Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
In this paper, we describe an outcrop to characterize the effect of fracture spacing and type on larger scale effective elasticity, which is measured for the first time in-situ with a Schmidt hammer. The outcrop is dominated by lime mudstones and belongs to the deformation zone of the St Clement fault, in southern France. Our results suggest that small spacing of faults, open fractures and styolites leads to lesser effective Young's modulus, whereas small sealed fracture spacing leads to greater effective Young's modulus. These relationships are compatible with theoretical models of effective elasticity. Using Amadei and Savage (1993) approach, we define a non-linear model that relates Schmidt hammer rebound to spacing by fracture type. A hemisphere with a radius of 40 to similar to 200 cm is the rheological volume characterized by the Schmidt hammer. Results of model inversion demonstrate that variations of Schmidt hammer rebound over the outcrop can be used to estimate fracture type and stiffness. Stiffness of sealed fractures is 2-3 orders of magnitude greater than the stiffness of faults, stylolites and open fractures. This result is consistent with an increase of the rate of interseismic stress build-up of major faults with sealing of fractures in their damage zone.
ISSN: 0191-8141

hal-00669954
https://hal.archives-ouvertes.fr/hal-00669954
DOI : 10.1016/j.jsg.2011.09.009

Résumé : Palma de hasta 30 m de altura. Hojas grandes de 3 a 6 m, a veces colgantes, segmentos numerosos, linear-lanceolados de 50 a 70 cm. Flores estaminadas de 1 a 2 cm, blanco-cremosas; las pistiladas de 2.5 cm, pocas en cada inflorescencia. Fruto ovoide, ligeramente tri-anguloso, de 20 a 30 cm, pulpa blanca.
Palmier pouvant atteindre 30 m de haut. Grandes feuilles de 3 à 6 m, parfois pendantes, segments nombreux, linéaire-lancéolés de 50 à 70 cm. Fleurs avec étamines d';un blanc-crémeux, de 1 à 2 cm; fleurs femelles de 2,5 cm, peu nombreuses sur chaque inflorescence. Fruit ovoïde, légèrement tri-anguleux, de 20 à 30 cm, pulpe blanche.
Solitary palm up to 30 m tall. Trunk stout, straight or leaning, prominently ringed; leaves pinnate 3-6 m long, pinnae numerous, somewhat drooping in one plane, stiff linear acuminate 50 -70 cm long; inflorescence in the axis of the leaves; male flowers, creamy-white 0.7 – 1.3 cm x 0.5 - 0.7 cm, female flowers 20-40, globose 2.5 &ndashɑ cm in diameter; fruit ovoid, slightly triangular, 30 cm long and 20 cm in diameter with a white fleshy endosperm.
Originario de las islas del Pacífico, cultivado en las regiones tropicales del mundo.
Originaire des îles du Pacifique, cultivé dans toutes les régions tropicales du monde.
Native to the Pacific islands, cultivated in tropical regions around the wo

http://www.tramil.net/fototeca/plant33

Éditeur(s) : HAL CCSD Springer Verlag/EDP Sciences
Résumé : International audience
Background : This study examines the radial trend in wood stiffness of tropical rainforest trees. The objective was to determine if the type of growing environment (exposed plantation or dense primary forest) would have an effect on this radial trend. Methods : The axial elastic modulus of wood samples, representing a pith to bark cross-section, of six trees from several French Guianese species (two of Eperua falcata, one of Eperua grandiflora, two of Carapa procera and one of Symphonia gloubulifera) was measured using a dynamic "forced vibration" method. Results : Primary forest trees were observed to have a decrease in wood stiffness from pith to bark, whereas plantation trees, from the same genus or species, displayed a corresponding increase in wood stiffness. Juvenile wood stiffness appears to vary depending on the environment in which the tree had grown. Conclusion : We suggest that the growth strategy of primary forest trees is to produce wood resistant to selfbuckling so that the height of the canopy may be obtained with the maximum of efficiency. In contrast, the growth strategy of the trees growing in an exposed plantation is to produce low-stiffness wood, important to provide flexibility in wind. Further experiments to study the behaviour of more species, with more individuals per species, growing across a range of physical environments, are required.
ISSN: 1286-4560

hal-00644335
https://hal.archives-ouvertes.fr/hal-00644335
https://hal.archives-ouvertes.fr/hal-00644335/document
https://hal.archives-ouvertes.fr/hal-00644335/file/McLean_al.pdf
DOI : 10.1007/s13595-011-0085-z

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
This paper aims to present an overview on the influence of rheological heterogeneity and mechanical anisotropy on the deformation of continents. After briefly recapping the concept of rheological stratification of the lithosphere, we discuss two specific issues: (1) as supported by a growing body of geophysical and geological observations, crust=mantle mechanical coupling is usually efficient, especially beneath major transcurrent faults which probably crosscut the lithosphere and root within the sublithospheric mantle; and (2) in most geodynamic environments, mechanical properties of the mantle govern the tectonic behaviour of the lithosphere. Lateral rheological heterogeneity of the continental lithosphere may result from various sources, with variations in geothermal gradient being the principal one. The oldest domains of continents, the cratonic nuclei, are characterized by a relatively cold, thick, and consequently stiff lithosphere. On the other hand, rifting may also modify the thermal structure of the lithosphere. Depending on the relative stretching of the crust and upper mantle, a stiff or a weak heterogeneity may develop. Observations from rift domains suggest that rifting usually results in a larger thinning of the lithospheric mantle than of the crust, and therefore tends to generate a weak heterogeneity. Numerical models show that during continental collision, the presence of both stiff and weak rheological heterogeneities significantly influences the large-scale deformation of the continental lithosphere. They especially favour the development of lithospheric-scale strike-slip faults, which allow strain to be transferred between the heterogeneities. An heterogeneous strain partition occurs: cratons largely escape deformation, and strain tends to localize within or at the boundary of the rift basins provided compressional deformation starts before the thermal heterogeneity induced by rifting are compensated. Seismic and electrical conductivity anisotropies consistently point towards the existence of a coherent fabric in the lithospheric mantle beneath continental domains. Analysis of naturally deformed peridotites, experimental deformations and numerical simulations suggest that this fabric is developed during orogenic events and subsequently frozen in the lithospheric mantle. Because the mechanical properties of single-crystal olivine are anisotropic, i.e. dependent on the orientation of the applied forces relative to the dominant slip systems, a pervasive fabric frozen in the mantle may induce a significant mechanical anisotropy of the whole lithospheric mantle. It is suggested that this mechanical anisotropy is the source of the so-called tectonic inheritance, i.e. the systematic reactivation of ancient tectonic directions; it may especially explain preferential rift propagation and continental break-up along pre-existing orogenic belts. Thus, the deformation of continents during orogenic events results from a trade-off between tectonic forces applied at plate boundaries, plate geometry, and the intrinsic properties (rheological heterogeneity and mechanical anisotropy) of the continental plates.
ISSN: 0040-1951

hal-01389719
http://hal.univ-reunion.fr/hal-01389719
http://hal.univ-reunion.fr/hal-01389719/document
http://hal.univ-reunion.fr/hal-01389719/file/vauchez_etal_rheology_1998_hal.pdf
DOI : 10.1016/S0040-1951(98)00137-1

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Wood has a hierarchical structure involving several levels of organisation. The stiffness of wood relies on its capacity to transfer mechanical stress to its stiffest element at the lowest scale, namely crystalline cellulose. This study aims at quantifying to what extend crystalline cellulose contributes to wood stiffness depending on its moisture content. The crystal strains of cellulose were measured using X-ray diffraction on wet and dry specimens of spruce, based on a previously published methodology. The comparison between crystal strain and macroscopic strain shows that, during elastic loading, cellulose strain is lower than macroscopic strain. The means ratio of crystal/macroscopic strain amounts 0.85 for dry specimens and 0.64 for wet specimens. This strain ratio cannot be explained just by the projection effect due to the difference in orientation between cellulose microfibrils and cell wall, but results from deformation mechanisms in series with cellulose. Analysis shows that this series contribution represents a non-negligible contribution to wood compliance and is strongly moisture-dependent. This contribution amounts 9% for dry specimens and 33% for wet specimens, corresponding to a 4-fold increase in compliance for the series contribution. The origin of these strains is ascribed to mechanisms involving bending or shear strain at different scales, due to the fact that reinforcing element are neither perfectly straight nor infinitely long.
ISSN: 0266-3538

hal-01425031
https://hal.archives-ouvertes.fr/hal-01425031
https://hal.archives-ouvertes.fr/hal-01425031/document
https://hal.archives-ouvertes.fr/hal-01425031/file/Art_Almeras_al_Contribution_cellulose_moisture-dependent_elastic_behavior_2017.pdf
DOI : 10.1016/j.compscitech.2016.11.025

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Networks of localized conjugate compactive shear bands with large dihedral angles (corresponding to so-called shear-enhanced compaction bands) were generated in 3-layer plane strain finite-difference models. The central layer has elastic–plastic properties with a small positive internal friction coefficient, negative dilatancy factor, and the hardening modulus reducing during the deformation. The two other layers are elastic. The spatial organization of the networks in the central layer, resulting from the elastic–plastic instability of the 3-layer system, was shown to be dependent on the contrast of the elastic stiffness of the layers and on the friction between them. When the stiffness E of the elastic layers and/or the friction are small, the networks are rather symmetric (the bands of both orientation families are distributed homogeneously within the layer). An increase in E at high friction, results in the clustering of the bands of one orientation along a layer segment which is followed by another segment filled with the bands of the other orientation. Different network types are characterized by different band spacing which is smaller in the clustered networks. The intensity of inelastic deformation and hence of porosity reduction within the bands are also smaller in this case. The obtained band networks are very similar to those in nature where the same variability of the network organization is observed. Available geological and mechanical data confirm the conclusions from the modeling about the origin of this variability.
ISSN: 0040-1951

hal-01116201
https://hal.archives-ouvertes.fr/hal-01116201
DOI : 10.1016/j.tecto.2014.01.021

Éditeur(s) : HAL CCSD De Gruyter
Résumé :  
Experimental results are presented with single nail joints of gonfolo rose which were loaded at different grain angles and compared to results obtained by Eurocode V. A wide range of embedding strength tests was conducted. The embedding behaviour across the grain was also investigated with a specific test apparatus. The results were analysed and modelled in terms of strength. Initial loading and unloading stiffness of the timber have been taken into account. The embedment strength of the nails varied according to the angle between the direction of loading and that to the grain. This work is part of a larger research project to establish a computer program for the prediction of stiffness and limit strengths of all timber-to-timber and timber-to-steel joints.
ISSN: 0018-3830

hal-01032183
https://hal.archives-ouvertes.fr/hal-01032183
DOI : 10.1515/HF.2008.013

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Mechanical forces influence the growth and shape of virtually all tissues and organs. Recent studies show that increased cell contractibility, growth and differentiation might be normalized by modulating cell tensions. Particularly, the role of these tensions applied by the extracellular matrix during liver fibrosis could influence the hepatocarcinogenesis process. The objective of this study is to determine if 3D stiffness could influence growth and phenotype of normal and transformed hepatocytes and to integrate extracellular matrix (ECM) stiffness to tensional homeostasis. We have developed an appropriate 3D culture model: hepatic cells within three-dimensional collagen matrices with varying rigidity. Our results demonstrate that the rigidity influenced the cell phenotype and induced spheroid clusters development whereas in soft matrices, Huh7 transformed cells were less proliferative, well-spread and flattened. We confirmed that ERK1 played a predominant role over ERK2 in cisplatin-induced death, whereas ERK2 mainly controlled proliferation. As compared to 2D culture, 3D cultures are associated with epithelial markers expression. Interestingly, proliferation of normal hepatocytes was also induced in rigid gels. Furthermore, biotransformation activities are increased in 3D gels, where CYP1A2 enzyme can be highly induced/activated in primary culture of human hepatocytes embedded in the matrix. In conclusion, we demonstrated that increasing 3D rigidity could promote proliferation and spheroid developments of liver cells demonstrating that 3D collagen gels are an attractive tool for studying rigidity-dependent homeostasis of the liver cells embedded in the matrix and should be privileged for both chronic toxicological and pharmacological drug screening.
ISSN: 0730-2312

hal-01196600
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01196600
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01196600/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01196600/file/Increasing%203D%20Matrix%20Rigidity_accepted.pdf
DOI : 10.1002/jcb.25356

Éditeur(s) : HAL CCSD Elsevier
Résumé : To contribute to the understanding of how opening-mode fractures (joints) form and open or close at depth in layered rocks, we present a 2D numerical study aiming to determine whether tensile stress can develop in pre-fractured elastic multilayers submitted to biaxial compressive strain conditions. First, we investigate the role of the elastic and geometrical properties of the layers on the development of tensile stress in models with five bonded layers and containing one open fracture in the central layer. Our results indicate that, in absence of elastic contrast (in Young's modulus) between the layers, no tensile stress develops in the models. However, when the fractured layer is stiffer than the two adjacent layers directly above and below, a lobe of horizontal tensile stress develops centered on the pre-existing fracture. The creation of this tensile stress is contingent upon the partial closing of the fracture. The levels of tensile stress and the thickness of the lobe of tensile stress increase logarithmically with an increase in the elastic contrast and are systematically larger for a larger Soft/Stiff ratio (ratio of the total thickness of the soft layers with the total thickness of the stiff layers). Second, we investigate the role of fracture interaction in the development of tensile stress in models containing a pair of open fractures. We observe that the levels of tensile stress in the region between the fractures are systematically higher than those observed in identical models containing a single fracture. This increase in tensile stress is very large for small elastic contrasts between the layers but diminishes when the elastic contrast increases. Furthermore, the spacing between the pre-existing fractures plays an important role in the stress distribution in the region between them. When the fracture spacing is equal to or lower than 1.15 times the height of the fractured layer for the experimental conditions chosen, the lobes of tensile stress centered on the fractures coalesce. This results in the formation of vast areas of tensile stress in models under remote compressive loading conditions. Such tensile areas are likely to allow the initiation and propagation of subsequent opening-mode fractures. The results obtained provide new insights into the formation of joints in layered rocks in compressive environments, with important consequences on fluid flow.
ISSN: 0040-1951

hal-00407576
https://hal.archives-ouvertes.fr/hal-00407576
DOI : 10.1016/j.tecto.2006.12.003

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Functional ecology has long considered the support function as important, but its biomechanical complexity is only just being elucidated. We show here that it can be described on the basis of four biomechanical traits, two safety traits against winds (SW) and self-buckling (SB), and two motricity traits involved in sustaining an upright position, tropic motion velocity (MV) and posture control (PC). All these traits are integrated at the tree scale, combining tree size and shape together with wood properties. The assumption of trait constancy has been used to derive allometric scaling laws, but it was more recently found that observing their variations among environments and functional groups, or during ontogeny, provides more insights into adaptive syndromes of tree shape and wood properties. However, over-simplified expressions have often been used, possibly concealing key adaptive drivers. An extreme case of over-simplification is the use of wood basic density as a proxy for safety. Actually, since wood density is involved in stiffness, loads and construction costs, the impact of its variations on safety is non-trivial. Moreover, other wood features, especially the microfibril angle (MFA), are also involved. Furthermore, wood is not only stiff and strong, but it also acts as a motor for MV and PC. The relevant wood trait for that is maturation strain asymmetry. Maturation strains vary with cell wall characteristics such as MFA, rather than with wood density. Finally, the need for further studies about the ecological relevance of branching patterns, motricity traits and growth responses to mechanical loads is discussed.
ISSN: 0022-0957

hal-00909904
https://hal.archives-ouvertes.fr/hal-00909904
https://hal.archives-ouvertes.fr/hal-00909904/document
https://hal.archives-ouvertes.fr/hal-00909904/file/Integrative_biomechanics_for_tree_Fournier_al.pdf
DOI : 10.1093/jxb/ert279

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
To quantify the relationships between anisotropy. S-wave splitting and tectonics, we determined the seismic properties of lower-crustal and upper-mantle rocks outcropping in the lvrea Zone (Northem Italy). We obtained P-and S-wave seismic velocities by laboratory direct velocity measurements and/or by calculations based on the modal compositions of the rocks, the lattice preferred orientations (LPOs), and the single crystal stiffness coefficients. Measured P-and S-wave velocities (6.0-7 .5 km s-1 and 3.6-4.2 km s-1) are typical of the lower crust. The P-wave anisotropy is in the range 0-l 0%. Shear-wave birefringence is in the range 0.0-0.6 km s-1 , with typical values between 0.0 and 0.2 km s-1 • In many cases, the birefringence is clearly related to fabric elements (foliation, lineation). Mafic rocks such as anorthosite or pyroxene-bearing gabbros exhibit low P-wave anisotropies (< 5%) and low shear-wave birefringences (less than 0.1 km s-1). In contrast, the seismic properties of felsic rocks such as biotite-bearing gneisses and mafic rocks such as amphibolites display high V P anisotropy (10%) and strong birefringence (0.3 km s-1). Biotite and amphibole preferred orientations clearly control seismic anisotropy and particularly shear-wave splitting. In these rocks, maximum splitting is observed in directions parallel to the foliation with the fast split shear wave polarized parallel to the foliation plane. To have an overview of the seismic properties of this lower-crustal section at a broader scale, we calculated from our data the anisotropie seismic properties of several hypothetical samples that are perhaps more representative of the regional anisotropy than each sample individually. For instance, the average lower-crustal sample displays an anisotropy of 5.5% for P waves and a birefringence around 0.14 km s I for S waves propagating parallel to the foliation. We observe little splitting for waves propagating at high angle to the foliation.
ISSN: 0031-9201

hal-01389712
http://hal.univ-reunion.fr/hal-01389712
http://hal.univ-reunion.fr/hal-01389712/document
http://hal.univ-reunion.fr/hal-01389712/file/Barruol_Kern-PEPI96_hal.pdf
DOI : 10.1016/0031-9201(95)03124-3

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Few studies have investigated the influence of environmental conditions on the within-treevariation in Eucalyptus wood traits. The genotype and environment effects and their interactionsinfluence the wood properties. Their evaluations are crucial to estimate the genotype values andto select with efficiency. Thus, the aim of this study was to better understand the effect ofground slope and wind speed on the spatial variation of basic density (ρ), stiffness (MOE) andmicrofibril angle (MFA) of Eucalyptus wood. Wood discs cut along the trunk were sampledfrom 150 six-year-old Eucalyptus grandis x E. urophylla hybrids growing in 3 contrastinggrowing conditions influenced by ground slope and wind regime. Near Infrared (NIR)calibrations previously developed for ρ, MOE and MFA were used to predict in our discs. 2-Dplots presenting the spatial variation of predicted wood traits were compared. The higher theground slope, the greater the magnitude of radial variation in ρ, principally in the medium zoneof the trunk. The spatial variation of wood stiffness seems to be sensitive to two environmentalconditions. The MFA radial decreases were more pronounced where the wind speed was higher.At the base and top of the trees, however, there was no significant effect of growing conditionson the radial variation of the wood properties considered here.
ISSN: 0931-1890

hal-01451896
https://hal.archives-ouvertes.fr/hal-01451896
https://hal.archives-ouvertes.fr/hal-01451896/document
https://hal.archives-ouvertes.fr/hal-01451896/file/Art_Gril_al_Spatial_variation_wood_density_2016.pdf
DOI : 10.1007/s00468-015-1327-8

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Studies on tree biomechanical design usually focus on stem stiffness, resistance to breakage or uprooting, and elastic stability. Here we consider another biomechanical constraint related to the interaction between growth and gravity. Because stems are slender structures and are never perfectly symmetric, the increase in tree mass always causes bending movements. Given the current mechanical design of trees, integration of these movements over time would ultimately lead to a weeping habit unless some gravitropic correction occurs. This correction is achieved by asymmetric internal forces induced during the maturation of new wood. The long-term stability of a growing stem therefore depends on how the gravitropic correction that is generated by diameter growth balances the disturbance due to increasing self weight. General mechanical formulations based on beam theory are proposed to model these phenomena. The rates of disturbance and correction associated with a growth increment are deduced and expressed as a function of elementary traits of stem morphology, cross-section anatomy and wood properties. Evaluation of these traits using previously published data shows that the balance between the correction and the disturbance strongly depends on the efficiency of the gravitropic correction, which depends on the asymmetry of wood maturation strain, eccentric growth, and gradients in wood stiffness. By combining disturbance and correction rates, the gravitropic performance indicates the dynamics of stem bending during growth. It depends on stem biomechanical traits and dimensions. By analyzing dimensional effects, we show that the necessity for gravitropic correction might constrain stem allometric growth in the long-term. This constraint is compared to the requirement for elastic stability, showing that gravitropic performance limits the increase in height of tilted stem and branches. The performance of this function may thus limit the slenderness and lean of stems, and therefore the ability of the tree to capture light in a heterogeneous environment.
ISSN: 0022-5193

hal-00534316
https://hal.archives-ouvertes.fr/hal-00534316
DOI : 10.1016/j.jtbi.2008.10.011

Éditeur(s) : HAL CCSD
Résumé : The presence of biological matter (biofilms) in deep geological sites for storage of, for instance, toxic elements or groundwater in aquifers was clearly proved. That biomass triggers physical and chemical processes which greatly modify the durability and the sustainability of the sites. These processes, mainly from oxidative/reductive reactions, are poorly understood. This is mainly due to the fact that former studies were done at the macroscopic level far away from the micrometric scale where relevant processes induced by bacteria take place. Investigations at microscopic level are needed. Thus, we developed an experimental set-up based on the combined use of optical microscopy (transmission), atomic force microscopy (AFM) and electrical and electro-chemical AFM microscopy (EC_AFM) in order to get simultaneous information on topographic and electro-chemical processes. The first highly sensitive step was to use AFM with biological samples in liquid environment: wepresent here a study about AFM imaging of living, moving or self-immobilized bacteria, in their genuine physiological liquid medium and in true in vivo conditions. No external immobilization protocol, neither chemical nor mechanical was needed. For the first time, the native gliding movements of Gram negative Nostoc cyanobacteria upon the surface were studied by AFM. AFM height and mechanical stiffness data were simultaneously acquired. From these, mechanical parameters, inner turgor pressure and Young modulus, were derived for different bacterial species (Anabaenopsis circularis, Rhodococcus wratislaviensis). Our study revealed that AFM imaging is thus possible on moving living species. These results open a large window on new studies of both dynamical phenomena of practical and fundamental interests such as the formation of biofilms and dynamic properties of bacteria in real physiological conditions. The second delicate step was to combine AFM and optical measurements with electrical ones. We mounted a new experimental set-up coupling real-time (i) monitoring of optical properties as the optical density (OD) evolution related to bulk bacterial growth in liquid or as the counting of number of bacteria adhering on the surface of the sample as well and (ii) electrical and electrochemical measurements. Furthermore, these results will shortly be applied to the optimized monitoring of the in-situ activity of bacteria consuming oil pollutants, following this way, in real-time, the bioremediation of an oilcontaminated soil (ANR ECOTECH_BIOPHY program).
La présence de matière biologique (biofilms) dans les sites de stockage géologique profond, d'éléments toxiques ou encore de l'eau potable des aquifères est maintenant clairement démontrée. Cette biomasse est à l'origine de processus physiques et chimiques qui modifient considérablement la durabilité et la pérennité des sites concernés. Ces processus, principalement de type oxydo-réductif, sont encore mal compris. Ceci est principalement dû aux méthodes d'investigation, principalement macroscopiques, loin de l'échelle micrométrique caractéristique des bactéries. Seules des études, basées sur des méthodes d'investigation locale, peuvent apporter les informations requises. Ainsi, nous avons développé un dispositif expérimental basé sur l'utilisation combinée de la microscopie optique (en transmission), la microscopie à force atomique (AFM) et la microscopie AFM en mode électrique et électrochimique (EC_AFM) afin d'obtenir des informations simultanées sur la topographie de l'échantillon et sur les processus électrochimiques à l'échelle des bactéries. La première étape sensible consistait à utiliser l'AFM sur des échantillons biologiques en milieu liquide: nous présentons ici les résultats de l'imagerie AFM en milieu liquide de plusieurs types de bactéries dans leurs conditions physiologiques naturelles (conditions in vivo). Aucun protocole d'immobilisation, ni chimique ni mécanique, n'a été nécessaire; et pour la première fois, les mouvements de reptation de cyanobactéries Nostoc ont été étudiés par l'AFM. Les études AFM ont permis d'acquérir des données topographiques mais aussi mécaniques : nous avons pu ainsi mesurer le module d'Young, la pression de turgescence de différentes souches bactériennes (Anabaenopsis circularis, Rhodococcus wratislaviensis). Cette étude complète, a révélé que l'imagerie AFM est donc possible sur des espèces vivantes en mouvement. Ces résultats ouvrent une grande fenêtre sur de nouvelles études d'intérêts tels que la formation de biofilms et les propriétés dynamiques de bactéries dans des conditions physiologiques réelles. La deuxième étape délicate était de combiner l'AFM aux mesures optiques et électriques. Nous avons développé un nouveau dispositif expérimental permettant (i) le suivi de l'évolution de la croissance bactérienne par la mesure des propriétés optiques comme la densité optique DO (pour le développement bactérien en volume - milieu planctonique) , ou l'analyse de l'image du substrat par comptage du nombre de bactéries sur la surface de l'échantillon (biofilm), et (ii) les mesures électriques et électrochimiques. L'ensemble de ces résultats sera prochainement appliqué au développement de nouveaux outils de surveillance d'une biodépollution de terrain contaminé par les hydrocarbures, par le suivi in situ et en temps réel de l'activité de bactéries dépolluantes (ECOTECH_BIOPHY ANR).
https://tel.archives-ouvertes.fr/tel-01044714

tel-01044714
https://tel.archives-ouvertes.fr/tel-01044714
https://tel.archives-ouvertes.fr/tel-01044714/document
https://tel.archives-ouvertes.fr/tel-01044714/file/theseDhahri2013.pdf

Éditeur(s) : HAL CCSD
Résumé : International audience
A key element of the biomechanical design of trees is their ability to generate largemechanical stresses in wood at the stem periphery. This function is necessary for the tree tocontrol the orientation of its axes, and therefore to grow in height, maintain its branches at anoptimal angle or achieve adaptive reorientations (Fournier et al., 2013). This “maturationstress” appears in wood fibres during their cellular maturation when their secondary cell wallis thickening. In hardwoods, the stress asymmetry is generated by the formation of specificfibres with a very high tensile growth stress on the upper side of the inclined axis. Theresulting tension wood has microstructural features highly different from the normal wood.Tension wood in almost all temperate species has a specific gelatinous cell wall layer, calledG-layer, that is not lignified and highly mesoporous (Chang et al., 2015). It has been shownrecently, at the macroscopic scale, that cellulose tension appears during maturation and issynchronous with the development of this specific layer (Clair et al., 2011). Tropical species,like simarouba, are able to generate lignified tension wood fibres. The mechanisms of stressgeneration in tension wood fibres are not yet well known. The aim of our study is tounderstand these mechanisms and to know the spatial and temporal kinetics of the differentcell wall layers stiffening during maturation, in parallel with tensile stress generation.Contact-Resonance Atomic Force Microscopy, CR-AFM, was used here to address thisquestion on embedded samples (Arnould and Arinero, 2015) using dual resonance frequencytracking (DRFT, Rodriguez et al., 2007). Change in the contact modulus between, and within,each layer of the cell wall has been measured on several radial lines of developing fibres atdifferent steps. In order to understand the obtained results, and to estimate the sensitivity ofthe AFM indentation-like technique to the different cell wall components stiffness, an elasticanisotropic indentation model was used (Vlassak et al., 2003; Jäger et al., 2011). Finally,these mechanical measurements have been compared to topochemical data obtained on thesame fibres.
8th Plant Biomechanics International Conference
Nagoya, Japan

hal-01436113
https://hal.archives-ouvertes.fr/hal-01436113

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
To quantify the seismic properties of lower crustal rocks and to better constrain the origin of the lower crustal seismic reflectivity, we determined the complete 3-D seismic properties of a lower crustal section. Eight representative samples of the main lithologic and structural units outcropping in the Val Sesia (Ivrea zone) were studied in detail. The seismic velocities were calculated using the single crystal stiffness coefficients and the lattice preferred orientation (LPO) of each mineral in all samples. The 21 stiffness coefficients characterizing the elastic behaviour of each rock are determined. Mafic and ultramafic rocks such as pyroxenite and pyroxene-bearing gabbros display complex shear wave properties. These rocks are weakly birefringent (maximum 0.1 kms-1) and it is difficult to find consistent relationships between the seismic properties and the rock structure. On the other hand, seismic properties of deformed felsic rocks are essentially controlled by mica. They display strong S-wave birefringence (0.3 km s-1) and relatively high Vp anisotropy (7.6 per cent). Amphibole also strongly influences the rock birefringence patterns. For both kind of rocks, the foliation is highly birefringent and the fast polarized shear wave is systematically oriented parallel to the foliation. We show that the number of mineral phases in the rock strongly controls the anisotropy. The seismic anisotropy has a complex role in the P-wave reflectivity. Compared to the isotropic case, anisotropy enhances the reflection coefficient for about 60 per cent of the possible lithological interfaces. For 40 per cent of the interfaces, the reflection coefficient is much lower when one considers the medium as anisotropic.
ISSN: 0956-540X

hal-01389122
http://hal.univ-reunion.fr/hal-01389122
http://hal.univ-reunion.fr/hal-01389122/document
http://hal.univ-reunion.fr/hal-01389122/file/Barruol_3D_velocities_GJI1993.pdf
DOI : 10.1111/j.1365-246X.1993.tb01519.x

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : L’article propose une réflexion sur le positionnement touristique de la Martinique confrontée à une rude concurrence régionale. L’analyse porte sur les potentialités de cette petite île montagneuse (1128 km2) en matière d’écotourisme forestier. Cette nouvelle orientation touristique doit permettre d’enrichir une offre touristique qui se limite encore aux simples pratiques balnéaires. L’écotourisme peut aussi être un outil de gestion des ressources forestière et plus généralement il est susceptible de soutenir des activités dans les secteurs montagneux les moins développés de la Martinique.
The article is an evaluation of the tourist situation in Martinique now facing stiff regional competition. The analysis focuses on the potential of this small mountainous island (1128 km2) in the area of forest ecotourism. This new focus is meant to enhance a tourist potential which is still largely limited to the seaside and its attractions. Ecotourism can also be a tool not only for forest resource management but also a means for promoting development initiatives in the less developed mountainous regions of Martinique.
Martinique

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.440
http://journals.openedition.org/etudescaribeennes/440

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
The paper is motivated by the long term safety analysis of the CO 2 geological storage. We present a methodology for the assessment of the ge-omechanical impact of progressive rock dissolution. The method is based on the use of X-ray tomography and the numerical dissolution technique. The influence of evolution of the microstructure on the macroscopic properties of the rock is analysed by using periodic homogenization method. The numerical computations show progressive degradation of all components of the stiffness (orthotropic) tensor. Moreover, the evolution of associated mass transfer properties (as tortuosity and conductivity tensors), by using the periodic homogenization method, is also calculated. The correlation between the mechanical parameters and the transfer properties during the dissolution pro-cess is presented. The results show that the highest increase of the hydraulic conductivity (in direction Y) is not associated with the highest decrease of Young modulus in this direction. Moreover, the highest decrease of Young modulus (in the direction X) is not associated with percolation in this direction. Finally, an incremental law to calculate settlement, in case of a rock with evolving microstructure, is proposed. The solution of the macroscopic settlement problem under constant stress and drained conditions showed that the geomechanical effects of the rock dissolution are rather limited.
ISSN: 0363-9061

hal-01104204
https://hal.archives-ouvertes.fr/hal-01104204
https://hal.archives-ouvertes.fr/hal-01104204/document
https://hal.archives-ouvertes.fr/hal-01104204/file/Numerical_computations_rock_dissolution_Wojtacki_al_2014.pdf
DOI : 10.1002/nag.2316

Éditeur(s) : HAL CCSD
Résumé : International audience
The prevalence of bone deformities, particularly linked with mineral deficiency, is an important issue for fish production. Juvenile triploid rainbow trout (Oncorhynchus mykiss) were fed a low-phosphorus (P) diet for 27 weeks (60 to 630 g body mass). At study termination, 24.9% of the fish fed the low-P diet displayed homogeneous biconcave vertebrae (deformed vertebrae phenotype), while 5.5% displayed normal vertebral phenotypes for the entire experiment. The aim of our study was to characterize the deformed phenotype and identify the putative genes involved in the appearance of P deficiency-induced deformities. Both P status and biomechanical measurements showed that deformed vertebrae were significantly less mineralized (55.0 ± 0.4 and 59.4 ± 0.5,% ash DM, for deformed and normal vertebrae, respectively) resulting in a lower stiffness (80.3 ± 9.0 and 140.2 ± 6.3 N/mm, for deformed and normal phenotypes, respectively). The bone profiles based on μCT observations showed no difference in the osteoclastic resorption while no difference in matrix production was observed between deformed (total bone area 5442.0 ± 110.1 μm2) and normal vertebrae (total bone area 5931.2 ± 249.8 μm2) in this study. Consequently, the lower P content rather results from a reduced degree of mineralization in the deformed phenotype. Finally, we quantified differential gene expression between deformed vertebrae (pronounced biconcave) and normal phenotype by employing deep RNA-sequencing and mapping against a reference bone transcriptome for rainbow trout. In total, 1289 genes were differentially expressed. Among them, in deformed fish we observed that BGLAP, MGP and NOG, an inhibitor of BMP signalling pathway, were up-regulated while COL11a1 was down-regulated. These genes are central actors involved in the reduced degree of mineralization triggering vertebral deformities. These results will further the understanding of P deficiency-induced deformities; hence providing new tools for improved P management in production settings
Journal of Next Generation Sequencing & Applications

hal-01545768
https://hal.archives-ouvertes.fr/hal-01545768
DOI : 10.4172/2469-9853.1000112

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Recent global kinematic studies reveal that most of the trenches roll back but a significant number of them advance toward the upper plate. Those advancing trenches are mostly located in the Western Pacific and correspond to the subduction of very old, Mesozoic oceanic lithosphere. While retreating trenches are commonly explained by the slab pull action of the descending lithosphere, the origin of advancing trenches is still debated. Since this relationship is dependent upon the adopted reference frame, we select region where geological studies show the variability of trench migration style with time. The Izu-Bonin-Mariana (IBM) region represents a key example. The detailed reconstruction of the trench migration of the IBM subducting system reveals that after a long episode of asymmetric rollback, the IBM trench recently started advancing. We propose that this change from retreating to advancing trench mode results from the subduction of progressively older and stiffer lithospheric material. We test this hypothesis by means of two-dimensional (2-D) numerical models, reproducing the effects of the lithospheric aging during subduction. The result of our numerical tests shows that the entrance of old and stiff lithosphere forces the trench to advance because the increasing stiffness of the slab prevents the slab to unbend once it has subducted. We adapt this physical result to the IBM evolution, showing that the switch from trench retreat to trench advance can reconcile the shape of IBM slab as previously suggested. Finally we discuss the possibility that the subduction of the Cretaceous lithosphere might have triggered the change of the motion of the Pacific plate around 5 Ma.
ISSN: 0012-821X

hal-00455988
https://hal.archives-ouvertes.fr/hal-00455988
DOI : 10.1016/j.epsl.2009.09.042