Résumé : L’importance du rôle des petites agricultures dans les processus de sécurité alimentaire et de souveraineté alimentaire est de plus en plus reconnue. A Sainte-Lucie comme en Martinique, un certain nombre d’acteurs s’engagent officiellement pour une agriculture diversifiée s’appuyant sur les préceptes du développement durable. C’est en tenant compte de ce contexte qu’en partie l’idée de réaliser une étude sur la petite agriculture de Sainte-Lucie et de la Martinique a germé. Aussi, le présent travail se veut-il avant tout une contribution au diagnostic de la petite agriculture face à la modernisation des systèmes agricoles intensifs dans chacune de ces deux îles. Il s’agit de mettre à l’évidence les richesses et les insuffisances de cette petite agriculture, mais aussi les atouts et les contraintes de l’environnement. En grande partie elle prend appui sur le jardin créole, système agricole considéré par bien d’acteurs comme un fondement culturel et une base culturale. Dans chacune des deux îles, beaucoup de petits agriculteurs font de la biodiversité une composante primordiale de la gestion de leurs systèmes agricoles. Les enjeux visés ici portent sur la contribution d’une gestion optimale de cette biodiversité à la promotion des agrosystèmes paysans durables à différentes échelles spatio-temporelles. Dès lors, tout dépend des méthodes d’accompagnement des agriculteurs à mettre en œuvre. Va-t-on poursuivre avec des approches descendantes basées sur la logique de transfert ? Si non, le moment n’est-il pas venu de réunir les conditions pouvant permettre la mise en œuvre des véritables démarches de co-construction ?
The important role of small farmers in the process in food security and of food sovereignty is increasingly recognized. In St. Lucia as in Martinique, a number of actors officially committed to a diverse agriculture based on the precepts of sustainable development. It is in the light on this context that partially, the idea of a study on small holder St Lucia and Martinique germinated. Also, the present work is first and foremost a contribution to the diagnosis of small-scale agriculture to modernization of the intensive agricultural systems in each of these two islands. It is put clearly the richness and the inadequacies of this small scale-agriculture, but also the advantages and constraints of the environment. In large part it builds on the creole garden, agricultural system considered by many actors as a cultural foundation and a base of farming practices. In each of the two islands, many small farmers make biodiversity an essential component of management of their farm systems. The stakes referred here relate to the optimal management contribution of biodiversity to promote sustainable peasant agro-ecosystems at different spatial and temporal scales. Therefore, everything depends on farmer coaching methods to set up. Will we continue with down approaches based on logic of transfer ? Otherwise, isn’t time to create the conditions that could allow the development of real co-construction steps ?

http://www.theses.fr/2015AGUY0866/document

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : International audience
Sandbox experiments, using a two-dimensional and a three-dimensional approach, are used to study the deformation of margins in response to seamount subduction. Successive mechanisms of deformation are activated during the subduction of conical seamounts. First, reactivation of the frontal thrusts and compaction of the accretionary wedge is observed. Then, back thrusting and, conjugate strike-slip faulting develops above the leading slope of the subducted seamount. The basal d6collement is deflected upward in the wake of the subducting high, and a large shadow zone develops behind the seamount trailing slope. Consequently, frontal accretion is inhibited and part of the frontal margin is dragged into the subduction zone. When the main d6collement returns to its basal level in the wake of the seamount, the margin records a rapid subsidence and a new accretionary wedge develops, closing the margin reentrant. The sediments underthrusted in the wake of the seamount into the shadow zone, are underplated beneath the rear part of the accretionary wedge. Substantial shortening and thickening of the deformable seaward termination of the upper plate basement, associated with basal erosion is observed. Seamount subduction induces significant material transfer within the accretionary wedge, favors large tectonic erosion of the frontal margin and thickening of the rear part of the margin. The subduction and underplating of relatively undeformed, water-ladden sediments, associated with fluid expulsion along the fractures affecting the margin could modify the fluid pressure along the basal ddcollement. Consequently, significant variations of the effective basal friction and local mechanical coupling between the two plates could be expected around the subducting seamount.
ISSN: 0278-7407

hal-01261523
https://hal.archives-ouvertes.fr/hal-01261523
https://hal.archives-ouvertes.fr/hal-01261523/document
https://hal.archives-ouvertes.fr/hal-01261523/file/Dominguez2000-Tectonics.pdf
DOI : 10.1029/1999TC900055

Éditeur(s) : Agro Paris Tech
Résumé : P. acidilactici MA 18/5M is a dietary probiotic (BACTOCELL®) which has shown its efficiency on several terrestrial and aquatic species. The present thesis aimed to investigate more fully the effects of this probiotic on cultured penaeid shrimps. For this study, we chose a global approach by replacing the problem studied in a model of interactions between three compartments: the shrimp, its intestinal microflora and the probiotic. Having first defined how the probiotic should be administrated, we studied its effects under controlled laboratory conditions at several levels of Litopenaeus stylirostris biology: (i) Nutritional status and growth, (ii) associated bacterial microflora, and (iii) health status with respect to oxidative stress. Then the probiotic improves the growth of the shrimps and the feed conversion ratio. Our study shows that these effects are partially due to a better use of the feed, particularly of dietary carbohydrates, but also suggest an action on the metabolism, andor on the growth via a contribution of essential nutrients. On the other hand, although the intestinal bacterial communities seems little affected, we show an antagonism effect of the probiotic toward total and vibrionacea intestinal flora, as well as a decrease in the level of infection of shrimps during challenge with pathogenic V. nigripulchritudo. Beside, our works highlight that infection by this pathogen, led to a reduction in antioxidant defences, to a rise in oxidative damages, and to a release of mortalities within 48 hours. This oxidative stress following bacterial infection was better controlled by animals receiving the probiotic diet, and resistance of the shrimps to infection was significant improved. A nutritional hypothesis is proposed to explain such result, hypothesis based on a link between carbohydrate metabolism and antioxidant status. As a conclusion of these works, we can propose a first explanatory scenario for the effects of P. acidilactici on a penaeid shrimp. Finally probiotic's effects were assessed under a pilot scale in a commercial farm from New Caledonia currently affected by the "summer syndrome ". We confirm the improvements brought in term of zootechnical results, with in particular, a better resistance to this syndrome. This work was made possible thanks to a breeding method in floating cages, developed during this thesis, and therefore transferred, as an experimental tool, to the shrimp industry of New Caledonia.
Pediococcus acidilacticii MA18/5M est un probiotique bactérien (BACTOCELL®) qui a démontré son efficacité chez plusieurs espèces d'animaux terrestres et aquatiques. Cette thèse avait pour objectif d'investiguer plus en détails les effets de ce probiotique chez les crevettes pénéides d'élevage. Pour cette étude, nous avons choisi une approche globale replaçant la problématique dans un modèle d'interactions entre trois compartiments : la crevette, sa microflore intestinale et le probiotique. Après avoir défini les modalités d'administration, l'effet de P. acicilactici a été étudié, en conditions de laboratoire, chez la crevette Litopenaeus stylirostris, à différents niveaux: (i) statut nutritionnel et croissance, (ii) microflore bactérienne associée, et (iii) santé de l'animal à travers son statut antioxydant et son niveau de stress oxydant. Ainsi, le probiotique améliore la croissance des crevettes et l'indice de conversion de l'aliment. Notre étude montre que ces effets sont en partie liés à une meilleure utilisation de l'aliment, notamment des carbohydrates alimentaires, mais suggèrent aussi une action sur le métabolisme, et/ou sur la croissance via un apport de nutriments essentiels. D'autre part, bien que les communautés bactériennes intestinales semblent peu affectées, un effet d'antagonisme du probiotique vis-à-vis de la flore intestinale totale et vibrionacée a été montré, ainsi qu'une réduction du niveau d'infection des crevettes au cours de challenge avec le pathogène V. nigripulchritudo. Par ailleurs, nous mettons en évidence que l'infection par ce pathogène, conduit à un abaissement des défenses antioxydantes, à une élévation des dégâts oxydatifs, et au déclenchement de mortalités dans les 48 heures. Le probiotique atténue le niveau de ce stress oxydant, et améliore significativement la résistance des crevettes à l'infection. Une hypothèse nutritionnelle est proposée afin d'expliquer ce résultat, hypothèse reposant sur un lien entre le métabolisme des carbohydrates et le statut antioxydant. A l'issue de ces travaux, nous pouvons alors proposer un premier scénario explicatif des effets de P. acidilactici chez une crevette pénéide. Finalement le probiotique a été éprouvé à l'échelle pilote sur une ferme commerciale de Nouvelle-Calédonie affectée par le « syndrome d'été ». Nous confirmons les améliorations apportées en termes de résultats zootechniques, avec notamment une meilleure résistance au syndrome. Ce travail a été réalisé grâce une méthode d'élevage en cages flottantes, mis au point au cours de cette thèse, et qui a dés lors été transférée, comme outil expérimental, à la filière crevetticole Calédonienne.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2009/these-6814.pdf
http://archimer.ifremer.fr/doc/00000/6814/

Éditeur(s) : Mercator Ocean
Résumé : Face to the lack of data to assess precisely the spatial distribution of catches and fishing effort and for the environmental characterization of the fishing area, Ifremer has been implemented since 2005 a new project, Recopesca. It consists in fitting out a sample of voluntary fishing vessels with sensors recording data on fishing effort (and at mid-terms catches) and physical parameters such as temperature or salinity. Recopesca aims at setting up a network of sensors, for scientific purposes, to collect data and improve resources assessment and diagnostics on fisheries, and environmental data required for ecosystem-based management initiatives. The challenge was to develop sensors with no trouble for the fishermen, tough enough to be fixed up on fishing gears, self powered and autonomous. Insofar as the sample of targeted vessels intends to be representative of all the metiers and fleets, the sensors are modular and scalable to collect new data. Different sensors have been implemented: (i) a temperature-salinity sensor, able to record physical parameters, depth and duration of immersion, for passive and active gears, and (ii) a specific sensor to record number or length of passive gears. A GPS monitors the position of the vessels and the temperature or salinity profiles and series. Each sensor is equipped with a radio device transferring the data to a receiver on-board, called “concentrator” that sends the data to Ifremer central databases by GPRS. An anti-rolling weigh-scale has been developed and is currently on test to record catches per species and fishing operation. The presentation will show the first data and results of this participative approach.
Mercator Ocean - Quarterly Newsletter (Mercator Ocean), 2010-04 , N. 37 , P. 40-48

Droits : 2010 Mercator Ocean
http://archimer.ifremer.fr/doc/00024/13500/10514.pdf
http://archimer.ifremer.fr/doc/00024/13500/

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
Gravity Recovery and Climate Experiment (GRACE) satellite-derived total water storage can be obscured by glacial isostatic adjustment. In order to solve this problem for the Nelson River drainage basin in Canada, a gravity rate map from 110 months (June 2002 to October 2011) of GRACE gravity data was corrected for glacial isostatic adjustment using an independent gravity rate map derived from updated GPS vertical velocities. The GPS-based map was converted to equivalent gravity rate using a transfer function developed from GPS and absolute-g data at colocated sites. The corrected GRACE gravity rate map revealed a major positive anomaly within the drainage basin, which was independently shown by hydrological data to be due to changes in water storage. The anomaly represents a cumulative increase at its center of about 340 mm of water, reflecting a progression from extreme drought to extremely wet conditions.
ISSN: 0094-8276

hal-00940965
https://hal.archives-ouvertes.fr/hal-00940965
DOI : 10.1002/2013GL057973

Éditeur(s) : Styli 2003. Trente ans de crevetticulture en Nouvelle-Calédonie. Nouméa - Koné, 2-6 juin 2003. 2004. Gorarant C, Harache Y, Herbland A, Mugnier C. Ed. Ifremer, Actes de Colloque, n°38, pp.113-120
Résumé : Two oyster species are currently present along the French coasts : the indigenous European flat oyster (Ostrea edulis), and the Pacific cupped oyster (Crassostrea gigas), that has been introduced from Japan since the beginning of the 70ies. The flat oyster successively suffered from two protozoan diseases during the 60ies and its production decreased from 20 000 tons/year by that time to 1 500 tons/year nowadays. Consequently, the oyster production is principally (99%) based upon the Pacific oyster species with approximately 150 000 tons/year among which 90% are grown from the natural spat. However, the hatchery production of this species is developing and was estimated to 400 to 800 millions spat in 2002. Moreover, strengthened relationships between IFREMER and the 5 commercial hatcheries, that all joined the SYSAAF (Union of the French poultry, shellfish and fish farming selectors), allow to plan for new genetic breeding programs. At the end of the 80ies, IFREMER initiated a genetic breeding program for the resistance of the European flat oyster to the bonamiosis, and obtained strains more tolerant to this disease. After two generations of massal selection, molecular markers had identified a reduced genetic basis in this program. It was then reoriented to an intra-familial selection. However, we were confronted to a zootechnic problem to manage such a scheme and we compromised by an intra-cohorts of families selection scheme managed using molecular markers. The program has now reached the transfer level with experimentation at a professional scale. Concerning the Pacific cupped oyster, and in parallel with the obtaining and the study of polyploids, performance of different Asian cupped oyster strains were compared to the one introduced in France thirty years ago and currently suffering from summer mortalities. The local strain exhibited better performance, certainly based upon a good local adaptation. In other respects, although early growth is a relevant criteria for selection for growth to commercial stage, it is not to be privileged in the context of an oyster producing region with a limited food availability. Contrary, the spat summer mortality became a priority for numerous teams (genetic, physiology, pathology, ecology,...) joined in the MOREST program. The first results showed important survival differences between fullsib and halsib families. They indicate a genetic determinism to this character "survival" and promote for its selection.
Deux espèces d'huîtres sont aujourd'hui présentes sur les côtes françaises : l'huître plate européenne (Ostrea edulis), indigène, et l'huître creuse du Pacifique (Crassostrea gigas), introduite du Japon dans le début des années 1970. L'huître plate a subi coup sur coup, dans les années 1960, deux maladies dues à des protozoaires parasites et sa production est ainsi passée de 20 000 t/an dans les années 1960 à 1500 t/an aujourd'hui en France. Il en résulte que la filière ostréicole française repose quasiment exclusivement (99%) sur la production de l'huître creuse, C. gigas, avec 150 000 tonnes en 2000 dont environ 90% est issu du captage naturel. Cependant, la reproduction de cette espèce en écloserie est actuellement en expansion avec une estimation de 400 à 800 millions de naissains commercialisés en 2002. Des rapports renforcés entre l'IFREMER et les cinq écloseries françaises de la filière ostréicole, qui adhèrent toutes au SYSAAF (SYndicat des Sélectionneurs Avicoles et Aquacoles Français), permettent désormais d'envisager le développement de nouveaux programmes d'amélioration génétique. A la fin des années 1980, un programme de sélection pour la résistance de l'huître plate à la bonamiose a été initié au sein de l'IFREMER, et a permis d'obtenir des souches plus tolérantes à cette maladie. Après deux générations en sélection massale, les marqueurs moléculaires avaient mis en évidence une base génétique réduite au sein de ce programme qui s'était alors orienté vers une sélection intra-familiale. Devant la lourdeur zootechnique d'un tel schéma, un compromis a été trouvé en réalisant une sélection au sein de cohortes de familles biparentales, la gestion de ces cohortes étant permise par les analyses moléculaires. Le programme est maintenant dans sa phase de transfert, avec des expérimentations à plus grande échelle en partenariat avec la profession. En ce qui concerne l'huître creuse, et en parallèle de l'obtention et l'étude de polyploïdes, les performances de différentes souches d'huîtres creuses provenant d'Asie ont été comparées à celles de la souche introduite en France il y a trente ans, sujette à des mortalités estivales. La souche locale présente de meilleures performances, vraisemblablement grâce à une adaptation bien réalisée. Par ailleurs, bien que l'amélioration de la croissance jusqu'à la taille commerciale s'avère possible par une sélection précoce au stade larvaire, le critère de croissance n'est pas nécessairement à privilégier dans le contexte d'un bassin ostréicole disposant de ressources trophiques limitées. En revanche, les importantes mortalités rencontrées par le naissain en périodes estivales constituent le sujet d'étude prioritaire sur lequel se sont penchées de nombreuses équipes (génétique, physiologie, pathologie, écologie, ...) dans le cadre du grand défi MOREST. Les premiers résultats ont mis en évidence de fortes différences de survie entre des familles biparentales d'animaux indiquant un déterminisme génétique du caractère « survie », et encourageant dans la voie d'une sélection pour ce caractère.

Droits : 2004 Ifremer
http://archimer.ifremer.fr/doc/2003/acte-3491.pdf
http://archimer.ifremer.fr/doc/00000/3491/

Éditeur(s) : HAL CCSD HUMANA PRESS INC
Résumé : International audience
Ever since Darwin, the familiar genealogical pattern known as the Tree of Life (TOL) has been prominent in evolutionary thinking and has dominated not only systematics, but also the analysis of the units of evolution. However, recent findings indicate that the evolution of DNA, especially in prokaryotes and such DNA vehicles as viruses and plasmids, does not follow a unique tree-like pattern. Because evolutionary patterns track a greater range of processes than those captured in genealogies, genealogical patterns are in fact only a subset of a broader set of evolutionary patterns. This fact suggests that evolutionists who focus exclusively on genealogical patterns are blocked from providing a significant range of genuine evolutionary explanations. Consequently, we highlight challenges to tree-based approaches, and point the way toward more appropriate methods to study evolution (although we do not present them in technical detail). We argue that there is significant benefit in adopting wider range of models, evolutionary representations, and evolutionary explanations, based on an analysis of the full range of evolutionary processes. We introduce an ecosystem orientation into evolutionary thinking that highlights the importance of ``type 1 coalitions'' (functionally related units with genetic exchanges, aka ``friends with genetic benefits''), ``type 2 coalitions'' (functionally related units without genetic exchanges), ``communal interactions,'' and ``emergent evolutionary properties.'' On this basis, we seek to promote the study of (especially prokaryotic) evolution with dynamic evolutionary networks, which are less constrained than the TOL, and to provide new ways to analyze an expanded range of evolutionary units (genetic modules, recombined genes, plasmids, phages and prokaryotic genomes, pangenomes, microbial communities) and evolutionary processes. Finally, we discuss some of the conceptual and practical questions raised by such network-based representation.
EVOLUTIONARY GENOMICS: STATISTICAL AND COMPUTATIONAL METHODS, VOL 2

ISBN : 978-1-61779-584-8
hal-01544801
https://hal.archives-ouvertes.fr/hal-01544801
DOI : 10.1007/978-1-61779-585-5_4

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
The Ronda peridotite massif in Southern Spain shows a well-defined 'recrystallization' front that separates a large-scale partial melting domain formed at the expense of the continental lithospheric mantle from a 'preserved' lithospheric domain. To investigate the processes allowing a transient lithosphere-asthenosphere boundary to propagate in the lithospheric mantle, we performed a joint petrostructural and geochemical study of an similar to 2.5 km(2) zone extending from the melting front to the mylonites that mark the western limit of the massif. This study emphasizes a feedback between heat transfer, melt percolation, and deformation in the lithospheric mantle. Petrographical observations and geochemical data show that heterogeneous reactive percolation of melts produced in the underlying partial melting domain led to refertilization of lithospheric peridotites up to 1.5 km ahead from the melting front, producing metre-scale layering of fertile and refractory mantle rocks. Within 800 m from the front, pre-existing garnet pyroxenite layers were partially molten and the resulting melts probably contributed to the refertilization process. Detailed structural mapping and analysis of the microstructures and crystal preferred orientations highlight the relations between reactive melt transport and deformation, and the control of the temperature gradient on both processes. Parallelism between the recrystallization front, compositional boundaries, and deformation structures, as well as variations in the deformation intensity of pyroxenes and spinels, suggest syn- to late-tectonic melt transport controlled by both the deformation and the thermal gradient. Variations in the strength of olivine crystal preferred orientations as a function of the modal and chemical composition of the spinel tectonites point to a higher contribution of diffusion to deformation in the most fertile rocks, corroborating the hypothesis that deformation occurred in presence of melt. Finally, the systematic dispersion of olivine [100] and orthopyroxene [001] axes in the foliation plane suggests a dominantly transpressive deformation regime.
ISSN: 0022-3530

hal-00420904
https://hal.archives-ouvertes.fr/hal-00420904
DOI : 10.1093/petrology/egp032

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
GPS measurements adjacent to the southern Red Sea and Afar Triple Junction, indicate that the Red Sea Rift bifurcates south of 17 degrees N latitude with one branch following a continuation of the main Red Sea Rift (similar to 150 degrees Az.) and the other oriented more N-S, traversing the Danakil Depression. These two rift branches account for the full Arabia-Nubia relative motion. The partitioning of extension between rift branches varies approximately linearly along strike; north of similar to 16 degrees N latitude, extension (similar to 15 mm/yr) is all on the main Red Sea Rift while at similar to 13 degrees N, extension (similar to 20 mm/yr) has transferred completely to the Danakil Depression. The Danakil Block separates the two rifts and rotates in a counterclockwise sense with respect to Nubia at a present-day rate of 1.9 +/- 0.1 degrees/Myr around a pole located at 17.0 +/- 0.2 degrees N, 39.7 +/- 0.2 degrees E, accommodating extension along the rifts and developing the roughly triangular geometry of the Danakil Depression. Rotating the Danakil Block back in time to close the Danakil Depression, and assuming that the rotation rate with respect to Nubia has been roughly constant, the present width of the Danakil Depression is consistent with initiation of block rotation at 9.3 +/- 4 Ma, approximately coincident with the initiation of ocean spreading in the Gulf of Aden, and a concomitant similar to 70% increase in the rate of Nubia-Arabia relative motion.
ISSN: 0094-8276

hal-00475536
https://hal.archives-ouvertes.fr/hal-00475536
DOI : 10.1029/2009GL041127

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Analysis of microbial biodiversity is hampered by a lack of reference genomes from most bacteria, viruses, and algae. This necessitates either the cultivation of a restricted number of species for standard sequencing projects or the analysis of highly complex environmental DNA metagenome data. Single-cell genomics (SCG) offers a solution to this problem by constraining the studied DNA sample to an individual cell and its associated symbionts, prey, and pathogens. We used SCG to study marine heterotrophic amoebae related to Paulinella ovalis (A. Wulff) P.W. Johnson, P.E. Hargraves & J.M. Sieburth (Rhizaria). The genus Paulinella is best known for its photosynthetic members such as P.chromatophora Lauterborn that is the only case of plastid primary endosymbiosis known outside of algae and plants. Here, we studied the phagotrophic sister taxa of P.chromatophora that are related to P.ovalis and found one SCG assembly to contain -cyanobacterial DNA. These cyanobacterial contigs are presumably derived from prey. We also uncovered an associated cyanophage lineage (provisionally named phage PoL_MC2). Phylogenomic analysis of the fragmented genome assembly suggested a minimum genome size of 200Kbp for phage PoL_MC2 that encodes 179 proteins and is most closely related to Synechococcus phage S-SM2. For this phage, gene network analysis demonstrates a highly modular genome structure typical of other cyanophages. Our work demonstrates that SCG is a powerful approach for discovering algal and protist biodiversity and for elucidating biotic interactions in natural samples.
ISSN: 0022-3646

hal-01544797
https://hal.archives-ouvertes.fr/hal-01544797
DOI : 10.1111/jpy.12028

Résumé : Les biofilms électroactifs (EA) sont des consortia bactériens mono ou pluri-espèces, qui ont la faculté d’échanger des électrons provenant de leur métabolisme avec les surfaces solides conductrices des électrodes. Cette découverte est à l’origine d’un nouveau dispositif énergétique : la pile à combustible microbienne (PACM). Depuis les années 2000, la littérature scientifique sur les biofilms EA et sur les PACM explose, notamment grâce à la découverte de bactéries capables de transférer par voie directe – par des pili ou protéines transmembranaires – des électrons vers les électrodes. Plusieurs sources de bactéries EA ont été à ce jour découvertes, allant de cultures pures à des communautés bactériennes plus complexes. Ces dernières sont issues de milieux aqueux naturels (milieux marins ou d’eau douce), industriels ou urbains (effluents d’industrie, eaux usées domestiques). La plupart de ces sources bactériennes proviennent d’environnements de climat tempéré. Dans ce travail de thèse, plusieurs sols de milieux humides de la Guyane ont été identifiés comme étant de sources de bactéries EA. Les expériences menées sous potentiel d’électrode imposé et constant ont permis d’étudier l’adhésion à la surface d’électrode des biofilms EA issus de la flore endogène des milieux sélectionnés. La formation de bioanodes et de biocathodes a été possible en présence respectivement d’acétate et d’oxygène dans les milieux. Une étude par voltammétrie cyclique a mis en évidence les pics d’oxydo-réduction en lien avec les échanges électroniques du biofilm EA et de l’électrode. En optimisant la procédure de formation des biofilms EA par chronoampérométrie (surface d’électrode plus importante, apport en continu et progressif du substrat), une densité de courant maximale de 12 A/m2 et un rendement faradique de 24 % ont été obtenus. Une autre méthode pour former des biofilms EA à partir d’un milieu choisi, la mangrove, a consisté à utiliser deux prototypes de PACM : une pile à compartiment unique et à cathode à air, et une pile benthique. Dans les deux cas, les biofilms EA ont pu être formés et étudiés, complétant certaines observations faites sous potentiel imposé. La PACM benthique s’est avérée être la plus proche d’une application à grande échelle puisqu’elle a été complètement autonome : anode et cathode utilisant uniquement le milieu pour fonctionner. Elle a pu être étudiée en laboratoire comme sur le terrain.
Electroactive biofilms (EA) are mono or multi-species bacterial consortia, which have the ability to exchange electrons from their metabolism with solid surfaces of conductive electrodes. This discovery is the basis for a device of energy production: microbial fuel cell (MFC). Since the 2000s, the scientific literature on EA biofilms and MFC explodes, thanks to the discovery of bacteria that are able to transfer directly – by pili or trans-membrane proteins – electrons to electrodes. Several sources of EA bacteria were discovered to date, ranging from pure cultures to more complex bacterial communities. Those last are from natural (marine and freshwater), industrial or urban (industrial effluents, domestic wastewater) aqueous environments. However, the vast majority of these are from temperate environments.In this thesis, several wetland soils of French Guiana have been identified as sources of EA bacteria. Experiments under poised and constant electrode potential were used to examine adherence to the electrode surface of EA biofilms from the endogenous flora of selected environments. Formation of bioanodes and biocathodes was possible in the presence respectively of acetate and oxygen in the media. A study by cyclic voltammetry showed the redox peaks related to electronic exchanges between EA biofilm and electrode. By optimizing the process of EA biofilm formation by chronoamperometry (larger electrode surface, providing continuous and progressive substrate), a maximum current density of 12 A/m2 and a coulombic efficiency of 24% were obtained.Another method to form EA biofilms from a chosen medium (mangrove) was to use two MFC prototypes: a single compartment and air cathode one, and a benthic one. In both cases, the EA biofilms have been trained and studied supplementing certain observations made under poised polarization. MFC benthic proved to be the closest to a wide application since it was completely autonomous, anode and cathode only using the medium to function. It has been studied in the laboratory and in the field.

http://www.theses.fr/2012AGUY0560/document

Éditeur(s) : Elsevier Science Bv
Résumé : The timing, routing and processes of sediment transfer from the continents to the oceans at millennial time-scale are still largely unknown. The potential of turbidite systems (dominantly deposited during sea-level lowstands) to record global or regional environmental fluctuations is usually under-exploited because of the difficulty to obtain robust chronostratigraphic constraints in turbiditic deposits, and therefore to tie changes in sedimentary processes to environmental fluctuations. We were able to obtain a millennial-scale chronostratigraphy based on oxygen isotopes of the scarce foraminifera preserved in turbiditic deposits of the Rhone Turbidite System within the Western Mediterranean. Our results show that 1) objective criteria can be defined for the selection of foraminifera preserved within the pelagic intervals between the turbiditic sequences, in order to obtain a reliable isotope stratigraphy; 2) Turbidites triggered by hyperpycnal currents are described for the first time within the Rhone Turbidite System. They are related to the periods of direct fluvial connection with the canyon head (during the sea-level lowstand and early rise), and to a period of high sediment flux in relation with the massive recession of the Rhone glaciers in the Alps; 3) The lithofacies change passing from hyperpycnal to “Bouma-type” is dated at ca 19 cal. ka BP, which might correspond to an acceleration of sea-level rise (19-ka Meltwater Pulse,).
Sedimentary Geology (0037-0738) (Elsevier Science Bv), 2015-06 , Vol. 323 , P. 148-166

Droits : 2015 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/00266/37712/35730.pdf
DOI:10.1016/j.sedgeo.2015.04.009
http://archimer.ifremer.fr/doc/00266/37712/

Éditeur(s) : HAL CCSD AGU and the Geochemical Society
Résumé : International audience
New magnetotelluric and receiver transfer function studies provide insights from the upper to the lower crust of the eastern Betic Cordillera, which is deformed by large folds, normal faults, and a major transcurrent left-lateral fault, the Carboneras Fault Zone (CFZ). Receiver function analysis determines a NNW dipping Moho reaching 20 degrees that increases in depth, from 20 km south of the CFZ up to 34 km in the Sierra de Los Filabres. In addition, seismic discontinuities determined in the upper crust are interpreted as major contacts between metamorphic complexes that are detached and folded. The MT inversion model reveals a conductive zone, also representing a crustal seismic discontinuity, associated with the Alpujarride/Nevado-Filabride contact and fitting the N vergent geometry of the Sierra Alhamilla antiform. A small flexure at Moho coincides with the CFZ, as revealed by the Bouguer anomaly trend, in agreement with the receiver function results. Moreover, the Bahr strike and tipper angle at the stations placed closest to the CFZ clearly reveal the continuity of the CFZ at least down to approximately 15 km in depth, crossing all the detected crustal discontinuities up to the Moho. The lack of a clear Moho offset associated with the Carboneras Fault supports the idea that some large strike-slip faults tend to accommodate the deformation by a broadening fault zone at lower crustal levels. Its nucleation could occur at the base of a thin crust, where melting processes critically reduced the lithospheric strength during the late Miocene, to then propagate upward, reaching the topographic surface. Northward, the lithosphere comprised moderately larger strength, and the crustal discontinuities favored the development of larger folds with kilometric amplitude instead of strike-slip faults since the late Miocene.
ISSN: 1525-2027

hal-00671024
https://hal.archives-ouvertes.fr/hal-00671024
DOI : 10.1029/2010GC003274

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
S U M M A R Y Over the last decade, thermomechanical models have revealed the control of both tectonics and erosion on the morphology of continental plateaus margins. However, unravelling the specific effects of these two coupled processes has been difficult in practice. Here, to assess the control of erosion, we investigate the dynamics of the eastern and the northern borders of the Tibetan Plateau, which are characterized by a low convergence rate and a steep topographic escarpment adjacent to the Sichuan and Tarim basins, respectively. Thermomechanical modelling of continental lithosphere coupled with fluvial denudation reveals that important crustal deformation with large-scale horizontal displacements can occur, without any convergence, as a response to mass transfer due to gravitational collapse and to erosional unloading. These processes are sensitive to crustal structure, geothermal gradient as well as surface erosion. At a timescale of several million years, our results suggest that this denudation-triggered deformation exerts a primary control on the evolution of those plateau margins by counterbalancing the mass removal due to erosion and stabilizing the topographic escarpment. This finding supports a possible explanation for the morphology of the Longmen Shan, eastern Tibet, in which the paradoxical combination of persistent high-topographic gradients close to the foreland and low convergence rates can be related to the influence of erosion on deformation patterns.
ISSN: 0956-540X

hal-01577060
https://hal-amu.archives-ouvertes.fr/hal-01577060
https://hal-amu.archives-ouvertes.fr/hal-01577060/document
https://hal-amu.archives-ouvertes.fr/hal-01577060/file/godard2009erosional.pdf
DOI : 10.1111/j.1365-246X.2009.04324.x

Résumé : Penaeus stylirostris shrimp imported from Hawaii for a genetic program and kept at the Saint-Vincent research facility had progressively developed a high sensitivity to IHHNV, the etiological agent of the Infectious, Hypodermic, and Hematopoietic Necrosis. Fears were that the virus could not only be transferred to their offspring, but also that the New Caledonian strain of Penaeus stylirostris, so far considered as resistant, could become sensitive by an off balance of its hosts/pathogen equilibrium. In October 2008, a joint decision between Uprac (shimp owner), Davar (New Caledonian Animal Health Authority) and Ifremer (scientific partner) was taken to terminate all pure breed and hybrid Hawaiian populations remaining in the ponds, and to implement an intensive fallowing protocol, and controlled restocking. IHHNV was also suspected to trouble experiments carried out in the research facility (mortalities, deformities, slow growth), thus affecting research programs. Difficulties pointed out by IHHNV have triggered since 2008 some awareness on biosecurity issues at some level of the New Caledonian shrimp industry. At the St-Vincent research station, it led to a risk assessment and implementation of biosecurity procedures (fallowing, propagation, grow out and use of screened animals) which minimizes the risk of expression of IHHNV. The use of a brand new hatchery building on site also helped in resetting routine hygiene procedures during production and in controlling incoming and outgoing items (shrimp, water, feed, personnel, equipment, pests and wastes). IHHNV has not been detected on analysed samples since then nor have any symptoms been found during experimental or broodstock growout. Keeping this control alive is considered as a useful tool to lower the risk of potential health threats, to get a better understanding in case of an outbreak, and to increase the reliability of experimental results.
Les crevettes originaires d’Hawaii et conservées au LEAD St-Vincent, dans le cadre du programme d’introduction de variabilité génétique dans le cheptel Calédonien, ont progressivement montré une sensibilité importante au virus IHHNV, agent de la Nécrose Hypodermale et Hématopoïétique Infectieuse (mortalité, croissance ralentie, déformations). Les risques d’une transmission génétique de cette sensibilité à leur descendance à moyen terme, et de son développement par rupture de l’équilibre hôte/pathogène sur la souche dite « Calédonienne » jusqu’alors considérée comme résistante à l’IHHN, ont conduit à partir d’octobre 2008, avec l’accord et l’appui de l’UPRAC et de la DAVAR, à la suppression des animaux hawaiiens ou hybrides encore présents dans les bassins de terre du LEAD St-Vincent, au vide sanitaire de ces derniers, et au repeuplement contrôlé du site. D’autre part, le virus IHHN est soupçonné d’avoir pu affecter les animaux utilisés dans les expérimentations menées au LEAD-St-Vincent (mortalités, déformations, croissance réduite), influençant donc potentiellement leur bon déroulement et celui des programmes de recherches. Les obstacles soulevés par l’expression du virus IHHN ont donc initié depuis 2008 une prise de conscience et la mise en place de démarches de biosécurité dans certains points de la filière. A la station de St-Vincent, cette démarche s’est traduite par une identification/évaluation des risques et l’application de mesures (vide sanitaire, reproduction, élevage et utilisation des animaux contrôlés) chacune dotée de procédures qui regroupées, minimisent le risque d’introduction et de dissémination de l’IHHNV. La prise en main de la nouvelle structure d’écloserie sur le site de la SASV a également été mise à profit pour revoir les procédures d’hygiène de routine dans la conduite des productions et dans le contrôle des flux intrants et sortants (animaux, eau, aliments, personnel, équipement, nuisibles, déchets). Aucune détection d’IHHNV sur les échantillons prélevés n’a depuis été observée, de même qu’aucun symptôme de l’expression de cette maladie sur les animaux en élevage sur le site de Saint-Vincent. La poursuite de ces mesures est envisagée comme un outil d’aide à la minimisation des problèmes potentiels, à leur compréhension en cas d’apparition, et à la fiabilisation des résultats.

Droits : 2010 Ifremer
http://archimer.ifremer.fr/doc/00118/22949/20776.pdf
http://archimer.ifremer.fr/doc/00118/22949/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Because territoriality is energetically costly, territorial animals frequently respond less aggressively toneighbours than to strangers, a reaction known as the "dear enemy phenomenon" (DEP). The contrary,the "nasty neighbour effect" (NNE), occurs mainly for group-living species defending resource-basedterritories. We studied the relationships between supercolonies of the pest fire ant Solenopsis saevissimaand eight ant species able to live in the vicinity of its nests plus Eciton burchellii, an army ant predatorof other ants. The workers from all of the eight ant species behaved submissively when confrontedwith S. saevissima (dominant) individuals, whereas the contrary was never true. Yet, S. saevissima weresubmissive towards E. burchellii workers. Both DEP and NNE were observed for the eight ant species, withsubmissive behaviours less frequent in the case of DEP. To distinguish what is due to chemical cues fromwhat can be attributed to behaviour, we extracted cuticular compounds from all of the nine ant speciescompared and transferred them onto a number of S. saevissima workers that were then confronted withuntreated conspecifics. The cuticular compounds from three species, particularly E. burchellii, triggeredgreater aggressiveness by S. saevissima workers, while those from the other species did not.
ISSN: 0376-6357

hal-00913344
https://hal.archives-ouvertes.fr/hal-00913344
https://hal.archives-ouvertes.fr/hal-00913344/document
https://hal.archives-ouvertes.fr/hal-00913344/file/roux_10206.pdf
DOI : 10.1016/j.beproc.2013.04.014

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Although lawsonite-bearing rocks are rare in exhumed high-pressure (HP) terranes, they are considered to exert a primary role in subduction dynamics. Recent observations in natural settings have shown that fluid–rock interaction at HP conditions, including metasomatism, may lead to unusually high lawsonite amounts even in rocks that originally contained little or no lawsonite. This process may therefore bear important implications for element recycling in subduction zones. A detailed characterization of the geochemical fingerprints associated with lawsonite metasomatism is presented in this contribution. The studied rocks belong to the HP terranes of Alpine Corsica (France), which is the largest documented exposure for lawsonite metasomatism. Metasomatic lawsonite displays complex compositional zoning, including high trace element, Cr and Ti content. The trace element content is much higher compared with the average of non-metasomatic lawsonite, and is in line with the re-incorporation of large amounts of trace elements (e.g. REE, Sr, Pb, Th) in the rock during metasomatism, as shown by mass transfer calculations. Our data suggest that serpentinites represented the main fluid source for the metasomatism, with concurrent contribution of other, possibly Ca-rich lithologies, such as mafic or meta-sedimentary rocks. We propose that the breakdown of metasomatic lawsonite may contribute to the genesis of magmas and their characteristic geochemical signatures.
ISSN: 0263-4929

hal-01107395
https://hal.archives-ouvertes.fr/hal-01107395
DOI : 10.1111/jmg.12074

Éditeur(s) : HAL CCSD Seismological Society of America
Résumé : International audience
The objective of this paper is to provide a better understanding of the relationship between the microseismicity, active tectonics, and crustal structures in the southwest Yukon Territory, Canada, in order to improve seismic‐hazard assessments in this region. We utilize data from a new dense seismic array that was deployed in the southwest Yukon in the summer of 2010. Data from the new seismic array significantly improve the magnitude completeness level in the region from ML 3.0 to 1.0. We analyze 980 events ranging in magnitudes from ML 0.2 to 4.7, at depths from 0 to 35 km. Relocation analysis using the progressive multiple event location shows that seismicity is concentrated in four main areas: (1) Yakutat block northern boundary-Fairweather fault, (2) Duke River fault, (3) southern Denali fault, and (4) a previously unrecognized northeast trending cluster that may highlight a previously unknown active fault. This cluster may contribute to stress and strain transfer inland from the Yakutat block region.
ISSN: 0037-1106

hal-00940935
https://hal.archives-ouvertes.fr/hal-00940935
DOI : 10.1785/0120130068

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
A calibration method aimed at converting X-ray intensities into absolute units has been tested in different electron microprobes. The calibration requires measurement of the X-ray peak profile and measurement and calculation of the bremsstrahlung intensity emitted from a reference sample, at the energy of the X-ray line of interest. Specifically, measurements of the U M alpha and M beta lines emitted from UO2 and bremsstrahlung intensities emitted from an Al reference sample have been performed on five different instruments. The aim of the work is to determine to what accuracy X-ray intensities emitted from reference samples of actinide elements are transferable among different electron microprobes (virtual standard). The influence of the focussing geometry and different crystals and proportional counters used is analysed. Strategies to reduce the uncertainties affecting the absolute calibration of the studied lines are discussed.
ISSN: 0026-3672

hal-00411437
https://hal.archives-ouvertes.fr/hal-00411437
DOI : 10.1007/s00604-007-0856-2

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Random walk (RW) methods are recurring Monte Carlo methods used to modelconvective and diffusive transport in complex heterogeneous media. Many applications canbe found, including fluid mechanic, hydrology and chemical reactors modeling. These methodsare easy to implement, very versatile and flexible enough to become appealing for manyapplications because they generally overlook or deeply simplify the building of explicit complexmeshes required by deterministic methods. RWprovides a good physical understandingof the interactions between the space scales of heterogeneities and the transport phenomenaunder consideration. In addition, they can result in efficient upscaling methods, especiallyin the context of flow and transport in fractured media. In the present study, we review theapplications of RW to several situations that cope with diverse spatial scales and differentinsights into upscaling problems. The advantages and downsides of RW are also discussed,thus providing a few avenues for further works and applications.
ISSN: 0169-3913

insu-01308060
https://hal-insu.archives-ouvertes.fr/insu-01308060
DOI : 10.1007/s11242-016-0693-z