conditions suffisantes pour que, lorsqu'une somme de fonctions généralisées (de type produit d'Heaviside ou de Dirac) est associée à 0, chacun des termes de la somme est nul. Grâce à ces résultats théoriques, on résout le problème de Riemann 2D à l'aide d'un solveur s'écrivant comme produit tensoriel de fonctions type Heaviside (ou comme somme de produit tensoriel de fonctions type Heaviside) afin d'obtenir les solutions faibles. Ces solutions faibles permettent la construction des schémas numériques de type Godunov 2D. Nous les validons par des test numériques comparant les résultats obtenus par ces schémas 2D et ceux de la méthode du splitting. Ces tests montrent que les schémas numériques 2D sont aussi fiables que ceux par splitting, alors qu'ils sont plus simples dans leur écriture. Une étude comparative plus complète entre les deux types de schémas numériques montre de plus que les schémas 2D sont nettement moins coûteux à la fois dans les cas linéaire et non linéaire et qu'ils sont stables pour la norme L^{∞}, contrairement aux schémas par splitting. Enfin des perspectives ainsi qu'un théoréme d'existence de solutions fortes "presque partout" pour des systèmes hyperboliques linéaires sont abordés.
https://hal.archives-ouvertes.fr/tel-01487366

Droits : info:eu-repo/semantics/OpenAccess
tel-01487366
https://hal.archives-ouvertes.fr/tel-01487366
https://hal.archives-ouvertes.fr/tel-01487366/document
https://hal.archives-ouvertes.fr/tel-01487366/file/th%C3%A8se_olivier_jouannelle_HAL.pdf

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Are bisphenol A (BPA) and BPA analogs (BPA-A) safe for male human reproductive function? The endocrine function of human testes explants [assessed by measuring testosterone and insulin-like factor 3 (INSL3)] was impacted by exposure of the human adult testis explants to BPA/BPA-A. The few epidemiologic studies performed suggest that bisphenols have potential endocrine disruptive properties, but they did not identify clear and direct patterns of endocrine disruption. Adult human testis explants in culture were exposed to BPA and the analogs bisphenol F (BPF), bisphenol S (BPS), bisphenol E (BPE), bisphenol B (BPB) and bisphenol A diglycidyl ether (BADGE) at 10(-9)-10(-5) M for 24 or 48 h. Human adult testes were obtained from prostate cancer patients who had no hormone therapy, or from multiorgan donors. After ex vivo exposure to the investigated bisphenols, the measured outcomes were related to histopathology (gross morphology and germ cell viability determined by anti-caspase three immunohistochemistry), and the levels of testosterone, INSL3 and inhibin B were measured using immunoassays. The levels of mRNA encoding key enzymes of bisphenol biotransformation were investigated by quantitative PCR: UGT2B15 UDP (glucuronosyltransferase two family, polypeptide B15), GUSB (glucuronidase beta), SULT1A1 and 3 (sulfotransferase family 1 A member 1 and 3) and STS (steroid sulfatase). A significant dose-dependent inhibition was found between testosterone levels measured in the culture medium and concentrations of BPA (P = 0.00778 at 24 h and P = 0.0291 at 48 h), BPE (P = 0.039) and BPF (P = 0.00663). The observed BPA and BPA-A-induced inhibition of testosterone production varied according to duration of exposure and BPA/BPA-A concentrations. BPA (10(-9) M; P < 0.05), BPB (10(-9) M; P < 0.05), BPS (10(-9) and 10(-8) M; P < 0.05) and BADGE (10(-5) M; P < 0.05) increased Leydig cell INSL3 production. By contrast, BPE dose dependently inhibited INSL3 (P = 0.0372). Conversely, Sertoli cell function (inhibin B) and germ cell viability were not significantly affected by either bisphenols. N/A. Environmental compounds cannot be deliberately administered to men, justifying the use of an ex vivo approach. A relatively low number of testes samples were available for analysis (n = 3, except for testosterone secretion with n = 5). The active concentrations of BPA and BPA-A used in the study were higher than those found in human biological fluids. Under our experimental conditions, direct exposure to BPA or BPA-A can result in endocrine disturbance in the adult human testis.
ISSN: 0268-1161

hal-01578582
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01578582
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01578582/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01578582/file/Parallel%20assessment%20of%20the%20effects%20of%20bisphenol%20A_accepted.pdf
DOI : 10.1093/humrep/dex093

Éditeur(s) : The Oceanography Society
Résumé : Data assembly and processing centers are essential elements of the operational oceanography infrastructure. They provide data and products needed by modeling and data assimilation systems; they also provide products directly usable for applications. This paper discusses the role and functions of the data centers for operational oceanography. It describes some of the main data assembly centers (Argo and in situ data, altimetry, sea surface temperature) developed during the Global Ocean Data Assimilation Experiment. An overview of other data centers (wind and fluxes, ocean color, sea ice) is also given. Much progress has been achieved over the past 10 years to validate, intercalibrate, and merge altimeter data from multiple satellites. Accuracy and timeliness of products have been improved, and new products have been developed. The same is true for sea surface temperature data through the Global High-Resolution Sea Surface Temperature Pilot Project. A breakthrough in processing, quality control, and assembly for in situ data has also been achieved through the development of the real-time and delayed-mode Argo data system. In situ and remote-sensing data are now systematically and jointly used to calibrate, validate, and monitor over the long term the quality and consistency of the global ocean observing system. Main results are illustrated. There is also a review of the development and use of products that merge in situ and remote-sensing data. Future issues and main prospects are discussed in the conclusion.
Oceanography (1042-8275) (The Oceanography Society), 2009-09 , Vol. 22 , N. 3 , P. 56-69

Droits : 2009 The Oceanography Society
http://archimer.ifremer.fr/doc/2009/publication-6879.pdf
http://archimer.ifremer.fr/doc/00000/6879/

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : While Amazonian forests are extraordinarily diverse, the abundance of trees is skewed strongly towards relatively few 'hyperdominant' species. In addition to their diversity, Amazonian trees are a key component of the global carbon cycle, assimilating and storing more carbon than any other ecosystem on Earth. Here we ask, using a unique data set of 530 forest plots, if the functions of storing and producing woody carbon are concentrated in a small number of tree species, whether the most abundant species also dominate carbon cycling, and whether dominant species are characterized by specific functional traits. We find that dominance of forest function is even more concentrated in a few species than is dominance of tree abundance, with only ≈1% of Amazon tree species responsible for 50% of carbon storage and productivity. Although those species that contribute most to biomass and productivity are often abundant, species maximum size is also influential, while the identity and ranking of dominant species varies by function and by region.
ISSN: 2041-1723

hal-01204225
https://hal.archives-ouvertes.fr/hal-01204225
DOI : 10.1038/ncomms7857
PRODINRA : 305614
PUBMED : 25919449

Éditeur(s) : HAL CCSD
Résumé : Spermatogenesis in mammals is a complex biological function including cellular processes such as proliferation, meiosis and differentiation, aiming to the production of male gametes in the testis. If the seminiferous epithelium is well described in terms of organization and cellular morphology of cells that compose it, the processes by which undifferentiated diploid germ cells enter meiosis and give haploid cells that undergo many morphological transformations, are not fully decrypted. These processes rely on the coordinated and sequential expression of genes, including specific products for each stage of germ cell development These gene products are essential at each key stage of spermatogenesis. Transcriptomics since the 1990s, and proteomics since the 2000s have contributed to the improved. understanding of these mechanisms. A long term proteomic study aiming at characterizing the proteomes of Sertoli cells and germ cells, and a recent study that characterized and quantified the transcriptome of isolated rat testicular cells at high resolution using de novo sequencing of transcripts (RNA-Seq), have been the basis of my thesis work. The latter study showed the accumulation of long non-Coding RNAs (lncRNAs) and testicular unannotated transcripts (TUTs) at meiotic and post-Meiotic stages of spermatogenesis in the rat. In this context, my thesis work aimed at validating the coding potential of many genes expressed in germ cells using RNA-Seq combined with shotgun proteomics, a so-Called PIT (Proteomics Informed by transcriptomics) approach. In this approach, the protein sequences translated from the transcripts assembled by RNA-Seq in the different testicular cell types are integrated into a custom database of protein sequences used to query mass spectrometry data obtained from proteins of meiotic and post-Meiotic cells. The PIT approach showed that 69 TUTs or lncRNA (corresponding to 44 loci) code for proteins in meiotic cells and post meiotic cells, and we confirmed experimentally the meiotic and post-Meiotic expression for two new transcripts encoding for VAMP9, a protein of the SNARE family, and a new testicular enolase T-ENOL. The post-Meiotic expression of T-ENOL protein was confirmed by immunohistochemistry using a polyclonal antibody raised against the recombinant protein. This approach also allowed us to identify new isoforms of known proteins, specific to each stage of spermatogenesis. Germ cells and Sertoli cells maintain a dialogue which is necessary to the success of spermatogenesis and spermiogenesis. Another part of my work aimed at identifying membrane proteins, in germ cells and residual bodies, that may be involved in the dialogue between Sertoli cells and germ cells, using a ICPL relative quantification proteomic approach. The ICPL analysis enabled us to establish a list of 166 proteins whose expression is differential between pachytene spermatocytes, round spermatids and residual bodies. Their differential expression suggests that these proteins may play a role in spermiogenesis. Thanks to the Gene Ontology annotations, a list of 8 proteins with a putative role in signal transduction, cell recognition or differentiation, thus potentially involved in the dialogue between Sertoli and germ cells was drawn. In addition, I provided a first proteome of rat Sertoli cells, germ cells and residual bodies obtained by shotgun proteomics.
La spermatogenèse chez les mammifères est une fonction biologique complexe incluant des processus de prolifération cellulaire, de méiose et de différenciation uniques visant à la production des gamètes mâles au sein du testicule. Si l’épithélium séminifère est bien décrit sur le plan de son organisation et de la morphologie des cellules qui le composent, les processus par lesquels les cellules germinales diploïdes indifférenciées entrent en méiose pour donner ensuite des cellules haploïdes subissant par la suite de nombreuses transformations morphologiques, ne sont pas totalement décryptés. Ils reposent sur l’expression coordonnée et séquentielle de gènes dont les produits spécifiques de chaque stade de développement des cellules germinales sont essentiels aux étapes clés de la spermatogenèse. La transcriptomique depuis les années 1990 et la protéomique depuis les années 2000 ont contribué à l’amélioration de la connaissance de ces mécanismes. Une étude protéomique visant à caractériser par des approches systématiques et différentielles les protéomes des cellules de Sertoli et de la lignée germinale, et d’autre part une étude récente, réalisée dans notre unité, qui a permis de caractériser et de quantifier le transcriptome des cellules testiculaires isolées de rat en utilisant le séquençage de novo des transcrits (RNA-Seq), ont été à la base de mes travaux de thèse. Cette dernière étude a mis en évidence l’accumulation de longs ARNs non codants (lncRNAs) et de transcrits testiculaires non annotés (TUTs) aux stades méiotique et post- méiotique de la spermatogenèse chez le rat. Dans ce contexte, mon travail a consisté à valider le potentiel codant de nombreux gènes exprimés dans les cellules germinales par une approche dite PIT (Proteomics Informed by Transcriptomics) couplant protéomique Shotgun et RNA-Seq. Dans ce type d’approche, les séquences protéiques déduites des transcrits des différents types cellulaires, assemblés par RNA-Seq, sont intégrées dans une base personnalisée de séquences protéiques utilisée pour interroger les données de spectrométrie de masse obtenues à partir de protéines de cellules méiotiques et post-Méiotiques. L’approche PIT a permis de montrer que 69 TUTs ou lncRNA (correspondant à 44 loci) codent pour des protéines dans les cellules méiotiques et post méiotiques. L’expression post-Méiotique de deux nouveaux transcrits, l’un codant pour la protéine VAMP9, une protéine de la famille SNARE, et l’autre pour une nouvelle énolase T-ENOL a pu être confirmée. L’expression post-Méiotique de T-ENOL a été confirmée par immunohistochimie à l’aide d’un anticorps polyclonal produit contre la protéine recombinante. Cette approche nous a également permis d’identifier de nouvelles isoformes de protéines connues spécifiques de chaque stade de la spermatogenèse. Les cellules germinales et les cellules de Sertoli entretiennent le dialogue nécessaire au bon déroulement de la spermatogenèse. Une autre partie de mon travail a consisté à identifier des protéines membranaires des cellules germinales et des corps résiduels, susceptibles d’intervenir dans le dialogue entre les cellules de Sertoli et les cellules germinales, par une approche protéomique de quantification relative ICPL. Cette approche a permis d’établir une liste de 166 protéines différentiellement exprimées entre les spermatocytes pachytène, les spermatides rondes et les corps résiduels, qui sont susceptibles de jouer un rôle dans la spermiogénèse. Grâce aux annotations de le Gene Ontology, j’ai pu établir une liste de 8 protéines ayant un rôle supposé dans la transduction du signal, la reconnaissance cellulaire ou bien la différenciation. Par ailleurs, j’ai pu établir par protéomique Shotgun un premier protéome des cellules de Sertoli, des cellules germinales et des corps résiduels chez le rat.
https://tel.archives-ouvertes.fr/tel-01127389

Droits : info:eu-repo/semantics/OpenAccess
NNT : 2014REN1S064
tel-01127389
https://tel.archives-ouvertes.fr/tel-01127389
https://tel.archives-ouvertes.fr/tel-01127389/document
https://tel.archives-ouvertes.fr/tel-01127389/file/2014REN1S064.pdf

Éditeur(s) : HAL CCSD
Résumé : This paper studies the biological and economic effects of globalwarming on the French Guiana shrimp …fishery. The sea surface tem-perature is explicitly introduced into four natural growth functions,among which the Cobb-Douglas function best adjusts the availabledata. Besides, a Cobb-Douglas harvest function is also estimated, in-dicating that shrimp production in French Guiana is highly sensitive tothe shrimp stock, which implies that global warming may have strongeconomic implications. We …nally consider a centralized resource man-agement of the French Guiana shrimp …shery, that is undertaken invarious trend scenarios concerning the sea surface temperature. Un-der the most plausible scenario, in which the sea surface temperaturefollows the trend of the last decades, pro…ts and biomass respectivelydecrease and collapse around the end of the 2020’s.
https://hal.univ-antilles.fr/hal-01243305

hal-01243305
https://hal.univ-antilles.fr/hal-01243305
https://hal.univ-antilles.fr/hal-01243305/document
https://hal.univ-antilles.fr/hal-01243305/file/Diop%20et%20al.%20%282015%29.pdf

Éditeur(s) : Elsevier
Résumé : A coupled three-dimensional physical-biogeochemical model was developed in order to simulate the ecological functioning of the Rio de la Plata estuary and plume. The biogeochemical model reproduces the nitrogen cycle between five compartments: dissolved inorganic nitrogen, phytoplankton, zooplankton, detritus and dissolved organic nitrogen. The coupling is tested in seasonal climatological configurations and for the particular year 1999. The circulation is forced with Parana and Uruguay rivers discharges, NCEP wind and tide. The biogeochemical model includes loads of inorganic and organic nitrogen from both rivers. The model reproduces the correct tidal amplitudes in the estuary, as well as the most outstanding features of the observed horizontal and vertical structures of the salinity plume. Simulated surface chlorophyll a concentrations exhibit maximum values all year long seaward of the turbidity front, between the 0.5 and 15 isohalines, in agreement with SeaWiFS images of the area. The model simulates well the low primary production in the light-limited highly turbid tidal river (20 gC/m(2)/yr), the high production area in the frontal zone where it can reach 500 gC/m(2)/yr, and the nutrient-limited production in the outer estuary and inner shelf (300 gC/m(2)/yr), with realistic values in each case. According to the 1999 model simulation, the tidal river is the location of organic nitrogen remineralization with a consequent increase of the inorganic pool. At the entrance of the frontal zone, inorganic nitrogen represents about 75% of the whole nitrogen pool, it is reduced to 50% at its sea end-member. The outer estuary has the same sink role for inorganic nitrogen, suggesting that organic nitrogen is the major form exported to the shelf.
Continental Shelf Research (0278-4343) (Elsevier), 2005-03 , Vol. 25 , N. 5-6 , P. 629-653

Droits : 2004 Elsevier Ltd.
http://archimer.ifremer.fr/doc/2005/publication-334.pdf
DOI:10.1016/j.csr.2004.10.003
http://archimer.ifremer.fr/doc/00000/334/

Éditeur(s) : HAL CCSD Society for Range Management
Résumé : Grasslands and agroecosystems occupy one-third of the terrestrial area, but their contribution to the global carbon cycle remains uncertain. We used a set of 316 site-years of CO(2) exchange measurements to quantify gross primary productivity, respiration, and light-response parameters of grasslands, shrublands/savanna, wetlands, and cropland ecosystems worldwide. We analyzed data from 72 global flux-tower sites partitioned into gross photosynthesis and ecosystem respiration with the use of the light-response method (Gilmanov, T. G., D. A. Johnson, and N. Z. Saliendra. 2003. Growing season CO(2) fluxes in a sagebrushsteppe ecosystem in Idaho: Bowen ratio/energy balance measurements and modeling. Basic and Applied Ecology 4:167-183) from the RANGEFLUX and WORLDGRASSAGRIFLUX data sets supplemented by 46 sites from the FLUXNET La Thuile data set partitioned with the use of the temperature-response method (Reichstein, M., E. Falge, D. Baldocchi, D. Papale, R. Valentini, M. Aubinet, P. Berbigier, C. Bernhofer, N. Buchmann, M. Falk, T. Gilmanov, A. Granier, T. Grunwald, K. Havrankova, D. Janous, A. Knohl, T. Laurela, A. Lohila, D. Loustau, G. Matteucci, T. Meyers, F. Miglietta, J.M. Ourcival, D. Perrin, J. Pumpanen, S. Rambal, E. Rotenberg, M. Sanz, J. Tenhunen, G. Seufert, F. Vaccari, T. Vesala, and D. Yakir. 2005. On the separation of net ecosystem exchange into assimilation and ecosystem respiration: review and improved algorithm. Global Change Biology 11: 1.424-1439). Maximum values of the quantum yield (alpha = 75 mmol.mol(-1)), photosynthetic capacity (A(max) = 3.4 mg CO(2) . m(-2).s-1), gross photosynthesis (P(g,max) = 1.16 g CO(2) . m(-2).d(-1)), and ecological light-use efficiency (epsilon(ecol) = 59 mmol . mol(-1)) of managed grasslands and high-production croplands exceeded those of most forest ecosystems, indicating the potential of nonforest ecosystems for uptake of atmospheric CO(2). Maximum values of gross primary production (8 600 g CO(2) . m(-2).yr(-1)), total ecosystem respiration (7 900 g CO(2) . m(-2).yr(-1)), and net CO(2) exchange (2 400 g CO(2) . m(-2).yr(-1)) were observed for intensively managed grasslands and high-yield crops, and are comparable to or higher than those for forest ecosystems, excluding some tropical forests. On average, 80% of the nonforest sites were apparent sinks for atmospheric CO(2), with mean net uptake of 700 g CO(2) . m(-2).yr(-1) for intensive grasslands and 933 g CO(2) . m(-2).d(-1) for croplands. However, part of these apparent sinks is accumulated in crops and forage, which are carbon pools that are harvested, transported, and decomposed off site. Therefore, although agricultural fields may be predominantly sinks for atmospheric CO(2), this does not imply that they are necessarily increasing their carbon stock.
ISSN: 1550-7424

hal-01190754
https://hal.archives-ouvertes.fr/hal-01190754
DOI : 10.2111/rem-d-09-00072.1
PRODINRA : 214043

Éditeur(s) : Université de la Rochelle
Résumé : In order to better understand the functioning of the Brouage intertidal mudflat (Marennes- Oléron Basin, France), its carbon-based trophic web has been modelled and analyzed. The foodweb building is based on the 4 step method of Inverse Analysis: 1) conceive an a priori model as the graph of compartments (nodes) between which exist fluxes of material (vertices). These vertices are the unknowns for the problem; 2) gather all the existing knowledge about the ecosystem and translate it into linear equations and inequalities involving the fluxes; 3) complete this set of data by common knowledge on the behaviour of the compartments and translate it into inequalities, 4) solve the obtained linear system under the parsimony principle to find a unique solution vector. The food-web analysis is based on. The first purpose of this work was a methodological one: inverse analysis method has been adapted to consider the seasonal and spatial variability of the temperate ecosystem: two seasons have been coupled in the computation and the area has been divided into three geographical zones along a cross-shore gradient. Hence, two types of physical exchanges are involved: the benthicpelagic ones and the advection ones. Statistical results can also be obtained by coupling Monte Carlo methods to Inverse Analysis. To study the obtained networks, new indices based on Markov Chains take delay into account in describing integrative transfers of material between compartments. This lets emerge the systems' properties that are not obvious at first sight and emphasizes the role of peculiar components. The second purpose was to apply such methods to the Brouage mudflat ecosystem in order to better understand its functioning. As the knowledge and methods evolve, various food webs were computed and analyzed with the help such indices and existing ones. Sensitivity analyses allow to point at the most crucial lacks of knowledge, which can orientate future field research. New data would improve the quality of the model in two ways: one the one hand, they would validate or invalidate the outcomes and on the other hand, they would give useful information to better constrain some of the black boxes and most uncertain fluxes, especially the advection and vertical flows. The main results of the study show a strongly seasonal system in which benthic and pelagic components are linked with intensity. The system is driven by its high local benthic primary production, but also depends on imports from the basin. Each of the 3 geographic zones has a peculiar functioning. The central zone corresponds to the classical description of European intertidal mudflats: very productive, it can feed the other regions. It is a production zone. The upper part is less productive but is fed by the middle one via the water column and can be exploited by shorebirds. It is a degradation zone (material is transformed for less qualitative). The lower part strongly depends on the basin imports for its functioning, as the cultivated bivalves act as a pump for pelagic material of which a great part is deposited under the cultivation structures (oyster racks or mussels "bouchots"). It is a transformation zone.
Un double objectif (méthodologique et appliqué) a été visé par une modélisation du réseau trophique de la vasière de Brouage (Bassin de Marennes Oléron, France) à l'aide de l'analyse inverse, et son étude à l'aide de l'analyse des réseaux. L'Analyse Inverse permet en effet de reconstruire l'ensemble du réseau à partir de données éparses, le problème du manque de données étant récurrent en Ecologie. Au plan méthodologique, l'analyse inverse a été adaptée à la prise en compte des caractères saisonnier et spatialement hétérogène d'un écosystème tempéré de zone intertidale : deux saisons ont ainsi été couplées et la vasière a été considérée le long d'un transect perpendiculaire au rivage et divisé en trois zones spatiales. Deux types de flux physiques interviennent donc : les flux verticaux de dépôt et remise en suspension, et les flux d'advection via la colonne d'eau. Des pistes d'amélioration et de nouvelles méthodes sont proposées. Un résultat sous forme statistique peut être obtenu en couplant l'utilisation de méthodes de Monte Carlo à l'analyse inverse pour la construction des réseaux trophiques. Les moyens d'étude des modèles ont été enrichis par la mise au point d'indices issus de la construction de chaînes de Markov et prenant en compte le caractère temporel des transferts de matière entre compartiments. Enfin, la transformation mathématique du modèle statique pour une étude dynamique de la stabilité de l'équilibre décrit est abordée. Plusieurs modèles ont été construits au cours de l'évolution des méthodes et de l'acquisition de connaissances sur le site. Des méthodes d'analyses existantes et d'autres mises au point dans le cadre de cet ouvrage ont été appliquées aux réseaux obtenus afin de faire émerger les propriétés du fonctionnement de la vasière et de souligner le rôle de certains compartiments. Des analyses de sensibilité permettent d'évaluer la robustesse des résultats et d'orienter les recherches futures en mettant en évidence les manques les plus cruciaux. Des données supplémentaires, notamment concernant les foraminifères benthiques, le zooplancton ou les mouvements verticaux du microphytobenthos permettront de fournir de meilleures contraintes et de valider (ou non) les résultats obtenus. Les principaux résultats montrent un système au fort caractère saisonnier dont les compartiments benthiques et pélagiques sont très liés. La production primaire benthique locale est un moteur important, mais qui ne suffirait pas à suppléer l'indispensable importation de matériel détritique. Le découpage de la vasière en trois zones fait apparaître les particularités de chacune. La zone centrale correspond à la description classique des « vasières intertidales européennes » : très productive, elle peut alimenter les autres régions. C'est une zone de production et d'exportation. La partie supérieure est moins productive et reçoit de la matière de la zone de milieu d'estran via la colonne d'eau. Sa production secondaire peut alors être exploitée par les limicoles, nombreux en hiver. C'est une zone de dégradation et d'exportation. Le bas d'estran dépend fortement des importations extérieures, « pompées » par les bivalves cultivés qui sont ainsi directement responsables d'une forte sédimentation sous les structures mytilicoles et ostréicoles. C'est une zone de transformation.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2005/these-2260.pdf
http://archimer.ifremer.fr/doc/00000/2260/

Éditeur(s) : Elsevier
Résumé : Regardless of the degree of closure of a recirculation system, effluents are produced and replacement water is needed, which limits the possibility of locating a seawater production system away from the shoreline. At the Palavas Ifremer station, in the south of France, a High Rate Algal Pond (HRAP) was operated during several years to treat the effluent from a recirculating aquaculture system before reusing it. The effect of the HRAP-treated water on the recirculation system and on the fish was investigated and the optimal algae growing conditions were defined. The experiments were carried out in three rearing systems: one flow through, one recirculating and one recirculating with a HRAP. The water flow rate, temperature, pH and salinity conditions were similar in all systems. The effect of reusing the HRAP-treated water is very limited (1) on the functioning of the recirculation system and (2) on fish performance, but it allows a significant reduction of the dissolved inorganic nitrogen and phosphorus concentration in the rearing water. HRAP treatment reduced metal accumulation in muscle and liver of RAS fish, except for chromium and arsenic. All biomarkers presented no significant difference between systems, except for Superoxide Dismutase (SOD) and EROD, which showed a higher concentration in RAS and in both recirculating system respectively. (c) 2005 Elsevier B.V. All rights reserved.
Aquaculture (0044-8486) (Elsevier), 2006-03 , Vol. 252 , N. 1 , P. 92-101

Droits : 2005 Elsevier
http://archimer.ifremer.fr/doc/2006/publication-2306.pdf
DOI:10.1016/j.aquaculture.2005.11.053
http://archimer.ifremer.fr/doc/00000/2306/

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
BACKGROUND: Estrogen receptors (ER) α and β are found in both women and men in many tissues, where they have different functions, including having roles in cell proliferation and differentiation of the reproductive tract. In addition to estradiol (E2), a natural hormone, numerous compounds are able to bind ERs and modulate their activities. Among these compounds, phytoestrogens such as isoflavones, which are found in plants, are promising therapeutics for several pathologies. Glyceollins are second metabolites of isoflavones that are mainly produced in soybean in response to an elicitor. They have potentially therapeutic actions in breast cancer by reducing the proliferation of cancer cells. However, the molecular mechanisms driving these effects remain elusive. METHODS: First, to determine the proliferative or anti-proliferative effects of glyceollins, in vivo and in vitro approaches were used. The length of epithelial duct in mammary gland as well as uterotrophy after treatment by E2 and glyceollins and their effect on proliferation of different breast cell line were assessed. Secondly, the ability of glyceollin to activate ER was assessed by luciferase assay. Finally, to unravel molecular mechanisms involved by glyceollins, transcriptomic analysis was performed on MCF-7 breast cancer cells. RESULTS: In this study, we show that synthetic versions of glyceollin I and II exert anti-proliferative effects in vivo in mouse mammary glands and in vitro in different ER-positive and ER-negative breast cell lines. Using transcriptomic analysis, we produce for the first time an integrated view of gene regulation in response to glyceollins and reveal that these phytochemicals act through at least two major pathways. One pathway involving FOXM1 and ERα is directly linked to proliferation. The other involves the HIF family and reveals that stress is a potential factor in the anti-proliferative effects of glyceollins due to its role in increasing the expression of REDD1, an mTORC1 inhibitor. CONCLUSION: Overall, our study clearly shows that glyceollins exert anti-proliferative effects by reducing the expression of genes encoding cell cycle and mitosis-associated factors and biomarkers overexpressed in cancers and by increasing the expression of growth arrest-related genes. These results reinforce the therapeutic potential of glyceollins for breast cancer.
ISSN: 1478-811X

hal-01560288
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560288
DOI : 10.1186/s12964-017-0182-1
PUBMED : 28666461

Éditeur(s) : HAL CCSD
Résumé : International audience
Objective Macrophages may play a significant role in the initiating stages leading to chronic inflammation/infection in cystic fibrosis (CF), via an inability to act as a suppressor cell. In a previous study demonstrating impaired function of CF macrophage, we have shown high production of soluble CD14 (sCD14). The aim of the present work is to determine origin and function of sCD14 in blood monocyte-derived macrophages from stable adult CF patients. Methods To study origin, sCD14 is measured by ELISA in supernatant of macrophages from healthy subjects (non-CF) treated with PI-PLC or cholesterol. The involvement of sCD14 in inflammation, was investigated by analysis of NF-kB and caspase 1 pathways in non-CF macrophages treated by sCD14. Measurement of gene expression was done by qPCR and proteins were quantified by ELISA and Western blot. Results Treatment with PI-PLC increased sCD14 level in the supernatant of non-CF macrophages whereas cholesterol did not modified it. Furthermore high secretion of sCD14 from CF macrophages was decreased by an inhibitor of PI-PLC. Treatment with sCD14 increased pro-IL-1β, IL-8 and TNF-α expression resulting from NF-kB activation through IkBa phosphorylation, this effect was inhibited by NF-kB inhibitors. Also, activation of caspase 1 by cleavage of pro-caspase 1 allowed the secretion of mature IL-1β, inhibited by an inhibitor of caspase 1. However, inflammasome complex involved in caspase 1 activation remains to be determined. Conclusion This data show that origin of sCD14 is partly due to the action of lipases cutting the GPI tail that serves as a membrane anchor to CD14. Furthermore sCD14 have a role in chronic inflammation in CF
the 38th European Cystic Fibrosis Conference
Vienne, Austria

hal-01162773
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01162773
DOI : 10.1016/S1569-1993(15)30203-4

Résumé : After a period of rapid development of the sites (an increase in both reared surface area and production), tropical shrimp aquaculture is currently being faced with critical problems due to economical and ecological constraints. In many countries with favourable conditions for shrimp aquaculture such as indonesia or Vietnam, sites are often badly selected and/or over-exploited. This conducts to sorne extent to decreases or collapses in the production due to the difficulty in predicting the maximum production capacity of the sites. Furthermore, it appears that production sustainability depends on many factors among which socio-economy and ecolo gy are of prime importance. The STD3 project titled « Assessment of tropical shrimp aquaculture impact on the environment in tropical countries using hydrobiology, ecology and remote sensing as helping tools for diagnosis )) was initiated in 1994, with the aim to study the sustainability of marine shrimp aquaculture in tropical areas, main! y Indonesia and Vietnam, through the following tasks: (i) improve site selection and study the impact of aquaculture on marine environments presenting a variable sensitivity to organic sewage, (ii) analyse the socio-economical aspects and profitability of the aquaculture industry and of common resources (iii) use remote sensing and geographical data bases for diagnosis and monitoring of site degradation (iv) reinforce the capacity of Asian scientists through training. Severa! aquaculture sites have been investigated in the Lampung region (South Sumatra, Indonesia), the Mekong delta (Vietnam) and on the West coast of New-Caledonia, providing a large range of typical ecosystems encountered in Asia and in the Pacifie, from coralline sites to coastal plains with mangrove and deltaic areas. The study concerned the spatial structure and seasonal influence of these ecosystems, the temporal variation during the course of the project (3 years) and the relation between the ecological structure and farm productions. Remote sensing provides a synoptic vision over large land expanses. Severa! scenes were processed for landuse mapping using conventional classification techniques. Concerning water quality assessment, a general relation for the Java sea was found between image and field data in terms of total suspended matter. Applying this relation to a new site in Sumatra has provided an initial approach to water type and, together with land use mapping, a preliminary assessment of the suitability of the area to shrimp aquaculture development. Socio-economic research has been focused on the identification ofneeds for collective action, including public policy, in the perspective of shrimp farming sustainability. The main concern is in the regulation of shrimp farming intensification and extensification at the scale of coastal ecosystemic entities in a common property resource management perspective. The common considered here is coastal water quality. Comparing the local development profiles and the institutional grounds for the design and implementation of collective management rules shows that variables such as land tenure system, farm owner socio-economic profile, social homogeneity or heterogeneity of the farmers are key factors to analyse the potential for sustainability. The defmition of water quality and the possible means to ensure its collective management are discussed. The main conclusion is that the economie incentives to farming development are strong but there is no significant difference among traditional, semi-intensive and intensive systems in terms of economie efficiency or wealth distribution. The description of the functioning of the different kinds of ecosystems make it possible to give an advice concerning the positioning of the activity inside the ecosystem, and furthermore to have a reference state in order to determine the impact of the activity on the coastal environnement. This impact can be summarized as an increase in the concentration of total suspended matt€?r, of particulate organic matter and of sulfate reducing bacterias. This organic ma~.er is either issued from rearing activities or from telluric origine, due to mangrove eradication when building the ponds. An evaluation of the relationship existing between productivity and characteristics of each ecosystem bas been conducted by correlating ecological indicators data (concentration of total suspended matter, particulate organic matter, chlorophyll, percentage of pheopigments in total pigments and cyanobacteria and sulfatereducing bacteria in water and sediments) with the average production of the farms. This comparison lead to the compilation of a scale of observed production integrating the range of these environmental parameters with shrimp production levels, bound to facilitate the positioning of shrimp farming in relation with the confmement leve! of each ecosystem. Lastly, ali geographie data originated from the various compartments of the study have been geo-referenced and loaded into a geographical information system. This allows to display any query made on spatial variables and their related statistical data, including the ir variations over the last few years and to reveal patterns and phenomena otherwise not obvious.
Le programme mis en oeuvre par la DRV (Paris, CREMA-L'Houmeau, DELIAO Brest, COP Tahiti, GIE/RA Nouvelle Calédonie), dans le cadre d'un projet européen STD3 «(Sciences et Technique au Service du Développement», en collaboration avec l'Université de Montpellier II, l'Ecole Nationale d'Agronomie de Rennes, l'ITC (Hollande), l'Institut Océanographique de Nha Trang (Vietnam), le BADC de Jepara et le BPPT de Jakarta (Indonésie), avait pour but la mise au point de méthodes permettant d'améliorer la sélection des sites ainsi que le suivi du développement et de l'impact de l'aquaculture des crevettes Péneides en milieu tropical pour éviter la surexploitation. Quatre axes de recherche ont été défmis : a) identifier la nature et quantifier les rejets de déchets issus de ce type d'aquaculture, b) définir les caractéristiques écologiques des écosystèmes susceptibles d'accueillir l'activité aquacole et suivre leur évolution sous l'influence des rejets, c) déterminer l'influence de la gestion des fermes et des ressources (aspects économiques) sur les performances de productivité, d) déterminer la capacité de la télédétection comme outil de diagnostic d'impact à l'échelle de la région, e) mener un programme de formation d'étudiants et jeunes chercheurs Vietnamiens et Indonésiens.

Droits : 1998 Ifremer
http://archimer.ifremer.fr/doc/00132/24357/22357.pdf
http://archimer.ifremer.fr/doc/00132/24357/

Éditeur(s) : HAL CCSD
Résumé : The mangroves play a vital role in the ecological balance. They provide marine ecosystem resources and are involved in its stabilization and in the coastal protection. They constitute the growth areas of many species that feed marine species and particularly shrimp. We develop a standard fishery model by integrating mangroves area into the analysis. The extended model shows that mangrove plays animportant role in shrimp production and data related to it are well supported by the model. We analyze the e¤ects of mangrove on open access of the French Guiana shrimp fishery and show that mangrove is not the only factor that plays a vital role in the French Guiana shrimp fishery.
https://hal.univ-antilles.fr/hal-01383024

hal-01383024
https://hal.univ-antilles.fr/hal-01383024
https://hal.univ-antilles.fr/hal-01383024v2/document
https://hal.univ-antilles.fr/hal-01383024/file/Diop%20et%20al.%20%282016%29.pdf

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
The actin cytoskeleton is a dynamic structure that provides an interactive platform for organelles and cellular components. It also serves as track for membranes and vesicles that move via myosin. The actin cytoskeleton of Symbiodinium is a well-organized reticular structure suggestive of multiple membrane interactions, very likely including those of the chloroplast. The Symbiodinium chloroplast membrane network is, in turn, a highly organized structure, suggestive of being under the control of an organizing network. We visualized the chloroplast membranes of cultured Symbiodinium sp. under various light conditions and observed changes dependent on illumination intensity. Since we suspected interaction between these two organelles, and we knew that the Symbiodinium actin cytoskeleton collapses upon treatment with either latrunculin B, an actin microfilament-disrupting agent, or butanedione monoxime, a myosin function inhibitor, we tested the Symbiodinium sp. oxygen evolution in their presence. Upon latrunculin B addition, the oxygen production decreased compared to non-treated cells; however, this was not observed after a 24 h latrunculin treatment. On the contrary, butanedione monoxime treatment caused a non-recoverable dysfunction of the chloroplast causing a severe loss in oxygen production even after long-term exposure. Using electron microscopy, we observed an alteration of the Symbiodinium sp. chloroplast distribution after latrunculin B treatment, with respect to untreated cells. Furthermore, a thorough disorganization of the chloroplast grana was observed after butanedione monoxime treatment. These data suggest that an actomyosin system would be important for chloroplast organization and distribution, and critical for normal photosynthetic function of Symbiodinium sp.
ISSN: 0166-8595

hal-01546164
https://hal.archives-ouvertes.fr/hal-01546164
DOI : 10.1007/s11120-015-0142-9