Éditeur(s) : HAL CCSD
Résumé : International audience
The development of diabetic vascular complications is initiated, at least in part, by mitochondrial reactive oxygen species (ROS) production in endothelial cells. Hyperglycemia induces superoxide production in the mitochondria and initiates changes in the mitochondrial membrane potential that leads to mitochondrial dysfunction. Hydrogen sulfide (H2S) supplementation has been shown to reduce the mitochondrial oxidant production and shows efficacy against diabetic vascular damage in vivo. However, the half-life of H2S is very short and it is not specific for the mitochondria. We have therefore evaluated two novel mitochondria-targeted anethole dithiolethione and hydroxythiobenzamide H2S donors (AP39 and AP123 respectively) at preventing hyperglycemia-induced oxidative stress and metabolic changes in microvascular endothelial cells in vitro. Hyperglycemia (HG) induced significant increase in the activity of the citric acid cycle and led to elevated mitochondrial membrane potential. Mitochondrial oxidant production was increased and the mitochondrial electron transport decreased in hyperglycemic cells. AP39 and AP123 (30–300 nM) decreased HG-induced hyperpolarisation of the mitochondrial membrane and inhibited the mitochondrial oxidant production. Both H2S donors (30–300 nM) increased the electron transport at respiratory complex III and improved the cellular metabolism. Targeting H2S to mitochondria retained the cytoprotective effect of H2S against glucose-induced damage in endothelial cells suggesting that the molecular target of H2S action is within the mitochondria. Mitochondrial targeting of H2S also induced >1000-fold increase in the potency of H2S against hyperglycemia-induced injury. The high potency and long-lasting effect elicited by these H2S donors strongly suggests that these compounds could be useful against diabetic vascular complications. © 2016
Pharmacological Research

hal-01373947
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01373947
DOI : 10.1016/j.phrs.2016.08.019
PUBMED : 27565382

Éditeur(s) : Wiley
Résumé : This paper investigates some economic consequences of creating a marine reserve on both fishing and ecotourism, when the range of controllability of fishing effort is limited and the impact of the reserve on ecosystem is considered. The issue is illustrated by the example of creating a no-take zone in part of a region where fishing is managed through a limited entry license system, and which is inhabited by two interacting stocks: a stock of prey (fish) and a stock of predators (seals). While the former is targeted by commercial fishing, the latter is not subject to harvest but is a potential basis for a commercial non-extractive activity (seal watching). Analysis is conducted with the help of a bioeconomic model combining the features of marine reserve modeling and of multispecies modeling. Following a description of the model, results of several simulation runs are presented. These show that creating a marine reserve has more complex economic implications than predicted in studies focused exclusively on one stock and/or commercial fisheries. More specifically, the model shows that the dynamics of the two interacting stocks reduces the benefits of the no-take zone for the fishing industry, while it makes the creation of this zone provide an opportunity for the development of ecotourism. Due to this dynamics, the model suggests that the optimal size of the reserve is larger when ecotourism is taken into account along with fishing activities.
Natural Resource Modeling (1939-7445) (Wiley), 2002-12 , Vol. 15 , N. 4 , P. 387–411

Droits : 2002 Rocky Mountain Mathematics Consortium
http://archimer.ifremer.fr/doc/00244/35506/34010.pdf
DOI:10.1111/j.1939-7445.2002.tb00095.x
http://archimer.ifremer.fr/doc/00244/35506/

Éditeur(s) : EDP Sciences
Résumé : From April 2003 to April 2004 monthly sea surveys were conducted around 2 fish aggregating devices (FADs) moored at 2000 and 2500 m depth in Martinique (Lesser Antilles). The use of a dual frequency splitbeam echosounder combined with an underwater camera and fishing methods allowed assessment of average space and time distribution of pelagic fish aggregated beneath the FADs, as well as identification of their overall size and species composition. At daytime, 4 fish aggregations were identified at each FAD, representing 4 distinct types: i) an aggregation of small juvenile tuna (mean fork length, FL: 30 cm) observed very close to the surface in 25% of daytime periods; ii) a small surface aggregation dominated by carangids, Caranx crysos, present in 65% of daytime periods; iii) a large sub-surface aggregation observed during all daytime periods: this aggregation appeared to be primarily comprised of 58 cm FL blackfin tuna (Thunnus atlanticus), mixed with yellowfin (Thunnus albacares) and skipjack (Katsuwonus pelamis) tunas of the same size; and iv) sub-surface scattered large predators (mainly blue marlin, Makaira nigricans) present in 10% of daytime periods. A smaller sub-surface aggregation comprised of medium tuna mixed with "extranatants" (fishes which remain within 10 to 50 m of a FAD) was observed in 75% of night-time periods, whereas unidentified scattered fishes were detected from 70 to 400 m depth. The low daytime vulnerability of medium sub-surface tunas to applied line techniques leads us to assume that their feeding motivation was low during daytime. These tunas could preferentially feed on mesopelagic organisms during night-time and transition periods around Martinican moored FADs. Local fishermen mainly targeted the large scattered predators using very small tunas as living bait. The sub-surface tuna aggregation hence appeared to be currently unexploited by local fisheries, though it represented the large majority of the pelagic biomass around the moored FADs.
Des campagnes scientifiques mensuelles ont été menées autour de 2 dispositifs de concentration de poissons ancrés à 2000-2500 m de profondeur, en Martinique d'avril 2003 à avril 2004. L'utilisation combinée d'un échosondeur monofaisceau multifréquence, d'une caméra vidéo sous-marine et de techniques de pêche conventionnelles a permis de déterminer la distribution spatio-temporelle moyenne des poissons agrégés autour des DCP ancrés ainsi que la composition générale des agrégations. Les techniques de vidéo sous-marine ont joué un rôle majeur dans l'identification des espèces et l'observation de leur comportement. De jour; quatre types d'agrégations ont été définis : i) une agrégation de thonidés juvéniles (longueur à la fourche, LF, moyenne : 30 cm) observée très près de la surface durant 25 % des périodes diurnes échantillonnées ii) une petite agrégation composée essentiellement de Caranx crysos présente en surface lors de 65 % des phases diurnes iii) une grande agrégation de thons observée en sub-surface lors de toutes les phases diurnes; cette agrégation était constituée essentiellement de thons noirs (Thunnus atlanticus) de 58 cm LF, associés de façon saisonnière à des albacores (Thunnus albacares) et des listaos (Katsuwonus pelamis) de taille similaire iv) des grands prédateurs assez dispersés (essentiellement le marlin bleu, Makaira nigricans), observés lors de 10 % des phases diurnes. Une agrégation de sub-surface, aux dimensions plus réduites et composée de thons et "d'extranatants" (poissons restant à distance de 10 à 50 m du DCP) a été observée lors de 75 % des périodes nocturnes; ainsi que des organismes non-identifiés dispersés entre 70 et 400 m de profondeur. La faible vulnérabilité diurne des thons de sub-surface aux techniques de ligne utilisées indique qu'ils ne se nourrissent pas activement durant la journée. Ces poissons pourraient se nourrir préférentiellement d'organismes mésopélagiques durant les phases de transition et nocturnes autour des DCP ancrés. La pêcherie artisanale martiniquaise n'exploite qu'une faible part des ressources agrégées autour des DCP ancrés. Les principales espèces-cibles sont les grands prédateurs capturés de jour en utilisant des thonidés juvéniles comme appât vivant. L'agrégation de thons de sub-surface représente la grande majorité de la biomasse autour des DCP mais elle est quasiment inexploitée par les pêcheries locales.
Aquatic Living Resources (0990-7440) (EDP Sciences), 2007-10 , Vol. 20 , N. 4 , P. 357-366

Droits : EDP Sciences, IFREMER, IRD 2007
http://archimer.ifremer.fr/doc/2007/publication-3634.pdf
DOI:10.1051/alr:2008004
http://archimer.ifremer.fr/doc/00000/3634/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Meconium is the first fecal excretion of newborns. This complex accumulative matrix allows assessing the exposure of the fetus to xenobiotics during the last 6 months of pregnancy. To determine the eventual effect of fetal exposure to micropollutants in this matrix, robust and sensitive analytical methods must be developed. This article describes the method development of liquid chromatography methods coupled to triple quadrupole mass spectrometry for relevant pollutants. The 28 selected target compounds had different physico-chemical properties from very polar (glyphosate) to non-polar molecules (pyrethroids). Tests were performed with three different types of columns: reversed phase, ion exchange and HILIC. As a unique method could not be determined for the simultaneous analysis of all compounds, three columns were selected and suitable chromatographic methods were optimized. Similar results were noticed for the separation of the target compounds dissolved in either meconium extract or solvent for reversed phase and ion exchange columns. However, for HILIC, the matrix had a significant influence on the peak shape and robustness of the method. Finally, the analytical methods were applied to “real” meconium samples.
ISSN: 0039-9140

hal-01141722
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01141722
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01141722/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01141722/file/Development%20of%20liquid%20chromatography%20%281%29.pdf
PUBMED : 25863396

Éditeur(s) : Oxford university press
Résumé : The response of fish assemblages to changes in protection status is a major issue for both biodiversity conservation and fishery management. In New Caledonia, the Abore reef marine reserve harbours more than 500 fish species, and has been subjected to changes in protection status since 1988. The present study investigates the impact of these changes on a wide subset of species (213), based on underwater visual counts collected before the opening and after the closure to fishing of this marine protected area (MPA). We analysed the spatial and temporal variability in fish assemblage attributable to protection status, explicitly considering habitat. To understand the successive responses of fish assemblage to fishing and protection, the assessment models included four criteria de. ning species groups that partition the fish assemblage: trophic regime, adult size, mobility, and interest for fishing. We could therefore identify the negative impact of opening the MPA to fishing on piscivores and highly mobile species. Surprisingly, target species were not affected more than non-target species. Model results were used to identify species groups that respond to fishing and protection. These results utilize fisheries-related criteria to provide new insight into the response of fish assemblages to protection from the perspective of MPA monitoring.
ICES Journal of Marine Science (1054-3139) (Oxford university press), 2009 , Vol. 66 , N. 1 , P. 170-179

Droits : 2009 ICES/CIEM. Oxford Journals
http://archimer.ifremer.fr/doc/2009/publication-6130.pdf
DOI:10.1093/icesjms/fsn204
http://archimer.ifremer.fr/doc/00000/6130/

Résumé : All the biological elements obtained in the two first phases of the study and the basical economical data are synthetized in a bio-economical model. From a typology of French fleets fishing in Celtic Sea, the carried out "métiers" (target-species + areas) are pointed out, which allows to define spatio-temporal "boxes". In each compartment, at a given time, the interactive components between fleets and stocks are known. The principle of the model consists to distribute, for each time interval, the fleets in each box. The allocation of fishing effort is assigned by two coefficients : "adherence" and "preference", expressing the abilities of fleets to seize the opportunities. Thus, it is possible to assess the consequences of usual management measures : quotas, mesh size, limitation of fishing effort, etc ...
Une synthèse de tous les éléments biologiques acquis précédemment et des données économiques de base est effectuée dans un modèle bio-économique. A partir d'une typologie des flottilles françaises péchant en Mer Celtique, on met en évidence les métiers (espèces + secteurs) pratiqués, ce qui permet de définir des compartiments spatio-temporels dans lesquels on connaît à un moment donné les éléments interactifs entre flottilles et stocks. Le principe du modèle consiste à distribuer, à chaque intervalle de temps les flottilles dans ces compartiments. L'allocation de l'effort est gérée par deux coefficients, adhérence et préférence, qui traduisent les capacités des flottilles à saisir les opportunités. Ainsi, il est possible d'évaluer les conséquences des mesures habituelles de gestion : quotas, maillages, limitation de l'effort, etc...

Droits : 1989 Ifremer
http://archimer.ifremer.fr/doc/1989/rapport-1564.pdf
http://archimer.ifremer.fr/doc/00000/1564/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
This paper describes an operational protocol for integrating conservation and restoration with land-use planning in islands. Conservation challenges are intensified in insular systems due to higher ecosystem vulnerability, limited spatial options, low data availability, rapid land-use change and, globally, short-term vision planning. Our operational planning protocol integrates ecological and socio-economic factors to identify the best spatial options for conserving and restoring biodiversity, inside and outside extant reserves, while minimising future land-use conflicts. Conservation and restoration targets are formulated for species, habitats and ecological processes that support biodiversity. An optimal network of priority sites is selected to achieve those targets across the landscape. The prioritisation process integrates a Conservation Costs Index to optimise conservation and restoration investments. We discuss the outcomes of the planning protocol in terms of site prioritisation, stakeholders' participation and general implications for spatial planning in insular systems. As with many islands, the study area of Réunion Island has experienced rapid urban and agricultural expansion, which threatens its unique biodiversity. Forty three per cent of the island is currently protected in a National Park but only half of this reserve network contributes to the achievement of targets. An additional 21% of land should be conserved mainly to ensure the persistence of ecological connections between the marine, terrestrial and freshwater realms. Finally we emphasize that our method doesn't substitute the land-use planning debate but is aimed to better prepare the conservation sector for negotiating future land-use allocation with other socio-economic sectors in islands
Landscape and Urban Planning

halsde-00676425
https://hal.archives-ouvertes.fr/halsde-00676425
DOI : 10.1016/j.landurbplan.2011.02.004

Éditeur(s) : HAL CCSD
Résumé : This paper presents the experimental results obtained for an automatic detection of ships in High Resolution optical satellite images. Our images are panchromatic SPOT 5 images, whose resolution is 5m per pixel. Our detection method is part of an industrial project of maritime surveillance. It is based on the Bayesian decision theory and does not need any preprocessing.The detection is composed of three stages. The first one is a pre-detection of targets that gives us candidates. The second one is a precise segmentation of each candidate. The third one is a classification of candidates into three classes : real small targets, real big targets and false alarms. The two first stages are based on the bayesian theory, using a very simple image model that leads to very fast algorithms. For now, the classification is based on multivariate decision tree. Finally, the overall results of the method are given for a set of images, as close as possible to the operational conditions and show the performances of the proposed method (detection of almost all the ships, false alarms due to crests of waves and an acceptable computing time).
Journée Nationale de Photogrammétrie et Télédétection

hal-00601854
https://hal.archives-ouvertes.fr/hal-00601854

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Ro 31-8220 is a potent protein kinase C (PKC) inhibitor belonging to the chemical class of bisindolylmaleimides (BIMs). Various PKC-independent effects of Ro 31-8220 have however been demonstrated, including inhibition of the ATP-binding cassette drug transporter breast cancer resistance protein. In the present study, we reported that the BIM also blocks activity of the solute carrier organic cation transporter (OCT) 1, involved in uptake of marketed drugs in the liver, in a PKC-independent manner. Ro 31-8220, in contrast to other pan-PKC inhibitors such as staurosporine and chelerythrine, was thus shown to cis-inhibit uptake of the reference OCT1 substrate tetraethylammonium in OCT1-transfected HEK293 cells in a concentration-dependent manner (IC50 = 0.18 μM) and without altering membrane expression of OCT1. This blockage of OCT1 was also observed in human hepatic HepaRG cells that constitutionally express OCT1. It likely occurred through a mixed mechanism of inhibition. Ro 31-8220 additionally trans-inhibited TEA uptake in OCT1-transfected HEK293 cells, which likely discards a transport of Ro 31-8220 by OCT1. Besides Ro 31-8220, 7 additional BIMs, including the PKC inhibitor LY 333531, inhibited OCT1 activity, whereas 4 other BIMs were without effect. In silico analysis of structure-activity relationships next revealed that various molecular descriptors, especially 3D-WHIM descriptors related to total size, correspond to key physico-chemical parameters for inhibition of OCT1 activity by BIMs. In addition to activity of OCT1, Ro 31-8220 inhibited those of other organic cation transporters such as multidrug and toxin extrusion protein (MATE) 1 and MATE2-K, whereas, by contrast, it stimulated that of OCT2. Taken together, these data extend the nature of cellular off-targets of the BIM Ro 31-8220 to OCT1 and other organic cation transporters, which has likely to be kept in mind when using Ro 31-8220 and other BIMs as PKC inhibitors in experimental or clinical studies
ISSN: 1932-6203

hal-01255837
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01255837
DOI : 10.1371/journal.pone.0144667
PUBMED : 26657401
PUBMEDCENTRAL : PMC4675551

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The non-classical human leukocyte antigen-G, HLA-G, plays an important role in inducing tolerance, through its immunosuppressive effects on all types of immune cells. Immune tolerance is a key issue in the liver, both in liver homeostasis and in the response to liver injury or cancer. It would therefore appear likely that HLA-G plays an important role in liver diseases. Indeed, this molecule was recently shown to be produced by mast cells in the livers of patients infected with hepatitis C virus (HCV). Furthermore, the number of HLA-G-positive mast cells was significantly associated with fibrosis progression. The generation of immune tolerance is a role common to both HLA-G, as a molecule, and the liver, as an organ. This review provides a summary of the evidence implicating HLA-G in liver diseases. In the normal liver, HLA-G transcripts can be detected, but there is no HLA-G protein. However, HLA-G protein is detectable in the liver tissues and/or plasma of patients suffering from hepatocellular carcinoma, hepatitis B or C, or visceral leishmaniasis and in liver transplant recipients. The cells responsible for producing HLA-G differ between diseases. HLA-G expression is probably induced by microenvironmental factors, such as cytokines. The expression of HLA-G receptors, such as ILT2, ILT4, and KIRD2L4, on liver cells has yet to be investigated, but these receptors have been detected on all types of immune cells, and such cells are present in liver. The tolerogenic properties of HLA-G explain its deleterious effects in cancers and its beneficial effects in transplantation. Given the key role of HLA-G in immune tolerance, new therapeutic agents targeting HLA-G could be tested for the treatment of these diseases in the future.
ISSN: 0168-8278

hal-01134468
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134468
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134468/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134468/file/Biology%20of%20the%20immunomodulatory%20molecule%20HLA-G.pdf
DOI : 10.1016/j.jhep.2015.03.007

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Microalgae in the division Haptophyta play key roles in the marine ecosystem and in global biogeochemical processes. Despite their ecological importance, knowledge on seasonal dynamics, community composition and abundance at the species level is limited due to their small cell size and few morphological features visible under the light microscope. Here, we present unique data on haptophyte seasonal diversity and dynamics from two annual cycles, with the taxonomic resolution and sampling depth obtained with high-throughput sequencing. From outer Oslofjorden, S Norway, nano- and picoplanktonic samples were collected monthly for 2years, and the haptophytes targeted by amplification of RNA/cDNA with Haptophyta-specific 18S rDNA V4 primers. We obtained 156 operational taxonomic units (OTUs), from c. 400.000 454 pyrosequencing reads, after rigorous bioinformatic filtering and clustering at 99.5%. Most OTUs represented uncultured and/or not yet 18S rDNA-sequenced species. Haptophyte OTU richness and community composition exhibited high temporal variation and significant yearly periodicity. Richness was highest in September-October (autumn) and lowest in April-May (spring). Some taxa were detected all year, such as Chrysochromulina simplex, Emiliania huxleyi and Phaeocystis cordata, whereas most calcifying coccolithophores only appeared from summer to early winter. We also revealed the seasonal dynamics of OTUs representing putative novel classes (clades HAP-3-5) or orders (clades D, E, F). Season, light and temperature accounted for 29% of the variation in OTU composition. Residual variation may be related to biotic factors, such as competition and viral infection. This study provides new, in-depth knowledge on seasonal diversity and dynamics of haptophytes in North Atlantic coastal waters. See also the Perspective by Massana
ISSN: 0962-1083

hal-01545321
https://hal.archives-ouvertes.fr/hal-01545321
DOI : 10.1111/mec.13160

Éditeur(s) : HAL CCSD
Résumé : International audience
This paper presents the early work, done in the context of the IntuiScript project, on handwriting quality analysis. This IntuiScript project aims at developing a digital workbook to help with teaching children how to handwrite. To do so, we must be able to analyse their handwriting, to evaluate if the letters are correctly written, and to detail what aspects of the child symbols – letters, numbers, and geometric forms-do not correspond to the teacher models. We use an online fuzzy model to easily build target models, and to automatically evaluate the adequacy of children letters to these reference models, with respect to different aspects: symbol shape, drawing direction and stroke order for example.
17th Biennial Conference of the International Graphonomics Society
Pointe-à-Pitre, Guadeloupe

Droits : info:eu-repo/semantics/OpenAccess
hal-01165765
https://hal.univ-antilles.fr/hal-01165765
https://hal.univ-antilles.fr/hal-01165765/document
https://hal.univ-antilles.fr/hal-01165765/file/IGS_2015_submission_4.pdf

Éditeur(s) : Inter-Research
Résumé : On the western coast of the Atlantic, according to the literature, 2 distinct groups of Semibalanus balanoides occur with a distinct interface near the Miramichi Estuary, New Brunswick, in the Gulf of St. Lawrence. On each side of this interface, the groups are characterized by clinal variations for MPI (mannose-6-phosphate isomerase) and GPI (glucose-6-phosphate isomerase). The present study was carried out to determine whether selection occurs at this interface, to establish how early in the sessile life period it occurs and to examine the selecting forces involved. Reciprocal transplant experiments of newly settled individuals to both sides of the interface were carried out. No significant differences specifically linked to source or destination were observed in growth or fecundity for the 2 groups at the sites studied for either control or transplanted individuals. However, differences in survival were observed; individuals transplanted south of the estuary showed lower survival than individuals transplanted north. An allozyme analysis of barnacle survivors for MPI and GPI, 2 alleles whose frequencies are known to vary abruptly in this region, indicated a change of allele frequency in transplanted individuals. The transplants’ allele frequencies came to resemble those of adults from target sites, while no change occurred in transplanted individuals at control sites. Taken together with previous results, our study suggests that selection occurs very early in the newly settled individuals (spat).
Marine Ecology Progress Series (0171-8630) (Inter-Research), 2002-03 , Vol. 229 , P. 173-184

Droits : Inter-Research 2002
http://archimer.ifremer.fr/doc/00048/15970/13410.pdf
DOI:10.3354/meps229173
http://archimer.ifremer.fr/doc/00048/15970/

Éditeur(s) : Soc General Microbiology
Résumé : The Plasmodium falciparum kinome includes a family of four protein kinases (Pfnek-1 to -4) related to the NIMA (never-in-mitosis) family, members of which play important roles in mitosis and meiosis in eukaryotic cells. Only one of these, Pfnek-1, which we previously characterized at the biochemical level, is expressed in asexual parasites. The other three (Pfnek-2, -3 and -4) are expressed predominantly in gametocytes, and a role for nek-2 and nek-4 in meiosis has been documented. Here we show by reverse genetics that Pfnek-1 is required for completion of the asexual cycle in red blood cells and that its expression in gametocytes in detectable by immunofluorescence in male (but not in female) gametocytes, in contrast with Pfnek-2 and Pfnek-4. This indicates that the function of Pfnek-1 is non-redundant with those of the other members of the Pfnek family and identifies Pfnek-1 as a potential target for antimalarial chemotherapy. A medium-throughput screen of a small-molecule library provides proof of concept that recombinant Pfnek-1 can be used as a target in drug discovery.
Microbiology-sgm (1350-0872) (Soc General Microbiology), 2011-10 , Vol. 157 , P. 2785-2794

Droits : 2011 SGM Printed in Great Britain / This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
http://archimer.ifremer.fr/doc/00175/28578/26965.pdf
DOI:10.1099/mic.0.049023-0
http://archimer.ifremer.fr/doc/00175/28578/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Tropical climate imposes a high level of physiological stress, which could modify the target heart rate in training load prescription, as the recommendations are often determined by maximal oxygen uptake testing in temperature-neutral laboratories. To test this hypothesis, 7 highlevel cyclists performed two randomised maximal tests in neutral (19.2±0.9 ◦C; 51.7±1.3% RH) and Tropical environment (25.8±1.1 ◦C; 63.7±2.3% RH). Neither maximal oxygen uptake nor ventilatory threshold was influenced by the environmental conditions. However, ventilation (p < 0.005) and the respiratory equivalent in O2 (p < 0.05) were significantly higher in the Tropical environment, whereas maximal power output and the time to attain maximal oxygen uptake were significantly lower (p < 0.05 for both). Moreover, the ventilatory cost of cycling (expressed in LW−1) was significantly greater in the Tropical condition (0.40±0.03 LW−1 vs. 0.32±0.05 LW−1, in Tropical vs. Neutral; condition effect: p < 0.005; condition×time: p < 0.001). Rectal temperature was influenced by neither the environmental conditions nor exercise (36.7±0.1 and 37.0±0.1 ◦Cvs. 36.8±0.1 and 37.1±0.2 ◦C, in Tropical vs. Neutral, before and after exercise) but was influenced by condition×time (p < 0.05). The heart rate (HR) values usually used for training prescription were not significantly different (154±5 bpm vs. 156±4 bpm and 172±4 bpm vs. 167±4 bpm in Tropical vs. Neutral climate, for the first and second thresholds, respectively). We concluded that the usual parameters measured during maximal exercise to establish training programs are not impaired in moderate Tropical environment. Nevertheless, the thermal stress attested by the increased ventilatory cost of cycling could have prevented the cyclists from performing a true maximal test in Tropical conditions.
ISSN: 1440-2440

hal-00703461
https://hal.univ-antilles.fr/hal-00703461
DOI : 10.1016/j.jsams.2009.11.004

Éditeur(s) : HAL CCSD
Résumé : Anti-Müllerian hormone (AMH) is a member of the TGF-ß superfamily. AMH is well known for its role in Müllerian duct regression in male fetuses. Postnatally, AMH is secreted by granulosa cells (GCs) of small growing follicles (preantral and small antral). However, despite the increasing interest of ovarian AMH in clinics, little is known on its mechanism of action and its role in female reproductive tract. My PhD project focuses on the identification of AMH function in the female reproductive tract.AMH signals through a type II transmembrane serine/threonine kinase receptor (AMHR-II) which forms a complex with a type I serine/threonine kinase receptor (ActR-IA, BMPR-IA, BMPR-IB). The type II receptor phosphorylates serine and threonine residues of type I receptor. Once activated, the type I receptor phosphorylates the receptor-regulated Smads (R-Smad1/5/8) which interact with a common partner Smad4. The Smad complex accumulates into the nucleus and regulates target gene expression. This canonical signalling pathway is regulated at different levels, in particular by co-receptors which amplify or antagonize TGF-ß family members action. The type I receptors and R-Smads involved in AMH effects on post-natal GCs remain unknown. In addition, to date, no co-receptor has been found for AMH. To define the involvement of the different type I receptors, we used siRNA technology to inactivate Acvr1, Bmpr1a and Bmpr1b in GC. In parallele, we analysed GC extracted from conditional mutant mice for Acvr1 and Bmpr1a. We found that BMPR-IA is the most important type I receptor for AMH to transduce its signal in GC. A Smad-Gal4/UAS-luciferase reporter gene technology allowed us to show that Smad1 and 5 are involved in AMH signaling pathway. Recently, new BMPs coreceptors were found, RGMs for Repulsive Guidance Molecules. There are three RGMs : RGMa, b and c. Because AMH shares with BMPs its type I receptors and R-Smad proteins, we hypothesized that they also share the same co-receptors, the RGM. We showed that RGMb was the only one expressed in GC and after siRNA transfection we demonstrated that this coreceptor is not essential for AMH to transduce its signal.To date, only few AMH target genes have been identified. Aromatase (Cyp19a1) and LH receptor (Lhcgr) are down-regulated by AMH in rat and porcine GCs. We used micro-array technology (Affymetrix) by comparing Wt and knockout immature ovaries to find new AMH target genes. This experiment evidenced that Ovgp1 and Kcnj2 are two new potential AMH target genes in the ovary.The last part of my project was to define a potential role of AMH in murine uterus. Only one study showed that AMHR-II is expressed in the mouse myometrium. We showed that Amh gene is slightly expressed in uterus but the results are not confirmed at the protein level. Using PCR-array, we found a lot of differentially expressed genes between Wt and Amh KO uterus. Therefore, AMH could regulate uterine function through the modulation of different genes located in the myometrium.
L’hormone anti-Müllerienne (AMH) est un membre de la famille TGF-β impliquée dans la différenciation du tractus reproductif mâle. Elle est aussi exprimée par les cellules de la granulosa de l’ovaire adulte. Cependant, son rôle physiologique chez la femelle n’a pas encore été entièrement établi. Mon projet de thèse a pour objectif d’élucider le(s) rôle(s) de l’AMH dans le tractus reproductif femelle. L’AMH transduit ses effets par l’intermédiaire de deux récepteurs transmembranaires sérine/thréonine kinase : un récepteur de type II qui lui est spécifique (AMHR-II) et un récepteur de type I (ActR-IA, BMPR-IA, BMPR-IB) qu’elle partage avec les BMPs. Après fixation de l’hormone sur le récepteur de type II, celui-ci recrute et phosphoryle le récepteur de type I. Ce dernier phosphoryle à son tour les Smads spécifiques (Smad1, 5 et 8) qui s’associent à la Smad commune, Smad4. L’ensemble transloque dans le noyau et en association avec des facteurs de transcription régule les gènes cibles de l’hormone. L'utilisation de souris KO conditionnelles pour les récepteurs Acvr1 et Bmpr1a et d'une technique de siRNA dirigés contre chacun des trois récepteurs de type I a permis de mettre en évidence que le récepteur BMPR-IA est un acteur essentiel de la voie de signalisation de l'AMH dans les cellules de la granulosa. Pour déterminer la ou les Smad(s) impliquées, une technique de gènes rapporteurs, Smad-Gal4/UAS-luciférase, a été utilisée. Nous avons pu montrer que les Smad1 et 5 sont importantes pour la transduction du signal de l'AMH dans les cellules de la granulosa. Récemment des corécepteurs aux BMPs, les Repulsive Guidance Molecule (RGMs), ont été mis en évidence. L’AMH partageant sa voie de signalisation avec les BMPs, nous avons cherché à déterminer si ces corécepteurs pouvaient également intervenir dans la voie de signalisation de l’AMH. Il existe 3 types de RGMs: RGMa, RGMb et RGMc. Nous avons montré en q-PCR que seul RGMb est exprimé dans les cellules de la granulosa alors que les 3 RGMs sont exprimés dans l’ovaire. L'utilisation de siRNA dirigés contre RGMb a permis de montrer que ce récepteur n'est pas nécessaire à la transduction du signal de l'AMH. Actuellement, seuls deux gènes cibles de l’AMH sont connus dans les cellules de la granulosa : l’aromatase et le récepteur LH. Nous avons réalisé des analyses de puces à ADN (ou micro-array) pour décrire de nouveaux gènes cibles de l'AMH. L'analyse des puces a permis de décrire de nouveaux gènes régulés par cette hormone tels qu’Ovgp1 ou Kcnj2. La dernière partie de mon projet visait à déterminer un rôle potentiel de l'AMH dans l'utérus. En effet, le récepteur de cette hormone est exprimé dans le myomètre utérin de souris permettant de supposer qu’elle peut agir sur cet organe. Nous avons pu mettre en évidence une expression faible du gène de l’Amh dans l’utérus. En revanche, l’expression et la localisation de la protéine restent encore à définir. Une expérience de PCR-array a permis de montrer que de nombreux gènes sont différentiellement exprimés entre l’utérus Wt et l’utérus KO Amh. Ceci indique que l’AMH jouerait un rôle sur la régulation de la fonction utérine qu’elle soit exprimée ou non dans cet organe.
https://tel.archives-ouvertes.fr/tel-00989662

Droits : info:eu-repo/semantics/OpenAccess
NNT : 2014PA11T014
tel-00989662
https://tel.archives-ouvertes.fr/tel-00989662
https://tel.archives-ouvertes.fr/tel-00989662/document
https://tel.archives-ouvertes.fr/tel-00989662/file/VA_SEDES_LAURIANE_03042014.pdf

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Children are exposed to toxic metals and metalloids via their diet and environment. Our objective was to assess the aggregate chronic exposure of children aged 3–6 years, living in France, to As, Cd, Cr, Cu, Mn, Pb, Sb, Sr, and V present in diet, tap water, air, soil and floor dust in the years 2007–2009. Dietary data came from the French Total Diet Study, while concentrations in residential tap water, soil and indoor floor dust came from the ‘Plomb-Habitat’ nationwide representative survey on children's lead exposure at home. Indoor air concentrations were assumed to be equal to outdoor air concentrations, which were retrieved from regulatory measurements networks. Human exposure factors were retrieved from literature. Data were combined with Monte Carlo simulations. Median exposures were 1.7, 0.3, 10.2, 34.1, 60.3, 0.7, 0.1, 44.3, 1.5 and 95th percentiles were 4.4, 0.5, 15.8, 61.3, 98.3, 2.5, 0.1, 111.1, 2.9 μg/kg bw/d for As, Cd, Cr, Cu, Mn, Pb, Sb, Sr, and V respectively. Dietary exposures dominate aggregate exposures, with the notable exception of Pb - for which soils and indoor floor dust ingestion contribute most at the 95th percentile. The strengths of this study are that it aggregates exposures that are often estimated separately, and uses a large amount of representative data. This assessment is limited to main diet and residential exposure, and does not take into account the relative bioavailability of compounds. These results could be used to help target prevention strategies.
ISSN: 0160-4120

inserm-01404061
http://www.hal.inserm.fr/inserm-01404061
http://www.hal.inserm.fr/inserm-01404061/document
http://www.hal.inserm.fr/inserm-01404061/file/rev1%20-%20MS%20Expo%20metals.pdf
DOI : 10.1016/j.envint.2016.10.009

Éditeur(s) : HAL CCSD
Résumé : International audience
Home health care workers interventions have been implemented in western countries to improve health status of patients with respiratory diseases especially asthma and allergic illnesses. Twenty-six controlled studies dealing with prevention and control of these diseases through home environmental interventions were reviewed. After a comprehensive description of the characteristics of these studies, the effectiveness of each intervention was then evaluated in terms of participants' compliance with the intervention program, improvement of quality of the indoor environment, and finally improvement of health outcomes, in detailed tables. Limitations and biases of the studies are also discussed. Overall, this review aims at giving a toolbox for home health care workers to target the most appropriate measures to improve health status of the patient depending on his and/or her environment and disease. Only a case-by-case approach with achievable measures will warrant the efficacy of home interventions. This review will also provide to the research community a tool to better identify targets to focus in future evaluation studies of home health care workers action.
Journal of allergy and clinical immunology. In practice

hal-01477202
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01477202
DOI : 10.1016/j.jaip.2016.07.011
PUBMED : 27665387

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: Heterogeneous patterns of malaria transmission are thought to be driven by factors including host genetics, distance to mosquito breeding sites, housing construction, and socio-behavioural characteristics. Evaluation of local transmission epidemiology to characterize malaria risk is essential for planning malaria control and elimination programmes. The use of geographical information systems (GIS) techniques has been a major asset to this approach. To assess time and space distribution of malaria disease in Bandiagara, Mali, within a transmission season, data were used from an ongoing malaria incidence study that enrolled 300 participants aged under six years old ". Methods: Children's households were georeferenced using a handheld global position system. Clinical malaria was defined as a positive blood slide for Plasmodium falciparum asexual stages associated with at least one of the following signs: headache, body aches, fever, chills and weakness. Daily rainfall was measured at the local weather station. Landscape features of Bandiagara were obtained from satellite images and field survey. QGIS™ software was used to map malaria cases, affected and non-affected children, and the number of malaria episodes per child in each block of Bandiagara. Clusters of high or low risk were identified under SaTScan W software according to a Bernoulli model. Results: From June 2009 to May 2010, 296 clinical malaria cases were recorded. Though clearly temporally related to the rains, Plasmodium falciparum occurrence persisted late in the dry season. Two " hot spots " of malaria transmission also found, notably along the Yamé River, characterized by higher than expected numbers of malaria cases, and high numbers of clinical episodes per child. Conversely, the northeastern sector of the town had fewer cases despite its proximity to a large body of standing water which was mosquito habitat. Conclusion: These results confirm the existence of a marked spatial heterogeneity of malaria transmission in Bandiagara, providing support for implementation of targeted interventions. Background Malaria is one of the leading causes of morbidity and mortality in the world, with an estimated 3.3 billion people at risk of malaria [1]. The incidence of malaria worldwide is estimated to be 216 million cases per year, with 81% of these cases occurring in sub-Saharan Africa. Malaria kills approximately 655,000 people per year; 91% of deaths occur in sub-Saharan Africa [1], mostly in children under five years of age. In Mali, West Africa, malaria represents 36.5% of consultation motives in health center, it is a leading cause of morbidity and mortality children of less than five years of age and the first reason of anaemia in pregnant women [2]. Malaria transmission is seasonal. Malaria parasite transmission and clinical disease are characterized by important microgeographic variation, often between adjacent villages, households or families [3-8]. This local heterogeneity is driven by a variety of factors including human genetics [9,10], distance to potential breeding sites [11,12], housing construction
ISSN: 1475-2875

hal-01307672
https://hal-amu.archives-ouvertes.fr/hal-01307672
https://hal-amu.archives-ouvertes.fr/hal-01307672/document
https://hal-amu.archives-ouvertes.fr/hal-01307672/file/1475-2875-12-82.pdf
DOI : 10.1186/1476-072X-2-5

Éditeur(s) : Elsevier
Résumé : In the Caribbean, snappers (Lutjanidae) and groupers (Serranidae) are often heavily exploited by artisanal or industrial fisheries. This paper analyzes the catches of an artisanal fishery selectively targeting these species with a moderate fishing pressure in the Bay Islands (Honduras), and discusses the implications on the understanding of the early stages of development of reef fisheries. Although snappers and groupers are targeted with handlines and spearguns in the whole archipelago, the differences in species diversity and size structure of the catch reveals various exploitation patterns. In most areas, a depletion is observed for the most vulnerable snapper and grouper species, and most other species are mainly exploited in their juvenile phase; clear signs of an intensification process are apparent in one of the areas. The comparison of the relative production in the Bay Islands with other reef fisheries in the Caribbean suggests that higher values may be related to moderate fishing pressure and appropriate combinations of fishing effort and selectivity. This example shows how fisheries strictly targeting a high quality resource with selective fishing techniques can be quite productive even at high levels of effort, but also that they are progressively lead to broaden their species range (sooner or later at the expense of the most vulnerable, often large-sized, species) while increasingly using less selective gears.
Fisheries Research (0165-7836) (Elsevier), 2005-06 , Vol. 73 , N. 1-2 , P. 159-169

Droits : 2004 Elsevier B.V. All rights reserved
http://archimer.ifremer.fr/doc/2005/publication-512.pdf
DOI:10.1016/j.fishres.2004.12.008
http://archimer.ifremer.fr/doc/00000/512/