Éditeur(s) : Gauthier-Villars
Résumé : Calcareous nannofossil assemblages of the family Noelaerhabdaceae have been studied in sediments of seventeen Quaternary cores raised from various climatic areas of the Atlantic and Pacific oceans. These sections display a regular, hiatus-free sedimentation for the last 500000 years. They have been dated using oxygen-isotopic measurements, variations of calcium carbonate content, variations of total nannofossil abundance, thorium measurements and volcanic ash horizons. Coccoliths of the genus Gephyrocapsa have been placed in three distinct taxonomic groups, based on morphological criteria inferred from light-microscope observations. Their acmes, as well as the acme of Emiliania huxleyi, are considered as stratigraphic markers. These markers characterize most of the last thirteen glacial-interglacial climatic stages, as well as some climatic events of lower magnitude (8.5 and 6.5). Most of these markers have global stratigraphic significance: acme of ''closed'' Gephyrocapsa during stages 13,11 and 9, and during event 8.5; acme of ''small'' Gephyrocapsa during stage 7 and event 6.5; acme of ''open'' Gephyrocapsa at top of event 6.5, and during stages 5 and 3; acme of E. huxleyi during stages 3 and 1. Others are only valid south of the southernmost latitude reached by the North-Atlantic polar front during the Quaternary: acme of ''closed'' Gephyrocapsa during stage 12; acme of ''small'' Gepkyrocapsa in early and middle stage 5; acme of E. huxleyi during stage 2. Others are only valid south of 20-degrees-S: acme of ''small'' Gephyrocapsa at the 10/9 stage boundary; acme of ''open'' Gephyrocapsa during stage 6.
Oceanologica Acta (0399-1784) (Gauthier-Villars), 1993 , Vol. 16 , N. 4 , P. 349-362

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00099/21061/18687.pdf
http://archimer.ifremer.fr/doc/00099/21061/

Éditeur(s) : Elsevier Science Bv
Résumé : In New Caledonia, shrimp hatcheries are confronted with mass mortality in the larval stages, a phenomenon poorly understood as no specific causative agent has been identified. This has resulted in an excessive use of prophylactic antibiotics, although their adverse effects in aquaculture are notorious. The present work was thus aimed at selecting potential probiotic strains for penaeid hatcheries. From a pool of more than 400 marine bacterial isolates sampled from the local marine environment seven strains exhibited in vitro antagonistic activity towards Vibrio harveyi. These isolates were characterized both phenotypically and genotypically using a biochemical approach and 16S rDNA sequencing. Six out of these seven strains were found to belong to the genus Pseudoalteromonas, the last one belonging to the Vibrionaceae family and related to the Harveyi clade. Selected probiotic candidates were individually tested for antagonistic activities in vitro using a green fluorescent protein (GFP)-labelled transconjugant of V. harveyi and for inherent pathogenicity towards cultured shrimp larvae at two different developmental stages. This approach enabled rapid processing and selection of candidates to be tested in our experimental hatchery. Four different experiments were conducted to test candidate strains either alone or in combination. Repeated trials showed that postlarval survival was significantly improved by adding the strain NC201 individually to the rearing water compared to unchallenged controls. The analysis of immune-related gene expressions showed that the Litsty PEN3 transcript abundance of larvae was significantly increased after being reared in probiotic-containing water; however no significant difference in lysozyme gene expression was recorded in this study. Together, these results open new insights into the use of these strains as potential substitutes to antibiotherapy in shrimp larval rearing in New Caledonia.
Aquaculture (0044-8486) (Elsevier Science Bv), 2014-08 , Vol. 432 , P. 475-482

Droits : 2014 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/00190/30129/28591.pdf
DOI:10.1016/j.aquaculture.2014.04.031
http://archimer.ifremer.fr/doc/00190/30129/

Éditeur(s) : HAL CCSD
Résumé : International audience
Yellow leaf disease is a recent, important and widely spread disease of sugarcane (6), causing severe yield losses on susceptible cultivars. The disease is caused by Sugarcane Yellow Leaf Virus (SCLRV) of the genus Polerovirus in the family Luteoviridae. This virus is transmitted from plant to plant only by aphids in a persistent, circulative and non replicative manner. The epidemic of the SCYLV was little studied and there is a need for information on the spatial spread of the disease and on the role of the aphid in the disease spread. SCYLV was first identified in Guadeloupe in 1996 and Melanaphis sacchari has been the only SCYLV vector found in sugarcane fields. Even if the infection rate is relatively low (0% to 21%), disease may increase and cause damage to sugarcane in Guadeloupe. Even though disease free tissue cultured plants are used for sanitary control in Guadeloupe, understanding SCYLV spread by its vector may help determine additional management strategies against the virus.
IEW
New York, United States

hal-00684226
https://hal.archives-ouvertes.fr/hal-00684226

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Defining homologous genes is important in many evolutionary studies but raises obvious issues. Some of these issues are conceptual and stem from our assumptions of how a gene evolves, others are practical, and depend on the algorithmic decisions implemented in existing software. Therefore, to make progress in the study of homology, both ontological and epistemological questions must be considered. In particular, defining homologous genes cannot be solely addressed under the classic assumptions of strong tree thinking, according to which genes evolve in a strictly tree-like fashion of vertical descent and divergence and the problems of homology detection are primarily methodological. Gene homology could also be considered under a different perspective where genes evolve as ``public goods,'' subjected to various introgressive processes. In this latter case, defining homologous genes becomes a matter of designing models suited to the actual complexity of the data and how such complexity arises, rather than trying to fit genetic data to some a priori tree-like evolutionary model, a practice that inevitably results in the loss of much information. Here we show how important aspects of the problems raised by homology detection methods can be overcome when even more fundamental roots of these problems are addressed by analyzing public goods thinking evolutionary processes through which genes have frequently originated. This kind of thinking acknowledges distinct types of homologs, characterized by distinct patterns, in phylogenetic and nonphylogenetic unrooted or multirooted networks. In addition, we define `` family resemblances'' to include genes that are related through intermediate relatives, thereby placing notions of homology in the broader context of evolutionary relationships. We conclude by presenting some payoffs of adopting such a pluralistic account of homology and family relationship, which expands the scope of evolutionary analyses beyond the traditional, yet relatively narrow focus allowed by a strong tree-thinking view on gene evolution.
ISSN: 0737-4038

hal-01544794
https://hal.archives-ouvertes.fr/hal-01544794
DOI : 10.1093/molbev/mst228

Éditeur(s) : HAL CCSD
Résumé : Communication between tumoral cells and the microenvironnement plays an essential role in the developpement of tumors. In that context, the new family of metalloproteases, the ADAM proteins, are major regulators of the tumoral progression by acting on the bioavaibility of importants mediators of cellular communication as cytokines and growth factors. Among this family, ADAM12 is the most associated with cancer. It has been shown to mediate signaling pathways by a process independant of its metalloproteasis activity, in particular for TGFβ signaling. This study show for the first time a role for the membrane form of ADAM12 in the induction of epithelial to mesenchymal transition (EMT), a essential process involved in tumor invasion, whom TGFβ is a main inducer. This effect is mediated by the activation of TGFβ signaling pathways, SMAD3, AKT and ERK and require the cytoplasmic tail of ADAM12L but not its catalytic activity. Activation of these pathways could involve a relocalisation of ADAM12L in special signaling platform in lipid rafts. Moreover, we have shown that ADAM12L interact with ZO-1 and ZO-2, two proteins of tight junctions, and could facilitate their desassembling during EMT. This work underscore for the first time a new function of ADAM12L in EMT, by a mecanism invovlving a modulation of signals regulating this process. A better understanding of the dynamic of these molecular mecanisms could allow the developpement of new targeted therapies to fight against tumoral progression.
Les échanges entre les cellules tumorales et le microenvironnement jouent un rôle essentiel dans le développement des tumeurs. Dans ce contexte, la nouvelle famille de métalloprotéases, les protéines ADAM, constituent aujourd’hui des régulateurs majeurs de la progression tumorale en agissant sur la biodisponibilité des médiateurs de la communication cellulaire que sont les cytokines, chimiokines et facteurs de croissance. Au sein de cette famille, ADAM12 est la plus associée au cancer. Elle possède la particularité de jouer un rôle dans la signalisation cellulaire, de façon indépendante de son activité métalloprotéase, notamment dans les voies de signalisation du TGFβ. Notre étude montre pour la première fois un rôle pour la forme membranaire d'ADAM12 dans l'induction de la transition épithélio-mésenchymateuse (EMT), un processus essentiel à l'invasion tumorale dont le TGFβ est un inducteur majeur. Cet effet est médié par l'activation des voies de signalisation du TGFβ, impliquant les protéines SMAD3, AKT et ERK et requiert le domaine cytoplasmique d'ADAM12L mais pas son domaine catalytique. L'activation de ces voies de signalisation pourrait impliquer une relocalisation d'ADAM12L au sein de plates-formes de signalisation dans les radeaux lipidiques. Par ailleurs, nous avons montré qu'ADAM12L interagit avec les protéines ZO-1 et ZO-2, des protéines des jonctions serrées, et pourrait favoriser leur désassemblage au cours de l'EMT. Nos travaux ont permis de mettre en évidence une nouvelle fonction pour ADAM12L dans l'EMT, par un mécanisme impliquant une modulation des signaux régulant ce processus. Une meilleure compréhension de la dynamique de ces mécanismes moléculaires pourrait permettre de développer de nouvelles thérapies ciblées pour lutter contre la progression tumorale.
https://tel.archives-ouvertes.fr/tel-01267577

Droits : info:eu-repo/semantics/OpenAccess
NNT : 2015REN1S063
tel-01267577
https://tel.archives-ouvertes.fr/tel-01267577
https://tel.archives-ouvertes.fr/tel-01267577/document
https://tel.archives-ouvertes.fr/tel-01267577/file/RUFF_Michael.pdf

Éditeur(s) : HAL CCSD Elsevier Elsevier Masson
Résumé : International audience
Prostate cancer is the most frequent cancer affecting men in most Western countries. Certain risk factors have been identified (age, family history, ethnic origin), but the aetiology of this cancer remains largely unknown. However, a role for environmental factors is strongly suspected. Questions have been raised concerning the role of the chemical substances generated by human activities in the occurrence of this disease. Diverse studies have consistently demonstrated a higher risk of prostate cancer in agricultural populations than in the general population. The hypothesis that this higher risk is linked to the use of pesticides has been tested in a number of studies, mostly in North America and Europe. However, to date, with a few possible exceptions, it has been impossible to demonstrate a significant association between exposure to pesticides or a chemical family of pesticides and prostate cancer. Studies have also been carried out on the role of exposure to trace metals, such as cadmium, or to pollutants from industry, such as polychlorobiphenyls. However, no firm conclusions have been drawn. Finally, the effect of chemical substances with endocrine disruptor activity on the occurrence of prostate cancer remains largely unexplored in epidemiological studies. In the face of these uncertainties, rigorous studies are required, with objective measurements of exposure, taking into account confounding factors and individual risk factors, making it possible to assess gene-environment interactions.
ISSN: 1297-9589

inserm-00524579
http://www.hal.inserm.fr/inserm-00524579
DOI : 10.1016/j.gyobfe.2008.07.005
PUBMED : 18693059

Éditeur(s) : HAL CCSD
Résumé : Enamel formation requires the involvement of secretory calcium-binding phosphoproteins. Three of them, amelogenin (AMEL), ameloblastin (AMBN) and enamelin (ENAM), have been extensively studied in mammals. More recently, a fourth protein belonging to the same family has been identified: amelotin (AMTN). AMTN has only been studied in rodents and its role during amelogenesis remains unclear.The aim of this thesis is to extend the knowledge on AMTN by studying its evolution in amniotes and its expression in the lizard Anolis carolinensis.AMTN sequences from many amniote species have been extracted from databases or obtained by PCR, and analyzed. Those analyses allowed us to reveal differences in the gene structure and to highlight residues that were conserved during mammalian or amniote evolution. These conserved amino acids are essential to the structure and/or function of the protein.The expression of AMTN and of AMEL, AMBN and ENAM has been studied by in situ hybridization on lizard jaw sections. Comparison of the expression pattern of these genes during amelogenesis in the lizard with that described in rodents points to similarities (AMEL, AMBN and ENAM) but also to important differences, especially in the spatio-temporal expression of AMTN. In order to better understand AMTN evolution in tetrapods, I studied its expression in an amphibian (Pleurodeles waltl) and a marsupial (Monodelphis domestica).Taken together, these results suggest a link between the evolution of AMTN gene structure (loss of exons and of functional domains in mammals) and its expression (early in non-mammals versus late in mammals) with the emergence of prismatic structure of enamel in early mammals.
La formation de l'émail dentaire met en jeu des phosphoprotéines sécrétées liant le calcium. Parmi celles-ci figurent amélogénine (AMEL), améloblastine (AMBN) et énaméline (ENAM), qui ont fait l'objet de nombreuses études chez les mammifères. Plus récemment, une quatrième protéine de la même famille a été découverte, l'amélotine (AMTN). Elle n'a été étudiée que chez les rongeurs et son rôle au cours de l'amélogenèse reste mal défini. L'objectif de cette thèse est d'élargir les connaissances sur AMTN en étudiant son évolution chez les amniotes et son expression au cours de l'amélogenèse chez le lézard Anolis carolinensis. Les séquences d'AMTN d'un grand nombre d'amniotes ont été extraites des bases de données ou obtenues par PCR, et analysées. Ces analyses ont mis en évidence différentes structures géniques et des acides aminés très conservés au cours de l'évolution. Cette conservation indique qu'ils sont essentiels à la structure ou à la fonction de la protéine. L'expression d'AMTN ainsi que celle d'AMEL, AMBN et ENAM ont été étudiées par hybridation in situ sur des coupes de mâchoires de lézard. La comparaison des patrons d'expression chez le lézard avec ceux décrits chez la souris a révélé des différences dans l'expression spatio-temporelle d'AMTN. De ce fait, afin de mieux comprendre l'évolution de ce gène, son expression a été étudiée chez un amphibien (Pleurodeles waltl) et un marsupial (Monodelphis domestica).Ces travaux suggère un lien entre l'évolution de la structure génique d'AMTN (perte d'exons et de domaines fonctionnels) et de son expression (précoce versus tardive) avec l'émergence de la structure prismatique de l'émail chez les mammifères.
https://tel.archives-ouvertes.fr/tel-01154518

NNT : 2015PA066066
tel-01154518
https://tel.archives-ouvertes.fr/tel-01154518
https://tel.archives-ouvertes.fr/tel-01154518/document
https://tel.archives-ouvertes.fr/tel-01154518/file/2015PA066066.pdf

Éditeur(s) : HAL CCSD Taylor & Francis (Routledge): STM, Behavioural Science and Public Health Titles
Résumé : International audience
The aim of this study was to determine factors associated with complete satisfaction with the care provided (satisfaction with physicians and satisfaction with services and organization) among HIV-infected patients followed up in the French ANRS CO8 APROCO-COPILOTE cohort. Analyses focused on cross-sectional data collected during the ninth year of cohort follow-up. Satisfaction with care, sociodemographic characteristics, and behavioral data were collected using self-administered questionnaires, while clinical data were derived from medical records. Complete satisfaction with care was defined as being 100% satisfied. Two logistic regression models were used to identify predictors of (1) complete satisfaction with physicians (n=404) and (2) complete satisfaction with services and organization (n=396). Sixteen percent of patients were completely satisfied with physicians, while 15.9% were completely satisfied with services and organization. Being older and reporting fewer discomforting antiretroviral therapy (ART) side effects were factors independently associated with complete satisfaction with both physicians and services and organization. Strong support from friends and absence of hepatitis C (HCV) co-infection were independently associated with complete satisfaction with physicians, while strong support from one's family and comfortable housing conditions were independently associated with complete satisfaction with services and organization. Even after nine years of follow-up, social vulnerabilities still strongly influence HIV-infected patients' interactions with the health care system. Day-to-day experience with the disease, including perceived treatment side effects, appears to play a key role in the quality of these interactions. More attention should be given to patient satisfaction, especially for socially vulnerable patients, in order to avoid potentially detrimental consequences such as poor adherence to ART.
AIDS Care

hal-00875619
https://hal.archives-ouvertes.fr/hal-00875619
DOI : 10.1080/09540121.2011.613909
PUBMED : 21999644

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : En dehors de l’école et de la famille, d’autres lieux permettent aux jeunes d’apprendre au contact des adultes : c’est ce qu’on appelle les tiers-lieux éducatifs. Les églises de la communauté haïtienne de la Martinique relèvent selon nous de cette catégorie. En effet, cette diaspora se retrouve dans des ecclésioles — véritables entreprises à la tête desquelles se trouvent des pasteurs (autoproclamés) issus de la communauté exilée — qui prennent en charge sa progéniture et l’aident à s’intégrer dans la société martiniquaise. Dans cet article, nous entendons démontrer cette fonction éducative de ces lieux de rassemblement de la communauté haïtiano-martiniquaise. À l’instar d’autres communautés évangéliques autochtones nous avons posé cette hypothèse explicative que nous avons vérifiée. Une fonction que ne rempliraient pas en revanche les rares temples vaudous de l’île à la tête desquels se trouvent des fils de Toussaint Louverture.
Outside regular school and family structures, the youth are exposed to other environments that allow them to learn from adults. This is referred to as “tiers-lieu éducatif” or "third-place education". Churches in Haitian communities in Martinique emerge, in our opinion, from this experience. In fact, the Haitian Diaspora is concentrated in areas that contain small ecclesiastical strongholds, enterprises led by self-acclaimed pastors who originate from the exiled community, assuming responsibility for their progeny, and thus helping them to integrate in the society. This article highlights the function of Education in these areas highly populated by Haitians. Following the example of the native Evangelical Communities, we have formulated the explanatory hypothesis that we have verified: A function that does not fulfil, on the other hand, the few voodoo temples on the island, led by the sons of Toussaint Louverture. 
Martinique

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.4600
http://journals.openedition.org/etudescaribeennes/4600

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: Myosin II (or Myosin Heavy Chain II, MHCII) is a family of molecular motors involved in the contractile activity of animal muscle cells but also in various other cellular processes in non-muscle cells. Previous phylogenetic analyses of bilaterian MHCII genes identified two main clades associated respectively with smooth/non-muscle cells (MHCIIa) and striated muscle cells (MHCIIb). Muscle cells are generally thought to have originated only once in ancient animal history, and decisive insights about their early evolution are expected to come from expression studies of Myosin II genes in the two non-bilaterian phyla that possess muscles, the Cnidaria and Ctenophora. Results: We have uncovered three MHCII paralogues in the ctenophore species Pleurobrachia pileus. Phylogenetic analyses indicate that the MHCIIa /MHCIIb duplication is more ancient than the divergence between extant metazoan lineages. The ctenophore MHCIIa gene (PpiMHCIIa) has an expression pattern akin to that of ``stem cell markers'' (Piwi, Vasa...) and is expressed in proliferating cells. We identified two MHCIIb genes that originated from a ctenophore-specific duplication. PpiMHCIIb1 represents the exclusively muscular form of myosin II in ctenophore, while PpiMHCIIb2 is expressed in non-muscle cells of various types. In parallel, our phalloidin staining and TEM observations highlight the structural complexity of ctenophore musculature and emphasize the experimental interest of the ctenophore tentacle root, in which myogenesis is spatially ordered and strikingly similar to striated muscle formation in vertebrates. Conclusion: MHCIIa expression in putative stem cells/proliferating cells probably represents an ancestral trait, while specific involvement of some MHCIIa genes in smooth muscle fibres is a uniquely derived feature of the vertebrates. That one ctenophore MHCIIb paralogue (PpiMHCIIb2) has retained MHCIIa-like expression features furthermore suggests that muscular expression of the other paralogue, PpiMHCIIb1, was the result of neofunctionalisation within the ctenophore lineage, making independent origin of ctenophore muscle cells a likely option.
ISSN: 1471-2148

hal-01544486
https://hal.archives-ouvertes.fr/hal-01544486
DOI : 10.1186/1471-2148-12-107

Éditeur(s) : HAL CCSD Frontiers Media
Résumé : International audience
To date 142 species have been described in the Vibrionaceae family of bacteria, classified into seven genera; Aliivibrio, Echinimonas, Enterovibrio, Grimontia, Photobacterium, Salinivibrio and Vibrio. As vibrios are widespread in marine environments and show versatile metabolisms and ecologies, these bacteria are recognized as one of the most diverse and important marine heterotrophic bacterial groups for elucidating the correlation between genome evolution and ecological adaptation. However, on the basis of 16S rRNA gene phylogeny, we could not find any robust monophyletic lineages in any of the known genera. We needed further attempts to reconstruct their evolutionary history based on multilocus sequence analysis (MLSA) and/or genome wide taxonomy of all the recognized species groups. In our previous report in 2007, we conducted the first broad multilocus sequence analysis (MLSA) to infer the evolutionary history of vibrios using nine housekeeping genes (the 16S rRNA gene, gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA), and we proposed 14 distinct clades in 58 species of Vibrionaceae. Due to the difficulty of designing universal primers that can amplify the genes for MLSA in every Vibrionaceae species, some clades had yet to be defined. In this study, we present a better picture of an updated molecular phylogeny for 86 described vibrio species and 10 genome sequenced Vibrionaceae strains, using 8 housekeeping gene sequences. This new study places special emphasis on (1) eight newly identified clades (Damselae, Mediterranei, Pectenicida, Phosphoreum, Profundum, Porteresiae, Rosenbergii, and Rumoiensis); (2) clades amended since the 2007 proposal with recently described new species; (3) orphan clades of genomospecies F6 and F10; (4) phylogenetic positions defined in 3 genome-sequenced strains (N418, EX25, and EJY3); and (5) description of V. tritonius sp. nov., which is a member of the “Porteresiae” clade.
ISSN: 1664-302X

hal-01546980
http://hal.upmc.fr/hal-01546980
DOI : 10.3389/fmicb.2013.00414

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Chaperone synthesis in response to proteotoxic stress is dependent on a family of transcription factors named heat shock factors (HSFs). The two main factors in this family, HSF1 and HSF2, are co-expressed in numerous tissues where they can interact and form heterotrimers in response to proteasome inhibition. HSF1 and HSF2 exhibit two alternative splicing isoforms, called α and β, which contribute to additional complexity in HSF transcriptional regulation, but remain poorly examined in the literature. In this work, we studied the transcriptional activity of HSF1 and HSF2 splicing isoforms transfected into immortalized Mouse Embryonic Fibroblasts (iMEFs) deleted for both Hsf1 and Hsf2, under normal conditions and after proteasome inhibition. We found that HSF1α is significantly more active than the β isoform after exposure to the proteasome inhibitor MG132. Furthermore, we clearly established that, while HSF2 had no transcriptional activity by itself, short β isoform of HSF2 exerts a negative role on HSF1β-dependent transactivation. To further assess the impact of HSF2β inhibition on HSF1 activity, we developed a mathematical modelling approach which revealed that the balance between each HSF isoform in the cell regulated the strength of the transcriptional response. Moreover, we found that cellular stress such as proteasome inhibition could regulate the splicing of Hsf2 mRNA. All together, our results suggest that relative amounts of each HSF1 and HSF2 isoforms quantitatively determine the cellular level of the proteotoxic stress response.
ISSN: 1932-6203

inserm-00814784
http://www.hal.inserm.fr/inserm-00814784
http://www.hal.inserm.fr/inserm-00814784/document
http://www.hal.inserm.fr/inserm-00814784/file/journal.pone.0056085.pdf
DOI : 10.1371/journal.pone.0056085

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Le Costa Rica est devenu une référence incontournable sur le plan de l’écotourisme et de la conservation de l’environnement depuis une quinzaine d’années. Le pays est « célèbre pour son approche éclairée de la préservation de l’environnement ». Environ 27 % de la superficie du pays est protégée et les parcs nationaux couvrent plus de 13 % du territoire costaricien. À travers l’analyse des processus de développement du tourisme, l’exemple du Costa Rica démontre l’importance des facteurs politiques et du contrôle des images dans la mise en tourisme d’un lieu.
Costa Rica has become an essential reference point in terms of ecotourism and conservation of the environment over the past fifteen years. The country became famous for its original policy to preserve the environment. Approximately 27% of the country's land is protected and national parks cover more than 13% of the Costa Rican territory. An analysis of the Costa Rican approaches to the development of tourism, provides an example of the importance of political factors and the control of “image” in the popular and public imagination.
Costa Rica

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.432
http://journals.openedition.org/etudescaribeennes/432

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Interleukin-33 (IL-33), a cytokine belonging to the IL-1 family, is crucially involved in inflammatory pathologies including liver injury and linked to various modes of cell death. However, a link between IL-33 and necroptosis or programmed necrosis in liver pathology remains elusive. We aimed to investigate the regulation of IL-33 during necroptosis-associated liver injury. The possible regulation of IL-33 during liver injury by receptor-interacting protein kinase 1 (RIPK1) and poly(ADP-ribose) polymerase 1 (PARP-1) was investigated in mice in vivo and in hepatic stellate cells in vitro. The liver immunohistopathology, flow cytometry, serum transaminase measurement, ELISA, and qPCR-based cytokine measurement were carried out. By using a chemical approach, we showed that pretreatment of mice with Necrostatin-1 (Nec-1) (inhibitor of RIPK1) and/or PJ34 (inhibitor of PARP-1) significantly protected mice against concanavalin A (ConA) liver injury (aspartate amino-transferase (AST)/alanine amino-transferase (ALT)) associated with down-regulated hepatocyte-specific IL-33 expression. In contrast, the expression level of most systemic cytokines (except for IL-6) or activation of liver immune cells was not altered by chemical inhibitors rather an increased infiltration of neutrophils in the liver. During polyinosine-polycytidylic acid (Poly(I:C))-induced acute hepatitis, liver injury and hepatocyte-specific IL-33 expression was also inhibited by PJ34 without any protective effect of PJ34 in CCl4-induced liver injury. Moreover, PJ34 down-regulated the protein expression of IL-33 in activated hepatic stellate cells by cocktail of cytokines or staurosporine in vitro. In conclusion, we evidenced that the Nec-1/PJ34 is a potent inhibitor of liver injury and Nec-1/PJ34 down-regulated hepatocyte-specific IL-33 expression in the liver in vivo or in hepatic stellate cells in vitro, suggesting IL-33 as a possible readout of necroptosis-involved liver pathologies. Necroptosis inhibitors can protect mice against liver injury induced by ConA or Poly(I:C). IL-33 expression in liver injury in vivo is inhibited by PJ34. IL-33 expression in hepatic stellate cells in vitro is inhibited by PJ34. Hepatocyte-specific IL-33 expression is down-regulated by Nec-1/PJ34 during hepatitis. IL-33 is a new marker of necroptosis-associated liver injuries.
ISSN: 0946-2716

hal-01134319
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134319
DOI : 10.1007/s00109-015-1270-6
PUBMED : 25747661

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : L’évolution récente du tourisme international au Venezuela est particulièrement intéressante : l’activité touristique qui reste très largement concentrée sur la côte caribéenne, et notamment l’île de Margarita, se diversifie au profit de nouveaux centres d’intérêt dans les régions intérieures. L’affichage de nouvelles volontés politiques sur la scène régionale et internationale, sous l’impulsion du président Chavez, a perturbé le marché touristique national qui était dominé pour les clientèles nord-américaines ; le Venezuela apparaît désormais aux yeux des États-Unis comme un pays à risque, instable. C’est dans ce contexte que s’inscrit le « Programa Andes Tropicales » [PAT] qui soutient le développement rural dans les parcs nationaux ; ce programme repose sur la participation des populations locales et leur implication dans les politiques de conservation des ressources naturelles et culturelles sources de revenus. Le soutien financier des microprojets familiaux et communautaires doit permettre de lutter contre la pauvreté.
The recent evolution of international tourism in Venezuela is particularly interesting. Even if tourism activity remains heavily concentrated on the Caribbean coast, in particular on the island of Margarita, it is nevertheless diversifying gradually to make way for new areas of interest in the country’s interior. The display of new political will on the regional and international scene, under the leadership of President Chavez, has disrupted the national tourism market which was dominated by North American customers. Venezuela now appears in the eyes of the United States as a country that is at risk,and therefore unstable. It is in this context that the “Programa Andes Tropicales" [PAT] was developed to promote rural development in national parks; This program is based on the participation and involvement of local populations in the development of appropriate policies for conserving natural resources and cultural sources of income. Financial support is also provided for family and community micro projects to help alleviate poverty in these regions.
Venezuela

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.445
http://journals.openedition.org/etudescaribeennes/445

Éditeur(s) : HAL CCSD American Mathematical Society
Résumé : International audience
We show that if n sets in a topological space are given so that all the sets are closed or all are open, and for each k ≤ n every k of the sets have a (k − 2)-connected union, then the n sets have a point in common. As a consequence, we obtain the following starshaped version of Helly's theorem: If every n + 1 or fewer members of a finite family of closed sets in Rn have a starshaped union, then all the members of the family have a point in common. The proof relies on a topological KKM-type intersection theorem.
ISSN: 0002-9939

hal-00699217
https://hal.archives-ouvertes.fr/hal-00699217

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Beggiatoaceae, giant sulphur-oxidizing bacteria, are well known to occur in cold and temperatewaters, as well as hydrothermal vents, where they form dense mats on the floor.However, they have never been described in tropical marine mangroves. Here, we describetwo new species of benthic Beggiatoaceae colonizing a marine mangrove adjacent to mangroveroots. We combined phylogenetic and lipid analysis with electron microscopy in orderto describe these organisms. Furthermore, oxygen and sulphide measurements in and exsitu were performed in a mesocosm to characterize their environment. Based on this, two new species, Candidatus Maribeggiatoa sp. and Candidatus Isobeggiatoa sp. inhabiting tropical marine mangroves in Guadeloupe were identified. The species identified as CandidatusMaribeggiatoa group suggests that this genus could harbour a third cluster with organisms ranging from 60 to 120 μm in diameter. This is also the first description of an Isobeggiatoa species outside of Arctic and temperate waters. The multiphasic approach also gives information about the environment and indications for the metabolism of these bacteria. Our study shows the widespread occurrence of members of Beggiatoaceae family and provides new insight in their potential role in shallow-water marine sulphide-rich environments such as mangroves.
ISSN: 1932-6203

hal-01205641
https://hal.univ-antilles.fr/hal-01205641
https://hal.univ-antilles.fr/hal-01205641/document
https://hal.univ-antilles.fr/hal-01205641/file/Beggiatoa%20Plosone2015.pdf
DOI : 10.1371/journal.pone.0117832

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The Hedgehog (Hh) family of secreted proteins act as morphogens to control embryonic patterning and development in a variety of organ systems. Post-translational covalent attachment of cholesterol and palmitate to Hh proteins are critical for multimerization and long range signaling potency. However, the biological impact of lipid modifications on Hh ligand distribution and signal reception in humans remains unclear. In the present study, we report a unique case of autosomal recessive syndromic 46,XY Disorder of Sex Development (DSD) with testicular dysgenesis and chondrodysplasia resulting from a homozygous G287V missense mutation in the hedgehog acyl-transferase (HHAT) gene. This mutation occurred in the conserved membrane bound O-acyltransferase (MBOAT) domain and experimentally disrupted the ability of HHAT to palmitoylate Hh proteins such as DHH and SHH. Consistent with the patient phenotype, HHAT was found to be expressed in the somatic cells of both XX and XY gonads at the time of sex determination, and Hhat loss of function in mice recapitulates most of the testicular, skeletal, neuronal and growth defects observed in humans. In the developing testis, HHAT is not required for Sertoli cell commitment but plays a role in proper testis cord formation and the differentiation of fetal Leydig cells. Altogether, these results shed new light on the mechanisms of action of Hh proteins. Furthermore, they provide the first clinical evidence of the essential role played by lipid modification of Hh proteins in human testicular organogenesis and embryonic development.
ISSN: 1553-7390

hal-01063929
https://hal.archives-ouvertes.fr/hal-01063929
DOI : 10.1371/journal.pgen.1004340
PUBMED : 24784881

Éditeur(s) : Société Française d'Ichtyologie
Résumé : Following the volcanic eruption of April 2007, nine specimens of Argyripnus (family Sternoptychidae, order Stomiiformes) were among the fish caught at the sea surface at Reunion Island (Indian Ocean). Seven species were described in this genus: A. ephippiatus Gilbert & Cramer, 1897 from Hawaii, A. iridescens McCulloch, 1926 from Australia. A. atlanticus Maul, 1952 from Madeira, A. brocki Struhsaker, 1973 from Hawaii, Argyripnus sp. Yamamoto in Okamura et al., 1982 from Japan, A. electronus Parin, 1992 from the South-east Pacific and A. pharos Harold & Lancaster, 2003 from the Indian Ocean and the central-west Pacific. The Argyripnus from Reunion Island is easily distinguished from the other species by the position of the anus just before the beginning of the anal fin (about half distance between the pelvic base and the beginning of the anal fin in the other species). The new Argyripnus from Reunion Island is described under the name A. hulleyi. Some indications are given on the bathymetric and geographical distributions of all the Argyripnus species. After a brief comparison of the characteristics of the seven species, an identification key is provided.
Cybium (0399-0974) (Société Française d'Ichtyologie), 2009-03 , Vol. 33 , N. 1 , P. 39-43

Droits : 2009 Société Française d'Ichtyologie
http://archimer.ifremer.fr/doc/2009/publication-6600.pdf
http://archimer.ifremer.fr/doc/00000/6600/

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : La sous-préfecture de Madingo-Kayes est arrosée par le bassin du Kouilou-Niari. Celui-ci constitue une niche écologique d’une grande diversité d’espèces halieutiques. Ces espèces sont recherchées par les populations locales pour leur rôle d’appoint dans la lutte contre l’insécurité alimentaire et leur contribution aux moyens d’existence et aux économies rurales. Le poisson, dont la consommation fournit près de 70 % de protéines animales à Madingo-Kayes, provient essentiellement de la pêche artisanale (avec l’utilisation d’outils rudimentaires tels que la nasse, le filet, la ligne, etc.). La recette mensuelle générée par cette activité oscille entre 250 000 et 500 000 FCF, soit environ un million de FCFA par année. Les principales espèces capturées sont les poissons-chat électriques (Malapterurus electricus), les poissons de la famille des Mormyridae, etc. Elles sont soit consommées sur place, soit écoulées à Pointe-Noire, ville où la demande est très forte.
The sub-prefecture of Madingo-Kayes is watered by the Kouilou-Niari basin. This constitutes an ecological niche of a wide variety of fish species sought by local people for their supporting role in the fight against food insecurity and their contribution to livelihoods and rural economies. Fish consumption in Madingo-Kayes provides nearly 70% of animal protein. The monthly recipe fish production in the district is between 250 000 and 500 000 FCFA. It is estimated an average of one million a year. The fishery is artisanal, with the use of simple tools such as the trap, net, line, etc. The main species of fish are captured electric catfish (Malapterurus electricus), fish of the family Mormyridae, etc. They are either consumed in the localities for food reasons or passed in Pointe-Noire, the city where the fish demand is very strong for mercantile reasons.
Madingo-Kayes
Congo RD

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.10391
http://journals.openedition.org/etudescaribeennes/10391