Éditeur(s) : HAL CCSD
Résumé : International audience
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest.
Molecular Human Reproduction

hal-01647081
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081
DOI : 10.1093/molehr/gax054
PUBMED : 29040674

Résumé : La problématique migratoire dans l’ultrapériphérie de l’Union européenne constitue un angle d’approche peu commun dans les études européennes. Espace de réflexion et de concertation qui cherche constamment à s’affirmer, l’ultrapériphérie européenne s’est, pour la première fois de son existence, penchée sur la thématique des flux migratoires. L’intérêt de cette situation sans précédent s’est confirmé lorsque la Commission européenne a officiellement décidé à travers sa communication du 12 septembre 2007 [COM(2007)507] intitulée « Stratégie pour les régions ultrapériphériques : bilan et perspectives », de prêter attention à la situation des régions ultrapériphériques (RUP) face au phénomène d’immigration. Si la plupart des travaux en la matière concernent les espaces nationaux ou transnationaux, rares sont ceux qui traitent d’un espace transrégional aussi éclaté géographiquement et dans sa composition juridico-institutionnelle.L’objet de la thèse porte ainsi sur la problématisation migratoire dans l’ultrapériphérie et l’étude de la fenêtre d’opportunité déclenchée par la communication de 2007. Ce qu’il s’agit d’observer, ce sont les conséquences de cette inscription à l’agenda européen pour l’espace ultrapériphérique sur cette thématique, à savoir la forme d’un processus d’élaboration d’un référentiel européen ultrapériphérique. Pour cela, il est nécessaire de comprendre les paramètres nationaux, régionaux, européens et ultrapériphériques qui englobent cet évènement.Cette étude de politiques publiques prend pour point de départ la notion d’européanisation définie de façon interactionnelle, et débouche sur un cadre théorique basé sur la Théorie du choix rationnel et l’Approche cognitive et normative. A l’aide de la méthode comparative, elle tente de confronter deux régions ultrapériphériques sélectionnées à cet effet : une région française, la Guadeloupe, et une région espagnole, les Iles Canaries. Deux archipels aux réalités juridiques, politiques et sociales différentes, ayant manifesté une inquiétude au sujet du phénomène d’immigration sur leur territoire dans la période précédant la prise en charge de 2007.L’analyse a mis en avant l’enchevêtrement de niveaux d’action publique assez complexe auquel sont confrontées ces régions. La diversité au sein de l’ultrapériphérie complique l’élaboration de bases communes au sujet de la question migratoire. Un processus d’européanisation directe dans l’ultrapériphérie a bien été lancé en 2007, mais reste à développer.L’objectif de la thèse est donc d’apporter des réponses aux difficultés observées, en menant une analyse assez précise de l’évènement de 2007. La démarche s’effectue en deux temps. D’une part, une analyse des paramètres internes (nationaux et régionaux) pouvant conditionner le processus étudié. D’autre part, un décryptage des racines conceptuelles et des premières observations de terrain pour comprendre la teneur du processus.
TraductionDésactiver la traduction instantanéeMigration into the outermost regions of the European Union is an angle unusual approach in European studies. Space for reflection and dialogue that constantly seeks to assert, Outermost Europe has for the first time in its existence, addressed the theme of migration. The advantage of this unprecedented situation is confirmed when the European Commission formally decided through its communication of 12 September 2007 [COM (2007) 507] entitled "Strategy for the outermost regions: achievements and perspectives", pay attention the situation of the outermost regions (OR) face to the immigration phenomenon. If most of the work on the subject relate to national or transnational, few areas are those that deal with cross-regional space also broke geographically and in its legal and institutionnelle.L'objet composition of the thesis and deals with migration in problematisation outermost regions and the study of the window of opportunity triggered by the communication of 2007. what is observed, it is the consequences of this inscription on the European agenda for the outermost space on this theme , namely as a process of developing a European outermost repository. For this it is necessary to understand the national parameters, regional, European and outermost encompassing évènement.Cette this policy study takes as its starting point the notion of Europeanization set of interactional way, and results in a theoretical framework based on Theory of rational choice and cognitive and normative approach. Using the comparative method, she tries to confront two outermost regions selected for this purpose: a French region, Guadeloupe, and one in Spain, the Canary Islands. Two archipelagos legal realities, different political and social, have expressed a concern about the phenomenon of immigration on their territory in the period preceding the care 2007.L'analyse highlighted the tangle of levels rather complex prosecution faced by these regions. The diversity of the outermost regions complicates the common foundations for development on the migration issue. A direct Europeanization process in the outermost regions has been launched in 2007, but remains développer.L'objectif of the thesis is to provide answers to the difficulties observed, leading a fairly accurate analysis of the event 2007. The process is carried out in two stages. On the one hand, an analysis of internal parameters (national and regional) that can condition the studied process. On the other hand, a decryption conceptual roots and the first field observations to understand the content of the process

http://www.theses.fr/2015AGUY0826/document

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Background: Squamous cell carcinoma (SCC) is considered the most frequent skin cancer in black people. Its incidence is not known in the Afro-Caribbean population.Objective: To assess the incidence of SCC in Guadeloupe, the largest island of the Lesser Antilles (405 000 inhabitants, mostly black people of African and European descent). The second objective was to characterize clinical and histological patterns of SCC occurring in the Afro-Caribbean community.Methods: This retrospective study was conducted over an 11-year period (2000-2010). Data regarding 723 histological confirmed cases of SCC identified using the three Guadeloupean pathology laboratories computerized databases were retrieved from the records of 551 patients. Private practice dermatologists and general practitioners were contacted to obtain any missing data. Results: The annual age-adjusted incidence of SCC was 15 per 100.000 residents in Guadeloupe. In the Afro-Caribbean community, SCC had a greater size (i.e.: 2.8±2.8 cm versus 1.5±1.0 cm, p<0.001), was more often located on the anogenital area (i.e. :48/79-60.8% versus 14/320-4.4%, p<0.001) in association with an underlying dermatosis due to HPV infection (15/71- 21.1% versus 3/366, 0.8%, p<0.001) and led more frequently to metastasis (13/84-15.5% versus 10/366-2.7%, p<0.001) and/or fatal evolution (11/83-13.3% versus 7/365-1.9%, p<0.001). Conclusions: The results of this original study, which first estimated the incidence of SCC in West Indies, suggest that anogenital examination should be routinely performed in skin cancer screening of Afro-Caribbean people in order to detect the presence of SCC at an early stage.
ISSN: 0926-9959

inserm-01513116
http://www.hal.inserm.fr/inserm-01513116
http://www.hal.inserm.fr/inserm-01513116/document
http://www.hal.inserm.fr/inserm-01513116/file/2017%2C%20Cordel%20-%20Squamous%20cell%20carcinoma%20in%20the%20Afro-Caribbean%20community.pdf
http://www.hal.inserm.fr/inserm-01513116/file/CordeletalJ%20Eur%20Acad%20Dermatol%20Venereol2017.pdf

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Transmission electron microscopy observations on olivine crystals deformed at moderate (≤1273 K) temperature evidenced dislocations interactions explaining the hardening observed in the experiments, but also recovery mechanisms by the absorption or emission of point defects. Thus we investigate the possibility that, at geological strain-rates, these recovery processes allow steady-state deformation by dislocation creep at low to moderate temperatures in the lithospheric mantle. We test this hypothesis using a 2.5-D dislocation dynamics (DD) model, which combines dislocation glide and recovery by climb. This model shows that diffusion-controlled recovery processes allow for steady-state deformation by dislocation creep in the lithospheric mantle at stresses <500 MPa. For stresses of 50–200 MPa, steady-state strain-rates of 10−15 s−110−15 s−1 may be attained at temperatures as low as 900 K. Fitting of the DD model produces a flow law, which represents a lower bound for the lithospheric mantle strength, since the models describe the deformation of an olivine single crystal in an easy slip orientation. Comparison of strain-rates and Moho temperatures inferred for different geodynamic environments and the predictions of this model-based flow law implies, nevertheless, that, except in incipient rifts, most of the observed deformation may be produced by stress levels ≤200 MPa, consistent with those inferred to be produced by convection. This convergence suggests that the present models, which explicitly calculate the time-dependent dislocation dynamics, may provide a correct first order estimate of the mechanical behaviour of the lithospheric mantle, which cannot be derived directly from any existing data.
ISSN: 0012-821X

hal-01277178
https://hal.archives-ouvertes.fr/hal-01277178
https://hal.archives-ouvertes.fr/hal-01277178/document
https://hal.archives-ouvertes.fr/hal-01277178/file/Boioli.pdf
DOI : 10.1016/j.epsl.2015.10.012

Éditeur(s) : ISTPM
Résumé : Crayfishing is not particularly organized in the French islands as their population is not fond of large crustaceans. However, young crayfishes, about the size of the European scampi are ordered by rich Creoles for receptions or family gatherings. But lobsters, heavier than 0.750 to 1 kilo are eaten only by Europeans.Any fisherman who is asked to provide 10 kilos of crayfish catches them very quickly with a brass snare or a hook placed underneath the rocks. Some artisans gather the crustaceans in large rectangular baskets made of wire mesh and called "gardes", to wait for the potential customer. There is no specific pot for this type of fishing whereas Creole wicker makers worked hard on the creation of tools both complex and difficult to make to catch small morays; a fish which does not have a great commercial value.Consequently, it is impossible to precisely assess the importance of the exploitable large crustacean stock based on the catches of the Creole fishermen. If the performances of some crayfish boats from Douarnenez, using Mauritanian nets on the banks of St-Martin, can be considered as extremely satisfactory, it is not the case of other attempts. A whole study has to be undertaken. This note is only the beginning of a larger work focusing on the natural history of the crayfishes and Spanish lobsters in the waters of the arc of the Petites Antilles.
La pêche des langoustes n'est pas spécialement organisée dans les îles françaises, la population n'étant que peu friande de gros crustacés. Toutefois de jeunes langoustes, de la taille de la langoustine européenne sont commandées aux pêcheurs par les riches créoles, à l'occasion de réceptions ou de fêtes familiales; mais les « homards») dépassant le poids de 0,750 à 1 kilo sont consommés seulement par les Européens. Le pêcheur. auquel on demande de fournir 10 kilos de langoustes, les capture rapidement au collet de laiton ou au crochet sous les cayes, Certains artisans rassemblent les crustacés dans de grandes nasses rectangulaires de grillage métallique, appelées « gardes », en attendant l'acheteur éventuel. Il n'existe pas de type de casier spécial à cette pêche, alors que les vanniers créoles se sont ingéniés, par exemple, à créer des engins compliqués et difficiles à construire pour capturer les petites murènes appelées « moringues » qui n'ont pourtant que peu de valeur commerciale. Il est donc impossible de se faire une idée précise de l'importance du stock de gros crustacés exploitable en se basant sur les apports des pêcheurs créoles. Si les rendements de certains langoustiers douarnenistes, travaillant au filet mauritanien sur les bancs de St-Martin, ont pu être considérés comme extrêmement satisfaisants, il n'en est pas de même à propos d'autres tentatives. Il y a là toute une étude à entreprendre. Cette note n'est que le début d'un travail concernant l'histoire naturelle des langoustes et scyllares des eaux de l'arc des Petites Antilles. [OCR NON CONTRÔLE]
Revue des Travaux de l'Institut des Pêches Maritimes (0035-2276) (ISTPM), 1958-03 , Vol. 22 , N. 1 , P. 105-114

Droits : Ifremer
http://archimer.ifremer.fr/doc/1958/publication-4567.pdf
http://archimer.ifremer.fr/doc/00000/4567/

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : International audience
Observations from deep boreholes at several locations worldwide, laboratory measurements of frictional strength on quartzo-feldspathic materials, and earthquake focal mechanisms indicate that crustal faults are strong (apparent friction μ ≥ 0.6). However, friction experiments on phyllosilicate-rich rocks and some geophysical data have demonstrated that some major faults are considerably weaker. This weakness is commonly considered to be characteristic of mature faults in which rocks are altered by prolonged deformation and fluid-rock interaction (i.e., San Andreas, Zuccale, and Nankai Faults). In contrast, in this study we document fault weakening occurring along a marly shear zone in its infancy (<30 m displacement). Geochemical mass balance calculation and microstructural data show that a massive calcite departure (up to 50 vol %) from the fault rocks facilitated the concentration and reorganization of weak phyllosilicate minerals along the shear surfaces. Friction experiments carried out on intact foliated samples of host marls and fault rocks demonstrated that this structural reorganization lead to a significant fault weakening and that the incipient structure has strength and slip behavior comparable to that of the major weak faults previously documented. These results indicate that some faults, especially those nucleating in lithologies rich of both clays and high-solubility minerals (such as calcite), might experience rapid mineralogical and structural alteration and become weak even in the early stages of their activity.
ISSN: 0278-7407

hal-01211481
https://hal.archives-ouvertes.fr/hal-01211481
DOI : 10.1002/2014TC003716

Éditeur(s) : Wiley-blackwell Publishing, Inc
Résumé : Oceanic larvae of the European (Anguilla anguilla) and American (A. rostrata) eels have to cross the Atlantic Ocean from the Sargasso Sea to European or North American coasts before entering continental habitats. In some European rivers, eel recruitment is now < 1% of levels in the 1980s. A better understanding of the effects of anthropogenic pressures and environmental fluctuations on eel larvae and subsequent recruitment is a prerequisite to build efficient management plans. The present paper provides insight into the critical oceanic phase of the eel life cycle with a focus on the duration of the larval migration whose estimates varies between 7 months and more than 2 years in both species. Does this range correspond to a natural variability in larval duration or does it stem from methodological artefacts? We first review the different methods used to estimate the duration of larval migration and critically describe their possible sources of misinterpretation. We then evaluate the consistency of these methods with the current knowledge about the ecology and physiology of eel larvae and the physical oceanography. While a moderate discrepancy in migration duration was found between methods for the American eel, the discrepancy was large in the European eel. In this species, otolith microstructure studies indicated migration durations between 7 and 9 months, while other methods pointed to durations of about 2 years. We show that estimates in favour of a long migration duration seem more robust to methodological caveats than methods estimating short durations of migration.
Fish And Fisheries (1467-2960) (Wiley-blackwell Publishing, Inc), 2010-09 , Vol. 11 , N. 3 , P. 289-306

Droits : 2010 Blackwell Publishing Ltd.
http://archimer.ifremer.fr/doc/00011/12208/9033.pdf
DOI:10.1111/j.1467-2979.2010.00362.x
http://archimer.ifremer.fr/doc/00011/12208/

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
BACKGROUND: Sampling design is a key issue when establishing species inventories and characterizing habitats within highly heterogeneous landscapes. Sampling efforts in such environments may be constrained and many field studies only rely on subjective and/or qualitative approaches to design collection strategy. The region of Cacao, in French Guiana, provides an excellent study site to understand the presence and abundance of Anopheles mosquitoes, their species dynamics and the transmission risk of malaria across various environments. We propose an objective methodology to define a stratified sampling design. Following thorough environmental characterization, a factorial analysis of mixed groups allows the data to be reduced and non-collinear principal components to be identified while balancing the influences of the different environmental factors. Such components defined new variables which could then be used in a robust k-means clustering procedure. Then, we identified five clusters that corresponded to our sampling strata and selected sampling sites in each stratum. RESULTS: We validated our method by comparing the species overlap of entomological collections from selected sites and the environmental similarities of the same sites. The Morisita index was significantly correlated (Pearson linear correlation) with environmental similarity based on i) the balanced environmental variable groups considered jointly (p = 0.001) and ii) land cover/use (p-value<0.001). The Jaccard index was significantly correlated with land cover/use-based environmental similarity (p-value = 0.001). CONCLUSIONS: The results validate our sampling approach. Land cover/use maps (based on high spatial resolution satellite images) were shown to be particularly useful when studying the presence, density and diversity of Anopheles mosquitoes at local scales and in very heterogeneous landscapes.
BMC Ecology

pasteur-00914104
https://hal-riip.archives-ouvertes.fr/pasteur-00914104
https://hal-riip.archives-ouvertes.fr/pasteur-00914104/document
DOI : 10.1186/1472-6785-13-45
PUBMED : 24289184

Éditeur(s) : HAL CCSD American Mosquito Control Association
Résumé : Research on natural insecticides has intensified with the spread of resistance to chemicals among insects, particularly disease vectors. To evaluate compounds, the World Health Organization (WHO) has published standardized procedures. However, those may be excessively compound-consuming when it comes to assessing the activity of natural extracts and pure compounds isolated in limited amount. As part of our work on the discovery of new mosquito larvicides from Amazonian plants, we developed a compound-saving assay in 5-ml glass tubes instead of WHO larval 100-m1 cups. Comparing activity of synthetic and natural chemicals validated the glass tube assay. Raw data, lethal doses that kill 50% (LD50) and 90% (LD90) at 24 and 48 h, were highly correlated (0.68 < R-2 < 0.96, P < 0.001, Pearson test) between cups and tubes. It was also established that 10 tubes (N = 50 larvae) provided the same level of sensitivity as 20 tubes (N= 100). This method proved suitable for rapid screening of natural extracts and molecules, identifying active compounds using 10 times less material than in the WHO protocol.
ISSN: 8756-971X

hal-01602207
https://hal.archives-ouvertes.fr/hal-01602207
DOI : 10.2987/16-6613.1
PRODINRA : 394297
PUBMED : 28206865

Éditeur(s) : Neidine editeurs
Résumé : This article calls back first of all a set of facts relative to the main stakes considering sea and ocean so much on the economic point of view, in the scientific technological and innovative fields, in the governance aspects as also in the culture and international prestige ones. Then the article synthesizes several of the elements of the European study on the blue growth, the established priorities, the results of the study and its recommendations. The case of New Caledonia is then analyzed which shows that considering the 11 economic priorities identified by the European Union, 10 interest or may interest this ultra-marine country. A focus is proposed on a second European study which concerns the implementation of a Blue Society (Sea for Society Project) and in synthesis and perspectives the authors wonder about the possibilities and the profits which New Caledonia could take advantage of the implementation of a maritime and marine strategy based on the principles of blue growth and blue society.
Cet article rappelle tout d’abord un ensemble de faits relatifs aux grands enjeux de la maritimisation tant au plan de l’économie, de la science, de la technologie et de l’innovation, de la gouvernance comme également de la culture et du rayonnement international. Ensuite l’article synthétise plusieurs des éléments de l’étude européenne sur la croissance bleue, les priorités établies, les résultats de l’étude et ses recommandations. Le cas de la Nouvelle-Calédonie est alors abordé qui montre que sur les 11 priorités économiques identifiées par l’Europe, 10 intéressent ou sont susceptible d’intéresser ce pays ultra-marin. Un focus est proposé sur une seconde étude européenne qui concerne la mise en place d’une Société Bleue et en synthèse et perspectives les auteurs s’interrogent sur les possibilités et bénéfices que la Nouvelle-Calédonie pourrait tirer de la mise en place d’une stratégie maritime et marine basée sur les principes de croissance et de société bleues.
Tai Kona (2269-7535) (Neidine editeurs), 2013-11 , N. 5 , P. 12-35

Droits : Tai Kona
http://archimer.ifremer.fr/doc/00174/28505/26854.pdf
http://archimer.ifremer.fr/doc/00174/28505/

Résumé : Cette these porte sur une analyse de politique publique dans un champ d'action internationale, la cooperation au developpement. l'etude s'interesse particulierement au cas de la construction de la politique communautaire de cooperation au developpement, et de son affirmation visa- vis des politiques nationales europeennes. pour ce faire, cette these analyse d'un point de vue qualitatif les ressorts de l'action de l'union europeenne, a la fois pour penser le developpement dans la caraïbe, et pour penser et faire l'europe. de l'autonomisation d'une politique a l'harmonisation des politiques nationales par le bas, c'est une veritable recomposition du systeme d'acteurs qui s'opere. ce renforcement de l'integration europeenne, dans un domaine d'action partage, par le biais d'outils renouveles de l'action publique, dessine alors progressivement les premices et les contours du cadre legislatif qui a ete mis en place par le traite de lisbonne en 2009
This dissertation analyses a public policy in an international field of action: the development cooperation. the study focuses in particular on the emergence of the european community policy of development cooperation and its assertion towards european national policies. using a qualitative methodology, this research aims to improve the understanding of the motivation and the processes at stake within the european union. the field analysis has led to observe both how the development policy in the caribbean ras been thought and built, and how this process may rave an impact on the european integration process itself. from the empowerment of a policy to the harmonisation of national policies, this process carries also consequences on the system of actors, which ras been reconstructed by those changes. as a result, the european integration process has been strengthened in a field where the competence is generally shared between the eu and its member states. bringing out some new or renewed tools of public action, this outlines also some of the signs or provisions of the forthcoming legal and political framework, set up by the 2009 lisbon treaty

http://www.theses.fr/2012AGUY0574/document

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : In the age of European exploration, terra incognita referred to lands that were largely unknown to Europeans. In the world of contemporary tourism, tourism incognita refers to the lands and experiences that are largely unknown to the global mass tourism industry (tourism cognita). Tourism incognita includes specialty niche tourisms that operate on the edge and beyond of mass tourism, as well as the entire world of non-tourism places. Examples of tourism cognita and tourism incognita that are discussed include: Dubai and Oman’s Musandam Peninsula, Cambodia’s Angkor Wat and the Tonle Sap, and the scuba diving destination of Manado on the Indonesian island of Sulawesi. Tourism incognita is shown to be vital to the success of the tourism economy because the places and experiences of tourism incognita are part of the imagination that drives the decision and desire to travel to a distant land.
À l'ère de l'exploration européenne, la terra incognita faisait référence aux terres alors largement inconnues des Européens. Dans le monde du tourisme contemporain, le tourisme incognita se renvoie aux terres et aux expériences qui sont en grande partie inconnues de l'industrie du tourisme de masse globale (tourisme cognita). Tourisme incognita comprend des créneaux touristiques spécialisés qui opèrent sur les marges du tourisme de masse, ainsi que l'ensemble du monde dela non-lieu touristique. Des exemples de tourisme et le tourisme cognita incognita traités sont: Dubaï et Oman Musandam Peninsula, Angkor wat au Cambodge et du Tonlé Sap et la destination de Manado plongée sous-marine sur l'île indonésienne de Sulawesi. Tourisme incognita est révélée déterminante pour le succès de l'économie touristique parce que les lieux et les expériences de tourisme incognita font partie de l'imaginaire qui entraîne la décision et le désir de voyager dans un pays lointain.
Cambodge
Dubaï
Indonésie
Oman

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.5232
http://journals.openedition.org/etudescaribeennes/5232

Éditeur(s) : HAL CCSD
Résumé : International audience
Background: We examined the association between exposure during pregnancy to trihalomethanes, the most common water disinfection by-products, and birth outcomes in a European cohort study (Health Impacts of Long-Term Exposure to Disinfection By-Products in Drinking Water). We took into account exposure through different water uses, measures of water toxicity, and genetic susceptibility. Methods: We enrolled 14,005 mothers (2002-2010) and their children from France, Greece, Lithuania, Spain, and the UK. Information on lifestyle- and water-related activities was recorded. We ascertained residential concentrations of trihalomethanes through regulatory records and ad hoc sampling campaigns and estimated route-specific trihalomethane uptake by trimester and for whole pregnancy. We examined single nucleotide polymorphisms and copy number variants in disinfection by-product metabolizing genes in nested case-control studies. Results: Average levels of trihalomethanes ranged from around 10 μg/L to above the regulatory limits in the EU of 100 μg/L between centers. There was no association between birth weight and total trihalomethane exposure during pregnancy (β = 2.2 g in birth weight per 10 μg/L of trihalomethane, 95% confidence interval = 3.3, 7.6). Birth weight was not associated with exposure through different routes or with specific trihalomethane species. Exposure to trihalomethanes was not associated with low birth weight (odds ratio [OR] per 10 μg/L = 1.02, 95% confidence interval = 0.95, 1.10), small-for-gestational age (OR = 0.99, 0.94, 1.03) and preterm births (OR = 0.98, 0.9, 1.05). We found no gene-environment interactions for mother or child polymorphisms in relation to preterm birth or small-for-gestational age. Conclusions: In this large European study, we found no association between birth outcomes and trihalomethane exposures during pregnancy in the total population or in potentially genetically susceptible subgroups. © 2016 Wolters Kluwer Health, Inc.
Epidemiology

hal-01392115
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01392115
DOI : 10.1097/EDE.0000000000000544
PUBMED : 27468006

Éditeur(s) : Gauthier-Villars
Résumé : This review addresses the problem of recently introduced plants in the Mediterranean in order to analyse their vectors, the dispersal processes and the possible ecological and economic consequences. Ninety-five cases (94 macroscopic algae and one seagrass) are listed and discussed. Three major routes of access into the Mediterranean are indicated: shipping, the Suez canal and aqua-culture. The recent introduction of Caulerpa taxifolia along our coasts has called attention to a new potential source: aquaria. Among the successful acclimatizations, cold-temperate Pacific and Atlantic species remain confined to the north of the Mediterranean (Gulf of Lions, Adriatic), whereas some tropical or sub-tropical plants colonize large areas. Some introduced algae proliferate to the detriment of the indigenous Mediterranean vegetation. In contrast with some terrestrial introductions, no population crash has been observed in the Mediterranean, although some notable fluctuations seem to occur in coastal lagoons. From an economic point of view, the two activities most affected by this phenomenon are aquaculture and fishing. With the gradual disappearance of European frontiers, the worldwide development of marine cultures and the multiplication of giant Seaworlds, the transfer of live marine organisms will become routine and the risk of new introductions will considerably increase in the Mediterranean. Only international legislation, established by the countries of the littoral, will be able to control these problems.
Oceanologica Acta (0399-1784) (Gauthier-Villars), 1994 , Vol. 17 , N. 1 , P. 1-23

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00098/20879/18493.pdf
http://archimer.ifremer.fr/doc/00098/20879/

Éditeur(s) : Montpellier 1
Résumé : Understanding bioaccumulation mechanisms of persistent organic pollutants (POPs) is a major issue for environmental risk assessment. In this work, polychlorinated biphenyls (PCBs), a model contaminant family, has been studied in the European hake (Merluccius merluccius), a high trophic level species from the Gulf of Lions. High concentrations of PCBs were measured in hake from this area compared to those encountered in the population of the Bay of Biscay. PCB biomagnification was highlighted along its food web from marine phytoplankton, suggesting a strong influence of diet on contamination levels. The role of other biological processes was also apprehended in order to understand the high variability of observed levels. Thus, growth, sex, and also the degree of sexual maturity appear as major factors explaining the measured concentrations in fish. A PCB bioaccumulation model in the hake, based on the Dynamic Energy Budget theory (DEB), was developed. This approach is based on a bioenergetic model, reproducing the main physiological functions of an organism (feeding, growth and reproduction), coupled with a kinetic model of contaminants. The growth and evolution of contamination levels of several PCB congeners during the life of male and female hakes is satisfactorily simulated, both in the Gulf of Lions and in the Bay of Biscay, validating the processes taken into account. This combined modelling approach appears well adapted to study the fate of contaminants in fish, and paves the way for a realistic understanding of their effects on major biological functions of fish.
La compréhension des mécanismes de bioaccumulation des contaminants organiques persistants (POP) est un enjeu majeur pour l'évaluation des risques environnementaux. Lors de ce travail, le devenir d'une famille de contaminants modèles, les polychlorobiphényles (PCB), a été étudié chez une espèce de rang trophique élevé du golfe du Lion, le merlu Européen (Merluccius merluccius). Des concentrations élevées en PCB ont été mesurées dans les merlus de ce secteur en comparaison à celles rencontrées dans la population du golfe de Gascogne. La mise en évidence d'une bioamplification des PCB le long de son réseau trophique, depuis le phytoplancton marin, suggère une influence importante de l'alimentation sur les niveaux de contamination. Le rôle d'autres processus biologiques a également été appréhendé afin de comprendre la forte variabilité des niveaux observés. Ainsi, la croissance, le sexe, mais également le degré de maturité sexuel apparaissent comme des facteurs explicatifs majeurs des concentrations en PCB mesurées dans les poissons. Un modèle de bioaccumulation des PCB chez le merlu, basé sur la théorie de Budget d'Energie Dynamique (DEB), a été développé. Il s'agit d'une approche basée sur le couplage d'un modèle bioénergétique, reproduisant les principales fonctions physiologiques de l'organisme (nutrition, croissance et reproduction), avec un modèle de cinétique des contaminants. La croissance et l'évolution des niveaux de contamination de plusieurs congénères de PCB au cours de la vie des merlus mâles et femelles est simulée de manière satisfaisante, tant dans le golfe de Gascogne que dans le golfe du Lion, validant ainsi les processus pris en compte. Cette approche combinée de modélisation apparaît tout à fait adaptée à l'étude du devenir des contaminants chez les poissons, et ouvre la voie à une compréhension réaliste de leurs effets sur les grandes fonctions biologiques de ces organismes.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2008/these-6490.pdf
http://archimer.ifremer.fr/doc/00000/6490/

Éditeur(s) : HAL CCSD Elsevier
Résumé : We report a preliminary analysis on the relationships between drainage basin structure and genetic structure of populations of the European vector of fasciolosis, Galba truncatula. In the study area, 251 snails belonging to 12 populations were collected along different ditches of a same river network. Each snail was genotyped at six variable microsatellite loci. Our results show that all sample sites are characterized by a low level of polymorphism and a very high and significant heterozygote deficiency. Our data reveal a significant genetic differentiation, even at a small scale, and failed to delimit clear patterns of isolation by euclidian distance. Our study shows that genetic differentiation significantly increases with hydrographic distance along the streams (p < 0.002), in consistence with the hypothesis that dispersion along the stream is dependent on the direction of water flow. This study shows that relationships can exist between the organization of the hydrological network and population biology of a disease vector, which has strong potential applications to drainage network management issues.
ISSN: 1567-1348

hal-00496374
https://hal.archives-ouvertes.fr/hal-00496374
DOI : 10.1016/j.meegid.2010.01.005

Éditeur(s) : Wiley-Blackwell
Résumé : The Climate Forecast System Reanalysis (CFSR) provides a wealth of information spanning 1979–2009 for investigation of ocean wind and wave climate. Preprocessing of the data is necessary to remove the dominant seasonal signals and to create time series of semimonthly averaged wind speed and significant wave height over a 0.5° global grid. We perform an empirical orthogonal function (EOF) analysis to extract the dominant space-time patterns. The results for the three major ocean basins show strong zonal structures in the winds and saturation of the swells corroborating prior works with various data sets. We reexamine the CFSR data in the frequency domain to identify periodic signals associated with published climate indices. The Fourier transform of the preprocessed time series generates spectra ranging from 1 month to 15 years period for an EOF analysis. The results demonstrate the spatial structures and periods of climate phenomena. The Arctic Oscillation dominates the Atlantic basin with a broad range of intra-annual signals off the European coasts. The Indian and Pacific Oceans are strongly influenced by inter-annual cycles of the El Niño Southern Oscillation (ENSO) and the Antarctica Oscillation. The Indian Ocean also has strong intra-annual components ranging from 50 to 80 days period. The ENSO proves to be a ubiquitous signal around the globe, and in particular, saturates the Pacific with strong influences in the equatorial region and the Southern Hemisphere Westerlies. A commonality of all basins is that the magnitude and the spatial structure of the intra-annual and inter-annual signals are similar suggesting a wide range of periods in each of the climate cycles examined.
Journal of Geophysical Research: Oceans (21699275) (Wiley-Blackwell), 2014-08 , Vol. 119 , N. 8 , P. 5563-5584

Droits : 2014. American Geophysical Union. All Rights Reserved.
http://archimer.ifremer.fr/doc/00210/32149/30606.pdf
http://archimer.ifremer.fr/doc/00210/32149/30607.pdf
DOI:10.1002/2013JC009729
http://archimer.ifremer.fr/doc/00210/32149/

Résumé : L’outre-mer comprend des régions, des pays et des territoires aux statuts divers et au passé hérité en grande partie des grandes puissances européennes, ce qui les a placés dans une communauté d’intérêts avec les Etats auxquels ils sont rattachés mais aussi avec l’Union européenne (UE).Néanmoins, ces régions, pays et territoires n’ont eu cesse de reconstruire entre eux des liens historiques et culturels et de tisser des relations avec l’ensemble de l’outre-mer afin de présenter une cohérence d’ensemble dans leur négociation avec l’Union européenne.Les régions ultrapériphériques appliquent le droit communautaire tandis que les pays et territoires d’outre-mer, situés en dehors du territoire communautaire, connaissent un régime d’association avec l’Union européenne.Le Danemark, l’Espagne, la France, les Pays-Bas, le Portugal et le Royaume-Uni ont procédé aux réformes constitutionnelles autorisant de multiples évolutions statutaires dans le respect du droit à l’autodétermination des populations.Forts d’une évolution statutaire et d’un développement économique et social continu, les régions, pays et territoires d’outre-mer souhaitent maintenant faire valoir leurs droits et défendre leurs intérêts au niveau européen et sur la scène internationale.La globalisation des politiques les incite à se regrouper au sein d’institutions internationales. Le rattachement de ces territoires à des Etats de droit leur a ouvert la voie à des systèmes juridiques bien structurés mais aussi aux valeurs européennes. Leur ultrapériphérité les a placés dans une situation géopolitique qui les a fait accéder aux relations internationales.L’objet de cette thèse est de démontrer l’interdépendance entre ces différents ordres juridiques et l’influence du droit européen et du droit international sur l’évolution statutaire de l’outre-mer vers davantage d’autonomie, mais aussi de responsabilisation et de prise en charge de son développement au moyen d’une implication dans son environnement régional, d’une coopération inter-régionale ou transnationale et d’une participation aux travaux des organisations internationales.
The Overseas regions, countries and territories present various statuses inherited mainly from the major European Powers which placed them in a relationship of a common interest not only with their mother countries but also with the European Union (EU).However, these territories have continuously re-built between them historical and cultural links, and weaved relationship within the entire Overseas to appear as a constituted whole while negotiating with the EU.The outermost regions (OR) apply Community Law while the Overseas Countries and territories (OCT) situated outside the territory of the Community, have Association Arrangements with the EU.Denmark, France, Netherlands, Portugal, Spain and United Kingdom have proceeded to necessary constitutional reforms to allow numerous articles amendments in respect of right to self-determination of peoples.At the light of statutory amendments and of a continuous economic and social development, the Outermost regions (OR) and the Overseas Countries and Territories (OCT), now wish to assert their rights and to defend their interests at European and International level.Globalisation of policies encourages OR and OCT to gather within International bodies. The rule of law of the related countries opens them to well-structured legal systems and to European values. The outermost geopolitical localisation makes them to have access to international relations.The purpose of this thesis is to demonstrate the interdependence between these different legal systems and the impact of European and International Law on the statutory amendments of the Overseas towards more autonomy, but also liabilities and involvement on their own development by using their regional environment, inter-regional and transnational cooperation and taking part at the works of international organisations.

http://www.theses.fr/2014AGUY0812

Éditeur(s) : Pêche thonière et dispositifs de concentration de poissons, Caribbean-Martinique, 15-19 Oct 1999
Résumé : Since the beginning of 1980, the Martinique fishermen have suffered a deep crisis. The traditional ways of fishing, based on benthic fish exploitation, have seen the decreasing of their profitability due to the over-fishing of the narrow continental shelf offering few resources. The pelagic fishery had become the professional main source of income. This fishery equally encounter difficulties: denied rights of access in the EEZ of Caribbean islands, expensive costs of exploitation. The disposal of FADs offers new ways to the Martinique fishermen. In 1999, it was decided the disposal of a onetworko of 30 permanent FADs around Martinique. The mooring of these FADs must be followed by legal and economic adapted measures to reach the desired aim: the development of local fishery economy through a sustainable fishing practice. Therefore, the French authorities have decided to make the regional fishermen committee responsible for the management of FADs.This committee is the only one to be entitled to moor and to deliver licences to FADs. This committee, gathering all the fishing industry representatives, has regarding the french law the right of mooring and delivering licences to the FADs access, collecting fishery statistics. These data should fund research to improve the management of the fishery, care and replace FADs. This programas realisation, as well as durability, need adequate financial planning. It has been included in the European commission financing program. This plans success will ensure the increase of the fishermen income, maintain and develop employment.Original Abstract: Apres une periode d'experimentation lancee localement au debut des annees quatre-vingt, les premiers resultats ont ete suffisamment probants pour conduire les professionnels a souhaiter l'implantation de dispositifs de concentration de poissons (dcp) en Martinique ou a les implanter eux-memes pour la capture des dorades coryphenes, des thons noirs, de l'albacore ou du listao. Le colloque international sur la peche thoniere et les dispositifs de concentration de poissons organise du 15 au 19 octobre 1999 en Martinique donne l'occasion de revenir sur le contexte, l'interet et les modalites de developpement de la technique des dcp en Martinique.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00042/15305/12639.pdf
http://archimer.ifremer.fr/doc/00042/15305/

Éditeur(s) : HAL CCSD Seismological Society of America
Résumé : International audience
Abstract The continuous record of large surface-rupturing earthquakes along the Dead Sea fault brings unprecedented insights for paleoseismic and archaeoseismic research. In most recent studies, paleoseismic trenching documents the late Holocene faulting activity, while tectonic geomorphology addresses the long-term behavior (>10 ka), with a tendency to smooth the effect of individual earthquake rupture events (Mw >7). Here, we combine historical, archaeological, and paleoseismic investigations to build a consolidated catalog of destructive surface-rupturing earthquakes for the last 14 ka along the left-lateral Jordan Valley fault segment. The 120- km-long fault segment limited to the north and the south by major pull-apart basins (the Hula and the Dead Sea, respectively) is mapped in detail and shows five subsegments with narrow stepovers (width < 3 km). We conducted quantitative geomorphology along the fault, measured more than 20 offset drainages, excavated four trenches at two sites, and investigated archaeological sites with seismic damage in the Jordan Valley. Our results in paleoseismic trenching with 28 radiocarbon datings and the archaeoseismology at Tell Saydiyeh, supplemented with a rich historical seismic record, document 12 surface-rupturing events along the fault segment with a mean interval of ∼1160 yr and an average 5 mm=yr slip rate for the last 25 ka. The most complete part of the catalog indicates recurrence intervals that vary from 280 yr to 1500 yr, with a median value of 790 yr, and suggests an episodic behavior for the Jordan Valley fault. Our study allows a better constraint of the seismic cycle and related short-term variations (late Holocene) versus long-term behavior (Holocene and late Pleistocene) of a major continental transform fault.
ISSN: 0037-1106

hal-00747401
https://hal.archives-ouvertes.fr/hal-00747401
DOI : 10.1785/0120100097