Éditeur(s) : HAL CCSD
Résumé : Pregnancy is a sensitive and critical period for the development of the child and the health of adults-to-be. The biological and physiological adaptation of the body dealing physical and psychosocial stressors during this period may exert its effects in adulthood (and possibly over several generations). This delayed toxicity presupposes intrinsically the need to study the effects of exposure to environmental risk factors during fetal life using a holistic approach involving risk factors at both the micro (individual characteristics) and the macro level (physical and psycho-social context). Given the heterogeneity of the Breton territory in which this work was conducted, we explored whether the impact of social inequalities and the physical environment (air pollution) on birth outcomes (fetal growth and the risk of prematurity) could be modified according to an urban or rural place of residence. This work was based on data collected as part of the Breton mother-child cohort PELAGIE, which had included 3421 pregnant women between 2002- 2006. The anthropometric parameters and gestational age at birth were measured by medical personnel at delivery. We defined urban and rural areas according to the definition of “urban units” from the National Census Bureau (INSEE). The annual concentrations of air pollution (nitrogen dioxide (NO2)) were estimated using a land-use regression modeled at a 100 m scale and developed as part of an European project. Finally, neighbourhood deprivation was estimated using a composite index developed at census blocks level and whose use was legitimated over both urban and rural areas. Neighbourhood deprivation was associated with an increased risk of infants with fetal growth restriction, only for women living in rural areas. We also observed an increased risk of preterm birth associated with NO2 concentrations > 16.4 μg.m-3, only among women residing in urban areas. The associations between air pollution and fetal growth, although sex-specific, did not seem on the other hand, to vary significantly according to the urban-rural spectrum. This work confirms the need to explore the influence of both social and environmental inequalities on intrauterine development, and to assess the role of place-based factors, such as the urban-rural context, in shaping these inequalities.
La grossesse est une période sensible et déterminante pour le développement de l’enfant et l’état de santé à l’âge adulte. L'adaptation biologique et physiologique de l'organisme face à des « stresseurs » physiques et psychosociaux au cours de cette période peut ainsi exercer ses effets à l'âge adulte (et possiblement sur plusieurs générations). Cette toxicité différée suppose intrinsèquement la nécessité d’étudier les conséquences des expositions environnementales au cours de la vie foetale et ce suivant une approche holistique intégrant autant les facteurs de risque à des niveaux micro (caractéristiques individuelles) et macro (expositions physiques externes et contexte psycho-social). Etant donné l’hétérogénéité du territoire breton dans lequel s’inscrit ce travail de thèse, nous avons cherché à explorer l’impact des inégalités sociales et de l’environnement physique (pollution atmosphérique) sur l’issue de la grossesse (croissance foetale et risque de prématurité) indépendamment chez des femmes enceintes résidant dans des zones urbaines et rurales. Ce travail s’est appuyé sur les données issues de la cohorte bretonne mère-enfant PELAGIE, qui a inclus 3421 femmes enceintes entre 2002- 2006. Les paramètres anthropométriques et l’âge gestationnel à la naissance ont été renseignés à l’accouchement par le personnel médical. Le contexte urbain et rural breton a été caractérisé à partir de la définition des unités urbaines de l’INSEE. Les concentrations annuelles de pollution atmosphérique (dioxyde d’azote (NO2)) ont été modélisées à une échelle de 100 m à partir d’un modèle de "land-use regression" développé à l’échelle européenne. Enfin, le niveau socio-économique des IRIS a été estimé à l’aide d’un indice de désavantage social - construit à partir des données du recensement de l’INSEE et dont la validité et l’adaptabilité à des territoires à la fois urbains et ruraux a été examinée au préalable. Nos résultats suggèrent une influence délétère d’un contexte de vie socioéconomique défavorable sur la croissance intra-utérine, spécifiquement chez les femmes résidant en milieu rural. Nous avons également observé une augmentation du risque de prématurité associée à des niveaux > 16.4 μg.m-3 de NO2 dans l’air, à l’inverse, uniquement chez les femmes résidant dans des zones urbaines. Les associations entre l’exposition à la pollution atmosphérique et les marqueurs de la croissance intra-utérine, bien que sexe-spécifiques, ne semblent en revanche pas varier sensiblement suivant le gradient urbain-rural. Ce travail confirme la nécessité d’évaluer l’influence des inégalités sociales et environnementales sur le développement intra-utérin et de considérer l’importance et le rôle du contexte de vie, notamment urbain-rural, dans la formation de ces inégalités.
https://tel.archives-ouvertes.fr/tel-01308518

NNT : 2015REN1B010
tel-01308518
https://tel.archives-ouvertes.fr/tel-01308518
https://tel.archives-ouvertes.fr/tel-01308518/document
https://tel.archives-ouvertes.fr/tel-01308518/file/BERTIN_M--lanie.pdf

Résumé : This book represents a two year study by a team of French biologists to collect, summarise and present the experience and knowledge gained in the rearing of the giant freshwater prawn, not only in the French overseas territories but also in the main countries which are developing this culture. This document comprises four parts : - The first describes the world market. The prawn rearing is already an economic reality in the tropical world, the United States and in Europe. - The second on shows that, although the culture techniques are quite complex, production systems can be adapted to local conditions and markets. - The third part is a practical guide, both for investors and farmers, giving all necessary data to start-up and monitor a project in the best conditions. - The final part summarises the main elements of the biology of this species, in order that people interested in its development may have a better understanding of its life history. The authors were based at the aquaculture laboratory of the Ifremer station of French Guiana from 1989 to 1990 and had 15 years previous experience of this culture in Tahiti and the Caribbean. Regarding this initial complete French synthesis on this species in a special care has been given to balance accurate scientific reporting with the objective of reaching a wider audience.
Quinze années d'expérience sur l'élevage de la crevette d'eau douce tropicale, à Tahiti, aux Antilles et en Guyane, comme dans les principaux pays qui pratiquent cet élevage, sont rassemblées et synthétisées dans cet ouvrage. - Où en est le marché mondial ? Cet élevage est déjà une réalité économique dans le monde tropical, comme sur les marchés américain ou européen. - L'exploitation du cheptel est complexe, mais des schémas de production peuvent être adaptés aux conditions locales d'élevage et de marché. - Ce guide pratique donne toutes les informations nécessaires pour monter un projet et le mettre en oeuvre dans de bonnes conditions. - Pour élever la crevette, il faut mieux la connaître. On trouve ici les éléments essentiels de la biologie de cette espèce. L'ouvrage a été rédigé par l'équipe du laboratoire d'aquaculture de la station Ifremer de Guyane en 1989 et 1990. Ce guide, première synthèse en langue française, est unique en son genre. Il équilibre rigueur scientifique et esprit de vulgarisation pour s'adresser aussi bien aux scientifiques et techniciens qu'aux éleveurs ou aux décideurs du développement.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1991/rapport-639.pdf
http://archimer.ifremer.fr/doc/00000/639/

Éditeur(s) : HAL CCSD
Résumé : International audience
Trees can control their shape and resist gravity thanks to their ability to produce wood under tensile stress. This stress is known to be produced during the maturation of wood fibres but the mechanism of its generation remains unclear. This study focuses on the formation of the secondary wall in tension wood produced in artificially tilted poplar saplings. Thickness of secondary wall layer (SL) and gelatinous layer (GL) were measured from cambium to mature wood in several trees sampled at different times after tilting. Measurements on wood fibres produced before tilting show the progressive increase of secondary wall thickness during the growing season. After the tilting date, SL thickness decreased markedly from normal wood to tension wood while the total thickness increased compared to normal wood, with the development of a thick GL. However, even after GL formation, SL thickness continues to increase during the growing season. GL thickening was observed to be faster than SL thickening. The development of the unlignified GL is proposed to be a low cost, efficient strategy for a fast generation of tensile stress in broadleaved trees.
IAWA journal / International Association of Wood Anatomists.

hal-01229277
https://hal.archives-ouvertes.fr/hal-01229277
https://hal.archives-ouvertes.fr/hal-01229277/document
https://hal.archives-ouvertes.fr/hal-01229277/file/AbediniClair-Iawa-TensionCellWallThickening_preprint_HAL.pdf
DOI : 10.1163/22941932-00000084

Éditeur(s) : HAL CCSD National Shellfisheries Association
Résumé : International audience
Monitoring gonad maturation for protandrous and functional hermaphrodite species such as the giant clam Tridacna maxima is difficult due to the juxtaposition and relative proportion of male and female tissues in the gonad [gonadal sex ratio (GSR)]. Here, the relevance of the widely used gonadosomatic index (GSI) as proxy of giant clam gonad maturation is tested with a large dataset (n ¼ 265). Gonadosomatic index is compared with other indices, namely the proportion of the male part harboring spermatozoids, the proportion of empty oocyte follicles, the mean oocyte diameter, and the oocyte elongation. At gonad scale, high index variability highlighted partial spawning. At individual scale, male and female maturation proxies were contrasted, showing either asynchronous emissions of male and female gametes or contrasted spermatogenesis and oogenesis duration. The GSI was mostly driven by the number and diameter of oocytes and therefore it is recommended here as primary proxy for female maturity. Except for the oocyte elongation, all indices were affected by the GSR, which ruled out drawing conclusions at population scale. These results highlight the need for maturation stage proxies that are optimized for functional hermaphrodite species.
ISSN: 0730-8000

hal-01613648
https://hal.archives-ouvertes.fr/hal-01613648
https://hal.archives-ouvertes.fr/hal-01613648/document
https://hal.archives-ouvertes.fr/hal-01613648/file/Gaertner-Mazouni-2016-eio.pdf
DOI : 10.2983/035.035.0107

Éditeur(s) : HAL CCSD
Résumé : Juin 2003
Dans le contexte actuel de mondialisation, de changement climatique et de forte anthropisation de la biosphère, les Petites Antilles s'érigent en monde marginal ou encore en anti-monde. Fracturées du point de vue politique, économique, linguistique et à certains égards culturel, ces territoires insulaires du Bassin caraïbe recèlent de richesses environnementales (physiques, écosystèmiques et paysagères). Comme partout ailleurs dans le monde humanisé, la survie de ces sociétés implique le rétablissement des grands équilibres écologiques consubstantiels à une articulation efficiente entre le naturel, l'urbain et l'agricole. Compte tenu de la pression démographique, variable d'une île à l'autre, la pérennité des écosystèmes en covariance avec un développement économique profitant au plus grand nombre ne peut se concevoir à l'échelle insulaire. Il semble judicieux d'imaginer un développement à l'échelle de l'archipel basé sur la complémentarité, puisque au sein de chaque composante les diverses ressources biologiques terrestres et marines sont insuffisantes pour assurer une autosuffisance en termes de produits de premières nécessités et d'énergie. Le développement des Petites Antilles s'il doit être durable et prendre comme ossature les directives de Rio (Brésil, 1992) et de Johannesburg (Afrique du Sud, 2002), il devra s'inscrire dans une gestion administrative, scientifique, technique et agricole commune dans le cadre d'une Caraïbe fonctionnellement unifiée. A l'unité floristique, faunistique et biocénotique doivent correspondre des stratégies et donc des politiques, similaires mais diversifiées, d'utilisation des ressources abiotiques (énergie éolienne, hydroélectrique et photovoltaïque) et biotiques (écosystèmes terrestres et marins, flore, faune, sols). Malheureusement dans le présent, la compartimentation économique et politique de la Caraïbe rend ces propos profondément utopiques. Néanmoins dans le futur la survie des peuples des Petites Antilles, impliquera un développement durable signifié par des rapports équilibrés entre hommes et milieux.
–––––
In the nowadays context of "globalization", climatic variation and great anthropization of the biosphere, the Lesser Antilles appear as a marginal world. In a political point of view, those islands offer a greatly distinct and fractured image. The same fact can also be observed in an economic, linguistic and, in a certain measure, cultural perspective. Despite of such a statement, those territories of the Caribbean basin keep economic and landscape resources whom survival depends on the re-establishment of the great ecologic structures implying a productive articulation between the natural, the urban and the agricultural. It seems pertinent to imagine a development on the level of the archipelago based upon complementarity since each constituent presents a limited number of earth and marine biologic reserves which do not guarantee an autosubsistance in energy and first nessecity productivity. A lasting development of the Lesser Antilles supposes a necessary and commun administrative, scientific, technical and agricultural management within the setting of a Caribbean functionally unified. Facing the biocenotic unity, a useful strategy, based on a similar and diversified organization in the exploitation of biophisic resources, must be erected. Such statements could be considered as utopic, regarding the situation of politic and economic division in the Caribbean nowadays. However, in the future, the survival of those insular societies will imply a lasting development lying upon an efficient relationship between Man and environment.
https://halshs.archives-ouvertes.fr/halshs-00003054

Droits : info:eu-repo/semantics/OpenAccess
halshs-00003054
https://halshs.archives-ouvertes.fr/halshs-00003054
https://halshs.archives-ouvertes.fr/halshs-00003054/document
https://halshs.archives-ouvertes.fr/halshs-00003054/file/Devpt-durable_JOSEPH-PHILIPPE.pdf

Éditeur(s) : HAL CCSD BioScientifica
Résumé : International audience
Paracetamol/acetaminophen (N-Acetyl-p-Aminophenol; APAP) is the preferred analgesic for pain relief and fever during pregnancy. It has therefore caused concern that several studies have reported that prenatal exposure to APAP results in developmental alterations in both the reproductive tract and the brain. Genitals and nervous system of male mammals are actively masculinised during foetal development and early postnatal life by the combined actions of prostaglandins and androgens, resulting in the male-typical reproductive behaviour seen in adulthood. Both androgens and prostaglandins are known to be inhibited by APAP. Through intrauterine exposure experiments in C57BL/6 mice, we found that exposure to APAP decreased neuronal number in the sexually dimorphic nucleus (SDN) of the preoptic area (POA) in the anterior hypothalamus of male adult offspring. Likewise, exposure to the environmental pollutant and precursor of APAP, aniline, resulted in a similar reduction. Decrease in neuronal number in the SDN-POA is associated with reductions in male sexual behaviour. Consistent with the changes, male mice exposed in uteri to APAP exhibited changes in urinary marking behaviour as adults and had a less aggressive territorial display towards intruders of the same gender. Additionally, exposed males had reduced intromissions and ejaculations during mating with females in oestrus. Together, these data suggest that prenatal exposure to APAP may impair male sexual behaviour in adulthood by disrupting the sexual neurobehavioral programming. These findings add to the growing body of evidence suggesting the need to limit the widespread exposure and use of APAP by pregnant women.
ISSN: 1470-1626

hal-01560285
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560285
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560285/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560285/file/Prenatal%20exposures%20to%20paracetamol-accepted.pdf
DOI : 10.1530/REP-17-0165

Éditeur(s) : ICES 2008 Annual Science Conference, 22-26 september 2008, HALIFAX, CANADA
Résumé : The development on the ecosystem-based approach to fisheries implies to improve integrated analysis of fisheries by considering the biological as well as the socio-economic dimensions of the exploitation and thus, an improvement in the knowledge of fleets structure, evolution and activity. Since 2000, Ifremer has been implemented a Fisheries Information System (FIS), a multidisciplinary monitoring network allowing an integrated and comprehensive view of fishery systems including biological, technical, environmental and economical components. The FIS covers all the French fisheries, including the so-often neglected small-scale fisheries. One of the originalities of the FIS lies in the fleet monitoring procedure: a comprehensive collection of annual activity calendars aiming at characterizing the inactivity or activity of the vessels each month of the year and, in the latter case, the métiers practised (use of a gear to target one or several species) and the main fishing areas. This survey covers all the French fishing fleets and provides minimum but exhaustive information on the vessels. It is particularly instructive for the small-scale fisheries, where catches and effort data are often incomplete. Furthermore, this exhaustive data allows stratifying the fleet in fleets (typologies) and thus provides the basis (i) for the implementation of sampling schemes to estimate catches, landings, discards or economic performance of the different fleets and (ii) for the development of a fleet-métier matrix giving the possibility of identifying at the time, the structure of the whole fleet in fleets, the métier polyvalence of the vessels and the allocation of fishing effort on the different exploited fishing resources.

Droits : 2008 ICES
http://archimer.ifremer.fr/doc/00059/16996/14498.pdf
http://archimer.ifremer.fr/doc/00059/16996/

Éditeur(s) : Pergamon-elsevier Science Ltd
Résumé : The EUNIS (European Union Nature Information System) habitat classification system aims to provide a common European reference set of habitat types within a hierarchical classification, and to cover all terrestrial, freshwater and marine habitats of Europe. The classification facilitates reporting of habitat data in a comparable manner, for use in nature conservation (e.g. inventories, monitoring and assessments), habitat mapping and environmental management. For the marine environment the importance of a univocal habitat classification system is confirmed by the fact that many European initiatives, aimed at marine mapping, assessment and reporting, are increasingly using EUNIS habitat categories and respective codes. For this reason substantial efforts have been made to include information on marine benthic habitats from different regions, aiming to provide a comprehensive geographical coverage of European seas. However, there still remain many concerns on its applicability as only a small fraction of Europe's seas are fully mapped and increasing knowledge and application raise further issues to be resolved. This paper presents an overview of the main discussion and conclusions of a workshop, organised by the MeshAtlantic project, focusing upon the experience in using the EUNIS habitats classification across different countries and seas, together with case studies. The aims of the meeting were to: (i) bring together scientists with exper_ience_in the use of the EUNIS marine classification and representatives from the European Environment Agency (EEA); (ii) agree on enhancements to EUNIS that ensure an improved representation of the European marine habitats; and (iii) establish practices that make marine habitat maps produced by scientists more consistent with the needs of managers and decision-makers. During the workshop challenges for the future development of EUNIS were identified, which have been classified into five categories: (1) structure and hierarchy; (2) biology; (3) terminology; (4) mapping; and (5) future development. The workshop ended with a declaration from the attendees, with recommendations to the EEA and European Topic Centre on Biological Diversity, to take into account the outputs of the workshop, which identify weaknesses in the current classification and include proposals for its modification, and to devise a process to further develop the marine component of the EUNIS habitat classification. (C) 2012 Elsevier Ltd. All rights reserved.
Marine Pollution Bulletin (0025-326X) (Pergamon-elsevier Science Ltd), 2012-12 , Vol. 64 , N. 12 , P. 2630-2638

Droits : 2012 Elsevier Ltd. All rights reserved.
http://archimer.ifremer.fr/doc/00125/23663/22686.pdf
DOI:10.1016/j.marpolbul.2012.10.010
http://archimer.ifremer.fr/doc/00125/23663/

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Acquiring information is indisputably energy-consuming and conversely, the availability of information permits greater efficiency. Strangely, the scientific community long remained reluctant to establish a physical equivalence between the abstract notion of information and sensible thermodynamics. However, certain physicists such as Szilard and Brillouin proposed: (i) to give to information the status of a genuine thermodynamic entity (k B T ln2 joules/bit) and (ii) to link the capacity of storing information inferred from correlated systems, to that of indefinitely increasing organization. This positive feedback coupled to the self-templating molecular potential could provide a universal basis for the spontaneous rise of highly organized structures, typified by the emergence of life from a prebiotic chemical soup. Once established, this mechanism ensures the longevity and robustness of life envisioned as a general system, by allowing it to accumulate and optimize microstate-reducing recipes, thereby giving rise to strong nonlinearity, decisional capacity and multistability. Mechanisms possibly involved in priming this cycle are proposed.
ISSN: 0169-6149

inserm-00826658
http://www.hal.inserm.fr/inserm-00826658
http://www.hal.inserm.fr/inserm-00826658/document
http://www.hal.inserm.fr/inserm-00826658/file/OLEB-2013.pdf
DOI : 10.1007/s11084-013-9329-2

Éditeur(s) : HAL CCSD American Society for Biochemistry and Molecular Biology
Résumé : International audience
alpha- and beta-spectrins are components of molecular scaffolds located under the lipid bilayer and named membrane skeletons. Disruption of these scaffolds through mutations in spectrins demonstrated that they are involved in the membrane localization or the maintenance of proteins associated with them. The ubiquitous alphaII-spectrin chain bears in its central region a unique domain that is sensitive to several proteases such as calpains or caspases. The conservation of this region in vertebrates suggests that the proteolysis of alphaII-spectrin by these enzymes could be involved in important functions. To assess the role of alphaII-spectrin cleavage in vivo, we generated a murine model in which the exons encoding the region defining this cleavage sensitivity were disrupted by gene targeting. Surprisingly, homozygous mice expressing this mutant alphaII-spectrin appeared healthy, bred normally, and had no histological anomaly. Remarkably, the mutant alphaII-spectrin assembles correctly into the membrane skeleton, thus challenging the notion that this region is required for the stable biogenesis of the membrane skeleton in nonerythroid cells. Our finding also argues against a critical role of this particular alphaII-spectrin cleavage in either major cellular functions or in normal development.
ISSN: 0021-9258

inserm-00284621
http://www.hal.inserm.fr/inserm-00284621
http://www.hal.inserm.fr/inserm-00284621/document
http://www.hal.inserm.fr/inserm-00284621/file/Meary_-_JBC_2007.pdf
PUBMED : 17374614

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The tg(cyp19a1b-GFP) transgenic zebrafish expresses GFP (green fluorescent protein) under the control of the cyp19a1b gene, encoding brain aromatase. This gene has two major characteristics: (i) it is only expressed in radial glial progenitors in the brain of fish and (ii) it is exquisitely sensitive to estrogens. Based on these properties, we demonstrate that natural or synthetic hormones (alone or in binary mixture), including androgens or progestagens, and industrial chemicals induce a concentration-dependent GFP expression in radial glial progenitors. As GFP expression can be quantified by in vivo imaging, this model presents a very powerful tool to screen and characterize compounds potentially acting as estrogen mimics either directly or after metabolization by the zebrafish embryo. This study also shows that radial glial cells that act as stem cells are direct targets for a large panel of endocrine disruptors, calling for more attention regarding the impact of environmental estrogens and/or certain pharmaceuticals on brain development. Altogether these data identify this in vivo bioassay as an interesting alternative to detect estrogen mimics in hazard and risk assessment perspective.
ISSN: 1932-6203

hal-00877371
https://hal.archives-ouvertes.fr/hal-00877371
https://hal.archives-ouvertes.fr/hal-00877371/document
https://hal.archives-ouvertes.fr/hal-00877371/file/Screening_Estrogenic_Activities-accepted.pdf
DOI : 10.1371/journal.pone.0036069

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The Hedgehog (Hh) family of secreted proteins act as morphogens to control embryonic patterning and development in a variety of organ systems. Post-translational covalent attachment of cholesterol and palmitate to Hh proteins are critical for multimerization and long range signaling potency. However, the biological impact of lipid modifications on Hh ligand distribution and signal reception in humans remains unclear. In the present study, we report a unique case of autosomal recessive syndromic 46,XY Disorder of Sex Development (DSD) with testicular dysgenesis and chondrodysplasia resulting from a homozygous G287V missense mutation in the hedgehog acyl-transferase (HHAT) gene. This mutation occurred in the conserved membrane bound O-acyltransferase (MBOAT) domain and experimentally disrupted the ability of HHAT to palmitoylate Hh proteins such as DHH and SHH. Consistent with the patient phenotype, HHAT was found to be expressed in the somatic cells of both XX and XY gonads at the time of sex determination, and Hhat loss of function in mice recapitulates most of the testicular, skeletal, neuronal and growth defects observed in humans. In the developing testis, HHAT is not required for Sertoli cell commitment but plays a role in proper testis cord formation and the differentiation of fetal Leydig cells. Altogether, these results shed new light on the mechanisms of action of Hh proteins. Furthermore, they provide the first clinical evidence of the essential role played by lipid modification of Hh proteins in human testicular organogenesis and embryonic development.
ISSN: 1553-7390

hal-01063929
https://hal.archives-ouvertes.fr/hal-01063929
DOI : 10.1371/journal.pgen.1004340
PUBMED : 24784881

Éditeur(s) : HAL CCSD De Gruyter
Résumé : International audience
Coral reefs have undergone profound ecological changes over recent decades. Areas formerly covered by scleractinian coral species are now often overgrown by macroalgae. In Martinique (West Indies), this phenomenon has lead to the colonisation of numerous coral reefs by algae, amongst which Sargassum is one of the most prominent. This study focuses on potential defence molecules produced by Sargassum polyceratium. The hexane dipping method was employed to extract surface molecules on fresh material, and their bioactivities were assessed against bacteria (marine and estuarine), and marine tropical invertebrates wan annelid (Pseudonereis sp.), a bivalve (Codakia orbicularis) and a sea urchin (Diadema antillarum)x. Extracts were active against all microorganisms tested (MICs150 or 300 mg ml-1), early stages of development in Pseudonereis sp. (MICs100 mg ml-1) and embryos of C. orbicularis and D. antillarum (MICs5 mg ml-1), suggesting the production of defence compounds by S. polyceratium.
ISSN: 0006-8055

hal-00746082
https://hal.univ-antilles.fr/hal-00746082
https://hal.univ-antilles.fr/hal-00746082/document
https://hal.univ-antilles.fr/hal-00746082/file/Publication_Sargassum_bortanica_marina.pdf
DOI : 10.1515/BOT.2011.014

Éditeur(s) : Gauthier-Villars
Résumé : The tropical alga Caulerpa taxifolia (Vahl) C. Agardh has been displayed over the last fifteen years in tropical aquaria at the Oceanographic Museum, Monaco. Its accidental introduction into the natural environment dates from 1984. It developed first in the sea immediately below the museum, and resisted winter temperatures of 11 to 13-degrees-C. Gradually the population spread over all types of substrate, including rock, sand and mud, and over a wide range of depth, 3 to 35 m, on this relatively exposed site. In summer 1990 we became aware of its presence both to the east and to the west of Monaco. To the east, it now occurs on the whole of the eastern side of Cap Martin (3 km from Monaco) and is spreading over the west face. To the west we have observed it 150 km from Monaco near Toulon. In those sites which it colonized three years ago, its coverage now reaches 100 % over extensive areas in depths between 5 and 25 m. Areas supporting stands of Cymodocea nodosa and Posidonia oceanica are covered by fronds of C. taxifolia, which measure up to 45 cm in length. The development characteristics of the species in the Mediterranean, such as population density and frond length, are different from those in its native tropical areas. The rapid spreading of this alga is probably due both to sexual reproduction and to efficient vegetative reproduction. At the rate of spreading observed at present, further rapid extension of its range is to be feared. Moreover, wherever it becomes established, it considerably modifies the vegetal communities in the infralittoral zone. This species, like most of those in the genus Caulerpa, contains the toxin, caulerpenyn, which may play a role against other organisms such as grazers, epiphytes and competitors. This alga will be eaten only by certain animals such as the Mediterranean bream or saupe, Sarpa salpa. The toxin accumulated by fish which eat Caulerpa can render them unsuitable for human consumption, as their ingestion produces symptoms similar to those of Ciguatera poisoning. Never has a species so potentially harmful to the marine population been introduced into the Mediterranean. This biological pollution raises the major international problem of direct discharge of water having passed through aquaria or mariculture installations containing exotic species. As importing exotic marine species becomes technically easier, the lack of regulation risks further similar accidents concerning not only algae, but also metazoa and micro-organisms.
Oceanologica Acta (0399-1784) (Gauthier-Villars), 1991 , Vol. 14 , N. 4 , P. 415-426

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00101/21268/18879.pdf
http://archimer.ifremer.fr/doc/00101/21268/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Saccharomyces cerevisiae is an established model organism for the molecular analysis of fundamental biological processes. The genomes of numerous strains have been sequenced, and the transcriptome and proteome ofmajor phases during the haploid and diploid yeast life cycle have been determined. However, much less is known about dynamic changes of the proteome when cells switch from mitotic growth to meiotic development. We report a quantitative protein profiling analysis of yeast cell division and differentiation based on mass spectrometry. Information about protein levels was integrated with strand-specific tiling array expression data. We identified a total of 2366 proteins in at least one condition, including 175 proteins showing a statistically significant > 5-fold change across the sample set, and 136 proteins detectable in sporulating but not respiring cells. We correlate protein expression patterns with biological processes and molecular function by Gene Ontology term enrichment, chemoprofiling, transcription interference and the formation of double stranded RNAs by overlapping sense/antisense transcripts. Our work provides initial quantitative insight into protein expression in diploid respiring and differentiating yeast cells. Critically, it associates developmentally regulated induction of antisense long noncoding RNAs and double stranded RNAs with fluctuating protein concentrations during growth and development.
ISSN: 1874-3919

hal-01485858
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01485858
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01485858/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01485858/file/Becker%20et%20al.%20-%20The%20protein%20expression%20landscape%20of%20mitosis%20and%20me.pdf
DOI : 10.1016/j.jprot.2016.12.016

Éditeur(s) : HAL CCSD
Résumé : International audience
Cumulative human impacts have led to the degradation of marine ecosystems and thedecline of biodiversity in the European and contiguous seas. Effective conservationmeasures are urgently needed to reverse these trends. Conservation must entail societalchoices, underpinned by human values and worldviews that differ between the countriesbordering these seas. Social, economic and political heterogeneity adds to the challenge ofbalancing conservation with sustainable use of the seas. Comprehensive macro-regionalcoordination is needed to ensure effective conservation of marine ecosystems andbiodiversity of this region. Under the European Union Horizon 2020 framework programme,the MarCons COST action aims to promote collaborative research to support marinemanagement, conservation planning and policy development. This will be achieved bydeveloping novel methods and tools to close knowledge gaps and advance marineconservation science. This action will provide support for the development of macro-regional and national policies through six key actions: to develop tools to analysecumulative human impacts; to identify critical scientific and technical gaps in conservationefforts; to improve the resilience of the marine environment to global change and biologicalinvasions; to develop frameworks for integrated conservation planning across terrestrial,freshwater, and marine environments; to coordinate marine conservation policy acrossnational boundaries; and to identify effective governance approaches for marine protectedarea management. Achieving the objectives of these actions will facilitate the integration ofmarine conservation policy into macro-regional maritime spatial planning agendas for theEuropean and contiguous seas, thereby offsetting the loss of biodiversity and ecosystemservices in this region.
Research Ideas and Outcomes

hal-01474085
https://hal-univ-perp.archives-ouvertes.fr/hal-01474085
https://hal-univ-perp.archives-ouvertes.fr/hal-01474085/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01474085/file/RIO_article_11884.pdf
DOI : 10.3897/rio.3.e11884

Éditeur(s) : HAL CCSD
Résumé : International audience
The relationships between Philodendron solimoesense and the larvae of two chalcid wasp species, inhabiting up to 88% of the infructescences, were studied in French Guiana. Exurus sp. (Chalcidoidea, Eulophidae, Tetrastichinae), is a gall maker and its larvae develop at the expense of seeds which are transformed into galls. We estimate that two seeds are necessary for the development of one eulophid. Their impact on plant reproductive success appears to be important, since parasitized fruits produce 60% fewer seeds than nonparasitized fruits and could explain the reduced seed set observed (53%). The larvae of a Sycophila sp. (Chalcidoidea, Eurytomidae, Eurytominae) are also present in the fruits of P. solimoesense occupied by eulophid larvae. The eurytomid larvae apparently develop at the expense of about one eulophid and are responsible for the death of 39% of them by direct predation or indirectly by consuming the gall tissues. Eulophids are far more abundant than eurytomids, since parasitized fruits contain an average of 11 eulophids but only one eurytomid. As chalcid wasp emergence takes place in a closed cavity and sex ratios are strongly biased in a given gall, local mate competition may occur as observed for fig wasps within figs.
International Journal of Plant Sciences

hal-01202708
https://hal.archives-ouvertes.fr/hal-01202708

Éditeur(s) : HAL CCSD Taylor & Francis
Résumé : International audience
The origin of replication complex subunit ORC1 is important for DNA replication. The gene is known to encode a meiotic transcript isoform (mORC1) with an extended 5′-untranslated region (5′-UTR), which was predicted to inhibit protein translation. However, the regulatory mechanism that controls the mORC1 transcript isoform is unknown and no molecular biological evidence for a role of mORC1 in negatively regulating Orc1 protein during gametogenesis is available. By interpreting RNA profiling data obtained with growing and sporulating diploid cells, mitotic haploid cells, and a starving diploid control strain, we determined that mORC1 is a middle meiotic transcript isoform. Regulatory motif predictions and genetic experiments reveal that the activator Ndt80 and its middle sporulation element (MSE) target motif are required for the full induction of mORC1 and the divergently transcribed meiotic SMA2 locus. Furthermore, we find that the MSE-binding negative regulator Sum1 represses both mORC1 and SMA2 during mitotic growth. Finally, we demonstrate that an MSE deletion strain, which cannot induce mORC1, contains abnormally high Orc1 levels during post-meiotic stages of gametogenesis. Our results reveal the regulatory mechanism that controls mORC1, highlighting a novel developmental stage-specific role for the MSE element in bi-directional mORC1/SMA2 gene activation, and correlating mORC1 induction with declining Orc1 protein levels. Because eukaryotic genes frequently encode multiple transcripts possessing 5′-UTRs of variable length, our results are likely relevant for gene expression during development and disease in higher eukaryotes. © 2016 Taylor & Francis Group, LLC.
ISSN: 1547-6286

hal-01367153
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01367153
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01367153/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01367153/file/Ndt80%20activates%20the%20meiotic%20ORC1%20transcript.pdf
PUBMEDCENTRAL : PMC5013992

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Chromatin modification enzymes are important regulators of gene expression and some are evolutionarily conserved from yeast to human. Saccharomyces cerevisiae is a major model organism for genome-wide studies that aim at the identification of target genes under the control of conserved epigenetic regulators. Ume6 interacts with the upstream repressor site 1 (URS1) and represses transcription by recruiting both the conserved histone deacetylase Rpd3 (through the co-repressor Sin3) and the chromatin-remodeling factor Isw2. Cells lacking Ume6 are defective in growth, stress response, and meiotic development. RNA profiling studies and in vivo protein-DNA binding assays identified mRNAs or transcript isoforms that are directly repressed by Ume6 in mitosis. However, a comprehensive understanding of the transcriptional alterations, which underlie the complex ume6Δ mutant phenotype during fermentation, respiration, or sporulation, is lacking. We report the protein-coding transcriptome of a diploid MAT a/α wild-type and ume6/ume6 mutant strains cultured in rich media with glucose or acetate as a carbon source, or sporulation-inducing medium. We distinguished direct from indirect effects on mRNA levels by combining GeneChip data with URS1 motif predictions and published high-throughput in vivo Ume6-DNA binding data. To gain insight into the molecular interactions between successive waves of Ume6-dependent meiotic genes, we integrated expression data with information on protein networks. Our work identifies novel Ume6 repressed genes during growth and development and reveals a strong effect of the carbon source on the derepression pattern of transcripts in growing and developmentally arrested ume6/ume6 mutant cells. Since yeast is a useful model organism for chromatin-mediated effects on gene expression, our results provide a rich source for further genetic and molecular biological work on the regulation of cell growth and cell differentiation in eukaryotes.
ISSN: 1617-4615

hal-01151619
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01151619
DOI : 10.1007/s00438-015-1051-5
PUBMED : 25957495

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Different marine habitats are characterised by different soundscapes. How or which differences may be representative of the habitat characteristics and/or community structure remains however to be explored. A growing project in passive acoustics is to find a way to use soundscapes to have information on the habitat and on its changes. In this study we have successfully tested the potential of two acoustic indices, i.e. the average sound pressure level and the acoustic complexity index based on the frequency spectrum. Inside and outside marine protected areas of Moorea Island (French Polynesia), sound pressure level was positively correlated with the characteristics of the substratum and acoustic complexity was positively correlated with fish diversity. It clearly shows soundscape can be used to evaluate the acoustic features of marine protected areas, which presented a significantly higher ambient sound pressure level and were more acoustically complex than non-protected areas. This study further emphasizes the importance of acoustics as a tool in the monitoring of marine environments and in the elaboration and management of future conservation plans. Coral reef ecosystems are among the most biologically diverse and complex marine ecosystems worldwide. In addition to their biological and ecological importance, coral reefs support major economic and physical functions (e.g. food production, tourism, biotechnology development and coastal protection) that are essential for many countries 1. Unfortunately, coral reefs are severely threatened: 20% of coral reefs can no longer be defined as such, another 25% are currently endangered and another 25% will be endangered by 2050 2. The frequency and severity of natural perturbations (e.g. cyclone, outbreaks of predators, particularly the crown-of-thorns starfish [COTS] Acanthaster planci) and anthropogenic perturbations (e.g. ocean acidification, pesticides, rising seawater temperatures) on coral reefs have greatly increased worldwide in the last three decades, and, as a consequence, reef communities (fish, coral and benthic invertebrates) have suffered unprecedented levels of decline 3,4
ISSN: 2045-2322

hal-01370610
https://hal-uvsq.archives-ouvertes.fr/hal-01370610
https://hal-uvsq.archives-ouvertes.fr/hal-01370610/document
https://hal-uvsq.archives-ouvertes.fr/hal-01370610/file/2016%20Sci.Rep.pdf
DOI : 10.1038/srep33326