Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We investigated the deformation mechanisms and the seismic properties of 10 eclogite samples from different localities (Alps, Norway, Mali and eastern China) through the analysis of their microstructures and lattice-preferred orientations (LPO). These samples are representative of various types and intensity of deformation under eclogitic metamorphic conditions. Omphacite and garnet LPO were determined from electron backscatter diffraction (EBSD) technique. Garnet appears to be almost randomly oriented whereas omphacite develops strong LPO, characterized by the [001]-axes concentrated sub-parallel to the lineation, and the (010)-poles concentrated sub-perpendicular to the foliation. In order to analyze the deformation mechanisms that produced such omphacite LPO, we compare our observations to LPO simulated by viscoplastic self-consistent numerical models. A good fit to the measured LPO is obtained for models in which the dominant slip systems are 1/2h110i{11 ¯ 0}, [001] {110} and [001] (100). Dominant activation of these slip systems is in agreement with TEM studies of naturally deformed omphacite. Seismic properties of eclogite are calculated by combining the measured LPO and the single crystal elastic constants of omphacite and garnet. Although eclogite seismic anisotropies are very weak (less than 3% for both P-and S-wave), they are generally characterized by a maximum P-wave velocity sub-parallel to the lineation and by a minimum velocity approximately normal to foliation. The mean P-and S-wave velocities are high (respectively, 8.6 and 4.9 km/s). The S-wave anisotropy pattern displays complex relationships with the structural frame but the fast polarization plane generally tends to be parallel to the foliation. Calculated reflection coefficients show that an eclogite/crust interface is generally a good reflector (Rc > 0.1), whereas an eclogite body embedded in the upper mantle would be hardly detectable.
ISSN: 0040-1951

hal-01389715
http://hal.univ-reunion.fr/hal-01389715
http://hal.univ-reunion.fr/hal-01389715/document
http://hal.univ-reunion.fr/hal-01389715/file/Bascou_eclo-sismo_tphy2001_hal.pdf
DOI : 10.1016/S0040-1951(01)00156-1

Résumé : Dans le cadre de ma dernière année en Bachelor Océanographe mention prospection du domaine marin à INTECHMER, j'ai pu réaliser un stage d'une durée de quatre mois au sein d'un grand organisme océanographique français : à l'IFREMER. J'ai effectué ce stage au sein du Laboratoire Physique des Océans de l'IFREMER Brest sous la tutelle de Pascale LHERMINIER, chercheuse IFREMER. Durant ce stage, j'ai participé à la mission OVIDE (Observation de la Variabilité Interannuelle à Décennale en Atlantique Nord) dont ma maître de stage était le chef de mission. Mon travail pendant cette campagne consistait principalement en la mesure et en la calibration de la salinité de 2767 échantillons prélevés selon une section allant de Lisbonne jusqu'à la pointe sud du Groenland. Ce rapport présente tout d'abord le laboratoire au sein duquel j'ai effectué mon stage puis le projet OVIDE et son contexte scientifique. Ensuite, je présenterai le déroulement de la campagne OVIDE 2006, ainsi que le travail que j'ai réalisé à bord avec d'une part l'acquisition de données, et d'autre part l'analyse et le traitement préliminaire de ces données toujours dans la problématique « Comment obtenir de bonnes données et des mesures de qualité.». Enfin, j'expliquerai ce que m'apporté ce stage, comment je me suis intégré au sein de l'équipe scientifique de la campagne OVIDE et au sein du LPO.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2006/rapport-6502.pdf
http://archimer.ifremer.fr/doc/00000/6502/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Ethnopharmacological relevancePsidium acutangulum Mart. ex DC is a small tree used by the Wayana Amerindians from the Upper-Maroni in French Guiana for the treatment of malaria.Aim of the studyIn a previous study, we highlighted the in vitro antiplasmodial, antioxidant and anti-inflammatory potential of the traditional decoction of P. acutangulum aerial parts. Our goal was then to investigate on the origin of the biological activity of the traditional remedy, and eventually characterize active constituents.Materials and methodsLiquid-liquid extractions were performed on the decoction, and the antiplasmodial activity evaluated against chloroquine-resistant FcB1 ([3H]-hypoxanthine bioassay) and 7G8 (pLDH bioassay) P. falciparum strains, and on a chloroquine sensitive NF54 ([3H]-hypoxanthine bioassay) P. falciparum strain. The ethyl acetate fraction (D) was active and underwent bioguided fractionation. All the isolated compounds were tested on P. falciparum FcB1 strain. In vitro anti-inflammatory activity (IL-1β, IL-6, IL-8, TNFα) of the ethyl acetate fraction and of an anti-Plasmodium active compound, was concurrently assessed on LPS-stimulated human PBMC and NO secretion inhibition was measured on LPS stimulated RAW murine macrophages. Cytotoxicity of the fractions and pure compounds was measured on VERO cells, L6 mammalian cells, PBMCs, and RAW cells.ResultsFractionation of the ethyl acetate soluble fraction (IC50 ranging from 3.4 to <1 µg/mL depending on the parasite strain) led to the isolation of six pure compounds: catechin and five glycosylated quercetin derivatives. These compounds have never been isolated from this plant species. Two of these compounds (wayanin and guaijaverin) were found to be moderately active against P. falciparum FcB1 in vitro (IC50 5.5 and 6.9 µM respectively). We proposed the name wayanin during public meetings organized in June 2015 in the Upper-Maroni villages, in homage to the medicinal knowledge of the Wayana population. At 50 µg/mL, the ethyl acetate fraction (D) significantly inhibited IL-1β secretion (−46%) and NO production (−21%), as previously observed for the decoction. The effects of D and guiajaverin (4) on the secretion of other cytokines or NO production were not significant.ConclusionsThe confirmed antiplasmodial activity of the ethyl acetate soluble fraction of the decoction and of the isolated compounds support the previous results obtained on the P. acutangulum decoction. The antiplasmodial activity might be due to a mixture of moderately active non-toxic flavonoids. The anti-inflammatory activities were less marked for ethyl acetate fraction (D) than for the decoction.
ISSN: 0378-8741

hal-01313006
http://hal.upmc.fr/hal-01313006
http://hal.upmc.fr/hal-01313006/document
http://hal.upmc.fr/hal-01313006/file/Houel_Wayanin_and.pdf
DOI : 10.1016/j.jep.2016.04.053

Éditeur(s) : Elsevier
Résumé : Red drum larvae were fed from day 11 to day 24 post-hatch, with Artemia (LP group) or two isoproteinic-formulated diets with varying lipid levels, 15% (L15 group) and 30% (L30 group). The lipid fraction was composed of a mixture of cod liver oil and soy lecithin. Larvae fed Artemia exhibited the greatest length, L30 diet induced a larvae length significantly greater than that obtained with L15 diet (P < 0,05). No difference was observed in larvae survival among the three dietary groups. The increase in dietary lipid concentration led to an increase in amylase and trypsin secretion levels in 24-day-old larvae. The pancreatic secretion level measured in L30 group was close to that of LP group, suggesting that the maturation of the pancreatic functions was normally developed. Red drum larvae fed the diet containing the higher lipid content (L30) exhibited a more developed brush border membrane digestion in the intestine than larvae fed L15 diet (P < 0.05). Phospholipase A2 was stimulated by the increase of phospholipids in the diet, but no difference was observed for lipase between the two compound diet-fed groups; the LP group exhibited the lowest lipase activity. A plateau in lipase activity may have occurred in red drum larvae from 15% fat level in the diet, suggesting that the maximal lipase activity was reached with 12% neutral lipid in the diet. The positive effects observed with high dietary fat may be due to the phospholipid fraction of the lipid mixture used.
Aquaculture (0044-8486) (Elsevier), 2000-04 , Vol. 184 , N. 3-4 , P. 339-347

Droits : 2000 Elsevier
http://archimer.ifremer.fr/doc/2000/publication-503.pdf
DOI:10.1016/S0044-8486(99)00325-7
http://archimer.ifremer.fr/doc/00000/503/

Éditeur(s) : Université Montpellier 2 - Sciences et Techniques du Languedoc
Résumé : The work reported here was motivated by the economical importance of the pacific blue shrimp Litopenaeus stylirostris farming where high mortality rates are due to bacterial and viral diseases. It consists in the characterisation of two original peptides, the first members of a new multifunctional family of peptides from peneide shrimps, the stylicines. Those two peptides, named stylicines 1 and 2, are negatively charged (pI < 6.0), and characterised by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. Stylicines are synthesized by heamocytes where they are stored within small cytoplasmic granules. To understand the role of these peptides in the immune response of shrimps to a vibrio infection, their recombinant forms were produced in E. coli BL21 (DE3) plysS, purified and characterised. The two rstylicines display biological anti-proliferative and blood clotting activities. Only rstylicine 1 displays antimicrobial activities: antifungal against Fusarium oxysporum (MIC<2.5μM) and bacteriostatic against Gram (−) bacteria, Vibrio sp. (MIC<80μM). Moreover this peptide displays an LPS-binding activity (dissociation constant (Kd) of 9.6×10−8 M) and agglutinate Vibrio. penaeicida "in vitro". Finally, the presence of sequences coding for modified forms of stylicine 1 in some shrimp’s genome may be in relation with their lower ability to survive infections.
Les travaux présentés dans ce mémoire ont été motivés par l’importance économique de l’élevage de la crevette bleue du pacifique Litopenaeus stylirostris dont les fortes mortalités sont principalement dues au développement de maladies bactériennes et virales. Ils ont consisté en la caractérisation des deux premiers membres d’une famille originale de peptides multifonctionnels présents chez les crevettes pénéides, les stylicines. Ces peptides, nommés stylicines 1 et 2, sont des peptides anioniques (pI < 6.0), formés d’une région amino-terminale riche en résidus de type proline et d’une région carboxyterminale riche de treize résidus cystéines. Ces molécules sont synthétisées et stockées dans de petits granules présents dans le cytoplasme des hémocytes. Pour mieux appréhender leurs rôles dans la réponse immunitaire des crevettes à une infection par des Vibrio, leurs formes recombinantes ont été produites dans E. coli BL21 (DE3) plysS, purifiées et caractérisées. Les deux rstylicines présentent des activités antiproliférative et anticoagulante. Seule la rstylicine1 présente des activités antimicrobiennes : antifongique sur Fusarium oxysporum (CMI<2.5 μM), et antibactérienne (bactériostatique) sur Vibrio sp (CMI<80 μM). Ce peptide est également capable de se lier aux LPS des bactéries à Gram (-) (Kd= 9.6x10-8 M) et d’agglutiner V. penaeicida "in vitro". Enfin, l’existence de gènes codant des formes modifiées de la stylicine1, chez certaines crevettes, pourrait être en relation avec une diminution de la résistante des individus aux infections.

Droits : 2010 The Author, Univ Montpellier 2
http://archimer.ifremer.fr/doc/00024/13558/10612.pdf
http://archimer.ifremer.fr/doc/00024/13558/

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
This paper aims to decipher the thermal evolution of the Montagne Noire Axial Zone (MNAZ,southern French Massif Central) gneiss core and its metasedimentary cover through determination ofP–T paths and temperature gradients. Migmatitic gneiss from the core of the dome record a clock-wise evolution culminating at 725 25 °C and 0.8 0.1 GPa with partial melting, followed by adecompression path with only minor cooling to 690 25° C and 0.4 0.1 GPa. Field structuralanalyses as well as detailed petrological observations interpreted in a petrogenetic grid indicate thatthe cover sequence experienced LP-HT metamorphism at <0.4 GPa. Apparent thermal gradientswithin the cover were determined with garnet–biotite thermometry and Raman Spectroscopy on Car-bonaceous Matter. High-temperature apparent gradients (e.g. 530 °C km1 along one transect) areexplained by late brittle–ductile extensional shearing evidenced by phyllonites that post-date peakmetamorphism. In areas where normal faults are less abundant and closely spaced, gradients of 20to 50 °C km1 are calculated. These gradients can be accounted for by a combination of domeemplacement and ductile shearing (collapse of isotherms), without additional heat input. Finally, thethermal evolution of the MNAZ is typical for many gneiss domes worldwide as well as with otherLP-HT terranes in the Variscides.
ISSN: 0263-4929

Droits : http://creativecommons.org/licenses/by-nc-nd/
insu-01290388
https://hal-insu.archives-ouvertes.fr/insu-01290388
https://hal-insu.archives-ouvertes.fr/insu-01290388/document
https://hal-insu.archives-ouvertes.fr/insu-01290388/file/jmg12188.pdf
DOI : 10.1111/jmg.12188

Éditeur(s) : HAL CCSD AGU and the Geochemical Society
Résumé : International audience
Peridotite xenoliths brought up to the surface by the volcanism of the Kerguelen Islands represent a mantle that has been affected by a high degree of partial melting followed by intense melt percolation above the Kerguelen plume. These xenoliths are therefore particularly suitable to investigate effects of melt-rock interaction on crystallographic fabrics (lattice-preferred orientation (LPO)) of peridotite minerals and on the LPO-induced seismic properties of peridotites above a mantle plume. We have studied a suite of 16 ultramafic samples representative of different degrees of partial melting and magma-rock interaction among which the protogranular harzburgites are the least metasomatised xenoliths and dunites are the ultimate stage of metasomatism. Olivine LPO is characterized by high concentration of [010] axes perpendicular to the foliation and [100] axes close to the lineation or distributed in the foliation plane in harzburgites, whereas the high concentration of [100] axes is parallel to the lineation and [010] axes is perpendicular to the assumed foliation in dunites. Olivine LPO in harzburgites is interpreted as being due to a deformation regime in axial compression or transpression. The fabric strength of olivine decreases progressively from protogranular to poikilitic harzburgites and finally to dunites, for which it remains nevertheless significant (J index 3.8). Seismic properties calculated from LPO of minerals indicate that metasomatism at higher melt/rock ratio lowers the P wave velocities. The most significant difference between harzburgites and dunites corresponds to the distribution of S wave anisotropy. Harzburgites display the maximum of anisotropy within the foliation plane and the minimum of anisotropy perpendicular to the foliation plane, whereas the lowest anisotropy is parallel to the lineation for dunites. These modifications of seismic properties as a result of metasomatic processes may induce seismic heterogeneities in the mantle above the Kerguelen plume. In addition, assuming a lithospheric mantle primarily harzburgitic and structured with a horizontal foliation, the seismic properties calculated for the Kerguelen xenoliths reconcile the rather high anisotropy evidenced by the horizontally propagating surface waves with the apparent isotropy revealed by the absence of splitting of vertically propagating teleseismic SKS waves recorded by the GEOSCOPE Kerguelen station.
ISSN: 1525-2027

hal-00366222
https://hal.archives-ouvertes.fr/hal-00366222
https://hal.archives-ouvertes.fr/hal-00366222/document
https://hal.archives-ouvertes.fr/hal-00366222/file/bascou_Kerguelen_2007GC001879_hal.pdf
DOI : 10.1029/2007GC001879

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
IODP Site U1309 was drilled at Atlantis Massif, an oceanic core complex, at 30 degrees N on the Mid-Atlantic Ridge (MAR). We present the results of a bulk rock geochemical study (major and trace elements) carried out on 228 samples representative of the different lithologies sampled at this location. Over 96% of Hole U1309D is made up of gabbroic rocks. Diabases and basalts cross-cut the upper part of the section; they have depleted MORB compositions similar to basalts sampled at MAR 30 degrees N. Relics of mantle were recovered at shallow depth. Mantle peridotites show petrographic and geochemical evidence of extensive melt-rock interactions. Gabbroic rocks comprise: olivine-rich troctolites (>70% modal olivine) and troctolites having high Mg# (82-89), high Ni (up to 2300 ppm) and depleted trace element compositions (Yb 0.06-0.8 ppm); olivine gabbros and gabbros (including gabbronorites) with Mg# of 60-86 and low trace element contents (Yb 0.125-2.5 ppm); and oxide gabbros and leucocratic dykes with low Mg# (<50), low Ni (similar to 65 ppm) and high trace element contents (Yb up to 26 ppm). Troctolites and gabbros are amongst the most primitive and depleted oceanic gabbroic rocks. The main geochemical characteristics of Site U1309 gabbroic rocks are consistent with a formation as a cumulate sequence after a common parental MORB melt, although (lack of systematic) downhole variations indicate that the gabbroic series were built by multiple magma injections. In detail, textural and geochemical variations in olivine-rich troctolites and gabbronorites suggest chemical interaction (assimilation?) between the parental melt and the intruded lithosphere. Site U1309 gabbroic rocks do not represent the complementary magmatic product of 30 degrees N volcanics, although they sample the same mantle source. The bulk trace element composition of Site U1309 gabbroic rocks is similar to primitive MORB melt compositions; this implies that there was no large scale removal of melts from this gabbro section. The occurrence of such a large magmatic sequence implies that a high magmatic activity is associated with the formation of Atlantis Massif. Our results suggest that almost all melts feeding this magmatic system stays trapped into the intruded lithosphere.
ISSN: 0012-821X

hal-00413562
https://hal.archives-ouvertes.fr/hal-00413562
DOI : 10.1016/j.epsl.2008.12.034

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Garnet growth in high-pressure, mafic garnet granulites formed by dehydration melting of hornblende-gabbronorite protoliths in the Jijal complex (Kohistan palaeo-island arc complex, north Pakistan) was investigated through a microstructural EBSD-SEM and HRTEM study. Composite samples preserve a sharp transition in which the low-pressure precursor is replaced by garnet through a millimetre-sized reaction front. A magmatic foliation in the gabbronorite is defined by mafic-rich layering, with an associated magmatic lineation defined by the shape-preferred orientation (SPO) of mafic clusters composed of orthopyroxene (Opx), clinopyroxene (Cpx), amphibole (Amp) and oxides. The shape of the reaction front is convoluted and oblique to the magmatic layering. Opx, Amp and, to a lesser extent, Cpx show a strong lattice-preferred orientation (LPO) characterized by an alignment of [001] axes parallel to the magmatic lineation in the precursor hornblende-gabbronorite. Product garnet (Grt) also displays a strong LPO. Two of the four < 111 > axes are within the magmatic foliation plane and the density maximum is subparallel to the precursor magmatic lineation. The crystallographic relationship < 111 >(Grt)//[001](Opx,Cpx,Amp) deduced from the LPO was confirmed by TEM observations. The sharp and discontinuous modal and compositional variations observed at the reaction front attest to the kinetic inhibition of prograde solid-state reactions predicted by equilibrium-phase diagrams. The P-T field for the equilibration of Jijal garnet granulites shows that the reaction affinities are 5-10 kJ mol.(-1) for the Grt-in reaction and 0-5 kJ mol.(-1) for the Opx-out reaction. Petrographic and textural observations indicate that garnet first nucleated on amphibole at the rims of mafic clusters; this topotactic replacement resulted in a strong LPO of garnet. Once the amphibole was consumed in the reaction, the parallelism of [001] axes of the mafic-phase reactants favoured the growth of garnet crystals with similar orientations over a pyroxene substrate. These aggregates eventually sintered into single-crystal garnet. In the absence of deformation, the orientation of mafic precursor phases conditioned the nucleation site and the crystallographic orientation of garnet because of topotaxial transformation reactions and homoepitaxial growth of garnet during the formation of high-pressure, mafic garnet-granulite after low-pressure mafic protoliths.
ISSN: 0263-4929

hal-00411323
https://hal.archives-ouvertes.fr/hal-00411323
DOI : 10.1111/J.1525-1314.2008.00789.x

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Upon subduction, the oceanic crust transforms to blueschists and eclogites, with seismic properties that gradually become similar to those of the surrounding mantle. In order to evaluate the anisotropy of blueschists and glaucophane-bearing eclogites, the elastic constants of glaucophane single-crystal plates from the Sesia-Lanzo Zone (Aosta Valley, Western Alps) were measured using Brillouin spectroscopy at ambient conditions. The mean P- and S-wave velocities are 7.8 and 4.6 km s−1 respectively, and the anisotropy is high (38.1% (AVP) and 27.3% (AVS)). Glaucophane develops strong LPO, characterized by the [001]-axes concentrated sub-parallel to the lineation, and the {110} poles concentrated sub-perpendicular to the foliation in both blueschist and eclogite rocks. The measured LPO is in good agreement with viscoplastic selfconsistent numerical models. Seismic properties of glaucophane-bearing blueschist and eclogite are calculated by combining measured LPO and the single-crystal elastic moduli of glaucophane with the other main mineral constituents of the rock: mostly epidote for blueschist, and omphacite and garnet for eclogite. Blueschists present stronger anisotropies (AVP=16.1% and AVS=10.3%) than eclogites (AVP=2.9% and AVS=1.7%). The shear-wave splitting and resulting delay times for a 7-km thick layer of eclogite or blueschist are low for the eclogite (b0.03 s), but signi!cant for blueschist (0.16 s). Application to the subducted oceanic crust yields a decrease of velocity contrast with the surrounding mantle and of anisotropy at depth depending on the temperature of the slab. The details of the velocity variations in subducted oceanic crust are dif!cult to reconcile with the blueschist-eclogite transition as probed by exhumed metamorphic rocks and may require additional phases such as lawsonite or chemical variations such as oxidation state.
ISSN: 0040-1951

hal-00564518
https://hal.archives-ouvertes.fr/hal-00564518
DOI : 10.1016/j.tecto.2010.09.011

Éditeur(s) : HAL CCSD
Résumé : Macrophages (MΦ), well-known to play a key role in immune response, also respond to environmental toxic chemicals such as diesel exhaust particles (DEP), an air pollutant recently classified as carcinogenic to humans. MΦ are heterogeneous and plastic cells which activate according to their microenvironment into either an M1 subtype (so called classically activated or pro-inflammatory) under IFNγ and LPS stimulation or an M2 subtype (so called alternatively activated or anti-inflammatory) under IL-4 and/or IL-13 stimulation. However, potential effects of DEPs on M1/M2 MΦ polarization remain poorly documented. First, we characterized the expression marker of in vitro M-CSF-differentiated MΦ from human monocytes and activated into the M1 or M2 subtypes. Our main results show that M-CSF-generated MΦ considered as anti-inflammatory are actually able to switch to an M1 phenotype after IFNγ/LPS stimulation. Furthermore, the markers identified in this study were used to assess the impact of organic extracts of DEP (DEPe) on MΦ polarization and more generally on their physiology. DEPe alter some M1 and M2 markers expressed by polarized MΦ, without causing the overall inhibition of the M1 and M2 polarization process or the switch to a different phenotype. This phenotype alteration is associated with a decrease in the LPS-dependent inflammatory response in M1 MΦ and the chemotactic capacities in M2 MΦ. DEPe decrease the secretion of some cytokines and chemokines such as IL-6, IL-12p40 and CCL18 via AhR and/or Nrf2 activation. At the same time, we show that M1 and M2 MΦ in response to DEPe are able to secrete a sufficient level of a pro-fibrotic growth factor, the platelet derived growth factor B (PDGF-B) via AhR activation, leading to stimulation of lung fibroblast proliferation. Finally, these works show that DEPe have immunotoxic properties with regards to the physiology of human in vitro polarized MΦ. This immunotoxicity may then contribute to the deleterious effects of these urban environmental contaminants on human health.
Les macrophages (MΦ), des cellules clefs de la réponse immunitaire peuvent répondre à des contaminants environnementaux comme les particules diesel (DEP), des polluants atmosphériques récemment classés cancérigènes pour l'Homme. Les MΦ sont des cellules hétérogènes et plastiques qui s'activent en fonction de leur microenvironnement soit en MΦ M1 (dits classiquement activés ou pro-inflammatoires) sous l'effet de l'INFγ et du LPS soit en MΦ M2 (dits alternativement activés ou réparateurs) sous l'effet de l'IL-4 et/ou de l'IL-13. Les effets des DEP sur la polarisation M1/M2 des MΦ restent peu documentés. Nous avons dans un premier temps caractérisé l'expression des marqueurs des MΦ différenciés in vitro en présence de M-CSF à partir de monocytes humains et polarisés en sous-type M1 ou M2. Nos principaux résultats montrent que les MΦ différenciés au M-CSF considérés comme des MΦ anti-inflammatoires, sont en réalité capables de s'activer vers un phénotype M1 après une stimulation au LPS/IFNγ. De plus, les marqueurs mis en évidence au cours de ce travail ont permis d'évaluer l'impact d'extraits organiques de DEP (DEPe) sur la polarisation des MΦ et plus généralement sur leur physiologie. Les DEPe altèrent l'expression de certains marqueurs M1 et M2 des MΦ, sans toutefois provoquer d'inhibition globale des processus de polarisation M1 et M2 ou de transition d'un phénotype vers un autre. Cette altération du phénotype est associée à une diminution de la réponse inflammatoire LPS-dépendante dans les MΦ M1 et des capacités chimiotactiques des MΦ M2. Les DEPe diminuent la sécrétion de certaines cytokines et chimiokines comme l'IL-6, l'IL-12p40 et le CCL18 via l'activation d'AhR et/ou de Nrf2. Parallèlement, nous montrons que les MΦ M1 et M2 exposés aux DEPe sécrètent le platelet deried growth factor B (PDGF-B), un facteur de croissance profibrosant, via l'activation d'AhR en quantité suffisante pour stimuler la prolifération de fibroblastes pulmonaires. Au total, ces travaux démontrent que les DEP possèdent des propriétés immunotoxiques vis-à-vis de la physiologie des macrophages humains polarisés in vitro. Cette immunotoxicité pourrait participer aux effets délétères de ces contaminants environnementaux urbains sur la santé humaine.
https://tel.archives-ouvertes.fr/tel-01188679

NNT : 2015REN1S011
tel-01188679
https://tel.archives-ouvertes.fr/tel-01188679
https://tel.archives-ouvertes.fr/tel-01188679/document
https://tel.archives-ouvertes.fr/tel-01188679/file/JAGUIN_Marie.pdf

Éditeur(s) : HAL CCSD Geological Society of London
Résumé : The Zagros Simply Folded Belt (ZSFB) is an active fold-and-thrust belt resulting from the still continuing continental collision between the Arabian plate and the Iranian plate, which probably started in the Oligocene. The present-day shortening (N25°) is well documented by focal mechanisms of earthquakes and global positioning system (GPS) surveys. We propose in this study a comparison of published palaeostress markers, including magnetic fabric, brittle deformation and calcite twinning data. In addition, we describe the magnetic fabric from Palaeocene carbonates (10 sites) and Mio-Pliocene clastic deposits (15 sites). The magnetic fabrics are intermediate, with magnetic foliation parallel to the bedding, and a magnetic lineation mostly at right angles to the shortening direction. This suggests that the magnetic fabric retains the record of an early layer-parallel shortening (LPS) that occurred prior to folding. The record of LPS allows the identification of originally oblique folds such as the Mand Fold, which have developed in front of the Kazerun Fault. The shape parameter of the magnetic fabric indicates a weak strain compatible with the development of detachment folds in the ZSFB. The palaeostress datasets, covering the Palaeocene to Pleistocene time interval, support several folding episodes accompanied by a counter-clockwise rotation of the stress field direction. The Palaeocene carbonates in the ZSFB record a N47 LPS during early to middle Miocene detachment folding in the High Zagros Belt (HZB). The Mio-Pliocene clastic deposits recorded a N38 LPS prior to and during detachment folding within the ZSFB at the end of the Miocene-Pliocene. Similarly, fault slip and calcite twin data from the ZSFB also support a counter-clockwise rotation from NE to N20 between the pre-folding stage and the late rejuvenation of folds. This counter-clockwise trend of palaeostress data agrees with fault slip data from the HZB. During the late stage of folding in the ZSFB, the Plio-Quaternary palaeostress trends are consistently parallel to the present-day shortening direction.
Tectonic and stratigraphic evolution of Zagros and Makran during the Mesozoic-Cenozoic

hal-00545036
https://hal.archives-ouvertes.fr/hal-00545036
DOI : 10.1144/SP330.6

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Background & Aims: The severity of liver diseases is exacerbated by the death of hepatocytes, which can be induced by the sensing of pathogen associated molecular patterns (PAMPs) derived from the gut microbiota. The molecular mechanisms regulating these cell death pathways are poorly documented. In this study, we investigated the role of the receptor interacting protein kinase 1 (RIPK1), a protein known to regulate cell fate decisions, in the death of hepatocytes using two in vivo models of PAMP-induced hepatitis. Methods: Hepatitis was induced in mice by independent injections of two different bacterial PAMPs: lipopolysaccharide (LPS) and unmethylated CpG oligodeoxynucleotide (CpG-DNA) motifs. The role of RIPK1 was evaluated by using mice specifically lacking RIPK1 in liver parenchymal cells (Ripk1(LPC-KO)). Administration of liposome-encapsulated clodronate served to investigate the role of Kupffer cells in the establishment of the disease. Etanercept, a tumor necrosis factor (TNF)-decoy receptor, was used to study the contribution of TNF-alpha during LPS-mediated liver injury. Results: Whereas RIPK1 deficiency in liver parenchymal cells did not trigger basal hepatolysis, it greatly sensitized hepatocytes to apoptosis and liver damage following a single injection of LPS or CpG-DNA. Importantly, hepatocyte death was prevented by previous macrophage depletion or by TNF inhibition. Conclusions: Our data highlight the pivotal function of RIPK1 in maintaining liver homeostasis in conditions of macrophage-induced TNF burst in response to PAMPs sensing. Lay summary: Excessive death of hepatocytes is a characteristic of liver injury. A new programmed cell death pathway has been described involving upstream death ligands such as TNF and downstream kinases such as RIPK1. Here, we show that in the presence of LPS liver induced hepatic injury was due to secretion of TNF by liver macrophages, and that RIPK1 acts as a powerful protector of hepatocyte death. This newly identified pathway in the liver may be helpful in the management of patients to predict their risk of developing acute liver failure. (C) 2017 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
ISSN: 0168-8278

hal-01532060
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01532060
DOI : 10.1016/j.jhep.2017.01.005
PUBMED : 28088582

Éditeur(s) : HAL CCSD
Résumé : Based on fieldwork and modern analytical technics, this work presents an overview of the Mid-Cretaceous hyper-extended rift system exposed in the northern part of the Pyrenean mountain range. Its inversion during the Pyrenean orogeny allows for precious observations of the deep-seated processes occurring at the foot of distal margins. Some peculiar aspects characterizing the pre-Alpine hyper-extended domain are examined throughout the 400km long suture that is fossilized in the North Pyrenean Zone (NPZ). The High Temperature/Low Pressure (HT/LP) thermal imprint of the extensional event is studied thanks to a dataset of more than hundred peak temperature measurement by Raman spectroscopy of the carbonaceous material (RSCM). The pre- and syn-rift metasediments are characterized by an intense ductile deformation. Field and EBSD investigations of the mechanisms of deformation reveal ductile and syn-metamorphic processes. The emplacement conditions of the peridotite into crustal levels and localized exhumation is examined in the key-localities of Urdach, Saraillé-Tos de la Coustette, Moncaup, Lherz, Caussou-Bestiac and Salvezines, The results indicate an early boudinage of the crust during the rifting process, resulting in the direct superposition of pre-rift sediments on top of mantle peridotites. Field, textural and isotopic characterization of the various ophicalcite and breccias found in the vicinity of the peridotite outcrops allow to decipher their exhumation history. New Ar-Ar geochronological results allow to precise the temporal evolution of the NPZ.
Cette thèse présente une analyse structurale et thermique des marges passives crétacées inversées dans la Zone Nord Pyrénéenne (ZNP). L'application du paléothermomètre RSCM (spectrométrie Raman de la matière carbonée) sur plus de cent échantillons répartis à travers la ZNP permet de préciser l'évolution thermique de la déchirure continentale. Un métamorphisme de haute température (450 à >600°C) se développe particulièrement au droit de zones ou la croûte continentale est extrêmement amincie voire éliminée. Les sédiments pré- et syn-rift sont marqués par une intense déformation ductile. L'analyse à l'EBSD des fabriques minérales indique une déformation de Haute-température contemporaine du métamorphisme HT-BP. Les conditions de mise en place crustale des péridotites et leur exhumation localisée sont examinées dans les localités d'Urdach, Saraillé-Tos de la Coustette, Moncaup, Lherz, Caussou-Bestiac et Salvezines. Les résultats indiquent un boudinage de la croûte continentale au cours du processus de rifting. Des décollements de couverture, principalement localisés dans les évaporites triasiques conduisent à la superposition directe des sédiments pré-rift sur le manteau exhumé. La notion nouvelle de soutirage crustal est introduite pour désigner ce processus dans lequel l'écoulement gravitaire ne joue pas un rôle principal. La caractérisation sédimentologique, pétrographique et isotopique (O et C) des différentes formations de brèches et ophicalcites observées à proximité des corps de péridotites permet de reconstituer les étapes de l'exhumation du manteau en pied de marge. Enfin de nouvelles datations permettent de mieux contraindre le calendrier des évènements successifs.
https://tel.archives-ouvertes.fr/tel-00787952

tel-00787952
https://tel.archives-ouvertes.fr/tel-00787952
https://tel.archives-ouvertes.fr/tel-00787952/document
https://tel.archives-ouvertes.fr/tel-00787952/file/TheI_se-Clerc-2012.pdf

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
The anisotropic single crystal seismic properties are reviewed in the light of recent experimental and theoretical determinations. Although considerable progress has been made on the determination of single crystal properties, data are still lacking, particularly for the temperature derivatives of transition zone and lower mantle phases. The common types of LPO of olivine, opx and cpx are presented together with their associated seismic properties. It is emphasized that simple seismic symmetry pattern of upper mantle rocks are a direct result of the interaction of olivine, opx and cpx. Using the standard structural frame (X lineation, Z pole to foliation) for typical LPOs of olivine, opx and cpx have the maximum Vp parallel to X, in the XZ plane and parallel to Y respectively. Destructive interference occurs between these minerals and hence P-wave anisotropy should be sensitive to the aggregate composition. For shear wave splitting (dVs) typical olivine and opx LPOs result in similar patterns with the maximum dVs in the YZ plane and the fast split shear wave (Vs1) polarized parallel to the foliation. A typical cpx LPO on the other hand produces destructive interference as the max dVs is close to X. By comparison with experiments and numerical simulations, it is estimated that upper mantle samples have an olivine LPO strength which recorded shear strain gamma of between 0.25 and 2.0. Pyrolite and piclogite models are compared with global transverse isotropic models. The slowly reducing P-wave anisotropy in the first 200 km can be explained by a model with constant composition and LPO strength. The sharp decrease in the observed anisotropy in the global models cannot be explained by the transformation of opx to cpx at 300 km, it is proposed that this decrease is due to a reduction in LPO strength from 200 to 350 km at the base of the lithosphere.
Earth's Deep Interior: Mineral Physics and Tomography From the Atomic Scale to the Global Scale

ISBN : 9780875909752
hal-01391524
http://hal.univ-reunion.fr/hal-01391524
http://hal.univ-reunion.fr/hal-01391524/document
http://hal.univ-reunion.fr/hal-01391524/file/Mainprice_AGUmonog_DM_GB_WBI_2000.pdf

Éditeur(s) : HAL CCSD Federation of American Society of Experimental Biology
Résumé : International audience
The innate immune system is able to detect bacterial LPS through the pattern recognition receptor CD14, which delivers LPS to various TLR signaling complexes that subsequently induce intracellular proinflammatory signaling cascades. In a previous study, we showed the overproduction of the soluble form of CD14 (sCD14) by macrophages from patients with cystic fibrosis (CF). CF is an autosomal recessive disorder that is caused by mutations in the gene that encodes the CFTR protein and is characterized by persistent inflammation. Macrophages play a significant role in the initial stages of this disease due to their inability to act as suppressor cells, leading to chronic inflammation in CF. In this work, we investigated the origin of sCD14 by human macrophages and studied the effect of sCD14 on the production of inflammatory cytokine/chemokine. Our data indicate that sCD14 stimulate proinflammatory cytokine/chemokine production in a manner that is independent of LPS but dependent on the TLR-4/CD14 membrane complex, NF-κB, and the inflammasome. Therefore, sCD14, overproduced by CF macrophages, originates primarily from the endocytosis/exocytosis process and should be considered to be a danger-associated molecular pattern. This elucidation of the origin and inflammation-induced mechanisms associated with sCD14 contributes to our understanding of maintained tissue inflammation.
ISSN: 0892-6638

hal-01526434
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01526434
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01526434/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01526434/file/Soluble%20CD14%20acts%20as%20a%20DAMP%20in%20human-accepted.pdf
PUBMED : 28122919

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
We present a new interpretation of anisotropy of magnetic susceptibility (AMS) fabrics in basaltic lava flows based on the detailed study of magnetic mineralogy and silicate crystallographic fabric of a Quaternary lava flow from the French Massif Central (La Palisse). We consider the model of AMS fabric imbrication between magnetic foliation and flow surface, as initially proposed for dykes. At the two sampling sites, the concordance between the flow direction deduced from the AMS foliation and that deduced from field observations indicates that the imbrication model could apply to the lava flows. However, the flow senses inferred fromAMSare systematically opposed between the two sampling sites suggesting permutations between K1 and K3 AMS axes, a configuration referred to as inverse fabric. Electron backscatter diffraction (EBSD) measurements show strong lattice-preferred orientations (LPO) for plagioclase, especially the (010) plagioclase plane, which tends to be parallel to the flow. Clinopyroxene LPO remains less marked than plagioclase LPO, whereas titanomagnetite does not display a significant LPO. Comparison between magnetic and crystallographic fabrics suggests that the AMS fabric of the lava flow results from the distribution of titanomagnetite grains, which is in turn controlled by the fabric of the silicate framework. Magnetic hysteresis parameters and anisotropy of remanent magnetization (ARM) measurements exclude a significant contribution from single-domain grains, often called upon to explain inverse magnetic fabrics. The origin of the observed inverse magnetic fabric may relate to the dip of the palaeosurface, which is the only remarkable difference between the two sampling sites. AMS appears as a good tool to determine the direction of basaltic lava flows and coupling with local crystallographic fabric data provides a valuable control of relationships between magnetic fabrics and flow and thus contributes to better constrain the AMS signature of lava flows.
ISSN: 0956-540X

hal-00903561
https://hal.archives-ouvertes.fr/hal-00903561
https://hal.archives-ouvertes.fr/hal-00903561/document
https://hal.archives-ouvertes.fr/hal-00903561/file/TB-GJI-193-2-Orig.pdf
DOI : 10.1093/gji/ggs115

Éditeur(s) : HAL CCSD Royal Society of Chemistry
Résumé : International audience
A versatile sol-gel route has been developed for the preparation of hierarchical porous silica membrane material with highly dispersed platinum nanoparticles (Pt-NPs). The outstanding feature of the "one pot" synthesis developed in this work lies in the successful preparation of a stable complex suspension made of silica sol, multi-scale porogens and Pt nanoparticles. The multi-scale porogens were based on non-ionic triblock copolymers and aqueous latex suspension to create mesopores and macropores, respectively, with finely tuned pore size and organization. For further functionalization of the membranes, Pt nanoparticles of about 4 nm in diameter were prepared by irradiating with microwaves (MW) the Pt precursors added in the starting suspension. This original path is suggested to enable a pre-organization of Pt nanoparticles in the ordered mesoporous structure. The suspensions were then deposited as thin films on either dense or macroporous supports, and further thermally treated at 450°C to remove the porogen units. Hierarchical porous layers composed of micropores (<2 nm), ordered mesopores (∼4 nm) and macropores (∼70 nm) with or without Pt were prepared and characterized. The resulting membranes are foreseen to exhibit great potential as multifunctional membranes for gas separation coupled with catalytic reaction. © The Royal Society of Chemistry 2008.
ISSN: 0959-9428

hal-01680586
https://hal.univ-antilles.fr/hal-01680586
DOI : 10.1039/b807029f

Éditeur(s) : HAL CCSD Wiley Open Access
Résumé : 11 pages
International audience
The loss of regulating agents such as parasites is among the most important changes in biotic interactions experienced by populations established in newly colonized areas. Under a relaxed parasite pressure, individuals investing less in costly immune mechanisms might experience a selective advantage and become successful colonizers as they re-allocate resources to other fitness-related traits. Accordingly, a refinement of the evolution of increased competitive ability (EICA) hypothesis proposed that immunity of invasive populations has evolved toward a reduced investment in innate immunity, the most costly component of immunity, and an increased humoral immunity that is less costly. Biogeographical approaches comparing populations between native and expansion ranges are particularly relevant in exploring this issue, but remain very scarce. We conducted a biogeographical comparison between populations of Spectacled Thrush (Turdus nudigenis) from the native area (South America) and from the expansion range (Caribbean islands). First, we compared haemosporidian prevalence and circulating haptoglobin (an acute-phase protein produced during inflammation). Second, we challenged captive birds from both ranges with Escherichia coli lipopolysaccharides (LPS) and measured postchallenge haptoglobin production and body mass change. Birds from the expansion range showed lower haemosporidian prevalence and lower levels of haptoglobin than birds from the native range. In addition, the inflammation elicited by LPS injection and its associated cost in terms of body mass loss were lower in birds from the expansion range than in birds from the native range. In accordance with the enemy release hypothesis, our results suggest that range expansion is associated with a reduced infection risk. Our study also supports the hypothesis that individuals from newly established populations have evolved mechanisms to dampen the inflammatory response and are in accordance with one prediction of the refined EICA hypothesis, proposed to understand biological invasions.
ISSN: 2045-7758

hal-01403291
https://hal.archives-ouvertes.fr/hal-01403291
DOI : 10.1002/ece3.2486

Éditeur(s) : HAL CCSD National Institute of Environmental Health Sciences
Résumé : International audience
Background: Glycol ethers (GE) are widely used organic solvents. Despite the potential neurotoxicity of several families of organic solvents, little is known about the impact of GE on the neurodevelopment of infants and children. Objectives: We investigated the relation between urinary concentrations of GE metabolites in pregnant women and neurocognitive abilities in their 6-year-old children in the PELAGIE mother-child cohort. Methods: Five GE metabolites were measured in first morning void urine samples of 204 French pregnant women in early pregnancy (<19 weeks of gestation). Psychologists assessed the neurocognitive abilities of their 6-year-old children with the Wechsler Intelligence Scale for Children IV (WISC) and the Developmental Neuropsychological Assessment (NEPSY). We analyzed the results with linear (WISC) and Poisson regression models (NEPSY), adjusted for potential confounders, including child's stimulation at home. Results: GE metabolites were detected in 90-100% of maternal urine samples. The WISC Verbal Comprehension score was significantly lower for children with the highest tertile of urinary phenoxyacetic acid (PhAA) [beta (third vs. first tertile)=-6.53; 95% CI: -11.44, -1.62]. Similarly, the NEPSY Design Copying subtest score was lower in those with the highest tertile of urinary ethoxyacetic acid (EAA) [beta (third vs. first tertile)=-0.11; 95% CI: -0.21, 0.00]. The other GE metabolites we studied were not significantly associated with WISC or NEPSY scores. Conclusions: Prenatal urine concentrations of two GE metabolites were associated with lower WISC Verbal Comprehension Index scores and NEPSY Design Copying subscale scores, respectively, at age 6 years. PhAA is the primary metabolite of 2-phenoxyethanol (EGPhE), which is commonly found in cosmetics, and precursors of EAA are frequently used in cleaning agents. Additional research is needed to confirm our findings and further explore potential effects of prenatal GE exposures on neurocognitive performance in children.
ISSN: 0091-6765

hal-01519256
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01519256
DOI : 10.1289/EHP39
PUBMED : 27740510