Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Background Experimental evidence suggests that developmental exposure to persistent organic pollutants (POP) and to some non persistent pesticides may disrupt metabolic regulation of glucose metabolism and insulin secretion, and thereby contribute to the current epidemic of obesity and metabolic disorders. Quasi-experimental situations of undernutrition in utero have provided some information. However, the evidence in humans concerning the role of the prenatal environment in these disorders is contradictory, and little is known about long-term outcomes, such as type 2 diabetes, of prenatal exposure. Objectives Our aim was to evaluate the effects of prenatal exposure to POP and organophosphate pesticides on fetal markers of glucose metabolism in a sample of newborns from the Pelagie mother–child cohort in Brittany (France). Methods Dialkylphosphate (DAP) metabolites of organophosphate pesticides were measured in maternal urine collected at the beginning of pregnancy. Cord blood was assayed for polychlorinated biphenyl congener 153 (PCB153), p,p′-dichlorodiphenyl dichloroethene (DDE) and other POP. Insulin and adiponectin were determined in cord blood serum (n=268). Results A decrease in adiponectin and insulin levels was observed with increasing levels of DDE, but only in girls and not boys. Adiponectin levels were not related to the concentrations of other POP or DAP metabolites. Decreasing insulin levels were observed with increasing PCB153 concentrations. Insulin levels increased with DAP urinary levels. Additional adjustment for BMI z-score at birth modified some of these relations. Conclusions Our observations bring support for a potential role of organophosphate pesticides and POP in alterations to glucose metabolism observable at birth
ISSN: 0013-9351

hal-01259218
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01259218
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01259218/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01259218/file/Prenatal%20exposure%20to%20persistent%20organic%20pollutants%20and%20organophosphate%20pesticides%2C%20and%20markers%20of%20glucose%20metabolism%20at%20birth.pdf
DOI : 10.1016/j.envres.2016.01.005

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Polygonal faulting is a widespread phenomenon in sedimentary basins worldwide. It changes basin-scale fluid flow patterns and alters the physical properties of the sediments making it important for hydrocarbon exploration and geohazard analysis. It is generally accepted that polygonal fault patterns derive from dewatering and compaction of the host sediments, but there is debate regarding the processes that control polygonal faulting. New multibeam-bathymetry data from the Hatton Basin, NE Atlantic, show up to 10 m deep and 200-600 m wide troughs at the seabed. They connect to each other forming polygons that are several hundred metres across, i.e. of similar size as buried polygonal fault systems observed in 3D seismic data. The troughs are symmetrical and resemble elongated pockmarks. Previously unpublished high-resolution 2D seismic data from the same area show seismic disturbance zones similar to pipes observed under pockmarks elsewhere as well as faults that have all the characteristics of polygonal fault systems. The observation of the wide disturbance zones is enigmatic, as they appear to follow the polygonal seafloor pattern. The observed extent of the polygonal sediment contraction system is substantial covering almost 37,000 km2. We calculate that some 2600 km3 of possibly carbon-bearing fluids have been expelled from this system and we expect that this will affect the benthic ecosystems, although so far there is only limited evidence for chemosynthetic habitats.
ISSN: 0025-3227

hal-00772468
https://hal.archives-ouvertes.fr/hal-00772468
DOI : 10.1016/j.margeo.2012.09.013

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Millimeter Waves (MMW) will be used in the next-generation of high-speed wireless technologies, especially in future Ultra-Broadband small cells in 5G cellular networks. Therefore, their biocompatibilities must be evaluated prior to their massive deployment. Using a microarray- based approach, we analyzed modifications to the whole genome of a human keratinocyte model that was exposed at 60.4 GHz-MMW at an incident power density (IPD) of 20 mW/cm2 for 3 hours in athermic conditions. No keratinocyte transcriptome modifications were observed. We tested the effects of MMWs on cell metabolism by co-treating MMW-exposed cells with a glycolysis inhibitor, 2-deoxyglucose (2dG, 20 mM for 3 hours), and whole genome expression was evaluated along with the ATP content.We found that the 2dG treatment decreased the cellular ATP content and induced a high modification in the transcriptome (632 coding genes). The affected genes were associated with transcriptional repression, cellular communication and endoplasmic reticulum homeostasis. The MMW/ 2dG co-treatment did not alter the keratinocyte ATP content, but it did slightly alter the transcriptome, which reflected the capacity of MMWto interfere with the bioenergetic stress response. The RT-PCR-based validation confirmed 6 MMW-sensitive genes (SOCS3, SPRY2, TRIB1, FAM46A, CSRNP1 and PPP1R15A) during the 2dG treatment. These 6 genes encoded transcription factors or inhibitors of cytokine pathways, which raised questions regarding the potential impact of long-term or chronic MMW exposure on metabolically stressed cells. © 2016 Soubere Mahamoud et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ISSN: 1932-6203

hal-01367149
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01367149
DOI : 10.1371/journal.pone.0160810
PUBMED : 27529420

Éditeur(s) : HAL CCSD
Résumé : The ovary is not solely the unique source of oocytes necessary for fertilization but also a crucial conductor of endocrine regulations of the reproductive system. In the fetal ovary, oocytes grouped in clusters are surrounded by somatic pre-granulosa cells, altogether delineated by a basement membrane and constituting ovarian cords. The formation of the definitive ovarian functional units requires their fragmentation into follicles formed by a single oocyte surrounded by granulosa cells and delineated by a basement membrane. This partitioning occurs within a tiny period and is dependent on the correct timing of the meiotic process, on an apoptotic wave that specifically targets oocytes and the remodelling of the basement membrane. Follicle formation is a crucial event of ovarian development because the follicle stock formed at the end of this process is non-renewable. Therefore, any disturbance of this process can lead to reproductive abnormalities in adulthood. Recent data on endocrine disruptors displaying estrogenic activity have involved the fragile estrogenic homeostasis in the process. We aimed at better understanding how the endogen estrogen 17b-estradiol (E2) contributes to follicle formation. To assess it, Sprague-Dawley rats were treated with E2 at different doses during follicle formation, /i.e./ in the 3 days following birth. We show that although this treatment impairs the development of the hepatic and ovarian detoxification systems, the female neonate is able to increase its estrogen clearance capabilities. Nevertheless, E2 treatment within this critical period triggers a dose-dependent decrease of oocyte number per ovary. Regardless of the E2 dose, puberty is advanced. All treated females are at least transiently fertile, yet these reproductive capabilities rapidly decline. A high (10 µg/day) dose of E2 leads to a secondary infertility characterized by anovulation that probably result from a dysfunction of the whole reproductive tract. By contrast, a lower (0.1 µg/day) dose of E2, that does not significantly modify neonatal oocyte survival, leads to a progressive reproductive senescence likely due to ovarian failure. Long-term troubles may originate from precocious E2 impact on the ovary since it disturbs ovarian transcriptome and produces many lesions on ovarian cell DNA.
L'ovaire est au cœur de la physiologie de la reproduction féminine. Il est à la fois à l'origine des ovocytes nécessaires à la fécondation et acteur des régulations endocrines du système reproducteur. Les ovocytes de l'ovaire fœtal sont groupés en amas bordés de cellules somatiques et d'une membrane basale, formant les cordons ovariens. Ces cordons ovariens se fragmentent pour former les follicules ovariens: un ovocyte bordé de cellules somatiques et d'une membrane basale. Cette fragmentation se déroule sur une courte période (/in utero/ chez la femme et juste après la naissance chez les rongeurs) et fait intervenir une vague de mort cellulaire programmée touchant les ovocytes pendant leur méiose et un remodelage de la membrane basale. La formation des follicules est une période cruciale du développement ovarien puisque le stock de follicules formés à l'issue de ce processus est non renouvelable. Son altération peut donc être à l'origine de troubles de la fonction de reproduction chez le futur adulte. Il existe une fragile homéostasie œstrogénique en place au moment de la formation folliculaire: chez la femme, l'ovaire se développe /in utero/, sous l'influence des œstrogènes circulants maternels et la production ovarienne fœtale. Chez les rongeurs, les follicules se forment lors de la levée de l'imprégnation hormonale maternelle au moment de la naissance. Nous avons souhaité comprendre comment le 17b-œstradiol (E2), œstrogène endogène, pouvait contribuer à la mise en place des follicules. Pour cela, des rattes Sprague-Dawley ont été traitées pendant la période de formation des follicules avec de l'E2 à différentes doses entre 0.01 et 10 µg/jour. Nos travaux démontrent que le traitement de rattes par des œstrogènes entre leur naissance et 3 jours induit une réduction dose-dépendante de leur nombre d'ovocytes. Bien que le traitement entrave le développement normal du système de détoxication hépatique et ovarien, l'animal est capable d'accroître ses capacités d'élimination des œstrogènes. Ces capacités sont toutefois insuffisantes pour bloquer les effets du traitement. Quelle que soit la dose d'E2 utilisée (0.1 ou 10 µg/jour), la puberté est plus précoce chez les femelles exposées mais toutes sont fertiles, au moins transitoirement. Cependant le traitement induit une dégradation rapide des capacités reproductives. L'E2 modifie la dynamique folliculaire chez l'adulte, longtemps après l'arrêt du traitement. Si une forte dose d'E2 réduit la survie des ovocytes lors de la formation des follicules et conduit à une infertilité secondaire due à un dysfonctionnement général du tractus reproducteur, une dose faible n'affecte pas la survie des ovocytes à court terme mais conduit à une sénescence reproductive précoce vraisemblablement d'origine ovarienne. L'origine de ces troubles pourrait résider dans les effets précoces de l'exposition à E2 : cette molécule altère le transcriptome ovarien et induit de nombreuses lésions de l'ADN des cellules ovariennes.
https://tel.archives-ouvertes.fr/tel-00953657

NNT : 2013REN1S107
tel-00953657
https://tel.archives-ouvertes.fr/tel-00953657
https://tel.archives-ouvertes.fr/tel-00953657/document
https://tel.archives-ouvertes.fr/tel-00953657/file/CHALMEY_Clementine.pdf

Éditeur(s) : HAL CCSD Wiley
Résumé : Clay shale landscapes are sensitive to landslide, erosion, and re-sedimentation processes. In this context, tectonized clay shales have peculiar hydrological and mechanical behaviours. In order to improve our understanding of the processes involved in such systems, an experimental site has been settled in the Draix Observatory (ORE DRAIX) on a black marl hill slope of the Southern Alps (France) in the framework of the ECOU-PREF project. The site is a natural laboratory to study the evolution of clay shale landscapes at several scales (regional to µm). In this study, we aim to characterize the internal structure and fissure patterns of a stable interfluve, in order to locate preferential water flows within the system, and anticipate its evolution. This work is based on a series of mutli-disciplinary approaches including petrophysical analyses, well pulse injection tests and downhole geophysical measurements. Borehole geophysical results were combined with laboratory measurements on core plugs (permeability, density, porosity, and acoustic velocity). Optical and acoustical images have been used as an original tool for internal discontinuities characterisation and potential active flow path detection. They allowed the identification of three main lithological units interpreted as several stages of shale alteration. In addition, several main structural plane discontinuities (open fractures, schistosity planes, and fractures infilled with calcite or clays) were identified from the images and their potential transmissivity discussed with regard to piezometric and tracer measurements. In all, the integration of measurements leads to propose a simple scenario of fluid circulation and chemical alteration of the interfluve.
ISSN: 0885-6087

hal-00757581
https://hal.archives-ouvertes.fr/hal-00757581
DOI : 10.1002/hyp.7997

Éditeur(s) : HAL CCSD Society for the Study of Reproduction
Résumé : International audience
Spermmotility notably depends on the structural integrity of the flagellum and the regulation ofmicrotubule dynamics. Although researchers have started to use " omics" techniques to characterize the human sperm's molecular landscape, the constituents responsible for the assembly, organization, and dynamics of the flagellum microtubule have yet to be fully defined. In this study, we defined a core set of 116 gene products associated with the human sperm microtubulome (including products potentially involved in abnormal ciliary phenotypes and male infertility disorders). To this end, we designed and applied an integrated genomics workflow and combined relevant proteomics, transcriptomics, and interactomics datasets to reconstruct a dynamic interactome map. By further integrating phenotypic information, we defined a disease-interaction network; this enabled us to highlight a number of novel factors potentially associated with altered sperm motility and male fertility. Lastly, we experimentally validated the expression pattern of two candidate genes (CUL3 and DCDC2C) that had never previously been associated with male germline differentiation. Our analysis suggested that CUL3 and DCDC2C's products have important roles in the sperm flagellum. Taken as a whole, our results demonstrate that an integrated genomics strategy can highlight relevant molecular factors in specific sperm components. This approach could be easily extended by including other " omics" data (from asthenozoospermic men, for example) and identifying other critical proteins from the human sperm microtubulome. Summary Sentence Although researchers have started to use "omics" techniques to characterize the human sperm's molecular landscape, the constituents responsible for the assembly, organization, and dynamics of the flagellum microtubule have yet to be fully defined.
ISSN: 0006-3363

hal-01515563
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01515563
DOI : 10.1095/biolreprod.116.143479
PUBMED : 28395323

Éditeur(s) : HAL CCSD
Résumé : Staphylococcus aureus is an opportunistic pathogen of humans and warm-blooded animals. It is foundin healthy carriage and can cause a wide range of infections in its hosts. The epithelial surface is theprincipal site of staphylococcal infection. The pathogens have evolved sophisticated mechanisms tohijack the main function of the host cells, thus promoting their invasion and colonization. Amongstthese effects are, alteration of host cell apoptosis, promotion of cell proliferation or conversely,inhibition of cell growth. Using human cervix cancer HeLa and mammary gland bovine MAC-Tepithelial cells, we studied the cytopathic effect of S. aureus and its ability to affect host cell cycle.Using microscopic examination, we found that S. aureus induced the cytopathic effect resulting in theenlargement of the host cells 72 hours post infection. Our experiments showed that the proliferationrate of the epithelial cells was slowed down in S. aureus-infected culture. Three different S. aureusstrains from human and animal origins were used and showed similar effect on both human and bovinecells. Microscopic analysis of DAPI-stained cells revealed huge decrease of mitotic indexes in infectedHeLa and Mac-T cells, synchronized by Double Thymidine Block. Using flow cytometry analysis, wefound that S. aureus induces G2/M phase delay in synchronous HeLa cells. By Western blot analysis,we showed that the G2/M phase delay was associated with accumulation of inactive phosphorylatedcyclin-dependent kinase Cdk1/cdc2, a key inducer of mitosis entry, and dephosphorylation of corehistone H3, an important component of the epigenetic landscape.These results suggest that the delay in G2/M phase transition of the infected epithelial cells may beone of the mechanisms employed by S. aureus to survive and to propagate inside the host. A fullcomprehension of the mechanisms associated to S. aureus-host cell interactions will undoubtedly helpto discover novel targets to treat staphylococcal infections.
EMBO
Villars sur Ollon, Switzerland

hal-01454214
https://hal.archives-ouvertes.fr/hal-01454214
PRODINRA : 171657

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The underlying cause of morbidity in cystic fibrosis (CF) is the decline in lung function, which results in part from chronic inflammation. Inflammation and infection occur early in infancy in CF and the role of innate immune defense in CF has been highlighted in the last years. Once thought simply to be consumers of bacteria, macrophages have emerged as highly sensitive immune cells that are located at the balance point between inflammation and resolution of this inflammation in CF pathophysiology. In order to assess the potential role of macrophage in CF, we review the evidence that: (1) CF macrophage has a dysregulated inflammatory phenotype; (2) CF macrophage presents altered phagocytosis capacity and bacterial killing; and (3) lipid disorders in CF macrophage affect its function. These alterations of macrophage weaken innate defense of CF patients and may be involved in CF disease progression and lung damage.
ISSN: 1569-1993

hal-01560280
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560280
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560280/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560280/file/Chouly-The%20impact%20of%20impaired%20macrophage%20functions.pdf
DOI : 10.1016/j.jcf.2016.10.011

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We present in this study the first data on Ra isotopes, 222Rn, and 210Pb in waters from the Lez karst system, one of the main ground-water resources in the South of France. The low Ra isotope activities of the Lez system waters (from 0.35 to 3.45 mBq/L of 226Ra) were measured by gamma spectrometry using the method developed in our previous work. Our data reveal the coherent behavior of 226Ra in Lez spring waters, well correlated to other major and trace elements, like Cl, Na, K, Mg, Li, Rb and Ba. The (228Ra/226Ra) ratios are distinct between the Lez spring (0.60 to 0.72) and two neighboring springs (0.52 to 0.65), showing the spatial variability in karst systems. In spite of their small variations in the Lez spring, it was possible to trace different water components and mixing processes from Lower-Cretaceous, Upper-Jurassic and Middle Jurassic end-members, in agreement with previous studies based on major and trace elements. Data obtained on waters derived from the Upper-Jurassic karst reveal that their (228Ra/226Ra) ratios are not related to the Th/U ratio of the Jurassic limestone, but are rather influenced by the soil and alterite cover of the recharge area.222Rn activities in the Lez spring are relatively low (0.5 to 3.7 Bq/L) compared to other karst regions. The highest 222Rn activities are found in diluted waters following heavy rainfall episodes, suggesting a subsurface origin of Rn. Soil and alterite samples that have much higher 226Ra activities (from 51 to 130 Bq/kg), compared to those from the main aquifer rocks (from 2.9 to 16 Bq/kg), are probably the main source of Rn, rather than the rocks of the karst itself. The study of several flood episodes reveals the correlation between Rn peak-values or total activity discharged at the spring with cumulative rainfall. Finally, 222Rn dissolved in water decays to 210Pb, and our data show that constraints on transfer times of underground waters can potentially be obtained from their coupled measurement.
ISSN: 0009-2541

hal-01622749
https://hal.archives-ouvertes.fr/hal-01622749
DOI : 10.1016/j.chemgeo.2017.06.022

Éditeur(s) : HAL CCSD Frontiers Media
Résumé : International audience
With global climate change altering marine ecosystems, research on plankton ecology is likely to navigate uncharted seas. Yet, a staggering wealth of new plankton observations, integrated with recent advances in marine ecosystem modelling, may shed light on marine ecosystem structure and functioning. A EuroMarine foresight workshop on the “Impact of climate change on the distribution of plankton functional and phylogenetic diversity” (PlankDiv) identified five grand challenges for future plankton diversity and macroecology research: 1) What can we learn about plankton communities from the new wealth of high-throughput ‘omics’ data? 2) What is the link between plankton diversity and ecosystem function? 3) How can species distribution models be adapted to represent plankton biogeography? 4) How will plankton biogeography be altered due to anthropogenic climate change? and 5) Can a new unifying theory of macroecology be developed based on plankton ecology studies? In this review, we discuss potential future avenues to address these questions, and challenges that need to be tackled along the way.
ISSN: 2296-7745

hal-01545318
https://hal.archives-ouvertes.fr/hal-01545318
DOI : 10.3389/fmars.2017.00068

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Leg ulcer is a disabling complication in patients with sickle cell anemia (SCA) but the exact pathophysiological mechanisms are unknown. The aim of this study was to identify the hematological and hemorheological alterations associated with recurrent leg ulcers. Sixty-two SCA patients who never experienced leg ulcers (ULC-) and 13 SCA patients with a positive history of recurrent leg ulcers (ULC+) - but with no leg ulcers at the time of the study - were recruited. All patients were in steady state condition. Blood was sampled to perform hematological, biochemical (hemolytic markers) and hemorheological analyses (blood viscosity, red blood cell deformability and aggregation properties). The hematocrit-to-viscosity ratio (HVR), which reflects the red blood cell oxygen transport efficiency, was calculated for each subject. Patients from the ULC+ group were older than patients from the ULC- group. Anemia (red blood cell count, hematocrit and hemoglobin levels) was more pronounced in the ULC+ group. Lactate dehydrogenase level was higher in the ULC+ group than in the ULC- group. Neither blood viscosity, nor RBC aggregation properties differed between the two groups. HVR was lower and RBC deformability tended to be reduced in the ULC+ group. Our study confirmed increased hemolytic rate and anemia in SCA patients with leg ulcers recurrence. Furthermore, our data suggest that although systemic blood viscosity is not a major factor involved in the pathophysiology of this complication, decreased red blood cell oxygen transport efficiency (i.e., low hematocrit/viscosity ratio) may play a role.
ISSN: 1932-6203

inserm-00922105
http://www.hal.inserm.fr/inserm-00922105
http://www.hal.inserm.fr/inserm-00922105/document
http://www.hal.inserm.fr/inserm-00922105/file/2013_Connes_-_Decreased_Hematocrit.pdf
DOI : 10.1371/journal.pone.0079680

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Timétrine massif exposed west of the Pan-African suture zone in northeastern Mali belongs to the passive margin of the West African craton facing to the east intra-oceanic arc assemblages and 730 Ma old pre-collisional calc-alkaline plutons. The Timétrine lithologic succession includes from the base to the top Mesoproterozoic cratonic to passive margin formations overlain by deep-sea Fe-Mg schists. Submarine metabasalts and two ultramafic massifs of serpentinized mantle peridotites are inserted as olistoliths towards the top whereas turbidites of continental origin represent the younger unit. Field and petrological data have revealed a distinct metasedimentary sequence attached to the serpentinized peridotites. It essentially consists of impure carbonates, Fe jaspers and polymictic breccias containing altered blocks of mantle peridotites, most rocks being enriched in detrital chromite. This association is interpreted as reworked chemical and detrital sediments derived from the alteration of mafic-ultramafic rocks. It is argued that mantle exhumation above sea floor took place during the Neoproterozoic rifting and crustal thinning period under possible tropical conditions, as suggested by the large volume of silicified serpentinites. In spite of greenschist facies metamorphic overprint characterized by widespread Fe-rich blue amphiboles that are not diagnostic of high-pressure conditions, it is possible to reconstruct a former ocean-continent transition similar to that evidenced for the Mesozoic period, followed by the deposition of syn-to post rift terrigeneous turbidites roughly coeval with ocean spreading some time before 800 Ma. It is concluded that the serpentinite massifs were tectonically emplaced first in an extensional setting, then incorporated within deep-sea sediments as olistoliths and finally transported westward during late Neoproterozoic collisional tectonics onto the West African craton.
ISSN: 0899-5362

hal-00985586
https://hal.archives-ouvertes.fr/hal-00985586
DOI : 10.1016/j.jafrearsci.2013.11.004

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The kinetics of hydrogenation of dry iron-bearing olivine single crystals was determined by performing hydration experiments under hydrothermal conditions at high pressure. The experiments were performed in a multi-anvil press at 3 GPa, for a temperature range between 900 and 1200 °C and for various durations. The oxygen fugacity was buffered along Ni-NiO joint. Polarized Fourier transform infrared spectroscopy and recent empirical calibration were used to quantify the hydroxyl distributions in the samples along crystallographic axes after the experiments. The chemical diffusion coefficients are similar (barely slower) than in olivine hydrated at lower pressure (0.2 and 0.3 GPa) for the same diffusion mechanism. Under the given experimental conditions, the anisotropy of diffusion is the same as for proton-vacancy mechanism, with diffusion along the [0 0 1] axis faster than along the [1 0 0]. However, the anisotropy at 3 GPa is weaker compared to measurements at lower pressures and the analysis of concentration profiles using 3D models shows that an isotropic solution could also be relevant. Fits of the diffusion data to an Arrhenius law yield activation energies for the slightly faster [0 0 1] axis of the crystallographic axes around 198 ± 5 kJ mol−1, a value only slightly lower than the results from previous experimental studies for natural iron-bearing olivine hydrogenated at lower confining pressure. At 3 GPa, hydrogenation can be well approximated by a single mechanism controlled by coupled diffusion of protons and octahedral vacancies (di- and tri-valent ions). The diffusion rates are fast enough to alter hydrogen concentration within olivine in xenoliths ascending from the mantle or experiencing hydrogen-rich metasomatism events, but too slow to permit complete homogenization of hydrogen in olivine-rich rocks at kilometer scale in less than one My.
ISSN: 0031-9201

hal-01412663
https://hal.archives-ouvertes.fr/hal-01412663
DOI : 10.1016/j.pepi.2016.08.005

Éditeur(s) : HAL CCSD
Résumé : International audience
Tin mineralization in Achmmach deposit (NE of Moroccan Central Massif) is associated with tourmaline-rich alteration halos and faults, within N70 shear zones, hosted in sandstones and metapelites of the Upper Visean-Namurian. Such deposits were reported as late-Variscan in age and related to the emplacement of late orogenic granite not outcropping in the studied area.From field constraints, it appears that the late Variscan phase is marked by a transition from transpression to extension with conditions of deformation evolving from ductile to brittle environments. During the transpressive phase (horizontal shortening direction roughly trending E-W), pervasive tourmaline is deposited under the form of metasomatic halos around fractures in the upper levels relative to the deep batholith. This hydrothermal activity is coeval with ductile shear zones within a large corridors trending N70. Tourmaline-rich alteration appears as a hardening stage contributing to the modification of the tectonic style of rocks. Thereafter, a strong brecciation occurs in response of re-using and re-opening of the strike-slip structure. The result is (1) the formation of dextral (N070, N020) and sinistral (N120) shears, and (2) the circulation of fluids within early tourmalinites and formation of tourmaline-rich breccia. Subsequently, during the late orogenic extensional phase, tourmalinite breccias reopened as normal faults acting as overpressure fluid pathways for developing other breccias and veins. Such event is marked by deposition of tin mineralization i.e., cassiterite followed by sulphides, and then fluorite and carbonates at the end.From analysis of the shear-zone structure and superimposition of tourmalines and tin mineralization in the same structures, it is possible to suggest a structural control continuum. Such control is well expressed during metasomatism and deposition of hydrothermal tourmaline under the transpressive phase. Thereafter, under the extensive phase allowing open-space, tin-mineralization were trapped.This study has been supported by AI MA/09/210
24ème RST
Pau, France

insu-01546927
https://hal-insu.archives-ouvertes.fr/insu-01546927

Éditeur(s) : HAL CCSD Tecpar
Résumé : International audience
The general structure and organization of the shallow water (<1.5 m) fish assemblages were studied in two southern Brazilian ecosystems, a bay and a lagoon, under different marine or continental influences. The abiotic factors were measured to define the hydrology of the sites and the biotic descriptors were evaluated to characterize the assemblage structures. Transparency, salinity and organic matter were the abiotic factors which best distinguished the sites. A total richness of 69 species was observed. Ecological guilds, density, diversity and evenness indices values were different in each site. Hydrodynamism and geomorphology determined the structure and the organization of the fish assemblages in these shallow waters. It was observed that variations in the hydrological attributes generated by the marine or continental water movements are able to alter the conditions in the lagoon more quickly and at more regular frequencies than those in the bay.
ISSN: 1516-8913

hal-00753018
https://hal.univ-antilles.fr/hal-00753018
https://hal.univ-antilles.fr/hal-00753018/document
https://hal.univ-antilles.fr/hal-00753018/file/BRAZILIAN_2010.pdf

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
BACKGROUND: Phthalic acid esters are widely used in the manufacture of plastics. Numerous studies have shown that these phthalates impair testicular testosterone production in the rat. However, the scarce and contradictory data concerning humans have cast doubt over whether these compounds are also anti-androgenic in man. We therefore investigated the direct effects of di-(2-ethylhexyl) phthalate (DEHP) and mono-(2-ethylhexyl) phthalate (MEHP) on organo-cultured adult human testis and a human cell line. METHODS: Adult human testis explants or NCI-H295R adrenocortical human cells were cultured with DEHP or MEHP. The effects of ketoconazole, used as a reference molecule, were also assessed. RESULTS: In both models, DEHP and MEHP significantly inhibited testosterone production. The effects of both phthalates appeared to be specific for steroidogenesis, as INSL3 production by Leydig cells was not altered. Furthermore, the phthalates of interest had no effect on inhibin B production by Sertoli cells or on germ cell apoptosis. As only a small fraction of the phthalates added was found in the testis explants, and as these compounds were found to be metabolized, we estimate that the anti-androgenic effects observed occurred at concentrations of phthalates that are of the same order of magnitude as exposures reported in the literature for men. CONCLUSIONS: We provide the first evidence that DEHP and MEHP can inhibit testosterone production in the adult human testis. This is consistent with recent epidemiological findings of an inverse correlation between exposure to MEHP and testosterone concentrations.
ISSN: 0268-1161

hal-00876965
https://hal.archives-ouvertes.fr/hal-00876965
DOI : 10.1093/humrep/des069
PUBMED : 22402212

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : Functional aspects of biodiversity were investigated in a lowland tropical rainforest in French Guyana (5°2'N, annual precipitation 2200 mm). We assessed leaf '15N as a presumptive indicator of symbiotic N2 fixation, and leaf and wood cellulose '13C as an indicator of leaf intrinsic water-use efficiency (CO2 assimilation rate/leaf conductance for water vapour) in dominant trees of 21 species selected for their representativeness in the forest cover, their ecological strategy (pioneers or late successional stage species, shade tolerance) or their potential ability for N2 fixation. Similar measurements were made in trees of native species growing in a nearby plantation after severe perturbation (clear cutting, mechanical soil disturbance). Bulk soil '15N was spatially quite uniform in the forest (range 3-5‰), whereas average leaf '15N ranged from m0.3‰ to 3.5‰ in the different species. Three species only, Diplotropis purpurea, Recordoxylon speciosum (Fabaceae), and Sclerolobium melinonii (Caesalpiniaceae), had root bacterial nodules, which was also associated with leaf N concentrations higher than 20 mg gm1. Although nodulated trees displayed significantly lower leaf '15N values than non-nodulated trees, leaf '15N did not prove a straightforward indicator of symbiotic fixation, since there was a clear overlap of '15N values for nodulated and non-nodulated species at the lower end of the '15N range. Perturbation did not markedly affect the difference '15Nsoil m '15Nleaf, and thus the isotopic data provide no evidence of an alteration in the different N acquisition patterns. Extremely large interspecific differences in sunlit leaf '13C were observed in the forest (average values from m31.4 to m26.7‰), corresponding to intrinsic water-use efficiencies (ratio CO2 assimilation rate/leaf conductance for water vapour) varying over a threefold range. Wood cellulose '13C was positively related to total leaf '13C, the former values being 2-3‰ higher than the latter ones. Leaf '13C was not related to leaf '15N at either intraspecific or interspecific levels. '13C of sunlit leaves was highest in shade hemitolerant emergent species and was lower in heliophilic, but also in shade-tolerant species. For a given species, leaf '13C did not differ between the pristine forest and the disturbed plantation conditions. Our results are not in accord with the concept of existence of functional types of species characterized by common suites of traits underlying niche differentiation; rather, they support the hypothesis that each trait leads to a separate grouping of species.
ISSN: 0029-8549

hal-01032378
https://hal.archives-ouvertes.fr/hal-01032378
DOI : 10.1007/s004420050593

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
Progress in seismic methodology and ambitious large-scale seismic projects are enabling high-resolution imaging of the continental crust. The ability to constrain interpretations of crustal seismic data is based on laboratory measurements on rock samples and calculations of seismic properties. Seismic velocity calculations and their directional dependence are based on the rock microfabric, which consists of mineral aggregate properties including crystallographic preferred orientation (CPO), grain shape and distribution, grain boundary distribution, and misorientation within grains. Single-mineral elastic constants and density are crucial for predicting seismic velocities, preferably at conditions that span the crust. However, high-temperature and high-pressure elastic constant data are not as common as those determined at standard temperature and pressure (STP; atmospheric conditions). Continental crust has a very diverse mineral composition; however, a select number of minerals appear to dominate seismic properties because of their high-volume fraction contribution. Calculations of microfabric-based seismic properties and anisotropy are performed with averaging methods that in their simplest form takes into account the CPO and modal mineral composition, and corresponding single crystal elastic constants. More complex methods can take into account other microstructural characteristics, including the grain shape and distribution of mineral grains and cracks and pores. Dynamic or active wave propagation schemes have recently been developed, which offer a complementary method to existing static averaging methods generally based on the use of the Christoffel equation. A challenge for the geophysics and rock physics communities is the separation of intrinsic factors affecting seismic anisotropy, due to properties of crystals within a rock and apparent sources due to extrinsic factors like cracks, fractures, and alteration. This is of particular importance when trying to deduce crustal composition and the state of deformation from seismic parameters.
ISSN: 8755-1209

Droits : info:eu-repo/semantics/OpenAccess
hal-01685568
https://hal.archives-ouvertes.fr/hal-01685568
https://hal.archives-ouvertes.fr/hal-01685568/document
https://hal.archives-ouvertes.fr/hal-01685568/file/Almqvist_et_al-2017-Reviewssur Geophysics.pdf
DOI : 10.1002/2016RG000552

Éditeur(s) : HAL CCSD IOS Press
Résumé : International audience
Because of the metabolic changes induced by a physical activity, the hemorheological properties of patients with sickle cell anemia could be further impaired and increase the risks for vaso-occlusive complications. However, few studies suggest that moderate physical activity could be beneficial rather than harmful in patients with sickle cell anemia (SCA). However, the definition of what can be considered as a moderate physical activity in SCA patients is imprecise. The present study tested the effects of a short incremental cycling exercise test conducted until the first ventilatory threshold on different biomarkers. Hematological and hemorheological parameters were compared between 8 patients with SCA and 13 healthy subjects (CONT) before, immediately after the end of the exercise and at 12, 36 and 60 hours after the exercise. We observed no significant hematological or hemorheological alteration induced by the exercise in the two groups. However, the exercise resulted in a delayed improvement of the red blood cell disaggregation threshold at 36 and 60 hrs after exercise in the SCA group which was paralleled to the decrease in the platelet count in this group. The present study suggests that such an exercise might be beneficial for microcirculatory blood flow.
ISSN: 1386-0291

hal-01137567
https://hal.univ-antilles.fr/hal-01137567

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Este trabajo indaga en la forma de componer la apariencia femenina hacia el inicio del siglo XX, como signo altamente valorado de un estilo de vida. Se hará hincapié en la moda para el ocio en la playa. En el marco de una práctica insipiente, se priorizó la necesidad de exaltar la distinción por sobre la vivencia de las sensaciones que podrían provocar el contacto con la naturaleza. Se entiende que para esto fueron decisivas las pautas tipológicas, tecnológicas y funcionales del vestir. Los atributos como forma, materialidad, modos de confección y de uso trascendieron su rol técnico para volverse condicionantes de las formas de comportamiento e interacción social y para comunicar los modos que podía asumir el gusto por el lujo.
Ce travail de recherche est consacré à la construction de l'apparence féminine, au début du xxe siècle, comme style de vie jouissant d’une grande considération. L’accent est mis sur le développement de la mode sur les plages, dans un cadre d’oisiveté. Dans le cadre de l’émergence de ces pratiques naïves, la priorité fut l’exaltation d’un besoin de distinction plutôt que la recherche de sensations au contact de la nature. De nouvelles normes émergent en combinant les types, les technologies et la fonctionnalité de l’habillement. Les attributs comme la forme, la matérialité, la confection et l’usage constituent des éléments déterminants dans les formes de comportement et les interactions sociales et ils illustrent les différentes manières d’assumer le goût du luxe.
This work investigates in the way of composing the feminine appearance to the beginning of the 20th century, as sign highly valued of a way of life. It will be emphasized in the mode used for the leisure in the beach. In the frame of an ignorant practice, there was prioritized the need to exalt the distinction for on the experience of the sensations that might provoke the contact with the nature. There is understood that for it were decisive the guidelines typological, technological and functional of to dress. The attributes like form, materiality, confection and use, came out his technical role to become determining of the forms of behavior and social interaction and to communicate the manners that the taste could assume for the luxury.

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.7348
http://journals.openedition.org/etudescaribeennes/7348