DEDUCTION : A research project for shrimp farming sustainability in New-Caledonia Auteur(s) : Beliaeff, Benoit Chim, Liet Della Patrona, Luc Goyard, Emmanuel Herlin, Jose Labreuche, Yannick Walling, Emilie Ansquer, Dominique Éditeur(s) : The 11th Pacific Science Inter-Congress : Pacific Countries and their Ocean: Facing Local and Global Changes. March 2 - 6, 2009 Tahiti, French Polynesia Résumé : New Caledonian shrimp farming feasibility studies started in the mid-seventies. Today ca. 2000 tons of Litopenaeus stylirostris, introduced in 1978, are semi-intensively produced each year. Since fifteen years, production has been impacted by two seasonal vibriosis, the “Summer” syndrome caused by Vibrio nigripulchritudo in relation to pond bottom and water column quality, and the “Winter Syndrome” caused by Vibrio penaeicida in relation to drastic temperature drops . With the ambition of elucidating processes possibly leading to mortality outbreaks in ponds, Ifremer has been conducting a research program since 2003 under the DESANS project (2003-2006) and the present DEDUCTION project, equally funded by the South and North Provinces and by the Government of New Caledonia. This program has been designed in a multidisciplinary approach integrating the suspected multifactorial origin of shrimp mortalities. Environment quality in ponds (either sediment or water column) plays a major role in the shrimp ecophysiological status, influencing sensitivity to pathogens . DEDUCTION focuses on a better knowledge of phytoplankton composition and biogeochemical fluxes at the sediment-water interface in ponds. Impact of farm discharges is also investigated. In addition, a more fundamental work is conducted to understand the pathogenicity mechanisms of these two Vibrio species but also to prevent the occurrence of viral infections through a RNA interference approach. Markers of the ecophysiological status of broodstock, larvae, post-larvae and reared animals, such as enzymes testifying from an oxidative stress, are developed. Gain of performance (resistance to pathogens and growth) were obtained on hybrids of the New Caledonian and introduced Hawaiian strains. Finally the database Stylog is exploited in view of discriminating “good” from “bad” rearings on the basis of relevant indicators. Les études de faisabilité de l'aquaculture de crevettes ont débuté en Nouvelle-Calédonie dans les années soixante dix. Aujourd'hui environ 2000 tonnes de Litopenaeus stylirostris, introduite en 1978, sont produites chaque année de façon semi-intensive. Depuis quinze ans la production a été impactée par deux vibrioses : le syndrome d'été provoqué par Vibrio nigripulchritudo en relation avec la qualité de la tranche d'eau et des fonds de bassins et le syndrome d'hiver provoqué par Vibrio penaeicida en relation avec des chutes rapides de température. Dans le but d'élucider les processus entrainant des mortalités dans les élevages, Ifremer a conduit un programme de recherche depuis 2003 dans le cadre du projet DESANS (2003-2006) puis du présent projet DEDUCTION co-financé par les provinces Nord et Sud et le Gouvernement de la Nouvelle-Calédonie. Ce programme se construit dans une démarche multidisciplinaire intégrant les origines supposées multifactorielles des mortalités de crevettes. La qualité environnementale dans les bassins (les fonds et la colonne d'eau) joue un rôle majeur dans l'état physiologique des animaux, influençant leur sensibilité aux pathogènes. DEDUCTION se focalise sur une meilleure compréhension de la composition phytoplanctonique et des flux bio-géochimiques à l'interface eau/sédiment dans les bassins.L'impact des rejets des fermes est également étudié. En outre, un travail plus fondamental est conduit pour connaître les mécanismes de pathogénicité de ces deux espèces de Vibrio mais aussi pour prévenir les occurences d'infections virales par des approches ARN Interférence. Des marqueurs de l'état physiologique des oeufs, larves, post-larves et animaux en élevage, tels que les enzymes du stress oxydant sont utilisés.Les gains de performance (résistance aux pathogènes et croissance) sont obtenus sur des hybrides issus du croisement de la souche locale avec une souche hawaienne. Finalement la base de données STYLOG est exploitée dans le but de distinguer les bonnes des mauvaises pratiques sur la base d'indicateurs fiables. Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/00143/25438/23591.pdf http://archimer.ifremer.fr/doc/00143/25438/ | Partager |
Eutrophication in a tropical pond: Understanding the bacterioplankton and phytoplankton dynamics during a vibriosis outbreak using flow cytometric analyses Auteur(s) : Lucas, Ronan Courties, C. Herbland, Alain Goulletquer, Philippe Marteau, Anne-laure Lemonnier, Hugues Éditeur(s) : Elsevier Science Bv Résumé : In tropical shrimp ponds, the increasing of feed input, concomitantly with the stocking shrimp biomass, induces an eutrophication of the ecosystem. Although difficult to maintain, its stability is required to guarantee the success of the culture. A 110-day period of phytoplankton and bacterioplankton stock and dynamics in an earthen pond (1.2 ha area, 1 m depth) was monitored using flow cytometry to provide baseline information on community characteristics and ecosystem instability. Seven autotrophic cell types were identified over the whole sampling period. Prokaryotic cells included Synechococcus sp., a group named UNK which presented an atypical new flow cytometric signature and picoeukaryotes (PEUK). Nanophytoplankton cells were represented by 4 groups: NAN1, NAN2, NAN3 and Cryptophytes. During the first part of the survey, picophytoplankton dominated the phytoplanktonic assemblage. The mean abundance of total cells (up to 8 x 10(6) cells mL(-1)) was among the highest recorded in marine and brackish waters. Bacterial abundance and production ranged from 0.8 to 5.1 x 10(7) cells mL(-1) and from 30 to 110 mu g C L-1 h(-1). A shift from pico to nanophytoplankton abundance was observed for a few days from d 96. During this period, heterotrophic bacteria production and abundance suddenly dropped, implying a change in the functioning of the microbial loop. This shift was concomitant with a significant shrimp mortality outbreak due to Vibrio penaeicida, the etiological agent of a disease known as Syndrome 93, which affects the shrimp industry in New Caledonia. This survey suggests that flow cytometric analysis could be used for the monitoring of aquaculture systems to improve our understanding of the complex phytoplankton and bacterial dynamics of these systems and its potential influence on disease development. (c) 2010 Elsevier B.V. All rights reserved. Aquaculture (0044-8486) (Elsevier Science Bv), 2010-12 , Vol. 310 , N. 1-2 , P. 112-121 Droits : 2010 Elsevier B.V. All rights reserved. http://archimer.ifremer.fr/doc/00029/14068/11312.pdf DOI:10.1016/j.aquaculture.2010.10.022 http://archimer.ifremer.fr/doc/00029/14068/ | Partager Voir aussi Tropical shrimp aquaculture Ecosystem shift Phytoplankton Bacterioplankton Vibrio Flow cytometry Télécharger |
Effluents from land based marine farms: nature, treatment, valorisation, modelisation. Applications to fish and shrimp rearing Auteur(s) : Hussenot, Jerome Lefebvre, Sebastien Martin, Jean-louis Éditeur(s) : Man and Coastal Areas – Towards a sustainable Aquaculture, May 2000 Résumé : Fish culture (seabass, turbot...) on the French metropolitan coast, and shrimp culture in the overseas territories (essentially New Caledonia and Tahiti) are subject to a regular development through land based farms. Three thematic of research on aquaculture ponds are carried out in CREMA-L’Houmeau, in collaboration with producers. The aim of the first one is to determine the optimal rearing conditions (stocking density, water renewal rate, input of oxygen, rate of feeding...) which allow to minimise the quantity of wastes for a similar final biomass. The second part aims to reduce the nutrient loading in the effluent, through open-air treatments: sedimentation ponds, foam fractionation, mass production of phytoplankton, and rearing of molluscs on the produced phytoplankton. The third part concerns research aiming to model the “ production - treatment - valorisation ” relationships. Droits : 2000 Ifremer http://archimer.ifremer.fr/doc/00016/12728/9650.pdf http://archimer.ifremer.fr/doc/00016/12728/ | Partager |
Quantification of Vibrio penaeicida, the etiological agent of Syndrome 93 in New Caledonian shrimp, by real-time PCR using SYBR Green I chemistry Auteur(s) : Goarant, Cyrille Merien, F Éditeur(s) : Elsevier Résumé : Shrimp farming is a small but growing industry in New Caledonia. Since 1993, "Syndrome 93" has been affecting New Caledonian shrimp farming industry every cold season, causing severe epizootic mortalities in grow-out ponds and significant losses. Highly pathogenic strains of Vibrio penaeicida are considered the etiological agent of the disease in Litopenaeus stylirostris. On one hand, studies demonstrated that healthy shrimp may carry V penaeicida for weeks with a high overall prevalence, regardless of any seasonal pattern or temperature conditions. On the other hand, larvae are free of V penaeicida and are also resistant to experimental infection. V penaeicida is frequently detected in incoming water pumped from the bays, which was shown, by a molecular typing study, to be the infectious source. This particular epidemiological pattern highlights the major role of the factors that trigger and aggravate the disease in grow-out ponds, where shrimp populations carry the pathogen all year round. In order to gain a better understanding of "Syndrome 93" epidemiology, quantification of V penaeicida both in shrimp and the shrimp farm ecosystem is necessary. This article describes the steps in the successful development of a real-time PCR quantification assay of V penaeicida in shrimp haemolymph, seawater (from ponds or bays) and sediment pore water, including the choice of an accurate extraction technique. The entire detection method; including sample processing, DNA extraction and real-time PCR amplification, can be completed within 4 h. (c) 2006 Elsevier B.V All rights reserved. Journal of Microbiological Methods (0167-7012) (Elsevier), 2006-10 , Vol. 67 , N. 1 , P. 27-35 Droits : 2006 Elsevier http://archimer.ifremer.fr/doc/2006/publication-1903.pdf DOI:10.1016/j.mimet.2006.02.013 http://archimer.ifremer.fr/doc/00000/1903/ | Partager |
Effect of water exchange on effluent and sediment characteristics and on partial nitrogen budget in semi-intensive shrimp ponds in New Caledonia Auteur(s) : Lemonnier, Hugues Faninoz, Sebastien Éditeur(s) : Blackwell science Résumé : An experiment was conducted in six earthen ponds with 20 shrimps m(-2) Litopenaeus stylirostris (Stimpson) during the warm season in New Caledonia to study the dynamics of wastes in relation with water exchange rate (WER). The nitrogen budget was established, taking into account the different forms of nitrogen in the water, sediment, feed and shrimp. Data from a wide range of treatments applied in unreplicated ponds were treated using regression analysis to establish the relationship between WER and partial nitrogen budget, sediment characteristics and shrimp performance. To compare effluent quality between treatments during the season, data were analysed using the non-parametric sign test. The water outflow was characterized by a decrease in the concentrations of N-mineral forms (TAN, NO2--NO3-), an increase in the concentration of organic soluble and sestonic organic forms (expressed in terms of particulate nitrogen, particulate organic carbon, chlorophyll a) compared with the water inflow. Increasing WER increased the amount of exported wastes and mainly in the organic forms and TAN can be considered as negligible. The nitrogen budget showed that 19-46% of nitrogen input (feed+water) was exported into the coastal environment. The results showed that the quality of the sediment decreased as WER decreased. The potential negative impact of the developing industry in New Caledonia on the costal environment could be partially reduced in a first step by decreasing WER. However, if applied in the farms, this practice should be linked to a close survey of the evolution of sediment quality. Aquaculture Research (1355-557X) (Blackwell science), 2006-06 , Vol. 37 , N. 9 , P. 938-948 Droits : 2006 Blackwell Publishing, Inc. http://archimer.ifremer.fr/doc/2006/publication-1811.pdf DOI:10.1111/j.1365-2109.2006.01515.x http://archimer.ifremer.fr/doc/00000/1811/ | Partager |
Sequence polymorphism-based identification and quantification of Vibrio nigripulchritudo at the species and subspecies level targeting an emerging pathogen for cultured shrimp in New Caledonia Auteur(s) : Goarant, Cyrille Reynaud, Yann Ansquer, Dominique De Decker, Sophie Merien, Fabrice Éditeur(s) : Elsevier Résumé : In a previous study, we demonstrated the existence of an emerging cluster of Vibrio nigripulchritudo that proved to be associated with shrimp mortality events in New Caledonia. Using sequence polymorphisms evidenced in this previous MultiLocus Sequence Typing study, we developed two new quantitative PCR assays permitting the detection and quantification of V. nigripulchritudo at the genospecies level using SYBR Green I chemistry and at the emerging cluster level using Fluorescence Resonance Energy Transfer technology with hybridization probes. The use of this molecular diagnostic tool evidenced the colonization of the shrimp pond ecosystem by the pathogenic cluster at least at the onset of the disease. This new tool will allow better investigation of the dynamics of this bacterial pathogen in the shrimp farm ecosystem. (C) 2007 Elsevier B.V. All rights reserved. Journal of Microbiological Methods (0167-7012) (Elsevier), 2007-07 , Vol. 70 , N. 1 , P. 30-38 Droits : 2007 Elsevier B.V. All rights reserved http://archimer.ifremer.fr/doc/2007/publication-2729.pdf DOI:10.1016/j.mimet.2007.03.007 http://archimer.ifremer.fr/doc/00000/2729/ | Partager |
Influence of sediment characteristics on shrimp physiology: pH as principal effect Auteur(s) : Lemonnier, Hugues Bernard, E Boglio, E Goarant, Cyrille Cochard, Jean-claude Éditeur(s) : Elsevier Résumé : Penaeid shrimp reared in earthen ponds are exposed to sediment, which can, in some instances, induce a stress. In seawater, the osmoregulatory capacity (hyporegulation) is a useful tool to compare the physiological condition of shrimp exposed to various stressors. By keeping some shrimp in cages at different locations of a single pond heterogeneous in terms of sediment quality, it was possible, using osmotic pressure (OP), to identify some locations where the stress was maximum and some others where it was minimum. Simultaneously, sediment samples were taken and analysed in order to evaluate some physico-chemical parameters that could be related to the stress observed in the shrimps kept in the cages. This approach allowed to show a significant positive correlation between the pH of the sediment surface and the shrimp osmotic pressure. This result was confirmed in a study carried out in experimental 70-1 tanks, where osmotic pressure decreased significantly as water pH decreased from 7.0 to 6.5. The methodology developed in this study may be useful to evaluate the stress caused by sediment in shrimp farms. (C) 2004 Elsevier B.V. All rights reserved. Aquaculture (0044-8486) (Elsevier), 2004-10 , Vol. 240 , N. 1-4 , P. 297-312 Droits : 2004 Elsevier http://archimer.ifremer.fr/doc/2004/publication-1866.pdf DOI:10.1016/j.aquaculture.2004.07.001 http://archimer.ifremer.fr/doc/00000/1866/ | Partager |
Metal and metalloid bioaccumulation in the Pacific blue shrimp Litopenaeus stylirostris (Stimpson) from New Caledonia: Laboratory and field studies Auteur(s) : Metian, Marc Hedouin, Laetitia Eltayeb, Mohamed M. Lacoue-labarthe, Thomas Teyssie, Jean-louis Mugnier, Chantal Bustamante, Paco Warnau, Michel Éditeur(s) : Pergamon-elsevier Science Ltd Résumé : The present work aimed at better understanding metal and metalloid bioaccumulation in the edible Pacific blue shrimp Litopenaeus stylirostris, using both laboratory and field approaches. In the laboratory, the bioaccumulation kinetics of Ag, Cd, Co, Cr, and Zn have been investigated in shrimp exposed via seawater and food, using the corresponding gamma-emitting radiotracers (Ag-110m, Cd-109, Co-57, Cr-51, and Zn-65) and highly sensitive nuclear detection techniques. Results showed that hepatopancreas and intestine concentrated the metals to the highest extent among the blue shrimp organs and tissues. Moulting was found to play a non negligible detoxification role for Co, Cr and, to a lesser extent, Zn. Metal retention by L stylirostris widely varied (from a few days to several months), according to the element and exposure pathway considered (a given metal was usually less strongly retained when ingested with food than when it was taken up from the dissolved phase). In the field study, Ag, As, Cd, Co, Cr, Cu, Mn, Ni, and Zn were analysed in shrimp collected from a New Caledonian aquaculture pond. Metal concentrations in the shrimp muscles were generally relatively low and results confirmed the role played by the digestive organs and tissues in the bioaccumulation/storage/detoxification of metals in the Pacific blue shrimp. Preliminary risk considerations indicate that consumption of the shrimp farmed in New Caledonia is not of particular concern for human health. (C) 2010 Elsevier Ltd. All rights reserved. Marine Pollution Bulletin (0025-326X) (Pergamon-elsevier Science Ltd), 2010 , Vol. 61 , N. 7-12 , P. 576-584 Droits : 2010 Elsevier Ltd All rights reserved. http://archimer.ifremer.fr/doc/00014/12528/9869.pdf DOI:10.1016/j.marpolbul.2010.06.035 http://archimer.ifremer.fr/doc/00014/12528/ | Partager |
Response of the blue shrimp Litopenaeus stylirostris to temperature decrease and hypoxia in relation to molt stage Auteur(s) : Mugnier, Chantal Soyez, Claude Éditeur(s) : Elsevier Résumé : The effect of different levels of dissolved oxygen (DO) and a decreased temperature combined with severe hypoxia were studied in the blue shrimp Litopenaeus stylirostris in relation to molt stage. In the first experiment, shrimps were submitted to DO concentrations of 5 to 1 mg l(-1). Osmoregulatory capacity (OC) was measured in shrimps at molt stage C after 6, 24, or 48 h of exposure. No mortality was recorded but a significant negative effect of DO concentration on hypo-OC was observed for DO concentrations below 3 mg l(-1). Osmoregulation improved after 48 h exposure, compared to 6 and 24 h exposure. In a second experiment, temperature was decreased from 28 to 22 degreesC in 24 h, before a severe hypoxia of 0.5+/-0.1 mg O-2 l(-1) was applied. Mortality and OC were studied in relation to molt stages. Half of the shrimps died after respectively 100 min at 0.5 mg O-2 l(-1) at 28 degreesC and 153 min at 0.4 mg O-2 l(-1) at 22degreesC. Mortality was significantly higher in stages D-2 and B compared to stages C and Do. Control shrimps in stage D2 had a lower hypo-OC than shrimps in stages C and Do both at 28 and 22 degreesC. Temperature decrease had no effect on hypo-OC. Hypoxia reduced hypo-OC for all stages, whatever the temperature, but the effect was greater in stage C at 27 degreesC (68% decrease) compared to 22 degreesC (49%) and stage D-2 at both temperatures (respectively, 43% and 58% at 27 and 22 degreesC). Combination of temperature and hypoxia had a significant effect on hypo-OC. Low temperature reduced the effect of hypoxia presumably by slowing down the metabolism. Results are discussed in relation to pond observation. (C) 2004 Elsevier B.V. All rights reserved. Aquaculture (0044-8486) (Elsevier), 2005-02 , Vol. 244 , N. 1-4 , P. 315-322 Droits : 2004 Elsevier B.V. All rights reserved. http://archimer.ifremer.fr/doc/2005/publication-372.pdf DOI:10.1016/j.aquaculture.2004.11.010 http://archimer.ifremer.fr/doc/00000/372/ | Partager Voir aussi Temperature Stress Penaeid shrimp Osmoregulation Molt stage Litopenaeus stylirostris Hypoxia Télécharger |
Les sols des fonds de bassins et leur gestion durant les assecs. Etat des connaissances Auteur(s) : Della Patrona, Luc Brun, Pierre Herbland, Alain Résumé : Shrimp culture in New Caledonia has developed according to the "intensified" semi-intensive model which uses ponds that stretch over several hectares. Local shrimp farms are for the most part installed in "tannes", barren, highly saline areas of the back mangrove forest. These areas generally have impermeable silty clay soils that have biogeochemical features particularly appropriate for benthic life and are often immersed by tides. During the six months of the grow-out phase, the "pond ecosystem" undergoes an increase in potential, progressing from an initial state of "natural lagoon" to a "grow-out pond" that is highly enriched with degrading organic matter.... La crevetticulture calédonienne s'est développée sur le modèle semi intensif « intensifié » qui se pratique en bassins de plusieurs hectares. Les fermes aquacoles locales sont pour la majorité implantées sur des « tannes », zones salées et nues d'arrière mangrove. Ces étendues présentent des sols généralement limono-argileux imperméables dont les caractéristiques bio géochimiques sont le plus souvent appropriées à la vie benthique car fréquemment recouvertes par la marée. Au cours des six mois d'élevage, on assiste à une montée en puissance de l' « écosystème-bassin » qui passe d'un état initial de « lagune naturelle » à celui final d'un « bac d'élevage très enrichi » en produits de dégradation de la matière organique. Le premier « fauteur de troubles » dans un bassin est l'aliment, ou plutôt les conditions imparfaites de l'alimentation qui contribuent fortement à la détérioration de la qualité de la colonne d'eau et du sol et conduisent à des accumulations. Avec la succession de cycles « forcés » par souci de rentabilité, le bassin peut devenir extrêmement consommateur d'oxygène et des zones de plus en plus vastes se recouvrent de vases noires susceptibles de libérer des composés réduits toxiques limitant l'espace vie de la crevette. La dégradation des fonds de bassin n'est cependant pas une fatalité. Les boues bien gérées sont un atout pour le fonctionnement harmonieux d'un tel agro-système et en particulier pour le bien être du cheptel. Le maintien d'une qualité favorable de sédiment pour la production de L.stylirostris passe par la mise en assec d'une durée minimale d'au moins deux semaines. Cette période inter élevage de réhabilitation « à l'air » sera d'autant plus courte et efficace que la zootechnie « en eau » aura été soignée. La Demande en Oxygène du Sédiment (DOS), les Matières Aisément oxydables (MAO), le Redox, l'abondance de la méiofaune, le rapport Protéines/Glucides de la Matière Organique peuvent renseigner les aquaculteurs sur la qualité de leur « foncier ». Ces paramètres prometteurs sont cependant difficiles à mettre en oeuvre au sein des entreprises. Ils sont abordés ici dans le cadre d'un rappel didactique de certaines bases biologiques essentielles pour tirer le meilleur parti de l'assec. Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/2007/rapport-4399.pdf http://archimer.ifremer.fr/doc/00000/4399/ | Partager |
Amélioration génétique expérimentale de la crevette d'élevage de Nouvelle-Calédonie : Sélection d'une population de L. stylirostris résistante à la bactérie pathogène Vibrio penaeicida. Rapport final pour le Ministère de l'Outre-Mer Auteur(s) : Goyard, Emmanuel Goarant, Cyrille Bachere, Evelyne De Lorgeril, Julien Mugnier, Chantal Ansquer, Dominique Broutoi, Francis Brun, Pierre Résumé : The New-Caledonian shrimp industry is based on the controlled reproduction of the shrimp Litopenaeus stylirostris, a species which was introduced in the 80s. The major difficulty to which the industry has been faced for 10 years is the occurrence of the "syndrome 93", which corresponds to mortality phases when the temperature falls down in April-May-June. This mortality is associated to the pathogenic bacteria Vibrio penaecida and is expressed at different levels which are variable from year ta year and from pond to pond. No resistance to this pathology has been developed spontaneously. This is likely due to the protocole used to rear spawners, which does not allow to implement an efficient selective pressure at each generation
An experimental selection on the criteria of survival after picks of syndrome 93 has been conducted at the Laboratoire Aquacole de Calédonie. The 3rd selected generation demonstrates survival rates improved by 20% during experimental infections with V. penaeicida in comparison with a non selected control population of same genetic origin. The comparison of the correlated responses on the level of expression of 5 genes which are potentially implicated in immunity phenomena (Peneidins, lysozyme, transglutaminase. profiline, annexine) shows that the selected population has a level of expression in lyzozyme twice higher than the control population. This result suggests that the lysozyme could be a genetic marker which could be used in a selective breeding program to he developed in relation with the private hatcheries. La filière crevette de Nouvelle-Calédonie repose sur la maîtrise de la reproduction contrôlée de la crevette Litopenaeus stylirostris, espèce introduite dans les années 80. La difficulté majeure que rencontre la filière depuis une dizaine d'années est la récurrence du « syndrome 93 », qui correspond à des épisodes de mortalités lors des baisses de température en avril-mai-juin. Ces mortalités sont associées à la bactérie pathogène Vibrio penaeicida et s'expriment à des niveaux d'intensité variable d'une année à l'autre et d'un bassin à l'autre. Aucune résistance vis-à-vis de cette pathologie ne s'est développée spontanément. Ceci est vraisemblablement lié au protocole employé pour l'élevage des géniteurs qui ne permet pas d'exercer une pression de sélection efficace à chaque génération. Une expérience de sélection sur un critère de survie à des épisodes de syndrome 93 a été menée au Laboratoire Aquacole de Calédonie. La 3ème génération sélectionnée montre des survies améliorées de l'ordre de 20% lors d'infections expérimentales à V. penaeicida par rapport à une population témoin non sélectionnée de même origine génétique. La comparaison des réponses corrélées sur le niveau d'expression de 5 gènes potentiellement impliqués dans les phénomènes de défense immunitaire (penaeidine, lysozyme, transglutaminase, profiline, annexine) montre que la population sélectionnée a un niveau d'expression en lysozyme deux fois plus élevé que la population témoin. Ce résultat suggère que le lysozyme pourrait être un marqueur génétique utilisable dans un programme de sélection à développer en relation avec les écloseries de production. Droits : 2003 Ifremer http://archimer.ifremer.fr/doc/00109/22020/19643.pdf http://archimer.ifremer.fr/doc/00109/22020/ | Partager |
Biofloc technology applied to rear shrimp Litopenaeus Stylirostris broodstock: An integrated and development research project in New Caledonia and French Polynesia Auteur(s) : Chim, Liet Cardona, Emilie Lorgeoux, Benedicte Gueguen, Yannick Saulnier, Denis Goguenheim, Jean Wantiez, Laurent Cahu, Chantal Éditeur(s) : Europe Aqauculture Symposium, October 14-17, San Sebastian, Spain Résumé : Shrimp farming in New-Caledonia faces up to difficulties. A fluctuating quality of broodstock prevents sustainable production of larvae in quantity and quality. The traditional extensive rearing method of broodstock in outdoor earthen ponds used in New Caledonia raises several issues: poor water quality control and biosecurity. Therefore it is fundamental to modify the broodstock culture strategy in order to achieve an easier water quality management and maximal biosecurity. Biofloc technology (BFT) offers easier water quality management, higher natural productivity, higher level of biosecurity, and could be a good alternative. Since 2011 a R&D program started under the supervision of IFREMER in New Caledonia and French Polynesia in order to study and develop biofloc technology for rearing shrimp L. stylirostris broodstock. In a first step we showed that biofloc (BFT) improved significantly the reproductive performances of shrimps and the quality of their larvae compared to Clear Water (CW) or Earthen Pond (EP), with: • A better final survival rate of broodstock from BFT compare to CW. • Females from BFT produced more eggs per spawn and spawned more frequently compared to females from CW or EP. • The larvae from BFT females had a significantly higher survival rate compared to larvae from CW females, respectively 70% (n=4) and 45% (n=4). In a second step we studied the underlying biological mechanisms explaining the better reproductive performance and health status of the broodstock from BFT rearing system, and the better quality of their larvae. We showed that the juvenile shrimp fed actively on biofloc, which represented 60% of their whole food (40% coming from pelleted diet). Biofloc represents a significant source of essential nutrients (vitamins, antioxidants, highly unsaturated fatty acids, …) or dietary bacteria which could act as probiotic. These dietary complements could explain the improved health status (immunity and oxidative status status), the better resistance to oxidative stress and a systematically better survival rate of the shrimp reared in BFT compared to those reared in CW. Droits : 2014 Ifremer http://archimer.ifremer.fr/doc/00250/36084/34635.pdf http://archimer.ifremer.fr/doc/00250/36084/ | Partager |
"Summer Syndrome" in Litopenaeus stylirostris in New Caledonia: Pathology and epidemiology of the etiological agent, Vibrio nigripulchritudo Auteur(s) : Goarant, Cyrille Ansquer, Dominique Herlin, Jose Domalain, David Imbert, Frederic De Decker, Sophie Éditeur(s) : Elsevier Résumé : The Summer Syndrome is a new shrimp disease that has been affecting a shrimp growout farm in New Caledonia since end of 1997. It was recognized to be caused by a systemic vibriosis due to Vibrio nigripulchritudo. This new disease turned out almost immediately enzootic in the shrimp farm involved and has affected all its crops ever since. Since the year 2000, V. nigripulchritudo strains have been found in several shrimp farms, although Summer Syndrome is still limited to one particular area, affecting, since 2003, two adjoining farms. As part of a multidisciplinary research program, a high-frequency survey was carried out during the summer 2002-2003 in two shrimp farms: one affected farm and another one in which pathogenic strains could be isolated but without any disease event. It permitted a good description of V. nigripulchritudo dynamics in shrimp and the ecosystem in both farms. The study of virulence characteristics of some isolates showed that pathogenic and non-pathogenic strains occur in the shrimp farms environment and that both may be found at the same time in one farm. Our results strongly suggest a persistence of pathogenic strains in wet zones of the pond sediment at low concentrations between crops in the affected farm, and their development during the rearing cycle as a cause of shrimp infection. Aquaculture (0044-8486) (Elsevier), 2006-03 , Vol. 253 , N. 1-4 , P. 105-113 Droits : 2005 Elsevier B.V. All rights reserved http://archimer.ifremer.fr/doc/2006/publication-1110.pdf DOI:10.1016/j.aquaculture.2005.07.031 http://archimer.ifremer.fr/doc/00000/1110/ | Partager |
A soft technology to improve survival and reproductive performance of Litopenaeus stylirostris by counterbalancing physiological disturbances associated with handling stress Auteur(s) : Wabete, Nelly Chim, Liet Pham, Dominique Lemaire, Pierrette Massabuau, J Éditeur(s) : Elsevier Résumé : The consequences of handling stress (fishing, transfer, eyestalk ablation) on shrimp broodstock are poorly documented. The weakness of farmed shrimp, Litopenaeus stylirostris, during winter is a major problem in New Caledonia, because of seasonal climate (tropical-sub-temperate). The transfer of broodstock in winter from earthen outdoor ponds to indoor maturation tanks in the hatchery (T=20 degrees C, Salinity=35 parts per thousand, fed shrimp) usually leads, after 48 h, to high mortality (up to 70%). Eyestalk ablation to induce ovarian maturation in females leads to further mortality. Starting from a background analysis of physiological disturbances (initial osmoregulatory imbalance) associated with handling stress (Wabete, N., Chim, L., Lernaire, P., Massabuau, J.-C., 2004. Caracterisation de problemes de physiologie respiratoire et d'echanges ioniques associes A la manipulation chez la crevette peneide Litopenaeus stylirostris a 20 degrees C. Styli 2003. Trente ans de crevetticulture en Nouvelle-Caledonie. Ed. Ifremer. Actes Colloq. 38, 75-84.), we developed aprotocol using a soft technology, based on modifications of water salinity, temperature and feeding regime. The aim was to minimize problems of osmoregulatory imbalance and associated mortalities. The protocol we developed, called the LSD OT protocol (Low Salinity and Diet, Optimal Temperature), was first evaluated on sub-adult shrimp (20-25 g) and then applied to broodstock. Survival after transfer and following eyestalk ablation, as well as reproductive achievement (spawning rate, nauplii number) was considerably improved when shrimps were transferred under "physiological comfort" i.e. warmed isosmotic water (26 degrees C and 26 parts per thousand) and unfed for 3 d. This new handling protocol, based on a better control of salinity, temperature and feeding conditions, has been transferred successfully to private hatcheries and already contributes to an increased profitability of New-Caledonian shrimp industry. (c) 2006 Elsevier B.V. All rights reserved. Aquaculture (0044-8486) (Elsevier), 2006-09 , Vol. 260 , N. 1-4 , P. 181-193 Droits : 2006 Elsevier http://archimer.ifremer.fr/doc/2006/publication-1880.pdf DOI:10.1016/j.aquaculture.2006.06.041 http://archimer.ifremer.fr/doc/00000/1880/ | Partager |
"Summer syndrome" in Litopenaeus stylirostris grow out ponds in New Caledonia: zootechnical and environmental factors Auteur(s) : Lemonnier, Hugues Herbland, Alain Salery, Lucas Soulard, Benoit Éditeur(s) : Elsevier Résumé : The "Summer Syndrome", a septicemic vibriosis caused by Vibrio nigripulchritudo, is responsible for one of the two main seasonal mortalities which affect shrimp aquaculture in New Caledonia. It was identified for the first time in December 1997 in an intensive shrimp farm (called farm DF) and has been enzootic ever since. The "Summer syndrome" affects shrimp during warm-season growout. Although the geographic area concerned is limited, it is a potential threat for the industry in that the disease could spread. Analysis of grow out data from 1991 to 2002 in this farm was carried out in order to characterize the disease and the environmental conditions in relation to mortality. Results of farm DF were compared to data from farms not affected by the disease (called farms HC). The "Summer syndrome" occurs during the warm season which is characterized by an increase in water temperature and sunny duration. This disease is characterized by simultaneously highest densities and weakest drying duration between crops in the farms studied. Between 1991 and 2002, the increase of feed input and nitrogen rate in feed has led to a significant increase of the growth rate over the years in farm DF. At the same time, water management has not changed and has induced an increase in environmental waste production. In consequence, an early eutrophication of water is observed, which is concomitant with the beginning of the mortality outbreaks. This could play a role by inducing directly or indirectly a stress for shrimp and/or a growth and/or virulence factors of the pathogen. Aquaculture (0044-8486) (Elsevier), 2006-08 , Vol. 261 , N. 3 , P. 1039-1047 Droits : 2006 Elsevier http://archimer.ifremer.fr/doc/2006/publication-1868.pdf DOI:10.1016/j.aquaculture.2006.08.036 http://archimer.ifremer.fr/doc/00000/1868/ | Partager |
Acquisition of susceptibility to Vibrio penaeicida in Penaeus stylirostris postlarvae and juveniles Auteur(s) : Goarant, Cyrille Regnier, France Brizard, Raphael Marteau, Anne-laure Éditeur(s) : Elsevier Résumé : Vibriosis is a major disease problem in shrimp aquaculture, affecting all developmental stages, from larvae in hatchery tanks to juveniles and broodstock in growout ponds. However, bacterial strains responsible for vibriosis in the successive stages are usually considered to be different, and virulence specificity has been reported both at the species and at the stage levels. The so-called « Syndrome 93 » is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Penaeus stylirostris in growout ponds and broodstock tanks in New Caledonia. This pathology does not cause any mortality in hatchery or nursery phases. An experimental infection design using balneation of postlarvae and early juveniles in V. penaeicida suspensions was used to evaluate the developmental stage at which shrimp become sensitive to this vibriosis. We demonstrated that the acquisition of susceptibility to this pathogen is very sudden and correlated with the acquisition of the definitive rostral formula, and from this draw conclusions regarding virulence mechanisms of V. penaeicida in P. stylirostris. Aquaculture (Elsevier), 1998-12 , Vol. 169 , N. 3-4 , P. 291-296 Droits : 1998 Elsevier Science B.V. All rights reserved http://archimer.ifremer.fr/doc/1998/publication-383.pdf DOI:10.1016/S0044-8486(98)00380-9 http://archimer.ifremer.fr/doc/00000/383/ | Partager |
La biosécurité à l'Ifremer LEAD,station de Saint-Vincent Auteur(s) : Herlin, Jose Résumé : Penaeus stylirostris shrimp imported from Hawaii for a genetic program and kept at the Saint-Vincent research facility had progressively developed a high sensitivity to IHHNV, the etiological agent of the Infectious, Hypodermic, and Hematopoietic Necrosis. Fears were that the virus could not only be transferred to their offspring, but also that the New Caledonian strain of Penaeus stylirostris, so far considered as resistant, could become sensitive by an off balance of its hosts/pathogen equilibrium. In October 2008, a joint decision between Uprac (shimp owner), Davar (New Caledonian Animal Health Authority) and Ifremer (scientific partner) was taken to terminate all pure breed and hybrid Hawaiian populations remaining in the ponds, and to implement an intensive fallowing protocol, and controlled restocking. IHHNV was also suspected to trouble experiments carried out in the research facility (mortalities, deformities, slow growth), thus affecting research programs. Difficulties pointed out by IHHNV have triggered since 2008 some awareness on biosecurity issues at some level of the New Caledonian shrimp industry. At the St-Vincent research station, it led to a risk assessment and implementation of biosecurity procedures (fallowing, propagation, grow out and use of screened animals) which minimizes the risk of expression of IHHNV.
The use of a brand new hatchery building on site also helped in resetting routine hygiene procedures during production and in controlling incoming and outgoing items (shrimp, water, feed, personnel, equipment, pests and wastes).
IHHNV has not been detected on analysed samples since then nor have any symptoms been found during experimental or broodstock growout. Keeping this control alive is considered as a useful tool to lower the risk of potential health threats, to get a better understanding in case of an outbreak, and to increase the reliability of experimental results. Les crevettes originaires d’Hawaii et conservées au LEAD St-Vincent, dans le cadre du programme d’introduction de variabilité génétique dans le cheptel Calédonien, ont progressivement montré une sensibilité importante au virus IHHNV, agent de la Nécrose Hypodermale et Hématopoïétique Infectieuse (mortalité, croissance ralentie, déformations). Les risques d’une transmission génétique de cette sensibilité à leur descendance à moyen terme, et de son développement par rupture de l’équilibre hôte/pathogène sur la souche dite « Calédonienne » jusqu’alors considérée comme résistante à l’IHHN, ont conduit à partir d’octobre 2008, avec l’accord et l’appui de l’UPRAC et de la DAVAR, à la suppression des animaux hawaiiens ou hybrides encore présents dans les bassins de terre du LEAD St-Vincent, au vide sanitaire de ces derniers, et au repeuplement contrôlé du site. D’autre part, le virus IHHN est soupçonné d’avoir pu affecter les animaux utilisés dans les expérimentations menées au LEAD-St-Vincent (mortalités, déformations, croissance réduite), influençant donc potentiellement leur bon déroulement et celui des programmes de recherches. Les obstacles soulevés par l’expression du virus IHHN ont donc initié depuis 2008 une prise de conscience et la mise en place de démarches de biosécurité dans certains points de la filière. A la station de St-Vincent, cette démarche s’est traduite par une identification/évaluation des risques et l’application de mesures (vide sanitaire, reproduction, élevage et utilisation des animaux contrôlés) chacune dotée de procédures qui regroupées, minimisent le risque d’introduction et de dissémination de l’IHHNV. La prise en main de la nouvelle structure d’écloserie sur le site de la SASV a également été mise à profit pour revoir les procédures d’hygiène de routine dans la conduite des productions et dans le contrôle des flux intrants et sortants (animaux, eau, aliments, personnel, équipement, nuisibles, déchets). Aucune détection d’IHHNV sur les échantillons prélevés n’a depuis été observée, de même qu’aucun symptôme de l’expression de cette maladie sur les animaux en élevage sur le site de Saint-Vincent. La poursuite de ces mesures est envisagée comme un outil d’aide à la minimisation des problèmes potentiels, à leur compréhension en cas d’apparition, et à la fiabilisation des résultats. Droits : 2010 Ifremer http://archimer.ifremer.fr/doc/00118/22949/20776.pdf http://archimer.ifremer.fr/doc/00118/22949/ | Partager |
Contribution à l'amélioration des survies et performances de reproduction de L. stylirostris en saison fraîche en Nouvelle-Calédonie Auteur(s) : Pham, Dominique Wabete, Nelly Lemaire, Pierrette Mailliez, Jean-rene Broutoi, Francis Chim, Liet Résumé : Since 1993, the New Caledonian shrimp industry has been faced with cold season mortality outbreaks of multifactorial origin. This pathology called “Syndrome 93” affects as well animals in grow-out ponds as broodstock after their transfer in maturation room, with up to 35% of mortality in 48 hours. From literature data and physiological studies on Litopenaeus stylirostris, several combinations of three abiotic parameters (temperature, salinity and feeding) have been tested during the breeders stocking period. This research allowed us to lower stress factors impact and to propose a transfer protocol which leads to an optimal physiological comfort of the animal. Results showed that survival rate is improved and spawning rates are twice higher. These findings have come up with a routine protocol of carrying out any transfer in a tank with water at temperature of 26°C, salinity of 26 ppt and no food supply during 48 hours. The use of this protocol in a commercial hatchery led to a significant broodstock survival improvement. Depuis 1993, la filière crevetticole calédonienne est confrontée aux mortalités de saison froide dont les causes sont multifactorielles. Cette pathologie appelé « Syndrome 93 » affecte non seulement les animaux dans les bassins de grossissement, mais elle touche également les géniteurs après leur transfert en salle de maturation, les pertes pouvant atteindre 35% en 48 heures. A partir de la littérature et d’études physiologiques de la Litopenaeus stylirostris, différentes combinaisons de trois paramètres abiotiques (température, salinité et alimentation) ont été testés lors de la stabulation des reproducteurs en salle de maturation. Ces recherches ont permis de diminuer l’impact des facteurs de stress et d’établir un protocole de transfert des animaux dans les conditions de confort physiologique optimale. Les résultats montrent que la survie est améliorée mais que le pourcentage de pontes est également doublé. Il est maintenant recommandé pour tout transfert, de prévoir des bacs de réception dans lesquels l’eau est à la température de 26°C, la salinité à 26 ppt et de ne fournir aucune alimentation pendant 48 heures. L’application de ce protocole dans une écloserie privée a démontré un gain substantiel sur la survie des géniteurs. Droits : 2008 Ifremer http://archimer.ifremer.fr/doc/00118/22970/20800.pdf http://archimer.ifremer.fr/doc/00118/22970/ | Partager |
Marine crustacean farming: present status and perspectives Auteur(s) : Laubier, Annie Laubier, Lucien Éditeur(s) : EDP Sciences Résumé : For centuries, several species of prawns and crabs have been raised from wild-caught juveniles in coastal brackish-water fish ponds in various countries of south east Asia. The Indonesian "tambaks" are well known examples of such traditional practices. In western countries, since the turn of the century, advances of marine biology and fast increase of marine fisheries enabled the development of large-scale production and release of larval stages of American and European lobsters in a fruitless attempt to restock natural populations. After the Second World War, the increasing demand for crustaceans in United States and Japan was satisfied by opening new prawn fisheries all over the world. A major breakthrough was achieved with the development of hatchery technologies for the penaeid prawn Penaeus japonicus (Hudinaga, 1942 in Japan) and the caridean prawn Macrobrachium rosenbergii (Ling, 1969 in Malaysia), which occurred during the first decades of the second half of this century. Together with the increasing market demand in developed countries for sea food, this led to a considerable interest of both public agencies and private investors in marine shrimp and prawn culture. In western countries, a large number of pioneering commercial ventures, often based on assumptions not scientifically founded, failed. Nevertheless, the aquaculture production of prawns mainly based on wild-caught juveniles increased in South-East Asia and Central America during the 1980s. This overall positive trend should not hide important failures which occurred at a local scale, such as the Taiwanese crisis of 1988 due principally to environmental degradation, resulting in severe disease problems and a near collapse of the farming activity. Following the early period of hatchery technology development, the major scientific achievements were related to food requirements and formulation of compound diets for larvae, juveniles and adults and to a better knowledge of diseases caused by bacteria and several viruses which have been identified from hatcheries and intensive farming ponds. Additional new technological advances have emerged from recent research in the fields of physiology (endocrinology) and genetics. By far, the major part of the world production of marine crustaceans relies on penaeid prawns and, to a lesser extent, on Macrobrachium species. However, some other species of marine crustaceans have potential for aquaculture. The economic aspects of marine crustacean aquaculture should be considered together with those of the fishing industry: market prices are rather similar, depending on the quality of the product. The balance between market demand and production is an important constraint which, in turn, establishes the success of prawn farming. Since the early 1980s, crustacean aquaculture has increased tremendously in both Asia and America: the world production for 1991 approximated 700,000 tons, with more than 600,000 tons from penaeid prawn culture. Pendant des siècles, quelques espèces de crevettes et de crabes ont été élevées à partir d'individus juvéniles capturés dans le milieu naturel, dans des bassins à poissons d'eau saumâtre de différents pays d'Asie du Sud-Est. Les « tambaks » indonésiens sont des exemples bien connus de ces pratiques traditionnelles. Dans les pays occidentaux, depuis le début du siècle, les progrès réalisés en biologie marine et la croissance rapide des pêches maritimes ont permis le développement de la production à grande échelle de stades larvaires des homards européen et américain, dans une vaine tentative de reconstitution des stocks naturels. Après la Seconde Guerre mondiale, la demande croissante des Etats-Unis et du Japon a été satisfaite par l'ouverture de nouvelles pêcheries de crevettes dans l'océan mondial. Un progrès décisif a été réalisé avec la mise au point des techniques d'écloserie pour la crevette Pénaeidé Penaeus juponicus (Hudinaga, 1942 au Japon) et la crevette Caridé Macrobrachium rosenbergii (Ling, 1969 en Malaisie), qui sont apparues au cours des premières décennies de la seconde moitié de ce siècle. Parallèlement à la demande croissante du marché des pays développés pour les produits de la mer, ce résultat s'est traduit par un intérêt considérable porté à l'élevage des crevettes marines de la part des institutions de recherche et des investisseurs. Dans les pays occidentaux, un grand nombre de tentatives d'élevage commerciales, fondées sur des considérations non scientifiquement établies, ont échoué. Néanmoins, la production aquacole de crevettes reposant sur la capture d'animaux juvéniles dans la nature, s'est développée en Asie du Sud-Est et en Amérique Centrale au cours des années 1980. Cette tendance globalement positive ne doit pas dissimuler les faillites importantes qui se sont produites à l'échelle locale, comme la crise des élevages de Taiwan en 1988, causée principalement par une dégradation de l'environnement, entraînant l'apparition de problèmes pathologiques sévères et une disparition presque totale de l'activité d'élevage. Après la période pionnière du développement de la technologie d'écloserie, les principaux acquis scientifiques concernent les besoins alimentaires et la formulation d'aliments composés pour les larves, les jeunes et les adultes, et une meilleure connaissance des maladies bactériennes et virales qui interviennent dans les écloseries et les fermes d'élevage intensif. De nouveaux progrès techniques complémentaires ont vu le jour à la suite de recherches dans les domaines de la physiologie (endocrinologie) et de la génétique. De loin, la majeure partie de la production mondiale de Crustacés marins repose sur les crevettes Pénaeidés et, dans une moindre mesure, sur des espèces de Macrobrachium. Cependant, quelques autres espèces de Crustacés marins intéressent potentiellement l'aquaculture. Les aspects économiques de l'aquaculture de crustacés marins doivent être considérés avec ceux de l'industrie des pêches maritimes : les prix du marché sont à peu près les mêmes, et dépendent de la qualité du produit. Le rapport entre la demande du marché et la production est une contrainte importante qui à son tour, détermine les conditions du succès de l'élevage de crevettes. Depuis le début des années 1980, l'aquaculture de crustacés a augmenté considérablement en Asie et en Amérique : la production mondiale pour 1991 avoisine 700 000 tonnes, avec plus de 600 000 tonnes provenant des élevages de crevettes Pénaeidés. Aquatic Living Resources (0990-7440) (EDP Sciences), 1993-10 , Vol. 6 , N. 4 , P. 319-329 Droits : IFREMER-Gauthier-Villars, 1993 http://archimer.ifremer.fr/doc/00190/30119/28587.pdf DOI:10.1051/alr:1993033 http://archimer.ifremer.fr/doc/00190/30119/ | Partager Voir aussi Crustacés crevettes crabes homards aquaculture exploitation Penaeidae Crustaceans shrimps prawns Télécharger |
Stylog : base de données pour le suivi des élevages de crevettes de Nouvelle-Calédonie Auteur(s) : Soulard, Benoit Frappier, Julie Herlin, Jose Beliaeff, Benoit Éditeur(s) : 11th Pacific Science Inter-Congress / 2nd Symposium on French Research in the Pacific, Tahiti Résumé : In order to have a standardised database gathering most information on New Caledonian prawn farms a software, called “Stylog-module ferme”, was developed and implemented by Ifremer with the help of shrimp farmers. Data recording results in more than 2 million data from 250 crops since the beginning of the project (2003). The main objective is to help the researchers in understanding the processes taking place in a cultured pond. It also provides a follow-up of the prawn farming industry (evolution of management practices, health surveillance system…) and to facilitate sharing of information between the different partners of the industry (prawn farmers, research and local authorities). Finally, this decision-making tool also helps shrimp farmers for routine crop management. This poster presents the method and tools used to create this database, from the definition of specifications, in close relation with end users, to the implementation of a real project structure, including all partners (users committee and steering committee, agreement of confidentiality). It also shows some examples resulting from the analysis of these data (investigating good management practices indicators, publishing technical bulletins or specific data analysis for research…). Finally, the networking (Extranet) of this base seems to present real perspectives in sharing information, and analysis capacity. Afin de disposer d'une base de données regroupant un maximum d'informations collectées sur les fermes de grossissement de crevettes de Nouvelle-Calédonie, dans un format informatisé et standardisé, un logiciel nommé « Stylog – module ferme » a été développé par l'Ifremer en lien avec les aquaculteurs. L'archivage des données a permis de collecter depuis le début de sa conception (2003) plus de 2 millions de données représentant près de 250 élevages. Les objectifs principaux de cette base sont d'aider les chercheurs dans la compréhension de phénomènes pouvant survenir dans un bassin d'élevage, d'assurer un suivi de la filière (évolution des pratiques zootechniques, veille clinique…) et de faciliter les échanges d'informations entre les différents partenaires de la filière (aquaculteurs privés, recherche, institutions). Enfin, cet outil d'aide à la décision permet aux aquaculteurs de gérer leurs élevages au quotidien. Ce poster explique d'une part, la méthode et les outils utilisés pour créer cette base de données, de la réalisation du cahier des charges, en lien étroit avec les utilisateurs finaux, à la mise en place d'une réelle structure projet regroupant les différents partenaires (comité des utilisateurs et comité de pilotage, convention de confidentialité). D'autre part, il présente quelques exemples de résultats en termes d'utilisation de ces données (recherche d'indicateurs de qualité d'élevage, édition de bulletins techniques, analyses de données spécifiques à la recherche…). Enfin, la mise en réseau (Extranet) de cette base semble présenter de réelles perspectives en termes de partage de l'information et de capacité d'analyses. Droits : 2009 Ifremer http://archimer.ifremer.fr/doc/00065/17659/15189.pdf http://archimer.ifremer.fr/doc/00065/17659/ | Partager |